CPNE8 ORF clone pFN21AE0033

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0033

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Clone info.

Species

human

Clone Name

pFN21AE0033 Sequence (with vector) Restriction Map

Product ID

FHC10712

Flexi Clone PID

FXC10712

ORF Sequence

DNA sequence (1692 bp)
Protein sequence (564 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527319]

Gene

KIEE0033

Symbol

CPNE8

Description

copine VIII

Original Clone ID

oc00733

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1692 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 564 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8733.1 0 99.8 CPNE8 CCDS2574.2 4.4e-179 78.2 CPNE9 CCDS77695.1 1.1e-160 78.1 CPNE9 CCDS4825.1 1.9e-147 77.5 CPNE5 CCDS10774.1 3.5e-110 51.5 CPNE2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000008 30 116 PF00168 C2 calcium-dependent membrane targeting IPR000008 164 248 PF00168 C2 calcium-dependent membrane targeting IPR010734 329 472 PF07002 Copine HMMSmart IPR000008 24 132 SM00239 C2 calcium-dependent membrane targeting IPR000008 157 264 SM00239 C2 calcium-dependent membrane targeting IPR002035 307 505 SM00327 von Willebrand factor ProfileScan IPR018029 30 117 PS50004 C2 membrane targeting protein IPR018029 166 249 PS50004 C2 membrane targeting protein IPR002035 309 510 PS50234 von Willebrand factor
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065990 593 604209 77.5 98.6 XP_005249304 610 604209 75.3 98.6 XP_011513071 653 604209 79.7 88.1 XP_011513073 632 604209 78.7 82.4 XP_011513072 632 604209 78.7 82.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0033 Download

>KIEE0033  1692 bp
ATGGACAGCCGCTACAACAGCACTGCGGGCATCGGGGACTTGAACCAGCTGAGCGCTGCC
ATCCCGGCCACGCGGGTGGAGGTGTCCGTGTCCTGCAGAAATCTTCTTGACAGAGACACA
TTTTCTAAATCTGATCCAATTTGTGTCTTATATGTACAAGGAGTTGGAAATAAAGAATGG
AGAGAGTTTGGAAGAACTGAAGTAATTGATAATACTTTAAATCCTGATTTTGTAAGAAAG
TTTATTCTGGACTACTTTTTTGAAGAAAGAGAGAATCTTCGTTTTGACTTGTATGATGTT
GATTCAAAGAGCCCCAACTTATCCAAACATGACTTTCTGGGACAAGTGTTTTGTACATTG
GGAGAGATCGTTGGTTCACAGGGAAGTCGCCTGGAAAAACCAATAGTAGGAATTCCAGGG
AAGAAATGTGGTACAATCATACTTACAGCAGAGGAATTAAACTGTTGCAGGGATGCCGTT
TTGATGCAATTTTGTGCGAACAAATTGGACAAGAAGGACTTCTTTGGAAAATCAGATCCT
TTCCTTGTATTTTATCGAAGTAATGAAGATGGCAGTTTTACAATTTGTCACAAGACAGAA
GTTGTCAAAAACACTCTAAATCCAGTATGGCAAGCATTCAAGATCTCAGTCAGAGCATTA
TGTAATGGAGACTATGACAGAACAATCAAAGTAGAGGTGTATGACTGGGACCGAGATGGA
AGTCATGATTTCATTGGAGAATTTACAACAAGCTATAGGGAACTTTCTAGAGGGCAGTCA
CAATTCAACGTATATGAGGTGGTGAATCCCAAAAAGAAAGGAAAAAAGAAAAAATATACT
AATTCTGGAACAGTAACTTTACTCTCTTTCTTGGTAGAAACAGAAGTTTCATTCCTTGAC
TACATTAAGGGAGGGACGCAAATCAATTTCACAGTGGCTATTGATTTTACAGCATCAAAC
GGCAACCCTGCTCAGCCCACTTCCCCCCACTATATGAATCCTTACCAACTGAATGCCTAT
GGTATGGCACTAAAAGCAGTGGGAGAAATTGTTCAAGATTATGACAGTGATAAAATGTTT
CCAGCTCTAGGATTTGGTGCAAAACTGCCTCCAGATGGAAGGATATCTCACGAATTTGCT
TTGAATGGGAATCCTCAAAACCCCTACTGTGATGGCATTGAGGGGGTCATGGAGGCTTAT
TACAGGAGTCTGAAATCTGTACAACTATATGGGCCCACCAACTTTGCTCCTGTAATTAAT
CATGTAGCAAGATATGCTTCTTCTGTAAAGGATGGCTCCCAGTATTTTGTGCTTCTGATT
GTTACAGATGGTGTTATCTCAGATATGGCCCAGACTAAGGAGTCCATAGTTAATGCCTCA
AAACTTCCAATGTCAATAATTATAGTAGGTGTTGGACCAGCAGAATTTGATGCAATGGTC
GAATTGGATGGAGATGATGTAAGAGTCTCCTCTAGAGGAAAATATGCTGAAAGAGACATT
GTGCAGTTTGTGCCATTCAGGGATTATATTGACAGAAGTGGAAACCACATACTGAGCATG
GCTAGATTGGCTAAAGATGTCCTAGCTGAGATCCCTGAGCAGTTTCTCTCCTATATGAGA
GCCCGAGGAATCAAGCCATCACCTGCGCCTCCCCCATACACCCCACCTACACATGTGTTA
CAGACTCAAATA

ORF Protein

Cloned ORF protein sequence for pFN21AE0033 Download

>KIEE0033  564 aa
MDSRYNSTAGIGDLNQLSAAIPATRVEVSVSCRNLLDRDTFSKSDPICVLYVQGVGNKEW
REFGRTEVIDNTLNPDFVRKFILDYFFEERENLRFDLYDVDSKSPNLSKHDFLGQVFCTL
GEIVGSQGSRLEKPIVGIPGKKCGTIILTAEELNCCRDAVLMQFCANKLDKKDFFGKSDP
FLVFYRSNEDGSFTICHKTEVVKNTLNPVWQAFKISVRALCNGDYDRTIKVEVYDWDRDG
SHDFIGEFTTSYRELSRGQSQFNVYEVVNPKKKGKKKKYTNSGTVTLLSFLVETEVSFLD
YIKGGTQINFTVAIDFTASNGNPAQPTSPHYMNPYQLNAYGMALKAVGEIVQDYDSDKMF
PALGFGAKLPPDGRISHEFALNGNPQNPYCDGIEGVMEAYYRSLKSVQLYGPTNFAPVIN
HVARYASSVKDGSQYFVLLIVTDGVISDMAQTKESIVNASKLPMSIIIVGVGPAEFDAMV
ELDGDDVRVSSRGKYAERDIVQFVPFRDYIDRSGNHILSMARLAKDVLAEIPEQFLSYMR
ARGIKPSPAPPPYTPPTHVLQTQI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0033 Download

>pFN21AE0033  6397 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACAGCCGCTACAACAGCACTGCGGGCATCGG
GGACTTGAACCAGCTGAGCGCTGCCATCCCGGCCACGCGGGTGGAGGTGTCCGTGTCCTG
CAGAAATCTTCTTGACAGAGACACATTTTCTAAATCTGATCCAATTTGTGTCTTATATGT
ACAAGGAGTTGGAAATAAAGAATGGAGAGAGTTTGGAAGAACTGAAGTAATTGATAATAC
TTTAAATCCTGATTTTGTAAGAAAGTTTATTCTGGACTACTTTTTTGAAGAAAGAGAGAA
TCTTCGTTTTGACTTGTATGATGTTGATTCAAAGAGCCCCAACTTATCCAAACATGACTT
TCTGGGACAAGTGTTTTGTACATTGGGAGAGATCGTTGGTTCACAGGGAAGTCGCCTGGA
AAAACCAATAGTAGGAATTCCAGGGAAGAAATGTGGTACAATCATACTTACAGCAGAGGA
ATTAAACTGTTGCAGGGATGCCGTTTTGATGCAATTTTGTGCGAACAAATTGGACAAGAA
GGACTTCTTTGGAAAATCAGATCCTTTCCTTGTATTTTATCGAAGTAATGAAGATGGCAG
TTTTACAATTTGTCACAAGACAGAAGTTGTCAAAAACACTCTAAATCCAGTATGGCAAGC
ATTCAAGATCTCAGTCAGAGCATTATGTAATGGAGACTATGACAGAACAATCAAAGTAGA
GGTGTATGACTGGGACCGAGATGGAAGTCATGATTTCATTGGAGAATTTACAACAAGCTA
TAGGGAACTTTCTAGAGGGCAGTCACAATTCAACGTATATGAGGTGGTGAATCCCAAAAA
GAAAGGAAAAAAGAAAAAATATACTAATTCTGGAACAGTAACTTTACTCTCTTTCTTGGT
AGAAACAGAAGTTTCATTCCTTGACTACATTAAGGGAGGGACGCAAATCAATTTCACAGT
GGCTATTGATTTTACAGCATCAAACGGCAACCCTGCTCAGCCCACTTCCCCCCACTATAT
GAATCCTTACCAACTGAATGCCTATGGTATGGCACTAAAAGCAGTGGGAGAAATTGTTCA
AGATTATGACAGTGATAAAATGTTTCCAGCTCTAGGATTTGGTGCAAAACTGCCTCCAGA
TGGAAGGATATCTCACGAATTTGCTTTGAATGGGAATCCTCAAAACCCCTACTGTGATGG
CATTGAGGGGGTCATGGAGGCTTATTACAGGAGTCTGAAATCTGTACAACTATATGGGCC
CACCAACTTTGCTCCTGTAATTAATCATGTAGCAAGATATGCTTCTTCTGTAAAGGATGG
CTCCCAGTATTTTGTGCTTCTGATTGTTACAGATGGTGTTATCTCAGATATGGCCCAGAC
TAAGGAGTCCATAGTTAATGCCTCAAAACTTCCAATGTCAATAATTATAGTAGGTGTTGG
ACCAGCAGAATTTGATGCAATGGTCGAATTGGATGGAGATGATGTAAGAGTCTCCTCTAG
AGGAAAATATGCTGAAAGAGACATTGTGCAGTTTGTGCCATTCAGGGATTATATTGACAG
AAGTGGAAACCACATACTGAGCATGGCTAGATTGGCTAAAGATGTCCTAGCTGAGATCCC
TGAGCAGTTTCTCTCCTATATGAGAGCCCGAGGAATCAAGCCATCACCTGCGCCTCCCCC
ATACACCCCACCTACACATGTGTTACAGACTCAAATAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT