MRGPRD ORF clone pFN21AB7390

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Halotag ORF Clone pFN21AB7390

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Clone info.

Species

human

Clone Name

pFN21AB7390 Sequence (with vector) Restriction Map

Product ID

FHC02418

Flexi Clone PID

FXC02418

ORF Sequence

DNA sequence (963 bp)
Protein sequence (321 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463856]

Gene

KIBB7390

Symbol

MRGPRD

Alias : MRGD, TGR7

Description

MAS-related GPR, member D

Original Clone ID

oc00604

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 963 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 321 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31625.1 5.6e-112 100.0 MRGPRD CCDS7831.1 4.9e-35 42.8 MRGPRX4 CCDS7846.1 1.8e-34 42.1 MRGPRX1 CCDS7847.1 1.2e-33 43.1 MRGPRX2 CCDS7830.1 2.5e-32 40.4 MRGPRX3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 29 53 PR00237 GPCR IPR000276 61 82 PR00237 GPCR IPR000276 105 127 PR00237 GPCR IPR000276 219 243 PR00237 GPCR IPR000276 258 284 PR00237 GPCR HMMPfam IPR000276 44 276 PF00001 GPCR ProfileScan IPR017452 44 276 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 28 LVLSSLAMFTCLCGMAGNSMVIW 50 SECONDARY 23 2 63 IYILNLAAADLLFLFSMASTLSL 85 SECONDARY 23 3 106 YFAYTVGLSLLTAISTQRCLSVL 128 SECONDARY 23 4 140 HLSAWVCGLLWTLCLLMNGLTSS 162 PRIMARY 23 5 181 VQAALIMGVLTPVMTLSSLTLFV 203 SECONDARY 23 6 222 VVLASVLVFLICSLPLSIYWFVL 244 PRIMARY 23 7 260 LSRLSSSVSSSANPVIYFLVGS 281 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_944605 321 607231 100.0 100.0 NP_473373 322 607230 42.8 96.6 NP_671732 322 607227 42.1 97.2 NP_001290544 330 607228 43.1 89.4 NP_473371 330 607228 43.1 89.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7390 Download

>KIBB7390  963 bp
ATGAACCAGACTTTGAATAGCAGTGGGACCGTGGAGTCAGCCCTAAACTATTCCAGAGGG
AGCACAGTGCACACGGCCTACCTGGTGCTGAGCTCCCTGGCCATGTTCACCTGCCTGTGC
GGGATGGCAGGCAACAGCATGGTGATCTGGCTGCTGGGCTTTCGAATGCACAGGAACCCC
TTCTGCATCTATATCCTCAACCTGGCGGCAGCCGACCTCCTCTTCCTCTTCAGCATGGCT
TCCACGCTCAGCCTGGAAACCCAGCCCCTGGTCAATACCACTGACAAGGTCCACGAGCTG
ATGAAGAGACTGATGTACTTTGCCTACACAGTGGGCCTGAGCCTGCTGACGGCCATCAGC
ACCCAGCGCTGTCTCTCTGTCCTCTTCCCTATCTGGTTCAAGTGTCACCGGCCCAGGCAC
CTGTCAGCCTGGGTGTGTGGCCTGCTGTGGACACTCTGTCTCCTGATGAACGGGTTGACC
TCTTCCTTCTGCAGCAAGTTCTTGAAATTCAATGAAGATCGGTGCTTCAGGGTGGACATG
GTCCAGGCCGCCCTCATCATGGGGGTCTTAACCCCAGTGATGACTCTGTCCAGCCTGACC
CTCTTTGTCTGGGTGCGGAGGAGCTCCCAGCAGTGGCGGCGGCAGCCCACACGGCTGTTC
GTGGTGGTCCTGGCCTCTGTCCTGGTGTTCCTCATCTGTTCCCTGCCTCTGAGCATCTAC
TGGTTTGTGCTCTACTGGTTGAGCCTGCCGCCCGAGATGCAGGTCCTGTGCTTCAGCTTG
TCACGCCTCTCCTCGTCCGTAAGCAGCAGCGCCAACCCCGTCATCTACTTCCTGGTGGGC
AGCCGGAGGAGCCACAGGCTGCCCACCAGGTCCCTGGGGACTGTGCTCCAACAGGCGCTT
CGCGAGGAGCCCGAGCTGGAAGGTGGGGAGACGCCCACCGTGGGCACCAATGAGATGGGG
GCT

ORF Protein

Cloned ORF protein sequence for pFN21AB7390 Download

>KIBB7390  321 aa
MNQTLNSSGTVESALNYSRGSTVHTAYLVLSSLAMFTCLCGMAGNSMVIWLLGFRMHRNP
FCIYILNLAAADLLFLFSMASTLSLETQPLVNTTDKVHELMKRLMYFAYTVGLSLLTAIS
TQRCLSVLFPIWFKCHRPRHLSAWVCGLLWTLCLLMNGLTSSFCSKFLKFNEDRCFRVDM
VQAALIMGVLTPVMTLSSLTLFVWVRRSSQQWRRQPTRLFVVVLASVLVFLICSLPLSIY
WFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVGSRRSHRLPTRSLGTVLQQAL
REEPELEGGETPTVGTNEMGA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7390 Download

>pFN21AB7390  5668 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACCAGACTTTGAATAGCAGTGGGACCGTGGA
GTCAGCCCTAAACTATTCCAGAGGGAGCACAGTGCACACGGCCTACCTGGTGCTGAGCTC
CCTGGCCATGTTCACCTGCCTGTGCGGGATGGCAGGCAACAGCATGGTGATCTGGCTGCT
GGGCTTTCGAATGCACAGGAACCCCTTCTGCATCTATATCCTCAACCTGGCGGCAGCCGA
CCTCCTCTTCCTCTTCAGCATGGCTTCCACGCTCAGCCTGGAAACCCAGCCCCTGGTCAA
TACCACTGACAAGGTCCACGAGCTGATGAAGAGACTGATGTACTTTGCCTACACAGTGGG
CCTGAGCCTGCTGACGGCCATCAGCACCCAGCGCTGTCTCTCTGTCCTCTTCCCTATCTG
GTTCAAGTGTCACCGGCCCAGGCACCTGTCAGCCTGGGTGTGTGGCCTGCTGTGGACACT
CTGTCTCCTGATGAACGGGTTGACCTCTTCCTTCTGCAGCAAGTTCTTGAAATTCAATGA
AGATCGGTGCTTCAGGGTGGACATGGTCCAGGCCGCCCTCATCATGGGGGTCTTAACCCC
AGTGATGACTCTGTCCAGCCTGACCCTCTTTGTCTGGGTGCGGAGGAGCTCCCAGCAGTG
GCGGCGGCAGCCCACACGGCTGTTCGTGGTGGTCCTGGCCTCTGTCCTGGTGTTCCTCAT
CTGTTCCCTGCCTCTGAGCATCTACTGGTTTGTGCTCTACTGGTTGAGCCTGCCGCCCGA
GATGCAGGTCCTGTGCTTCAGCTTGTCACGCCTCTCCTCGTCCGTAAGCAGCAGCGCCAA
CCCCGTCATCTACTTCCTGGTGGGCAGCCGGAGGAGCCACAGGCTGCCCACCAGGTCCCT
GGGGACTGTGCTCCAACAGGCGCTTCGCGAGGAGCCCGAGCTGGAAGGTGGGGAGACGCC
CACCGTGGGCACCAATGAGATGGGGGCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT