PIAS4 ORF clone pFN21AB7782

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Halotag ORF Clone pFN21AB7782

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Clone info.

Species

human

Clone Name

pFN21AB7782 Sequence (with vector) Restriction Map

Product ID

FHC06356

Flexi Clone PID

FXC06356

ORF Sequence

DNA sequence (1530 bp)
Protein sequence (510 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464092]

Gene

KIBB7782

Symbol

PIAS4

Alias : PIAS-gamma, PIASY, Piasg, ZMIZ6

Description

protein inhibitor of activated STAT, 4

Original Clone ID

cs00282

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1530 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 510 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12118.1 5.7e-153 100.0 PIAS4 CCDS72866.1 7.8e-61 48.0 PIAS3 CCDS45290.1 6.5e-50 48.4 PIAS1 CCDS81902.1 6.5e-50 48.6 PIAS1 CCDS32825.1 3.6e-48 46.7 PIAS2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004181 322 371 PF02891 Zinc finger HMMSmart IPR003034 12 46 SM00513 DNA-binding SAP ProfileScan IPR003034 12 46 PS50800 DNA-binding SAP IPR023321 119 279 PS51466 PINIT domain IPR004181 311 388 PS51044 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056981 510 605989 100.0 100.0 XP_011526362 529 605989 100.0 98.4 NP_006090 628 605987 48.0 99.4 XP_016878179 642 603566 48.6 96.3 XP_016878180 642 603566 48.6 96.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7782 Download

>KIBB7782  1530 bp
ATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTT
CAGATGCTCCTGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACC
AGGGCCCTCCAGCTGGTGCAGTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAG
CTGTACGAGACCCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCC
CTGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCCAGGACTCCG
CTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAACGGACTGGGA
CGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAAT
ATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAG
GAGAGCCCGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGG
GAACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACC
AGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGTGAAGGTCAACCACAGCTAC
TGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGGTGGAGCCCAAGAGGCCGTGC
CGCCCCATCAACCTCACCCACCTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTC
ACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACC
TCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAG
GCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCACCACCGGTGTG
CGGGTGTCCCTCATCTGTCCGCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAG
ACCTGCGCCCACCTGCAGTGCTTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAG
CCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGAC
GGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAGTACCTGGTG
GACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCC
ATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGG
AGCGGTGCCCTGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAG
CCGGGCGCCGATGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAG
GAGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAGCGCCGCTGC
CCCTTCCAGAAGGGCCTGGTGCCGGCCTGC

ORF Protein

Cloned ORF protein sequence for pFN21AB7782 Download

>KIBB7782  510 aa
MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKE
LYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLG
RLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSR
ELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPC
RPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCK
ALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKK
PTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGA
ILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEE
EEEEEEEDEDEEGPRPKRRCPFQKGLVPAC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7782 Download

>pFN21AB7782  6235 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGT
GATGAGTTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTAAGAG
TGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAGTTTGACTGTAGCCC
TGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGCCAAGAAGAACTCGGA
GCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCG
GGCCGGCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTA
CGGAAAGTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCG
CCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGT
CCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGTGCATCTTCGCATTGACGCCAAGACA
GGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGT
CCTGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACAT
CGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCC
CGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACCCACCTCATGTACCTGTC
CTCGGCCACCAACCGCATCACTGTCACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGC
CCTGTACCTGGTGCGGCAGCTGACCTCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGG
GGTAAAGCACCCGGAGCTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGA
CAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGATGCG
GCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCCGTCTT
CTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGC
CCCCTACGACCAGCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGA
CGCCGACGAGATCGAGTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGA
GCGCAGCTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCT
CCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACGGGTGGCGGCGGCCC
GGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGACCTCACGCTGGACAG
CTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGA
GGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT