NCLN ORF clone pFN21AE0030

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0030

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Clone info.

Species

human

Clone Name

pFN21AE0030 Sequence (with vector) Restriction Map

Product ID

FHC06117

Flexi Clone PID

FXC06117

ORF Sequence

DNA sequence (1656 bp)
Protein sequence (552 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527192]

Gene

KIEE0030

Symbol

NCLN

Alias : NET59

Description

nicalin

Original Clone ID

oc00730

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1656 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 552 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32869.1 0 100.0 NCLN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007484 207 372 PF04389 Peptidase M28
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 ASCLPLGFIVFLPAVLLLVAPPL 26 PRIMARY 23 2 513 FDLLLAVGIAAYLGMAYVAVQHF 535 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_064555 563 609156 100.0 100.0 NP_001308392 562 609156 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0030 Download

>KIEE0030  1656 bp
ATGCTGAAGGCGTCTTGTCTGCCTCTCGGCTTCATCGTCTTCCTGCCCGCTGTGCTGCTG
CTGGTGGCGCCGCCGCTGCCTGCCGCCGACGCCGCGCACGAGTTCACCGTGTACCGCATG
CAGCAGTACGACCTGCAGGGCCAGCCCTACGGCACACGGAATGCAGTGCTGAACACGGAG
GCGCGCACGATGGCGGCGGAGGTGCTGAGCCGCCGCTGCGTGCTCATGCGGCTACTGGAC
TTCTCCTACGAGCAGTACCAGAAGGCCCTGCGGCAGTCGGCGGGCGCCGTGGTCATCATC
CTGCCCAGGGCCATGGCCGCCGTGCCCCAGGACGTCGTCCGGCAATTCATGGAGATCGAG
CCGGAGATGCTGGCCATGGAGACCGCCGTCCCCGTGTACTTTGCCGTGGAGGACGAGGCC
CTGCTGTCTATCTACAAGCAGACCCAGGCTGCCTCCGCCTCCCAGGGCTCCGCCTCTGCT
GCTGAAGTACTGCTGCGCACGGCCACTGCCAACGGCTTCCAGATGGTCACCAGCGGGGTA
CAGAGCAAGGCCGTGAGTGACTGGCTGATTGCCAGCGTGGAGGGGCGGCTGACGGGGCTG
GGCGGAGAGGACCTTCCCACCATCGTCATCGTGGCCCACTACGACGCCTTTGGAGTGGCC
CCCTGGCTGTCGCTGGGCGCGGACTCCAACGGGAGCGGCGTCTCTGTGCTGCTGGAGCTG
GCACGCCTCTTCTCCCGGCTCTACACCTACAAGCGCACGCACGCCGCCTACAACCTCCTG
TTCTTTGCGTCTGGAGGAGGCAAGTTTAACTACCAGGGAACCAAGCGCTGGCTGGAAGAC
AACCTGGACCACACAGACTCCAGCCTGCTTCAGGACAATGTGGCCTTCGTGCTGTGCCTG
GACACCGTGGGCCGGGGCAGCAGCCTGCACCTGCACGTGTCCAAGCCGCCTCGGGAGGGC
ACCCTGCAGCACGCCTTCCTGCGGGAGCTGGAGACGGTGGCCGCGCACCAGTTCCCTGAG
GTACGGTTCTCCATGGTGCACAAGCGGATCAACCTGGCGGAGGACGTGCTGGCCTGGGAG
CACGAGCGCTTCGCCATCCGCCGACTGCCCGCCTTCACGCTGTCCCACCTGGAGAGCCAC
CGTGACGGTCAGCGCAGCAGCATCATGGACGTGCGGTCCCGGGTGGATTCTAAGACCCTG
ACCCGTAACACGAGGATCATTGCAGAGGCCCTGACTCGAGTCATCTACAACCTGACAGAG
AAGGGGACACCCCCAGACATGCCGGTGTTCACAGAGCAGATGCAGATCCAGCAGGAGCAG
CTGGACTCGGTGATGGACTGGCTCACCAACCAGCCGCGGGCCGCGCAGCTGGTGGACAAG
GACAGCACCTTCCTCAGCACGCTGGAGCACCACCTGAGCCGCTACCTGAAGGACGTGAAG
CAGCACCACGTCAAGGCTGACAAGCGGGACCCAGAGTTTGTCTTCTACGACCAGCTGAAG
CAAGTGATGAATGCGTACAGAGTCAAGCCGGCCGTCTTTGACCTGCTCCTGGCTGTTGGC
ATTGCTGCCTACCTCGGCATGGCCTACGTGGCTGTCCAGCACTTCAGCCTCCTCTACAAG
ACCGTCCAGAGGCTGCTCGTGAAGGCCAAGACACAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0030 Download

>KIEE0030  552 aa
MLKASCLPLGFIVFLPAVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTE
ARTMAAEVLSRRCVLMRLLDFSYEQYQKALRQSAGAVVIILPRAMAAVPQDVVRQFMEIE
PEMLAMETAVPVYFAVEDEALLSIYKQTQAASASQGSASAAEVLLRTATANGFQMVTSGV
QSKAVSDWLIASVEGRLTGLGGEDLPTIVIVAHYDAFGVAPWLSLGADSNGSGVSVLLEL
ARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDNLDHTDSSLLQDNVAFVLCL
DTVGRGSSLHLHVSKPPREGTLQHAFLRELETVAAHQFPEVRFSMVHKRINLAEDVLAWE
HERFAIRRLPAFTLSHLESHRDGQRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIYNLTE
KGTPPDMPVFTEQMQIQQEQLDSVMDWLTNQPRAAQLVDKDSTFLSTLEHHLSRYLKDVK
QHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVGIAAYLGMAYVAVQHFSLLYK
TVQRLLVKAKTQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0030 Download

>pFN21AE0030  6361 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGAAGGCGTCTTGTCTGCCTCTCGGCTTCAT
CGTCTTCCTGCCCGCTGTGCTGCTGCTGGTGGCGCCGCCGCTGCCTGCCGCCGACGCCGC
GCACGAGTTCACCGTGTACCGCATGCAGCAGTACGACCTGCAGGGCCAGCCCTACGGCAC
ACGGAATGCAGTGCTGAACACGGAGGCGCGCACGATGGCGGCGGAGGTGCTGAGCCGCCG
CTGCGTGCTCATGCGGCTACTGGACTTCTCCTACGAGCAGTACCAGAAGGCCCTGCGGCA
GTCGGCGGGCGCCGTGGTCATCATCCTGCCCAGGGCCATGGCCGCCGTGCCCCAGGACGT
CGTCCGGCAATTCATGGAGATCGAGCCGGAGATGCTGGCCATGGAGACCGCCGTCCCCGT
GTACTTTGCCGTGGAGGACGAGGCCCTGCTGTCTATCTACAAGCAGACCCAGGCTGCCTC
CGCCTCCCAGGGCTCCGCCTCTGCTGCTGAAGTACTGCTGCGCACGGCCACTGCCAACGG
CTTCCAGATGGTCACCAGCGGGGTACAGAGCAAGGCCGTGAGTGACTGGCTGATTGCCAG
CGTGGAGGGGCGGCTGACGGGGCTGGGCGGAGAGGACCTTCCCACCATCGTCATCGTGGC
CCACTACGACGCCTTTGGAGTGGCCCCCTGGCTGTCGCTGGGCGCGGACTCCAACGGGAG
CGGCGTCTCTGTGCTGCTGGAGCTGGCACGCCTCTTCTCCCGGCTCTACACCTACAAGCG
CACGCACGCCGCCTACAACCTCCTGTTCTTTGCGTCTGGAGGAGGCAAGTTTAACTACCA
GGGAACCAAGCGCTGGCTGGAAGACAACCTGGACCACACAGACTCCAGCCTGCTTCAGGA
CAATGTGGCCTTCGTGCTGTGCCTGGACACCGTGGGCCGGGGCAGCAGCCTGCACCTGCA
CGTGTCCAAGCCGCCTCGGGAGGGCACCCTGCAGCACGCCTTCCTGCGGGAGCTGGAGAC
GGTGGCCGCGCACCAGTTCCCTGAGGTACGGTTCTCCATGGTGCACAAGCGGATCAACCT
GGCGGAGGACGTGCTGGCCTGGGAGCACGAGCGCTTCGCCATCCGCCGACTGCCCGCCTT
CACGCTGTCCCACCTGGAGAGCCACCGTGACGGTCAGCGCAGCAGCATCATGGACGTGCG
GTCCCGGGTGGATTCTAAGACCCTGACCCGTAACACGAGGATCATTGCAGAGGCCCTGAC
TCGAGTCATCTACAACCTGACAGAGAAGGGGACACCCCCAGACATGCCGGTGTTCACAGA
GCAGATGCAGATCCAGCAGGAGCAGCTGGACTCGGTGATGGACTGGCTCACCAACCAGCC
GCGGGCCGCGCAGCTGGTGGACAAGGACAGCACCTTCCTCAGCACGCTGGAGCACCACCT
GAGCCGCTACCTGAAGGACGTGAAGCAGCACCACGTCAAGGCTGACAAGCGGGACCCAGA
GTTTGTCTTCTACGACCAGCTGAAGCAAGTGATGAATGCGTACAGAGTCAAGCCGGCCGT
CTTTGACCTGCTCCTGGCTGTTGGCATTGCTGCCTACCTCGGCATGGCCTACGTGGCTGT
CCAGCACTTCAGCCTCCTCTACAAGACCGTCCAGAGGCTGCTCGTGAAGGCCAAGACACA
GGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T