RASGRF1 ORF clone pFN21AB9098

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9098

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Clone info.

Species

human

Clone Name

pFN21AB9098 Sequence (with vector) Restriction Map

Product ID

FHC01848

Flexi Clone PID

FXC01848

Original Clone PID

ORK01848

ORF Sequence

DNA sequence (3771 bp)
Protein sequence (1257 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385192]

Gene

KIBB9098

Symbol

RASGRF1

Alias : CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, ras-GRF1

Description

Ras protein-specific guanine nucleotide-releasing factor 1, transcript variant 3

Original Clone ID

ah00691

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 3771 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1257 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45320.1 0 100.0 RASGRF1 CCDS10309.1 1.6e-197 98.7 RASGRF1 CCDS4052.1 1.8e-183 66.5 RASGRF2 CCDS42065.1 7.6e-155 100.0 RASGRF1 CCDS1802.1 1.3e-20 28.7 SOS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 25 127 PF00169 Pleckstrin homology domain IPR000219 245 423 PF00621 Dbl homology (DH) domain IPR000651 634 966 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 1021 1204 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR001849 23 131 SM00233 Pleckstrin homology domain IPR000219 244 425 SM00325 Dbl homology (DH) domain IPR001849 457 586 SM00233 Pleckstrin homology domain IPR000651 630 766 SM00229 Ras-like guanine nucleotide exchange factor IPR000651 865 992 SM00229 Ras-like guanine nucleotide exchange factor IPR001895 1018 1255 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR001849 22 129 PS50003 Pleckstrin homology domain IPR000048 204 229 PS50096 IQ motif IPR000219 240 426 PS50010 Dbl homology (DH) domain IPR001849 467 584 PS50003 Pleckstrin homology domain IPR000651 631 746 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 1022 1254 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001139120 1257 606600 100.0 100.0 XP_016877945 1260 606600 99.8 100.0 XP_016877947 1245 606600 100.0 92.6 XP_016877948 1185 606600 99.7 92.6 XP_016877946 1248 606600 99.7 92.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9098 Download

>KIBB9098  3771 bp
ATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCCCTGGGACTGCTGGCGCGC
AAGGACGGCACGCGCAAAGGCTACCTGAGCAAGCGGAGTTCGGACAACACAAAATGGCAA
ACCAAGTGGTTCGCGCTGCTGCAGAACCTGCTCTTCTACTTCGAGAGCGACTCGAGCTCG
CGGCCCTCGGGGCTTTACCTGCTGGAGGGCTGCGTCTGCGACCGCGCGCCCTCCCCCAAG
CCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAGCATTACTTCACGGTGAACTTCAGC
CATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGAGGACGCAAAAGATTGTGACGAATGG
GTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACAGAGCATGAGGCATTAATG
CAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAGACCGTGGCCAAGCAGCTT
CGGCAGCAGATCGAGGATGGGGAGATCGAGATCGAGCGGCTGAAGGCAGAGATCACATCC
CTGCTCAAGGACAATGAGCGCATCCAGTCCACCCAGACTGTCGCCCCCAACGATGAAGAC
AGCGACATCAAGAAAATTAAGAAGGTGCAGAGCTTCCTGCGGGGCTGGCTGTGCCGGCGG
AAGTGGAAGACCATCATCCAGGACTACATCCGGTCACCCCATGCTGACAGCATGCGCAAG
AGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGAGGCTGAGTACGTGCAGCAGCTGCAC
ATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGATGGCCGCCAGCTCCAAGAAGCCTCCC
ATCACACACGACGACGTCAGCAGCATCTTCCTGAACAGCGAAACCATCATGTTTTTACAT
CAGATCTTTTACCAAGGCCTGAAGGCCCGCATCTCCAGCTGGCCCACGCTGGTCCTGGCT
GACCTATTTGACATCCTGCTGCCCATGCTCAACATCTACCAAGAGTTCGTCCGCAACCAC
CAGTACAGCCTGCAGATCCTGGCCCACTGCAAGCAGAACCGTGACTTCGACAAGCTGCTG
AAGCACTACGAGGCCAAGCCTGACTGCGAGGAGAGGACGCTGGAGACCTTCCTCACCTAC
CCCATGTTCCAGATCCCCAGGTACATCCTGACCCTCCATGAGCTCCTGGCCCACACGCCT
CATGAGCACGTTGAGCGCAACAGCCTGGACTACGCCAAGTCCAAACTGGAGGAGCTGTCC
AGAATAATGCACGATGAAGTAAGTGAGACGGAGAACATCCGGAAAAACCTGGCCATCGAG
CGCATGATCATCGAAGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTTGTGAGACAA
GGTTCCCTCATTCAGGTGCCCATGTCTGAAAAGGGCAAGATCACCAGGGGGCGCCTGGGG
TCTCTCTCCCTAAAGAAAGAGGGCGAGCGACAGTGCTTCCTGTTTTCTAAGCATCTGATT
ATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACTTGACCAAGAATGGAGTCATATCCCTC
ATTGACTGCACTTTATTGGAGGAGCCAGAAAGCACGGAGGAGGAAGCCAAAGGATCCGGC
CAAGACATAGATCACTTGGATTTTAAAATCGGGGTGGAGCCAAAGGATTCCCCGCCCTTT
ACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAAGGCAGCGTGGACCAGTGACATCAGC
CAGTGTGTGGATAACATCCGATGCAATGGGCTCATGATGAACGCATTTGAAGAAAATTCC
AAGGTCACTGTGCCGCAGATGATCAAGTCCGACGCCTCCTTATATTGTGATGATGTTGAC
ATTCGCTTCAGCAAAACCATGAACTCCTGCAAAGTGCTGCAGATCCGCTACGCCAGTGTG
GAGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTTCCTGAGCATCGACTTCCTCAACACC
TTCCTGCACTCCTACCGCGTCTTCACCACCGCCATCGTGGTCCTGGACAAGCTCATTACC
ATCTACAAGAAGCCTATCAGTGCCATTCCTGCCAGGTCGCTGGAGCTCCTGTTTGCCAGT
GGCCAGAACAATAAGCTCCTGTACGGTGAACCCCCCAAGTCCCCGCGCGCCACCCGCAAG
TTCTCCTCGCCGCCACCTCTGTCCATCACCAAGACATCGTCACCGAGCCGCCGGCGGAAG
CTCTCCCTGAACATCCCCATCATCACTGGCGGCAAGGCCCTGGACCTGGCCGCCCTCAGC
TGCAACTCCAATGGCTACACCAGCATGTACTCGGCCATGTCACCCTTCAGCAAGGCCACG
CTGGACACCAGCAAGCTCTATGTGTCCAGCAGCTTCACCAACAAGATTCCAGATGAGGGC
GATACGACCCCTGAGAAGCCCGAAGACCCTTCAGCGCTCAGCAAGCAGAGCTCAGAAGTC
TCCATGAGAGAGGAGTCAGATATTGATCAAAACCAGAGTGATGATGGTGATACTGAAACA
TCACCAACTAAATCTCCAACAACACCCAAATCAGTCAAAAACAAAAATTCTTCAGAGTTC
CCACTCTTTTCCTATAACAATGGAGTCGTCATGACCTCCTGTCGTGAACTGGACAATAAC
CGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCATAGCAACCGCCGGGGCCAACGAGGGC
ACCCCAAACAAGGAGAAGTACCGGAGGATGTCCTTAGCCAGTGCAGGGTTTCCCCCAGAC
CAGAGGAATGGAGACAAGGAGTTTGTGATCCGCAGAGCAGCCACCAATCGTGTCTTGAAC
GTGCTCCGCCACTGGGTGTCCAAGCACTCTCAGGACTTTGAGACCAACGATGAGCTCAAA
TGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGCACGACCCGGAGCTCCTGACCCAGGAG
CGGAAGGCTGCAGCCAACATCATCAGGACTCTGACCCAGGAGGACCCAGGTGACAACCAG
ATCACGCTGGAGGAGATCACGCAGATGGCTGAAGGCGTGAAGGCTGAGCCCTTTGAAAAC
CACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCTGCTAGATCACCTCGTCTTCAAGAAG
ATTCCTTATGAGGAGTTCTTCGGACAAGGATGGATGAAACTGGAAAAGAATGAAAGGACC
CCTTATATCATGAAAACCACTAAGCACTTCAATGACATCAGTAACTTGATTGCTTCAGAA
ATCATCCGCAATGAGGACATCAACGCCAGGGTGAGCGCCATCGAGAAGTGGGTGGCCGTA
GCTGACATATGCCGCTGCCTCCACAACTACAATGCCGTACTGGAGATCACCTCGTCCATG
AACCGCAGTGCAATCTTCCGGCTCAAAAAGACGTGGCTCAAAGTCTCTAAGCAGACTAAA
GCTTTGATTGATAAGCTCCAAAAGCTTGTGTCATCTGAGGGCAGATTTAAGAATCTCAGA
GAAGCTCTGAAAAATTGTGACCCACCCTGTGTCCCTTACCTGGGGATGTACCTCACCGAC
CTGGCCTTCATCGAGGAGGGGACGCCCAATTACACGGAAGACGGCCTGGTCAACTTCTCC
AAGATGAGGATGATATCCCATATTATCCGAGAGATTCGCCAGTTTCAACAAACTGCCTAC
AAAATAGAGCACCAAGCAAAGGTAACGCAATATTTACTGGACCAATCTTTTGTAATGGAT
GAAGAAAGCCTCTACGAGTCTTCTCTCCGAATAGAACCAAAACTCCCCACC

ORF Protein

Cloned ORF protein sequence for pFN21AB9098 Download

>KIBB9098  1257 aa
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSS
RPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEW
VAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITS
LLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRK
RNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLH
QIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLL
KHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELS
RIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLG
SLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSG
QDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENS
KVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNT
FLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRK
FSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKAT
LDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTET
SPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEG
TPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELK
CKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFEN
HSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASE
IIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTK
ALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFS
KMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9098 Download

>pFN21AB9098  8476 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGAAGGGGATCCGGCTGAATGATGGCCACGT
CGCGTCCCTGGGACTGCTGGCGCGCAAGGACGGCACGCGCAAAGGCTACCTGAGCAAGCG
GAGTTCGGACAACACAAAATGGCAAACCAAGTGGTTCGCGCTGCTGCAGAACCTGCTCTT
CTACTTCGAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAGGGCTGCGT
CTGCGACCGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACA
GCATTACTTCACGGTGAACTTCAGCCATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGA
GGACGCAAAAGATTGTGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCCT
CGCCACAGAGCATGAGGCATTAATGCAGAAATACCTGCACCTGCTGCAGATCGTGGAGAC
AGAGAAGACCGTGGCCAAGCAGCTTCGGCAGCAGATCGAGGATGGGGAGATCGAGATCGA
GCGGCTGAAGGCAGAGATCACATCCCTGCTCAAGGACAATGAGCGCATCCAGTCCACCCA
GACTGTCGCCCCCAACGATGAAGACAGCGACATCAAGAAAATTAAGAAGGTGCAGAGCTT
CCTGCGGGGCTGGCTGTGCCGGCGGAAGTGGAAGACCATCATCCAGGACTACATCCGGTC
ACCCCATGCTGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGA
GGCTGAGTACGTGCAGCAGCTGCACATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGAT
GGCCGCCAGCTCCAAGAAGCCTCCCATCACACACGACGACGTCAGCAGCATCTTCCTGAA
CAGCGAAACCATCATGTTTTTACATCAGATCTTTTACCAAGGCCTGAAGGCCCGCATCTC
CAGCTGGCCCACGCTGGTCCTGGCTGACCTATTTGACATCCTGCTGCCCATGCTCAACAT
CTACCAAGAGTTCGTCCGCAACCACCAGTACAGCCTGCAGATCCTGGCCCACTGCAAGCA
GAACCGTGACTTCGACAAGCTGCTGAAGCACTACGAGGCCAAGCCTGACTGCGAGGAGAG
GACGCTGGAGACCTTCCTCACCTACCCCATGTTCCAGATCCCCAGGTACATCCTGACCCT
CCATGAGCTCCTGGCCCACACGCCTCATGAGCACGTTGAGCGCAACAGCCTGGACTACGC
CAAGTCCAAACTGGAGGAGCTGTCCAGAATAATGCACGATGAAGTAAGTGAGACGGAGAA
CATCCGGAAAAACCTGGCCATCGAGCGCATGATCATCGAAGGCTGTGAGATCCTCCTGGA
CACCAGCCAGACCTTTGTGAGACAAGGTTCCCTCATTCAGGTGCCCATGTCTGAAAAGGG
CAAGATCACCAGGGGGCGCCTGGGGTCTCTCTCCCTAAAGAAAGAGGGCGAGCGACAGTG
CTTCCTGTTTTCTAAGCATCTGATTATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACTT
GACCAAGAATGGAGTCATATCCCTCATTGACTGCACTTTATTGGAGGAGCCAGAAAGCAC
GGAGGAGGAAGCCAAAGGATCCGGCCAAGACATAGATCACTTGGATTTTAAAATCGGGGT
GGAGCCAAAGGATTCCCCGCCCTTTACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAA
GGCAGCGTGGACCAGTGACATCAGCCAGTGTGTGGATAACATCCGATGCAATGGGCTCAT
GATGAACGCATTTGAAGAAAATTCCAAGGTCACTGTGCCGCAGATGATCAAGTCCGACGC
CTCCTTATATTGTGATGATGTTGACATTCGCTTCAGCAAAACCATGAACTCCTGCAAAGT
GCTGCAGATCCGCTACGCCAGTGTGGAGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTT
CCTGAGCATCGACTTCCTCAACACCTTCCTGCACTCCTACCGCGTCTTCACCACCGCCAT
CGTGGTCCTGGACAAGCTCATTACCATCTACAAGAAGCCTATCAGTGCCATTCCTGCCAG
GTCGCTGGAGCTCCTGTTTGCCAGTGGCCAGAACAATAAGCTCCTGTACGGTGAACCCCC
CAAGTCCCCGCGCGCCACCCGCAAGTTCTCCTCGCCGCCACCTCTGTCCATCACCAAGAC
ATCGTCACCGAGCCGCCGGCGGAAGCTCTCCCTGAACATCCCCATCATCACTGGCGGCAA
GGCCCTGGACCTGGCCGCCCTCAGCTGCAACTCCAATGGCTACACCAGCATGTACTCGGC
CATGTCACCCTTCAGCAAGGCCACGCTGGACACCAGCAAGCTCTATGTGTCCAGCAGCTT
CACCAACAAGATTCCAGATGAGGGCGATACGACCCCTGAGAAGCCCGAAGACCCTTCAGC
GCTCAGCAAGCAGAGCTCAGAAGTCTCCATGAGAGAGGAGTCAGATATTGATCAAAACCA
GAGTGATGATGGTGATACTGAAACATCACCAACTAAATCTCCAACAACACCCAAATCAGT
CAAAAACAAAAATTCTTCAGAGTTCCCACTCTTTTCCTATAACAATGGAGTCGTCATGAC
CTCCTGTCGTGAACTGGACAATAACCGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCAT
AGCAACCGCCGGGGCCAACGAGGGCACCCCAAACAAGGAGAAGTACCGGAGGATGTCCTT
AGCCAGTGCAGGGTTTCCCCCAGACCAGAGGAATGGAGACAAGGAGTTTGTGATCCGCAG
AGCAGCCACCAATCGTGTCTTGAACGTGCTCCGCCACTGGGTGTCCAAGCACTCTCAGGA
CTTTGAGACCAACGATGAGCTCAAATGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGCA
CGACCCGGAGCTCCTGACCCAGGAGCGGAAGGCTGCAGCCAACATCATCAGGACTCTGAC
CCAGGAGGACCCAGGTGACAACCAGATCACGCTGGAGGAGATCACGCAGATGGCTGAAGG
CGTGAAGGCTGAGCCCTTTGAAAACCACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCT
GCTAGATCACCTCGTCTTCAAGAAGATTCCTTATGAGGAGTTCTTCGGACAAGGATGGAT
GAAACTGGAAAAGAATGAAAGGACCCCTTATATCATGAAAACCACTAAGCACTTCAATGA
CATCAGTAACTTGATTGCTTCAGAAATCATCCGCAATGAGGACATCAACGCCAGGGTGAG
CGCCATCGAGAAGTGGGTGGCCGTAGCTGACATATGCCGCTGCCTCCACAACTACAATGC
CGTACTGGAGATCACCTCGTCCATGAACCGCAGTGCAATCTTCCGGCTCAAAAAGACGTG
GCTCAAAGTCTCTAAGCAGACTAAAGCTTTGATTGATAAGCTCCAAAAGCTTGTGTCATC
TGAGGGCAGATTTAAGAATCTCAGAGAAGCTCTGAAAAATTGTGACCCACCCTGTGTCCC
TTACCTGGGGATGTACCTCACCGACCTGGCCTTCATCGAGGAGGGGACGCCCAATTACAC
GGAAGACGGCCTGGTCAACTTCTCCAAGATGAGGATGATATCCCATATTATCCGAGAGAT
TCGCCAGTTTCAACAAACTGCCTACAAAATAGAGCACCAAGCAAAGGTAACGCAATATTT
ACTGGACCAATCTTTTGTAATGGATGAAGAAAGCCTCTACGAGTCTTCTCTCCGAATAGA
ACCAAAACTCCCCACCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT