ANAPC1 ORF clone pFN21AB9351

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9351

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Clone info.

Species

human

Clone Name

pFN21AB9351 Sequence (with vector) Restriction Map

Product ID

FHC01858

Flexi Clone PID

FXC01858

Original Clone PID

ORK01858

ORF Sequence

DNA sequence (5832 bp)
Protein sequence (1944 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385205]

Gene

KIBB9351

Symbol

ANAPC1

Alias : APC1, MCPR, TSG24

Description

anaphase promoting complex subunit 1

Original Clone ID

ha02361

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5832 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1944 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2093.1 0 100.0 ANAPC1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition None - - - - -
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 752 STLLFTHIPAIFFVLHLVYEELK 774 PRIMARY 23 2 776 NTLMGEGICSLVELLVQLARDLK 798 SECONDARY 23 3 871 RLVVLSIALYILGDESLVSDESS 893 SECONDARY 23 4 1457 LSQAHVYIIAGACLSLGFRFAGS 1479 SECONDARY 23 5 1510 HNLETCLSVVLLSLAMVMAGSGN 1532 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_073153 1944 608473 100.0 100.0 XP_011509935 1948 608473 99.8 100.0 XP_016860200 1917 608473 100.0 98.1 XP_016860199 1921 608473 99.8 98.1 XP_016860201 1846 608473 100.0 95.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9351 Download

>KIBB9351  5832 bp
ATGTCGAACTTCTATGAAGAAAGGACAACGATGATTGCAGCAAGGGATTTGCAGGAATTT
GTTCCTTTTGGTCGAGACCACTGCAAGCACCACCCTAATGCTTTGAACCTTCAACTTCGC
CAGCTGCAGCCAGCTTCTGAATTATGGTCTTCTGATGGTGCTGCTGGCTTGGTGGGATCC
CTTCAGGAGGTTACAATCCACGAGAAACAGAAGGAAAGCTGGCAGTTAAGGAAAGGAGTA
AGTGAAATTGGAGAAGATGTGGACTATGATGAGGAACTCTATGTTGCTGGAAATATGGTG
ATATGGAGCAAAGGAAGTAAAAGCCAGGCATTGGCAGTTTATAAAGCATTTACAGTTGAC
AGTCCTGTTCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG
GCCTACAGTAGCAATGAAGTAGAAAAATGCATATGTATATTGCAAAGCTCATGTATTAAC
ATGCATAGCATAGAAGGAAAGGATTACATAGCTTCATTACCATTTCAGGTTGCAAATGTT
TGGCCCACTAAATATGGATTGCTGTTTGAACGAAGCGCTTCTTCACATGAAGTACCTCCA
GGTTCACCCAGAGAACCTTTACCTACTATGTTCAGCATGCTGCACCCACTAGATGAAATA
ACTCCACTTGTTTGTAAATCTGGAAGTCTTTTTGGTTCATCACGGGTGCAATATGTTGTA
GATCATGCAATGAAAATTGTTTTCCTCAATACTGACCCCTCTATTGTAATGACTTATGAT
GCTGTTCAAAATGTGCATTCTGTGTGGACTCTCCGGAGAGTCAAATCAGAGGAAGAGAAT
GTTGTTTTAAAGTTCTCTGAACAGGGGGGAACCCCACAGAATGTGGCCACTAGCAGCTCC
CTCACAGCACATCTCAGAAGCCTCTCCAAAGGAGATTCCCCTGTGACTTCACCTTTCCAG
AATTACTCCTCCATTCACAGCCAGAGTCGCTCAACCTCATCACCCAGTCTACATTCTCGC
TCACCTTCTATTTCCAACATGGCAGCTCTAAGTCGTGCTCATTCTCCTGCGTTAGGAGTG
CACTCTTTTTCAGGGGTGCAAAGGTTCAACATTTCAAGCCATAATCAGTCTCCAAAGAGA
CATAGTATTTCTCATTCTCCAAATAGTAATTCTAATGGCTCCTTTCTTGCACCAGAAACG
GAGCCAATTGTTCCTGAACTGTGTATTGACCATTTGTGGACAGAAACGATTACTAATATA
AGAGAGAAAAATTCACAAGCCTCAAAAGTGTTTATTACATCTGACCTATGTGGGCAAAAG
TTCCTGTGCTTTTTAGTAGAGTCCCAGCTCCAGTTACGCTGTGTAAAGTTTCAAGAGAGT
AATGATAAAACCCAGCTCATCTTTGGTTCAGTGACCAACATACCAGCAAAGGATGCAGCA
CCAGTGGAGAAAATAGACACCATGCTGGTCTTGGAAGGCAGTGGAAACCTGGTGCTATAC
ACAGGAGTGGTTCGGGTGGGAAAGGTTTTTATTCCTGGACTGCCAGCTCCCTCTCTGACG
ATGTCCAACACAATGCCTCGGCCCAGTACTCCACTAGATGGCGTTAGTACTCCAAAGCCT
CTTAGTAAACTCCTTGGATCATTGGACGAGGTTGTTCTGTTGTCCCCAGTTCCAGAACTG
AGGGATTCTTCAAAACTTCATGATTCTCTCTATAATGAGGATTGTACTTTCCAACAGCTT
GGAACTTACATTCATTCTATCAGAGATCCTGTCCATAACAGAGTCACCCTAGAACTGAGT
AATGGCTCCATGGTTAGGATCACTATTCCTGAAATTGCCACCTCTGAGTTAGTACAAACG
TGTTTGCAAGCAATTAAGTTTATCCTGCCAAAAGAAATAGCAGTTCAGATGCTTGTCAAG
TGGTACAATGTCCACAGTGCTCCAGGAGGACCCAGTTATCACTCAGAGTGGAATTTATTT
GTGACTTGTCTCATGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACTAGAAAT
TTTGACTTTGAAGGATCACTTTCTCCTGTCATTGCGCCCAAAAAAGCAAGGCCTTCCGAG
ACTGGATCTGATGATGACTGGGAATATTTACTAAATTCAGACTACCACCAGAATGTTGAG
TCTCATCTTTTGAACAGATCTTTATGTCTGAGTCCTTCAGAAGCTTCACAGATGAAGGAT
GAGGATTTTTCACAGAATCTCAGTCTGGATTCTTCTACACTTCTCTTTACTCACATACCT
GCAATTTTTTTCGTTCTTCACCTTGTGTATGAGGAGCTTAAGTTGAATACTCTAATGGGA
GAAGGAATTTGTTCACTTGTTGAACTTCTCGTTCAGTTGGCAAGGGACTTAAAATTGGGG
CCTTATGTAGATCATTACTATAGAGACTACCCAACGCTTGTCAGAACTACTGGACAAGTG
TGCACAATTGATCCAGGTCAAACAGGATTTATGCATCATCCATCATTTTTTACGTCTGAG
CCACCAAGTATTTATCAGTGGGTGAGTTCTTGTCTGAAGGGTGAAGGAATGCCACCTTAT
CCTTACCTCCCTGGAATCTGTGAAAGAAGCAGACTTGTAGTCTTGAGTATTGCACTGTAC
ATACTTGGTGATGAGAGCTTGGTTTCTGATGAATCCTCACAGTATTTAACCAGAATAACT
ATAGCCCCCCAGAAGTTGCAAGTAGAACAAGAGGAAAACAGGTTTAGTTTCAGGCATTCT
ACATCTGTTTCTAGTCTAGCTGAAAGATTGGTTGTCTGGATGACTAATGTAGGATTCACT
TTAAGAGATTTGGAAACTCTTCCCTTTGGAATTGCTCTTCCCATCAGAGATGCAATTTAT
CACTGTCGTGAGCAGCCTGCCTCAGACTGGCCAGAAGCTGTCTGTCTCTTGATTGGACGT
CAGGATCTTTCCAAGCAGGCCTGCGAAGGAAACTTACCCAAAGGGAAGTCTGTGCTCTCA
TCAGATGTTCCTTCAGGAACAGAAACTGAGGAGGAAGATGACGGCATGAATGACATGAAT
CACGAGGTCATGTCATTAATATGGAGTGAAGATTTAAGGGTGCAGGATGTGCGAAGGCTT
CTTCAGAGTGCGCATCCTGTCCGTGTCAACGTAGTGCAGTACCCAGAGCTCAGTGACCAC
GAGTTCATCGAGGAAAAGGAAAACAGATTGCTCCAATTGTGTCAGCGAACTATGGCTCTT
CCTGTAGGACGAGGAATGTTTACCTTGTTTTCGTACCATCCTGTTCCAACAGAGCCATTG
CCTATTCCTAAATTGAATCTGACTGGGCGTGCCCCTCCTCGGAACACAACAGTAGACCTT
AATAGTGGAAACATCGATGTGCCTCCCAACATGACAAGCTGGGCCAGCTTTCATAATGGT
GTGGCTGCTGGCCTGAAGATAGCGCCTGCCTCCCAGATCGACTCAGCTTGGATTGTTTAC
AATAAGCCCAAGCATGCTGAGTTGGCCAATGAGTATGCTGGCTTTCTCATGGCTCTGGGT
TTGAATGGGCACCTTACCAAGCTGGCGACTCTCAATATCCATGACTACTTGACCAAGGGC
CATGAAATGACAAGCATTGGACTGCTACTTGGTGTTTCTGCTGCAAAACTAGGCACCATG
GATATGTCTATTACTCGGCTTCTTAGCATTCACATTCCTGCTCTCTTACCCCCAACGTCC
ACAGAGCTGGATGTTCCTCACAATGTCCAAGTGGCTGCAGTGGTTGGCATTGGCCTTGTA
TATCAAGGGACAGCTCACAGACATACTGCAGAAGTCCTGTTGGCTGAGATAGGACGGCCT
CCTGGTCCTGAAATGGAATACTGCACTGACAGAGAGTCATACTCCTTAGCTGCTGGCTTG
GCCCTGGGCATGGTCTGCTTGGGGCATGGCAGCAATTTGATAGGTATGTCTGATCTCAAT
GTGCCTGAGCAGCTCTATCAGTACATGGTTGGAGGACATAGGCGCTTTCAAACAGGAATG
CATAGGGAGAAACATAAATCACCAAGTTATCAAATCAAAGAAGGAGATACCATAAATGTG
GATGTGACTTGTCCAGGTGCTACTCTAGCTTTGGCTATGATCTACTTAAAAACCAATAAC
AGATCTATTGCAGATTGGCTCCGAGCCCCTGACACCATGTATTTGTTGGACTTTGTGAAG
CCAGAATTTCTCTTGCTTAGGACACTTGCTCGATGCCTGATTTTGTGGGATGATATTTTA
CCAAATTCCAAGTGGGTTGACAGCAATGTTCCTCAAATTATAAGAGAAAATAGTATCTCT
CTCAGTGAAATCGAATTGCCGTGCTCAGAGGATTTGAATTTGGAAACTTTGTCCCAAGCA
CATGTCTACATAATTGCAGGAGCCTGCTTGTCTCTGGGTTTTCGATTTGCTGGCTCAGAA
AACTTATCAGCATTTAACTGTTTGCATAAATTTGCCAAAGATTTTATGACTTATTTGTCT
GCACCTAATGCTTCTGTTACAGGTCCTCATAACCTAGAAACTTGTCTGAGCGTGGTGCTG
CTGTCTCTCGCCATGGTCATGGCTGGCTCAGGAAACCTAAAGGTTTTGCAGCTTTGTCGC
TTCTTACACATGAAAACGGGTGGTGAAATGAACTATGGTTTTCACTTAGCCCACCACATG
GCCCTTGGACTTCTATTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCAAATTCTTCC
ATTGCCGCTCTTCTCTGTGCCCTTTATCCGCACTTCCCAGCTCACAGCACTGACAACCGG
TATCATCTCCAGGCTCTCCGGCACCTCTATGTGCTGGCCGCGGAGCCCAGGCTTCTAGTG
CCTGTGGATGTGGACACAAACACGCCCTGCTATGCCCTCTTAGAAGTTACCTACAAGGGC
ACTCAGTGGTATGAACAAACCAAAGAAGAATTGATGGCTCCTACCCTTCTTCCAGAACTC
CATCTTTTAAAGCAGATTAAAGTAAAAGGCCCAAGATACTGGGAACTGCTCATAGATTTA
AGCAAAGGAACACAACACTTGAAGTCCATCCTTTCCAAGGATGGGGTTTTATATGTTAAA
CTCCGGGCGGGTCAGCTCTCCTACAAAGAAGATCCAATGGGATGGCAAAGTTTGTTGGCT
CAGACTGTTGCTAACAGGAACTCTGAAGCCCGGGCTTTCAAGCCAGAAACAATCTCAGCA
TTCACTTCTGATCCAGCACTTCTGTCATTTGCTGAATATTTCTGCAAGCCAACTGTGAAC
ATGGGTCAGAAACAGGAAATTCTGGATCTCTTTTCTTCAGTACTCTATGAATGTGTTACC
CAGGAGACCCCAGAGATGTTGCCTGCATACATAGCAATGGATCAGGCTATAAGAAGACTT
GGGAGAAGAGAAATGTCTGAGACTTCTGAACTTTGGCAGATAAAGTTGGTGTTAGAGTTT
TTCAGCTCCCGAAGCCATCAGGAGCGGCTGCAGAACCACCCTAAGCGGGGGCTCTTTATG
AACTCGGAATTCCTCCCTGTTGTGAAGTGCACCATTGATAATACCCTGGACCAGTGGCTA
CAAGTCGGGGGTGATATGTGTGTGCACGCCTACCTCAGCGGGCAGCCCTTGGAGGAATCA
CAGCTGAGCATGCTGGCCTGCTTCCTCGTCTACCACTCTGTGCCAGCTCCACAGCACCTG
CCACCTATAGGACTAGAAGGGAGCACAAGCTTTGCTGAACTGCTCTTCAAATTTAAGCAG
CTAAAAATGCCAGTGCGAGCTTTGCTGAGATTGGCTCCTTTGCTTCTTGGAAATCCACAG
CCAATGGTGATG

ORF Protein

Cloned ORF protein sequence for pFN21AB9351 Download

>KIBB9351  1944 aa
MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGS
LQEVTIHEKQKESWQLRKGVSEIGEDVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVD
SPVQQALWCDFIISQDKSEKAYSSNEVEKCICILQSSCINMHSIEGKDYIASLPFQVANV
WPTKYGLLFERSASSHEVPPGSPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVV
DHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKSEEENVVLKFSEQGGTPQNVATSSS
LTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGV
HSFSGVQRFNISSHNQSPKRHSISHSPNSNSNGSFLAPETEPIVPELCIDHLWTETITNI
REKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAKDAA
PVEKIDTMLVLEGSGNLVLYTGVVRVGKVFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKP
LSKLLGSLDEVVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELS
NGSMVRITIPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYNVHSAPGGPSYHSEWNLF
VTCLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDDDWEYLLNSDYHQNVE
SHLLNRSLCLSPSEASQMKDEDFSQNLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMG
EGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSE
PPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRIT
IAPQKLQVEQEENRFSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIY
HCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMN
HEVMSLIWSEDLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMAL
PVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSGNIDVPPNMTSWASFHNG
VAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKG
HEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLV
YQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLN
VPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNN
RSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSIS
LSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLS
APNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHM
ALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLV
PVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDL
SKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISA
FTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRL
GRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWL
QVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQ
LKMPVRALLRLAPLLLGNPQPMVM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9351 Download

>pFN21AB9351  10537 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGAACTTCTATGAAGAAAGGACAACGATGAT
TGCAGCAAGGGATTTGCAGGAATTTGTTCCTTTTGGTCGAGACCACTGCAAGCACCACCC
TAATGCTTTGAACCTTCAACTTCGCCAGCTGCAGCCAGCTTCTGAATTATGGTCTTCTGA
TGGTGCTGCTGGCTTGGTGGGATCCCTTCAGGAGGTTACAATCCACGAGAAACAGAAGGA
AAGCTGGCAGTTAAGGAAAGGAGTAAGTGAAATTGGAGAAGATGTGGACTATGATGAGGA
ACTCTATGTTGCTGGAAATATGGTGATATGGAGCAAAGGAAGTAAAAGCCAGGCATTGGC
AGTTTATAAAGCATTTACAGTTGACAGTCCTGTTCAGCAGGCATTGTGGTGTGACTTCAT
TATATCACAGGATAAGTCTGAAAAGGCCTACAGTAGCAATGAAGTAGAAAAATGCATATG
TATATTGCAAAGCTCATGTATTAACATGCATAGCATAGAAGGAAAGGATTACATAGCTTC
ATTACCATTTCAGGTTGCAAATGTTTGGCCCACTAAATATGGATTGCTGTTTGAACGAAG
CGCTTCTTCACATGAAGTACCTCCAGGTTCACCCAGAGAACCTTTACCTACTATGTTCAG
CATGCTGCACCCACTAGATGAAATAACTCCACTTGTTTGTAAATCTGGAAGTCTTTTTGG
TTCATCACGGGTGCAATATGTTGTAGATCATGCAATGAAAATTGTTTTCCTCAATACTGA
CCCCTCTATTGTAATGACTTATGATGCTGTTCAAAATGTGCATTCTGTGTGGACTCTCCG
GAGAGTCAAATCAGAGGAAGAGAATGTTGTTTTAAAGTTCTCTGAACAGGGGGGAACCCC
ACAGAATGTGGCCACTAGCAGCTCCCTCACAGCACATCTCAGAAGCCTCTCCAAAGGAGA
TTCCCCTGTGACTTCACCTTTCCAGAATTACTCCTCCATTCACAGCCAGAGTCGCTCAAC
CTCATCACCCAGTCTACATTCTCGCTCACCTTCTATTTCCAACATGGCAGCTCTAAGTCG
TGCTCATTCTCCTGCGTTAGGAGTGCACTCTTTTTCAGGGGTGCAAAGGTTCAACATTTC
AAGCCATAATCAGTCTCCAAAGAGACATAGTATTTCTCATTCTCCAAATAGTAATTCTAA
TGGCTCCTTTCTTGCACCAGAAACGGAGCCAATTGTTCCTGAACTGTGTATTGACCATTT
GTGGACAGAAACGATTACTAATATAAGAGAGAAAAATTCACAAGCCTCAAAAGTGTTTAT
TACATCTGACCTATGTGGGCAAAAGTTCCTGTGCTTTTTAGTAGAGTCCCAGCTCCAGTT
ACGCTGTGTAAAGTTTCAAGAGAGTAATGATAAAACCCAGCTCATCTTTGGTTCAGTGAC
CAACATACCAGCAAAGGATGCAGCACCAGTGGAGAAAATAGACACCATGCTGGTCTTGGA
AGGCAGTGGAAACCTGGTGCTATACACAGGAGTGGTTCGGGTGGGAAAGGTTTTTATTCC
TGGACTGCCAGCTCCCTCTCTGACGATGTCCAACACAATGCCTCGGCCCAGTACTCCACT
AGATGGCGTTAGTACTCCAAAGCCTCTTAGTAAACTCCTTGGATCATTGGACGAGGTTGT
TCTGTTGTCCCCAGTTCCAGAACTGAGGGATTCTTCAAAACTTCATGATTCTCTCTATAA
TGAGGATTGTACTTTCCAACAGCTTGGAACTTACATTCATTCTATCAGAGATCCTGTCCA
TAACAGAGTCACCCTAGAACTGAGTAATGGCTCCATGGTTAGGATCACTATTCCTGAAAT
TGCCACCTCTGAGTTAGTACAAACGTGTTTGCAAGCAATTAAGTTTATCCTGCCAAAAGA
AATAGCAGTTCAGATGCTTGTCAAGTGGTACAATGTCCACAGTGCTCCAGGAGGACCCAG
TTATCACTCAGAGTGGAATTTATTTGTGACTTGTCTCATGAACATGATGGGTTATAACAC
AGACCGCTTAGCATGGACTAGAAATTTTGACTTTGAAGGATCACTTTCTCCTGTCATTGC
GCCCAAAAAAGCAAGGCCTTCCGAGACTGGATCTGATGATGACTGGGAATATTTACTAAA
TTCAGACTACCACCAGAATGTTGAGTCTCATCTTTTGAACAGATCTTTATGTCTGAGTCC
TTCAGAAGCTTCACAGATGAAGGATGAGGATTTTTCACAGAATCTCAGTCTGGATTCTTC
TACACTTCTCTTTACTCACATACCTGCAATTTTTTTCGTTCTTCACCTTGTGTATGAGGA
GCTTAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCACTTGTTGAACTTCTCGTTCA
GTTGGCAAGGGACTTAAAATTGGGGCCTTATGTAGATCATTACTATAGAGACTACCCAAC
GCTTGTCAGAACTACTGGACAAGTGTGCACAATTGATCCAGGTCAAACAGGATTTATGCA
TCATCCATCATTTTTTACGTCTGAGCCACCAAGTATTTATCAGTGGGTGAGTTCTTGTCT
GAAGGGTGAAGGAATGCCACCTTATCCTTACCTCCCTGGAATCTGTGAAAGAAGCAGACT
TGTAGTCTTGAGTATTGCACTGTACATACTTGGTGATGAGAGCTTGGTTTCTGATGAATC
CTCACAGTATTTAACCAGAATAACTATAGCCCCCCAGAAGTTGCAAGTAGAACAAGAGGA
AAACAGGTTTAGTTTCAGGCATTCTACATCTGTTTCTAGTCTAGCTGAAAGATTGGTTGT
CTGGATGACTAATGTAGGATTCACTTTAAGAGATTTGGAAACTCTTCCCTTTGGAATTGC
TCTTCCCATCAGAGATGCAATTTATCACTGTCGTGAGCAGCCTGCCTCAGACTGGCCAGA
AGCTGTCTGTCTCTTGATTGGACGTCAGGATCTTTCCAAGCAGGCCTGCGAAGGAAACTT
ACCCAAAGGGAAGTCTGTGCTCTCATCAGATGTTCCTTCAGGAACAGAAACTGAGGAGGA
AGATGACGGCATGAATGACATGAATCACGAGGTCATGTCATTAATATGGAGTGAAGATTT
AAGGGTGCAGGATGTGCGAAGGCTTCTTCAGAGTGCGCATCCTGTCCGTGTCAACGTAGT
GCAGTACCCAGAGCTCAGTGACCACGAGTTCATCGAGGAAAAGGAAAACAGATTGCTCCA
ATTGTGTCAGCGAACTATGGCTCTTCCTGTAGGACGAGGAATGTTTACCTTGTTTTCGTA
CCATCCTGTTCCAACAGAGCCATTGCCTATTCCTAAATTGAATCTGACTGGGCGTGCCCC
TCCTCGGAACACAACAGTAGACCTTAATAGTGGAAACATCGATGTGCCTCCCAACATGAC
AAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCTGGCCTGAAGATAGCGCCTGCCTCCCA
GATCGACTCAGCTTGGATTGTTTACAATAAGCCCAAGCATGCTGAGTTGGCCAATGAGTA
TGCTGGCTTTCTCATGGCTCTGGGTTTGAATGGGCACCTTACCAAGCTGGCGACTCTCAA
TATCCATGACTACTTGACCAAGGGCCATGAAATGACAAGCATTGGACTGCTACTTGGTGT
TTCTGCTGCAAAACTAGGCACCATGGATATGTCTATTACTCGGCTTCTTAGCATTCACAT
TCCTGCTCTCTTACCCCCAACGTCCACAGAGCTGGATGTTCCTCACAATGTCCAAGTGGC
TGCAGTGGTTGGCATTGGCCTTGTATATCAAGGGACAGCTCACAGACATACTGCAGAAGT
CCTGTTGGCTGAGATAGGACGGCCTCCTGGTCCTGAAATGGAATACTGCACTGACAGAGA
GTCATACTCCTTAGCTGCTGGCTTGGCCCTGGGCATGGTCTGCTTGGGGCATGGCAGCAA
TTTGATAGGTATGTCTGATCTCAATGTGCCTGAGCAGCTCTATCAGTACATGGTTGGAGG
ACATAGGCGCTTTCAAACAGGAATGCATAGGGAGAAACATAAATCACCAAGTTATCAAAT
CAAAGAAGGAGATACCATAAATGTGGATGTGACTTGTCCAGGTGCTACTCTAGCTTTGGC
TATGATCTACTTAAAAACCAATAACAGATCTATTGCAGATTGGCTCCGAGCCCCTGACAC
CATGTATTTGTTGGACTTTGTGAAGCCAGAATTTCTCTTGCTTAGGACACTTGCTCGATG
CCTGATTTTGTGGGATGATATTTTACCAAATTCCAAGTGGGTTGACAGCAATGTTCCTCA
AATTATAAGAGAAAATAGTATCTCTCTCAGTGAAATCGAATTGCCGTGCTCAGAGGATTT
GAATTTGGAAACTTTGTCCCAAGCACATGTCTACATAATTGCAGGAGCCTGCTTGTCTCT
GGGTTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAACTGTTTGCATAAATTTGC
CAAAGATTTTATGACTTATTTGTCTGCACCTAATGCTTCTGTTACAGGTCCTCATAACCT
AGAAACTTGTCTGAGCGTGGTGCTGCTGTCTCTCGCCATGGTCATGGCTGGCTCAGGAAA
CCTAAAGGTTTTGCAGCTTTGTCGCTTCTTACACATGAAAACGGGTGGTGAAATGAACTA
TGGTTTTCACTTAGCCCACCACATGGCCCTTGGACTTCTATTTTTGGGAGGAGGAAGGTA
CTCTTTGAGCACATCAAATTCTTCCATTGCCGCTCTTCTCTGTGCCCTTTATCCGCACTT
CCCAGCTCACAGCACTGACAACCGGTATCATCTCCAGGCTCTCCGGCACCTCTATGTGCT
GGCCGCGGAGCCCAGGCTTCTAGTGCCTGTGGATGTGGACACAAACACGCCCTGCTATGC
CCTCTTAGAAGTTACCTACAAGGGCACTCAGTGGTATGAACAAACCAAAGAAGAATTGAT
GGCTCCTACCCTTCTTCCAGAACTCCATCTTTTAAAGCAGATTAAAGTAAAAGGCCCAAG
ATACTGGGAACTGCTCATAGATTTAAGCAAAGGAACACAACACTTGAAGTCCATCCTTTC
CAAGGATGGGGTTTTATATGTTAAACTCCGGGCGGGTCAGCTCTCCTACAAAGAAGATCC
AATGGGATGGCAAAGTTTGTTGGCTCAGACTGTTGCTAACAGGAACTCTGAAGCCCGGGC
TTTCAAGCCAGAAACAATCTCAGCATTCACTTCTGATCCAGCACTTCTGTCATTTGCTGA
ATATTTCTGCAAGCCAACTGTGAACATGGGTCAGAAACAGGAAATTCTGGATCTCTTTTC
TTCAGTACTCTATGAATGTGTTACCCAGGAGACCCCAGAGATGTTGCCTGCATACATAGC
AATGGATCAGGCTATAAGAAGACTTGGGAGAAGAGAAATGTCTGAGACTTCTGAACTTTG
GCAGATAAAGTTGGTGTTAGAGTTTTTCAGCTCCCGAAGCCATCAGGAGCGGCTGCAGAA
CCACCCTAAGCGGGGGCTCTTTATGAACTCGGAATTCCTCCCTGTTGTGAAGTGCACCAT
TGATAATACCCTGGACCAGTGGCTACAAGTCGGGGGTGATATGTGTGTGCACGCCTACCT
CAGCGGGCAGCCCTTGGAGGAATCACAGCTGAGCATGCTGGCCTGCTTCCTCGTCTACCA
CTCTGTGCCAGCTCCACAGCACCTGCCACCTATAGGACTAGAAGGGAGCACAAGCTTTGC
TGAACTGCTCTTCAAATTTAAGCAGCTAAAAATGCCAGTGCGAGCTTTGCTGAGATTGGC
TCCTTTGCTTCTTGGAAATCCACAGCCAATGGTGATGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT