DMC1 ORF clone pFN21AB8648

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Halotag ORF Clone pFN21AB8648

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Clone info.

Species

human

Clone Name

pFN21AB8648 Sequence (with vector) Restriction Map

Product ID

FHC04793

Flexi Clone PID

FXC04793

ORF Sequence

DNA sequence (1020 bp)
Protein sequence (340 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587484]

Gene

KIBB8648

Symbol

DMC1

Alias : DMC1H, LIM15, dJ199H16.1

Description

DNA meiotic recombinase 1, transcript variant 1

Original Clone ID

oc00305

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1020 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 340 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13973.1 1.4e-154 100.0 DMC1 CCDS10062.1 5.4e-75 55.0 RAD51 CCDS53931.1 3e-63 50.1 RAD51 CCDS63477.1 3.5e-61 83.5 DMC1 CCDS53932.1 3.2e-49 50.6 RAD51 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013632 77 339 PF08423 DNA recombination and repair protein Rad51 HMMSmart IPR003593 118 307 SM00382 ATPase HMMTigr IPR011940 24 338 TIGR02238 Meiotic recombinase Dmc1 ProfileScan IPR020588 97 274 PS50162 DNA recombination/repair protein RecA/RadB IPR020587 277 340 PS50163 DNA recombination/repair protein RecA
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_008999 340 602721 100.0 100.0 XP_011528137 362 602721 97.6 96.2 XP_011528136 362 602721 97.6 96.2 XP_011520160 339 114480,179617,614508 55.0 95.6 NP_002866 339 114480,179617,614508 55.0 95.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8648 Download

>KIBB8648  1020 bp
ATGAAGGAGGATCAAGTTGTGGCGGAAGAACCAGGATTCCAAGATGAAGAGGAATCTTTG
TTTCAAGATATTGACCTGTTACAGAAACATGGAATTAACGTGGCTGACATTAAGAAACTG
AAATCAGTAGGAATCTGTACCATCAAAGGTATACAGATGACAACAAGAAGAGCTCTATGC
AATGTCAAAGGACTCTCAGAAGCCAAAGTAGACAAGATTAAAGAGGCAGCGAACAAACTA
ATTGAACCAGGATTCTTGACTGCATTTGAGTATAGTGAAAAGAGGAAAATGGTTTTCCAT
ATCACCACCGGGAGCCAGGAATTTGATAAGTTACTAGGAGGTGGAATTGAAAGTATGGCA
ATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACCCAGCTTTCTCATACCCTCTGT
GTGACAGCTCAACTTCCAGGAGCTGGTGGCTACCCAGGAGGAAAGATTATCTTCATTGAT
ACAGAAAATACTTTCCGTCCAGATCGCCTTAGGGACATTGCTGATCGCTTTAATGTAGAC
CATGATGCAGTACTGGACAACGTACTTTATGCACGTGCATATACTAGTGAACATCAGATG
GAGCTACTTGATTATGTAGCAGCAAAGTTCCATGAAGAAGCTGGCATCTTCAAGCTATTG
ATTATCGATTCAATAATGGCACTTTTTCGAGTGGATTTCAGTGGCCGTGGGGAGTTGGCC
GAACGGCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTCAGAAGAATAT
AACGTGGCTGTTTTTGTGACCAATCAAATGACTGCCGATCCAGGAGCAACTATGACCTTT
CAGGCAGATCCCAAAAAACCCATTGGGGGACACATTCTGGCTCATGCTTCAACAACAAGA
ATAAGCTTGCGAAAGGGAAGAGGAGAGCTCAGAATTGCCAAGATTTATGACAGTCCTGAG
ATGCCTGAAAATGAAGCCACCTTCGCAATAACTGCTGGAGGAATTGGGGATGCCAAGGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB8648 Download

>KIBB8648  340 aa
MKEDQVVAEEPGFQDEEESLFQDIDLLQKHGINVADIKKLKSVGICTIKGIQMTTRRALC
NVKGLSEAKVDKIKEAANKLIEPGFLTAFEYSEKRKMVFHITTGSQEFDKLLGGGIESMA
ITEAFGEFRTGKTQLSHTLCVTAQLPGAGGYPGGKIIFIDTENTFRPDRLRDIADRFNVD
HDAVLDNVLYARAYTSEHQMELLDYVAAKFHEEAGIFKLLIIDSIMALFRVDFSGRGELA
ERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTFQADPKKPIGGHILAHASTTR
ISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8648 Download

>pFN21AB8648  5725 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGGAGGATCAAGTTGTGGCGGAAGAACCAGG
ATTCCAAGATGAAGAGGAATCTTTGTTTCAAGATATTGACCTGTTACAGAAACATGGAAT
TAACGTGGCTGACATTAAGAAACTGAAATCAGTAGGAATCTGTACCATCAAAGGTATACA
GATGACAACAAGAAGAGCTCTATGCAATGTCAAAGGACTCTCAGAAGCCAAAGTAGACAA
GATTAAAGAGGCAGCGAACAAACTAATTGAACCAGGATTCTTGACTGCATTTGAGTATAG
TGAAAAGAGGAAAATGGTTTTCCATATCACCACCGGGAGCCAGGAATTTGATAAGTTACT
AGGAGGTGGAATTGAAAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAA
AACCCAGCTTTCTCATACCCTCTGTGTGACAGCTCAACTTCCAGGAGCTGGTGGCTACCC
AGGAGGAAAGATTATCTTCATTGATACAGAAAATACTTTCCGTCCAGATCGCCTTAGGGA
CATTGCTGATCGCTTTAATGTAGACCATGATGCAGTACTGGACAACGTACTTTATGCACG
TGCATATACTAGTGAACATCAGATGGAGCTACTTGATTATGTAGCAGCAAAGTTCCATGA
AGAAGCTGGCATCTTCAAGCTATTGATTATCGATTCAATAATGGCACTTTTTCGAGTGGA
TTTCAGTGGCCGTGGGGAGTTGGCCGAACGGCAGCAAAAATTGGCCCAGATGTTGTCACG
ACTCCAAAAAATCTCAGAAGAATATAACGTGGCTGTTTTTGTGACCAATCAAATGACTGC
CGATCCAGGAGCAACTATGACCTTTCAGGCAGATCCCAAAAAACCCATTGGGGGACACAT
TCTGGCTCATGCTTCAACAACAAGAATAAGCTTGCGAAAGGGAAGAGGAGAGCTCAGAAT
TGCCAAGATTTATGACAGTCCTGAGATGCCTGAAAATGAAGCCACCTTCGCAATAACTGC
TGGAGGAATTGGGGATGCCAAGGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT