CERS6 ORF clone pFN21AB7648

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Halotag ORF Clone pFN21AB7648

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Clone info.

Species

human

Clone Name

pFN21AB7648 Sequence (with vector) Restriction Map

Product ID

FHC03880

Flexi Clone PID

FXC03880

ORF Sequence

DNA sequence (1176 bp)
Protein sequence (392 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527953]

Gene

KIBB7648

Symbol

CERS6

Alias : CERS5, LASS6

Description

ceramide synthase 6, transcript variant 1

Original Clone ID

cs00148

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1176 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 392 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS58734.1 8e-174 100.0 CERS6 CCDS2228.1 8.9e-168 98.0 CERS6 CCDS8801.1 1e-110 63.8 CERS5 CCDS61120.1 7.2e-98 65.5 CERS5 CCDS973.1 9.7e-64 43.0 CERS2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001356 82 125 PF00046 Homeobox IPR006634 131 324 PF03798 TRAM/LAG1/CLN8 homology domain HMMSmart IPR006634 130 331 SM00724 TRAM/LAG1/CLN8 homology domain ProfileScan IPR001356 83 127 PS50071 Homeobox IPR006634 130 331 PS50922 TRAM/LAG1/CLN8 homology domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 35 AEDLYLAFPLAFCIFMVRLIFER 57 PRIMARY 23 2 204 DFGIMFLHHLVSIFLITFSYVNN 226 SECONDARY 23 3 264 FVMFAVVFITTRLGIFPLWVLNT 286 PRIMARY 23 4 305 NLLLLLVQGLNCFWSYLIVKIAC 327 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001243055 392 615336 100.0 100.0 NP_982288 384 615336 98.0 100.0 NP_671723 392 615335 63.8 96.2 XP_005269277 409 615335 60.8 93.6 NP_001318000 381 615335 65.5 85.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7648 Download

>KIBB7648  1176 bp
ATGGCAGGGATCTTAGCCTGGTTCTGGAACGAGAGGTTTTGGCTCCCGCACAATGTCACC
TGGGCGGACCTGAAGAACACGGAGGAGGCCACCTTCCCGCAGGCTGAGGACCTCTATCTC
GCTTTTCCCCTGGCCTTCTGTATCTTCATGGTGCGGCTCATCTTCGAGAGATTTGTAGCC
AAACCGTGCGCCATAGCCCTCAACATTCAGGCCAATGGACCACAAATTGCTCCGCCCAAT
GCCATTCTGGAAAAGGTCTTCACTGCAATTACAAAGCATCCTGATGAAAAGAGATTGGAA
GGCCTCTCCAAGCAACTGGACTGGGATGTTCGAAGCATTCAGCGCTGGTTTCGACAAAGA
CGCAATCAGGAGAAGCCAAGCACGCTGACGAGGTTCTGTGAGAGCATGTGGAGATTTTCA
TTTTACCTTTATGTATTTACCTACGGAGTCAGATTCCTGAAAAAGACCCCCTGGTTGTGG
AATACGAGGCATTGCTGGTACAACTACCCCTATCAGCCACTCACAACTGACCTTCACTAC
TATTACATCCTGGAGCTGTCGTTTTATTGGTCTTTGATGTTTTCTCAGTTCACTGATATC
AAAAGAAAGGACTTTGGCATTATGTTCCTGCACCACCTTGTATCTATTTTCTTGATTACC
TTTTCATATGTCAACAATATGGCCCGAGTAGGAACGCTGGTCCTTTGTCTTCATGATTCA
GCTGATGCTCTTCTGGAGGCTGCCAAAATGGCAAATTATGCCAAGTTTCAGAAAATGTGT
GATCTCCTGTTTGTTATGTTTGCCGTGGTTTTTATCACCACACGACTGGGTATATTTCCT
CTCTGGGTGTTAAATACCACATTATTTGAAAGCTGGGAGATCGTTGGACCTTACCCTTCC
TGGTGGGTTTTTAACCTACTGCTATTGCTAGTACAAGGGTTGAACTGCTTCTGGTCTTAC
TTGATTGTGAAAATAGCTTGCAAAGCTGTTTCAAGAGGCAAGGCTGGGAAGTGGAACCCT
TTACATGTGTCCAAGGATGATCGAAGTGATATTGAGTCTAGCTCAGATGAGGAGGACTCA
GAACCTCCGGGAAAGAATCCCCACACTGCGACAACCACCAATGGGACCAGTGGTACCAAC
GGGTATCTCCTGACTGGCTCCTGCTCCATGGATGAT

ORF Protein

Cloned ORF protein sequence for pFN21AB7648 Download

>KIBB7648  392 aa
MAGILAWFWNERFWLPHNVTWADLKNTEEATFPQAEDLYLAFPLAFCIFMVRLIFERFVA
KPCAIALNIQANGPQIAPPNAILEKVFTAITKHPDEKRLEGLSKQLDWDVRSIQRWFRQR
RNQEKPSTLTRFCESMWRFSFYLYVFTYGVRFLKKTPWLWNTRHCWYNYPYQPLTTDLHY
YYILELSFYWSLMFSQFTDIKRKDFGIMFLHHLVSIFLITFSYVNNMARVGTLVLCLHDS
ADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRLGIFPLWVLNTTLFESWEIVGPYPS
WWVFNLLLLLVQGLNCFWSYLIVKIACKAVSRGKAGKWNPLHVSKDDRSDIESSSDEEDS
EPPGKNPHTATTTNGTSGTNGYLLTGSCSMDD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7648 Download

>pFN21AB7648  5881 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGGATCTTAGCCTGGTTCTGGAACGAGAG
GTTTTGGCTCCCGCACAATGTCACCTGGGCGGACCTGAAGAACACGGAGGAGGCCACCTT
CCCGCAGGCTGAGGACCTCTATCTCGCTTTTCCCCTGGCCTTCTGTATCTTCATGGTGCG
GCTCATCTTCGAGAGATTTGTAGCCAAACCGTGCGCCATAGCCCTCAACATTCAGGCCAA
TGGACCACAAATTGCTCCGCCCAATGCCATTCTGGAAAAGGTCTTCACTGCAATTACAAA
GCATCCTGATGAAAAGAGATTGGAAGGCCTCTCCAAGCAACTGGACTGGGATGTTCGAAG
CATTCAGCGCTGGTTTCGACAAAGACGCAATCAGGAGAAGCCAAGCACGCTGACGAGGTT
CTGTGAGAGCATGTGGAGATTTTCATTTTACCTTTATGTATTTACCTACGGAGTCAGATT
CCTGAAAAAGACCCCCTGGTTGTGGAATACGAGGCATTGCTGGTACAACTACCCCTATCA
GCCACTCACAACTGACCTTCACTACTATTACATCCTGGAGCTGTCGTTTTATTGGTCTTT
GATGTTTTCTCAGTTCACTGATATCAAAAGAAAGGACTTTGGCATTATGTTCCTGCACCA
CCTTGTATCTATTTTCTTGATTACCTTTTCATATGTCAACAATATGGCCCGAGTAGGAAC
GCTGGTCCTTTGTCTTCATGATTCAGCTGATGCTCTTCTGGAGGCTGCCAAAATGGCAAA
TTATGCCAAGTTTCAGAAAATGTGTGATCTCCTGTTTGTTATGTTTGCCGTGGTTTTTAT
CACCACACGACTGGGTATATTTCCTCTCTGGGTGTTAAATACCACATTATTTGAAAGCTG
GGAGATCGTTGGACCTTACCCTTCCTGGTGGGTTTTTAACCTACTGCTATTGCTAGTACA
AGGGTTGAACTGCTTCTGGTCTTACTTGATTGTGAAAATAGCTTGCAAAGCTGTTTCAAG
AGGCAAGGCTGGGAAGTGGAACCCTTTACATGTGTCCAAGGATGATCGAAGTGATATTGA
GTCTAGCTCAGATGAGGAGGACTCAGAACCTCCGGGAAAGAATCCCCACACTGCGACAAC
CACCAATGGGACCAGTGGTACCAACGGGTATCTCCTGACTGGCTCCTGCTCCATGGATGA
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T