KLHL25 ORF clone pFN21AB9611

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Halotag ORF Clone pFN21AB9611

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Clone info.

Species

human

Clone Name

pFN21AB9611 Sequence (with vector) Restriction Map

Product ID

FHC11732

Flexi Clone PID

FXC11732

ORF Sequence

DNA sequence (1767 bp)
Protein sequence (589 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529312]

Gene

KIBB9611

Symbol

KLHL25

Alias : ENC-2, ENC2

Description

kelch-like family member 25

Original Clone ID

cp00194

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1767 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 589 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10339.1 0 100.0 KLHL25 CCDS4021.1 9.4e-188 74.7 ENC1 CCDS58958.1 5.9e-166 75.2 ENC1 CCDS1310.1 1.4e-55 34.7 KLHL20 CCDS43766.1 2.3e-54 32.4 KLHL38 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 36 143 PF00651 BTB/POZ IPR011705 149 250 PF07707 BTB/Kelch-associated IPR006652 329 374 PF01344 Kelch repeat type 1 IPR006652 377 430 PF01344 Kelch repeat type 1 IPR006652 435 477 PF01344 Kelch repeat type 1 IPR006652 483 524 PF01344 Kelch repeat type 1 IPR006652 528 568 PF01344 Kelch repeat type 1 HMMSmart IPR000210 46 144 SM00225 BTB/POZ-like IPR011705 149 251 SM00875 BTB/Kelch-associated IPR006652 296 340 SM00612 Kelch repeat type 1 IPR006652 341 388 SM00612 Kelch repeat type 1 IPR006652 389 444 SM00612 Kelch repeat type 1 IPR006652 445 492 SM00612 Kelch repeat type 1 IPR006652 493 538 SM00612 Kelch repeat type 1 IPR006652 539 585 SM00612 Kelch repeat type 1 ProfileScan IPR000210 46 114 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001243504 589 605173 74.7 100.0 XP_011541998 589 605173 74.7 100.0 XP_011541999 589 605173 74.7 100.0 NP_003624 589 605173 74.7 100.0 NP_001243503 589 605173 74.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9611 Download

>KIBB9611  1767 bp
ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTC
ACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGC
AAGCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCAC
CGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGG
GAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTG
CTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTG
TTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG
GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAG
TGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGG
CAGAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGAT
GAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGAGGCCATCCTCCAGTGGGTGAAGCAC
GACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCCGCAGCGTGCGTCTGGCCTTG
CTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCATGGCAGACGAG
CGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT
GGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAAGGCGGGCCACACGCTACTCATCCTG
GGGGGCCAGACCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATC
ATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGC
AAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAACGGGGTCTCCAAGGATGTCTGGGTG
TACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCATGCTGATTGCCCGCTTTGGC
CATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACACATCCCTGGCA
GGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG
GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTG
AGTGCCAAGCTGAAGCTCTTTGTTTTTGGAGGAACCAGCATCCACCGGGACATGGTGTCC
AAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAG
CCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGAC
ACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTGAGACCAACCAGTGGACGCGG
ATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGGCAACAAGCTC
TATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC
ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTT
GTCAGCACCTGGAAGCACCTGCCCGCG

ORF Protein

Cloned ORF protein sequence for pFN21AB9611 Download

>KIBB9611  589 aa
MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCH
RAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIAINEENAESL
LEAGDMLQFHDVRDAAAEFLEKNLFPSNCLGMMLLSDAHQCRRLYEFSWRMCLVHFETVR
QSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLAL
LPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCARPRKAGHTLLIL
GGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWV
YDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGVFPASPSVSLKQVEKYDPG
ANKWMMVAPLRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCYDPSENRWTIKAECPQ
PWRYTAAAVLGSQIFIMGGDTEFTAASAYRFDCETNQWTRIGDMTAKRMSCHALASGNKL
YVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9611 Download

>pFN21AB9611  6472 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCG
GAGCAGCACGGGGTCCATGAACGTCACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGT
GCTGGCCCACCTCAACACGCTTCGCAAGCACTGCATGTTCACCGACGTCACACTCTGGGC
GGGCGACCGTGCCTTCCCCTGTCACCGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGA
GGCCATGTTCAGCCATGGCCTTCGGGAGAGCCGGGATGACACTGTCAACTTCCAGGACAA
CCTGCACCCGGAGGTGCTGGAGCTGCTGCTGGACTTTGCCTACTCCTCACGCATCGCCAT
CAACGAGGAGAACGCTGAGTCACTGTTGGAGGCAGGCGACATGCTGCAGTTCCACGATGT
GCGGGATGCTGCCGCCGAGTTCCTGGAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCAT
GATGCTGCTCTCGGACGCCCACCAGTGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTG
CCTGGTGCACTTTGAGACGGTGAGGCAGAGCGAGGACTTCAACAGCCTGTCCAAGGACAC
ACTGCTGGACCTCATCTCGAGTGATGAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGA
GGCCATCCTCCAGTGGGTGAAGCACGACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCT
CCTCCGCAGCGTGCGTCTGGCCTTGCTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAG
CGAGGCCCTCCTCATGGCAGACGAGCGCACCAAGCTTATCATGGATGAGGCCCTGCGCTG
CAAGACCAGGATCCTGCAGAATGATGGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAA
GGCGGGCCACACGCTACTCATCCTGGGGGGCCAGACCTTCATGTGTGACAAGATCTACCA
GGTGGACCACAAGGCCAAGGAGATCATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGA
GTTCAGCGCCTCAGCGATCGGCTGCAAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAA
CGGGGTCTCCAAGGATGTCTGGGTGTACGACACCGTACATGAGGAATGGTCCAAGGCGGC
GCCCATGCTGATTGCCCGCTTTGGCCATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGT
GGTGGGGGGACACACATCCCTGGCAGGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAA
ACAAGTGGAGAAATACGACCCTGGGGCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGA
TGGCGTCAGCAATGCCGCAGTGGTGAGTGCCAAGCTGAAGCTCTTTGTTTTTGGAGGAAC
CAGCATCCACCGGGACATGGTGTCCAAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTG
GACGATCAAGGCCGAGTGCCCCCAGCCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAG
CCAGATCTTCATCATGGGAGGTGACACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGA
CTGTGAGACCAACCAGTGGACGCGGATTGGGGACATGACTGCCAAGCGCATGTCCTGCCA
TGCCCTGGCTTCCGGCAACAAGCTCTATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTG
TAAGACTCTGGACTGCTATGACCCCACTTCAGATACATGGAACTGCATCACCACAGTGCC
CTACTCACTTATCCCCACGGCCTTTGTCAGCACCTGGAAGCACCTGCCCGCGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT