LIMK2 ORF clone pFN21AB8450

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Halotag ORF Clone pFN21AB8450

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Clone info.

Species

human

Clone Name

pFN21AB8450 Sequence (with vector) Restriction Map

Product ID

FHC05834

Flexi Clone PID

FXC05834

ORF Sequence

DNA sequence (1914 bp)
Protein sequence (638 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587469]

Gene

KIBB8450

Symbol

LIMK2

Description

LIM domain kinase 2, transcript variant 2a

Original Clone ID

oc00203

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1914 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 638 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13891.1 9e-108 100.0 LIMK2 CCDS13892.1 2.9e-100 100.0 LIMK2 CCDS33637.1 2.6e-92 100.0 LIMK2 CCDS56491.1 5.6e-34 56.2 LIMK1 CCDS5563.1 5.8e-34 55.2 LIMK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001781 12 67 PF00412 Zinc finger IPR001781 72 126 PF00412 Zinc finger IPR001478 153 236 PF00595 PDZ/DHR/GLGF IPR001245 333 599 PF07714 Serine-threonine/tyrosine-protein kinase HMMSmart IPR001781 11 63 SM00132 Zinc finger IPR001781 71 124 SM00132 Zinc finger IPR001478 161 239 SM00228 PDZ/DHR/GLGF IPR002290 331 607 SM00220 Serine/threonine-protein kinase domain IPR020635 331 601 SM00219 Tyrosine-protein kinase ProfileScan IPR001781 10 70 PS50023 Zinc finger IPR001781 71 131 PS50023 Zinc finger IPR001478 152 239 PS50106 PDZ/DHR/GLGF IPR000719 331 608 PS50011 Protein kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005560 638 601988 100.0 100.0 NP_057952 617 601988 100.0 94.2 NP_001026971 686 601988 100.0 86.8 NP_001191355 613 601329 56.2 91.7 NP_002305 647 601329 55.2 96.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8450 Download

>KIBB8450  1914 bp
ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCT
CCAAGCCAGATATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGT
TCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGC
CCCAAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACA
GGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGC
TGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTAC
TGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG
TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAA
GGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTG
CAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCT
GGGGACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTG
GAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTC
TCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCC
GGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG
AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAG
GAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGC
TATTCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGC
TTCTTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAA
GAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATG
CGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAG
CTGAACCTGCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG
GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCC
TATTTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAG
TTGGACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGG
AAAAGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGC
AAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGA
AAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATT
GGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG
AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCC
GCCATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCC
TTTGAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAG
GAGTTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCC

ORF Protein

Cloned ORF protein sequence for pFN21AB8450 Download

>KIBB8450  638 aa
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYC
PKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLY
CGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTV
QVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPV
SQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPK
EPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMK
ELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSM
DPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEER
KRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEII
GQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDS
FEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8450 Download

>pFN21AB8450  6619 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTG
TCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAACGAAAC
CTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAACTGGTACTA
TGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTTTGGGGAGTTCTG
TCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCA
CCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGC
ACTGGTGCAGCATGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACC
CATGTTTGAGAGACTCTCCACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCT
CATCTCCATGCCGGCCACCACTGAAGGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGC
CTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGGTCAACCGGATGCACATCAGTCC
CAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAATGGGACCCCCGT
CCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTTCA
GCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCG
GCTCGCTCCTCACATGCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAA
GGAGAATCTGGAGGGGACACTGAGGAGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAA
GTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTC
AGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGATCTTCCGGCCCTGTGACCTAAT
CCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGACACACAAAGC
CACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAAAC
TTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCAT
TGGTGTGCTGTACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGGGGGCAC
ACTGAAGGACTTTCTGCGCAGTATGGATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGC
CAAAGGAATCGCCTCCGGAATGGCCTATTTGCACTCTATGTGCATCATCCACCGGGATCT
GAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTGTGGTGGTGGCAGACTTTGGGCT
GTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGCCACCACCAAGAA
ACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTACTG
GATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTT
TGGGATCGTTCTCTGTGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCG
AACACTGGACTTTGGCCTCAACGTGAAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTG
TCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCTGCAGACTGGAGCCTGAGAGCAGACC
AGCATTCTCGAAATTGGAGGACTCCTTTGAGGCCCTCTCCCTGTACCTGGGGGAGCTGGG
CATCCCGCTGCCTGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCATGCAGTACGGCCT
GACCCGGGACTCACCTCCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT