KCNJ4 ORF clone pFN21AB8448

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8448

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Clone info.

Species

human

Clone Name

pFN21AB8448 Sequence (with vector) Restriction Map

Product ID

FHC02845

Flexi Clone PID

FXC02845

ORF Sequence

DNA sequence (1335 bp)
Protein sequence (445 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463188]

Gene

KIBB8448

Symbol

KCNJ4

Alias : HIR, HIRK2, HRK1, IRK-3, IRK3, Kir2.3

Description

potassium channel, inwardly rectifying subfamily J, member 4, transcript variant 2

Original Clone ID

oc00201

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1335 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 445 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13971.1 3.9e-173 100.0 KCNJ4 CCDS11219.1 4.7e-81 65.0 KCNJ12 CCDS74015.1 2.9e-80 70.8 KCNJ18 CCDS11688.1 4.3e-77 60.9 KCNJ2 CCDS12721.1 4.9e-72 59.1 KCNJ14 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003273 1 14 PR01326 Potassium channel IPR013521 55 81 PR01320 Potassium channel IPR013521 124 146 PR01320 Potassium channel IPR003273 162 171 PR01326 Potassium channel IPR013521 253 271 PR01320 Potassium channel IPR003273 273 283 PR01326 Potassium channel IPR013521 284 304 PR01320 Potassium channel IPR013521 305 322 PR01320 Potassium channel IPR013521 329 343 PR01320 Potassium channel IPR003273 356 367 PR01326 Potassium channel IPR003273 376 384 PR01326 Potassium channel IPR003273 385 394 PR01326 Potassium channel IPR003273 397 406 PR01326 Potassium channel IPR003273 408 421 PR01326 Potassium channel HMMPfam IPR013521 22 364 PF01007 Potassium channel
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 62 FSAAFLVSWLFFGLLFWCIAFFH 84 PRIMARY 23 2 113 PCIMHVNGFLGAFLFSVETQTTI 135 SECONDARY 23 3 145 ECPLAVIAVVVQSIVGCVIDSFM 167 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_690607 445 600504 100.0 100.0 NP_004972 445 600504 100.0 100.0 XP_005256682 433 602323 65.0 98.7 NP_066292 433 602323 65.0 98.7 XP_011522133 433 602323 65.0 98.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8448 Download

>KIBB8448  1335 bp
ATGCACGGACACAGCCGCAACGGCCAGGCCCACGTGCCCCGGCGGAAGCGCCGCAACCGC
TTCGTCAAGAAGAACGGCCAATGCAACGTGTACTTCGCCAACCTGAGCAACAAGTCGCAG
CGCTACATGGCGGACATCTTCACCACCTGCGTGGACACGCGCTGGCGCTACATGCTCATG
ATCTTCTCCGCGGCCTTCCTTGTCTCCTGGCTCTTTTTCGGCCTCCTCTTCTGGTGTATC
GCCTTCTTCCACGGTGACCTGGAGGCCAGCCCAGGGGTGCCTGCGGCGGGGGGCCCGGCG
GCGGGTGGTGGCGGAGCAGCCCCGGTGGCCCCCAAGCCCTGCATCATGCACGTGAACGGC
TTCCTGGGTGCCTTCCTGTTCTCGGTGGAGACGCAGACGACCATCGGCTATGGGTTCCGG
TGCGTGACAGAGGAGTGCCCGCTGGCAGTCATCGCTGTGGTGGTCCAGTCCATCGTGGGC
TGCGTCATCGACTCCTTCATGATTGGCACCATCATGGCCAAGATGGCGCGGCCCAAGAAG
CGGGCGCAGACGTTGCTGTTCAGCCACCACGCGGTCATTTCGGTGCGCGACGGCAAGCTC
TGCCTCATGTGGCGCGTGGGCAACCTGCGCAAGAGCCACATTGTGGAGGCCCACGTGCGG
GCCCAGCTCATCAAGCCCTACATGACCCAGGAGGGCGAGTACCTGCCCCTGGACCAGCGG
GACCTCAACGTGGGCTATGACATCGGCCTGGACCGCATCTTCCTGGTGTCGCCCATCATC
ATTGTCCACGAGATCGACGAGGACAGCCCGCTTTATGGCATGGGCAAGGAGGAGCTGGAG
TCGGAGGACTTTGAGATCGTGGTCATCCTGGAGGGCATGGTGGAGGCCACGGCCATGACC
ACCCAGGCCCGCAGCTCCTACCTGGCCAGCGAGATCCTGTGGGGCCACCGCTTTGAGCCT
GTGGTCTTCGAGGAGAAGAGCCACTACAAGGTGGACTACTCGCGTTTTCACAAGACCTAC
GAGGTGGCCGGCACGCCCTGCTGCTCGGCCCGGGAGCTGCAGGAGAGTAAGATCACCGTG
CTGCCCGCCCCACCGCCCCCTCCCAGTGCCTTCTGCTACGAGAACGAGCTGGCCCTTATG
AGCCAGGAGGAAGAGGAGATGGAGGAGGAGGCAGCTGCGGCGGCCGCGGTGGCCGCAGGC
CTGGGCCTGGAGGCGGGTTCCAAGGAGGAGGCGGGCATCATCCGGATGCTGGAGTTCGGC
AGCCACCTGGACCTGGAGCGCATGCAGGCTTCCCTCCCGCTGGACAACATCTCCTACCGC
AGGGAGTCTGCCATC

ORF Protein

Cloned ORF protein sequence for pFN21AB8448 Download

>KIBB8448  445 aa
MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLM
IFSAAFLVSWLFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNG
FLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKK
RAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQR
DLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMT
TQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITV
LPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFG
SHLDLERMQASLPLDNISYRRESAI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8448 Download

>pFN21AB8448  6040 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCACGGACACAGCCGCAACGGCCAGGCCCACGT
GCCCCGGCGGAAGCGCCGCAACCGCTTCGTCAAGAAGAACGGCCAATGCAACGTGTACTT
CGCCAACCTGAGCAACAAGTCGCAGCGCTACATGGCGGACATCTTCACCACCTGCGTGGA
CACGCGCTGGCGCTACATGCTCATGATCTTCTCCGCGGCCTTCCTTGTCTCCTGGCTCTT
TTTCGGCCTCCTCTTCTGGTGTATCGCCTTCTTCCACGGTGACCTGGAGGCCAGCCCAGG
GGTGCCTGCGGCGGGGGGCCCGGCGGCGGGTGGTGGCGGAGCAGCCCCGGTGGCCCCCAA
GCCCTGCATCATGCACGTGAACGGCTTCCTGGGTGCCTTCCTGTTCTCGGTGGAGACGCA
GACGACCATCGGCTATGGGTTCCGGTGCGTGACAGAGGAGTGCCCGCTGGCAGTCATCGC
TGTGGTGGTCCAGTCCATCGTGGGCTGCGTCATCGACTCCTTCATGATTGGCACCATCAT
GGCCAAGATGGCGCGGCCCAAGAAGCGGGCGCAGACGTTGCTGTTCAGCCACCACGCGGT
CATTTCGGTGCGCGACGGCAAGCTCTGCCTCATGTGGCGCGTGGGCAACCTGCGCAAGAG
CCACATTGTGGAGGCCCACGTGCGGGCCCAGCTCATCAAGCCCTACATGACCCAGGAGGG
CGAGTACCTGCCCCTGGACCAGCGGGACCTCAACGTGGGCTATGACATCGGCCTGGACCG
CATCTTCCTGGTGTCGCCCATCATCATTGTCCACGAGATCGACGAGGACAGCCCGCTTTA
TGGCATGGGCAAGGAGGAGCTGGAGTCGGAGGACTTTGAGATCGTGGTCATCCTGGAGGG
CATGGTGGAGGCCACGGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAGCGAGAT
CCTGTGGGGCCACCGCTTTGAGCCTGTGGTCTTCGAGGAGAAGAGCCACTACAAGGTGGA
CTACTCGCGTTTTCACAAGACCTACGAGGTGGCCGGCACGCCCTGCTGCTCGGCCCGGGA
GCTGCAGGAGAGTAAGATCACCGTGCTGCCCGCCCCACCGCCCCCTCCCAGTGCCTTCTG
CTACGAGAACGAGCTGGCCCTTATGAGCCAGGAGGAAGAGGAGATGGAGGAGGAGGCAGC
TGCGGCGGCCGCGGTGGCCGCAGGCCTGGGCCTGGAGGCGGGTTCCAAGGAGGAGGCGGG
CATCATCCGGATGCTGGAGTTCGGCAGCCACCTGGACCTGGAGCGCATGCAGGCTTCCCT
CCCGCTGGACAACATCTCCTACCGCAGGGAGTCTGCCATCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT