PLEKHO1 ORF clone pFN21AB8228

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8228

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Clone info.

Species

human

Clone Name

pFN21AB8228 Sequence (with vector) Restriction Map

Product ID

FHC02679

Flexi Clone PID

FXC02679

ORF Sequence

DNA sequence (1227 bp)
Protein sequence (409 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464030]

Gene

KIBB8228

Symbol

PLEKHO1

Alias : CKIP-1, CKIP1, JBP, OC120

Description

pleckstrin homology domain containing, family O member 1, transcript variant 1

Original Clone ID

cp00588

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1227 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 409 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS945.1 1.2e-122 100.0 PLEKHO1 CCDS76210.1 9.3e-65 100.0 PLEKHO1 CCDS10196.1 1.1e-13 33.8 PLEKHO2 CCDS73739.1 1.5e-11 34.1 PLEKHO2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 24 131 PF00169 Pleckstrin homology domain HMMSmart IPR001849 22 134 SM00233 Pleckstrin homology domain ProfileScan IPR001849 21 132 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057358 409 608335 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8228 Download

>KIBB8228  1227 bp
ATGATGAAGAAGAACAATTCCGCCAAGCGGGGACCTCAGGATGGAAACCAGCAGCCTGCA
CCGCCCGAGAAGGTCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATT
TGGAAAAACCGCTATGTGGTGCTGAAAGGGGACCAGCTCTACATCTCTGAGAAGGAGGTA
AAAGATGAGAAAAATATTCAAGAGGTATTTGACCTGAGTGACTATGAGAAGTGTGAAGAG
CTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCATAGCAAGTTTACTCTTGCCCACTCC
AAACAGCCCGGTAACACGGCACCCAACCTGATCTTCCTGGCAGTGAGTCCAGAAGAGAAG
GAATCGTGGATCAATGCCCTCAACTCTGCCATCACCCGAGCCAAGAACCGTATCTTGGAT
GAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCATCCCACTCGAGACAGGGCAAAAATC
CAGCACTCCCGCCGCCCCCCAACAAGGGGACACCTAATGGCTGTGGCTTCCACCTCTACC
TCGGATGGGATGCTGACCTTGGACTTGATCCAAGAGGAAGACCCTTCCCCTGAGGAACCA
ACCTCTTGTGCTGAGAGCTTTCGGGTTGACCTGGACAAGTCTGTGGCCCAGCTGGCAGGG
AGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCCCTCCGCAGACCGGGCAAGCAGTCTC
TCCCGACCTTGGGAAAAAACAGACAAAGGGGCCACCTACACCCCCCAGGCACCCAAGAAG
TTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCTGGAGGAGATCCTATCTCAGCGGGAT
GCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGAGGAACCCCCAACCCCTGCCCTCCCC
AACCCGGGGCAGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGGAGACTCAG
GAGCTTCTGGCAGAGGTTCAGGGACTGGGAGATGGGAAGCGAAAGGCCAAGGACCCCCCT
CGGTCTCCGCCGGATTCTGAGTCAGAGCAGCTGCTGCTGGAGACGGAACGGCTGCTGGGA
GAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGTGCTGCAGGAGGTCAGGGAGCTGAGA
GACCTGTACAGACAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACAGACCACCCCG
CACAGTCAGTACCGGAAGAGCCTGATG

ORF Protein

Cloned ORF protein sequence for pFN21AB8228 Download

>KIBB8228  409 aa
MMKKNNSAKRGPQDGNQQPAPPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYISEKEV
KDEKNIQEVFDLSDYEKCEELRKSKSRSKKNHSKFTLAHSKQPGNTAPNLIFLAVSPEEK
ESWINALNSAITRAKNRILDEVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTST
SDGMLTLDLIQEEDPSPEEPTSCAESFRVDLDKSVAQLAGSRRRADSDRIQPSADRASSL
SRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRDAASARTLQLRAEEPPTPALP
NPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQLLLETERLLG
EASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8228 Download

>pFN21AB8228  5932 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGAAGAAGAACAATTCCGCCAAGCGGGGACC
TCAGGATGGAAACCAGCAGCCTGCACCGCCCGAGAAGGTCGGCTGGGTCCGGAAATTCTG
CGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGTGCTGAAAGGGGACCA
GCTCTACATCTCTGAGAAGGAGGTAAAAGATGAGAAAAATATTCAAGAGGTATTTGACCT
GAGTGACTATGAGAAGTGTGAAGAGCTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCA
TAGCAAGTTTACTCTTGCCCACTCCAAACAGCCCGGTAACACGGCACCCAACCTGATCTT
CCTGGCAGTGAGTCCAGAAGAGAAGGAATCGTGGATCAATGCCCTCAACTCTGCCATCAC
CCGAGCCAAGAACCGTATCTTGGATGAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCA
TCCCACTCGAGACAGGGCAAAAATCCAGCACTCCCGCCGCCCCCCAACAAGGGGACACCT
AATGGCTGTGGCTTCCACCTCTACCTCGGATGGGATGCTGACCTTGGACTTGATCCAAGA
GGAAGACCCTTCCCCTGAGGAACCAACCTCTTGTGCTGAGAGCTTTCGGGTTGACCTGGA
CAAGTCTGTGGCCCAGCTGGCAGGGAGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCC
CTCCGCAGACCGGGCAAGCAGTCTCTCCCGACCTTGGGAAAAAACAGACAAAGGGGCCAC
CTACACCCCCCAGGCACCCAAGAAGTTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCT
GGAGGAGATCCTATCTCAGCGGGATGCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGA
GGAACCCCCAACCCCTGCCCTCCCCAACCCGGGGCAGCTGTCCCGGATCCAGGACCTGGT
AGCAAGGAAACTGGAGGAGACTCAGGAGCTTCTGGCAGAGGTTCAGGGACTGGGAGATGG
GAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTCAGAGCAGCTGCT
GCTGGAGACGGAACGGCTGCTGGGAGAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGT
GCTGCAGGAGGTCAGGGAGCTGAGAGACCTGTACAGACAGATGGACCTGCAGACCCCGGA
CTCCCACCTCAGACAGACCACCCCGCACAGTCAGTACCGGAAGAGCCTGATGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT