POU1F1 ORF clone pFN21AB9698

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9698

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Clone info.

Species

human

Clone Name

pFN21AB9698 Sequence (with vector) Restriction Map

Product ID

FHC11948

Flexi Clone PID

FXC11948

ORF Sequence

DNA sequence (873 bp)
Protein sequence (291 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587506]

Gene

KIBB9698

Symbol

POU1F1

Alias : CPHD1, GHF-1, PIT1, POU1F1a, Pit-1

Description

POU class 1 homeobox 1, transcript variant alpha

Original Clone ID

cp00281

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 873 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 291 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2919.1 1.1e-93 100.0 POU1F1 CCDS46873.1 1.6e-79 91.8 POU1F1 CCDS5040.1 1.7e-26 47.0 POU3F2 CCDS33265.1 6.9e-26 50.0 POU3F3 CCDS14450.1 8e-26 43.9 POU3F4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR013847 143 160 PR00028 POU IPR013847 166 179 PR00028 POU IPR013847 182 197 PR00028 POU IPR013847 213 233 PR00028 POU IPR013847 248 263 PR00028 POU HMMPfam IPR000327 125 198 PF00157 POU-specific IPR001356 215 271 PF00046 Homeobox HMMSmart IPR000327 124 198 SM00352 POU-specific IPR001356 214 276 SM00389 Homeobox ProfileScan IPR000327 124 198 PS51179 POU-specific IPR001356 212 272 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000297 291 173110,613038 100.0 100.0 NP_001116229 317 173110,613038 91.8 100.0 NP_005595 443 600494 47.0 83.5 NP_000298 361 300039,304400 43.9 87.6 XP_016872976 356 607394 40.0 83.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9698 Download

>KIBB9698  873 bp
ATGAGTTGCCAAGCTTTTACTTCGGCTGATACCTTTATACCTCTGAATTCTGACGCCTCT
GCAACTCTGCCTCTGATAATGCATCACAGTGCTGCCGAGTGTCTACCAGTCTCCAACCAT
GCCACCAATGTGATGTCTACAGCAACAGGACTTCATTATTCTGTTCCTTCCTGTCATTAT
GGAAACCAGCCATCAACCTATGGAGTGATGGCAGGTAGTTTAACCCCTTGTCTTTATAAA
TTTCCTGACCACACCTTGAGTCATGGATTTCCTCCTATACACCAGCCTCTTCTGGCAGAG
GACCCCACAGCTGCTGATTTCAAGCAGGAACTCAGGCGGAAAAGTAAATTGGTGGAAGAG
CCAATAGACATGGATTCTCCAGAAATCAGAGAACTTGAAAAGTTTGCCAATGAATTTAAA
GTGAGACGAATTAAATTAGGATACACCCAGACAAATGTTGGGGAGGCCCTGGCAGCTGTG
CATGGCTCTGAATTCAGTCAAACAACAATCTGCCGATTTGAAAATCTGCAGCTCAGCTTT
AAAAATGCATGCAAACTGAAAGCAATATTATCCAAATGGCTGGAGGAAGCTGAGCAAGTA
GGAGCTTTGTACAATGAAAAAGTGGGAGCAAATGAAAGGAAAAGAAAACGAAGAACAACT
ATAAGCATTGCTGCTAAAGATGCTCTGGAGAGACACTTTGGAGAACAGAATAAACCTTCT
TCTCAAGAGATCATGAGGATGGCTGAAGAACTGAATCTGGAGAAAGAAGTAGTAAGAGTT
TGGTTTTGCAACCGGAGGCAGAGAGAAAAACGGGTGAAAACAAGTCTGAATCAGAGTTTA
TTTTCTATTTCTAAGGAACATCTTGAGTGCAGA

ORF Protein

Cloned ORF protein sequence for pFN21AB9698 Download

>KIBB9698  291 aa
MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTATGLHYSVPSCHY
GNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEE
PIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSF
KNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPS
SQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9698 Download

>pFN21AB9698  5578 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTTGCCAAGCTTTTACTTCGGCTGATACCTT
TATACCTCTGAATTCTGACGCCTCTGCAACTCTGCCTCTGATAATGCATCACAGTGCTGC
CGAGTGTCTACCAGTCTCCAACCATGCCACCAATGTGATGTCTACAGCAACAGGACTTCA
TTATTCTGTTCCTTCCTGTCATTATGGAAACCAGCCATCAACCTATGGAGTGATGGCAGG
TAGTTTAACCCCTTGTCTTTATAAATTTCCTGACCACACCTTGAGTCATGGATTTCCTCC
TATACACCAGCCTCTTCTGGCAGAGGACCCCACAGCTGCTGATTTCAAGCAGGAACTCAG
GCGGAAAAGTAAATTGGTGGAAGAGCCAATAGACATGGATTCTCCAGAAATCAGAGAACT
TGAAAAGTTTGCCAATGAATTTAAAGTGAGACGAATTAAATTAGGATACACCCAGACAAA
TGTTGGGGAGGCCCTGGCAGCTGTGCATGGCTCTGAATTCAGTCAAACAACAATCTGCCG
ATTTGAAAATCTGCAGCTCAGCTTTAAAAATGCATGCAAACTGAAAGCAATATTATCCAA
ATGGCTGGAGGAAGCTGAGCAAGTAGGAGCTTTGTACAATGAAAAAGTGGGAGCAAATGA
AAGGAAAAGAAAACGAAGAACAACTATAAGCATTGCTGCTAAAGATGCTCTGGAGAGACA
CTTTGGAGAACAGAATAAACCTTCTTCTCAAGAGATCATGAGGATGGCTGAAGAACTGAA
TCTGGAGAAAGAAGTAGTAAGAGTTTGGTTTTGCAACCGGAGGCAGAGAGAAAAACGGGT
GAAAACAAGTCTGAATCAGAGTTTATTTTCTATTTCTAAGGAACATCTTGAGTGCAGAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT