MARCH1 ORF clone pFN21AB7447

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Halotag ORF Clone pFN21AB7447

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Clone info.

Species

human

Clone Name

pFN21AB7447 Sequence (with vector) Restriction Map

Product ID

FHC02473

Flexi Clone PID

FXC02473

ORF Sequence

DNA sequence (816 bp)
Protein sequence (272 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463878]

Gene

KIBB7447

Symbol

MARCH1

Description

membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase, transcript variant 2

Original Clone ID

oc00661

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 816 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 272 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3806.1 7.1e-114 100.0 MARCH1 CCDS54814.1 4.4e-98 96.3 MARCH1 CCDS7213.1 1.9e-69 67.8 MARCH8 CCDS60519.1 2.4e-65 70.1 MARCH8 CCDS12202.1 4.1e-13 30.7 MARCH2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 63 109 PF00097 Zinc finger HMMSmart IPR011016 62 110 SM00744 Zinc finger ProfileScan IPR011016 55 116 PS51292 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 140 CSVTFHVIAITCVVWSLYVLIDR 162 PRIMARY 23 2 185 VVVAIGFTGGLVFMYVQCKVYVQ 207 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060393 272 613331 100.0 100.0 XP_016863824 285 613331 91.6 96.0 XP_016863825 285 613331 91.6 96.0 XP_016863823 289 613331 96.3 90.4 NP_001159845 289 613331 96.3 90.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7447 Download

>KIBB7447  816 bp
ATGACCAGCAGCCACGTTTGTTGTAATTTTTTAAATATGTGGAAAAAAAGCAAGATATCA
ACCATGTATTACCTTAACCAAGATGCCAAATTATCTAACTTGTTTCTCCAGGCAAGCAGC
CCAACAACAGGGACAGCTCCCAGGAGCCAGTCAAGGTTGTCTGTCTGTCCATCCACTCAG
GACATCTGCAGAATCTGTCACTGCGAAGGGGATGAAGAGAGCCCCCTCATCACACCCTGT
CGCTGCACTGGGACACTGCGCTTTGTCCACCAGTCCTGCCTCCACCAGTGGATAAAGAGC
TCAGATACACGCTGCTGTGAGCTCTGCAAGTATGACTTCATAATGGAGACCAAGCTCAAA
CCCCTCCGGAAGTGGGAGAAACTACAGATGACCACAAGTGAAAGGAGGAAAATATTCTGC
TCTGTCACATTCCACGTAATCGCGATCACCTGTGTGGTTTGGTCTTTGTATGTATTGATA
GACCGGACAGCGGAGGAAATCAAGCAAGGCAATGACAATGGTGTCCTTGAATGGCCATTT
TGGACAAAACTGGTTGTGGTAGCCATTGGCTTCACAGGAGGTCTTGTCTTCATGTACGTA
CAGTGTAAAGTCTATGTTCAGTTGTGGCGCAGGCTGAAGGCCTACAACCGTGTGATCTTT
GTACAAAATTGCCCAGACACTGCCAAAAAACTGGAGAAGAACTTCTCATGTAATGTAAAC
ACAGACATCAAAGATGCTGTGGTAGTGCCTGTACCACAAACAGGTGCAAATTCACTGCCA
TCTGCAGAGGGTGGCCCCCCTGAAGTTGTATCAGTC

ORF Protein

Cloned ORF protein sequence for pFN21AB7447 Download

>KIBB7447  272 aa
MTSSHVCCNFLNMWKKSKISTMYYLNQDAKLSNLFLQASSPTTGTAPRSQSRLSVCPSTQ
DICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDTRCCELCKYDFIMETKLK
PLRKWEKLQMTTSERRKIFCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGNDNGVLEWPF
WTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQNCPDTAKKLEKNFSCNVN
TDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7447 Download

>pFN21AB7447  5521 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCAGCAGCCACGTTTGTTGTAATTTTTTAAA
TATGTGGAAAAAAAGCAAGATATCAACCATGTATTACCTTAACCAAGATGCCAAATTATC
TAACTTGTTTCTCCAGGCAAGCAGCCCAACAACAGGGACAGCTCCCAGGAGCCAGTCAAG
GTTGTCTGTCTGTCCATCCACTCAGGACATCTGCAGAATCTGTCACTGCGAAGGGGATGA
AGAGAGCCCCCTCATCACACCCTGTCGCTGCACTGGGACACTGCGCTTTGTCCACCAGTC
CTGCCTCCACCAGTGGATAAAGAGCTCAGATACACGCTGCTGTGAGCTCTGCAAGTATGA
CTTCATAATGGAGACCAAGCTCAAACCCCTCCGGAAGTGGGAGAAACTACAGATGACCAC
AAGTGAAAGGAGGAAAATATTCTGCTCTGTCACATTCCACGTAATCGCGATCACCTGTGT
GGTTTGGTCTTTGTATGTATTGATAGACCGGACAGCGGAGGAAATCAAGCAAGGCAATGA
CAATGGTGTCCTTGAATGGCCATTTTGGACAAAACTGGTTGTGGTAGCCATTGGCTTCAC
AGGAGGTCTTGTCTTCATGTACGTACAGTGTAAAGTCTATGTTCAGTTGTGGCGCAGGCT
GAAGGCCTACAACCGTGTGATCTTTGTACAAAATTGCCCAGACACTGCCAAAAAACTGGA
GAAGAACTTCTCATGTAATGTAAACACAGACATCAAAGATGCTGTGGTAGTGCCTGTACC
ACAAACAGGTGCAAATTCACTGCCATCTGCAGAGGGTGGCCCCCCTGAAGTTGTATCAGT
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T