PAPOLA ORF clone pFN21AB7274

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB7274

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Clone info.

Species

human

Clone Name

pFN21AB7274 Sequence (with vector) Restriction Map

Product ID

FHC10785

Flexi Clone PID

FXC10785

ORF Sequence

DNA sequence (2235 bp)
Protein sequence (745 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587439]

Gene

KIBB7274

Symbol

PAPOLA

Alias : PAP, PAP-alpha

Description

poly(A) polymerase alpha, transcript variant 1

Original Clone ID

ej01005m1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2235 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 745 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9946.1 3.4e-171 100.0 PAPOLA CCDS78202.1 2.3e-123 86.1 PAPOLB CCDS1863.1 2.2e-97 61.2 PAPOLG CCDS58334.1 2.4e-62 100.0 PAPOLA CCDS58335.1 5.1e-44 90.1 PAPOLA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007012 18 364 PF04928 Poly(A) polymerase IPR002934 94 174 PF01909 Nucleotidyl transferase domain IPR007010 366 507 PF04926 Poly(A) polymerase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_116021 745 605553 100.0 100.0 NP_001280556 744 605553 99.9 100.0 XP_005267339 699 605553 93.8 100.0 XP_005267338 724 605553 97.2 100.0 XP_016876425 698 605553 93.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7274 Download

>KIBB7274  2235 bp
ATGCCGTTTCCAGTTACAACACAGGGATCACAACAAACACAACCGCCACAGAAGCACTAT
GGCATTACTTCTCCTATCAGCTTAGCAGCCCCCAAGGAGACTGACTGCGTACTTACACAG
AAACTAATTGAGACATTGAAACCCTTTGGGGTTTTTGAAGAGGAAGAGGAACTGCAGCGC
AGGATTTTAATTTTGGGAAAACTAAATAACCTGGTAAAAGAGTGGATACGAGAAATCAGT
GAAAGCAAGAATCTTCCACAATCTGTAATTGAAAATGTTGGAGGAAAAATTTTTACATTT
GGATCTTACAGATTAGGAGTGCATACAAAAGGTGCTGATATTGATGCGTTGTGTGTTGCA
CCAAGACATGTTGATCGAAGTGACTTTTTCACCTCATTCTATGATAAGTTGAAATTACAG
GAAGAAGTAAAAGATTTAAGAGCTGTTGAAGAGGCATTCGTACCAGTTATTAAACTCTGT
TTTGATGGGATAGAGATTGATATTTTGTTTGCAAGATTAGCACTGCAGACAATTCCTGAA
GATTTGGATCTACGAGATGACAGTCTGCTAAAAAATTTAGATATAAGATGTATAAGAAGT
CTTAACGGTTGCAGGGTAACCGATGAAATTTTACATCTAGTACCAAACATTGACAACTTC
AGGTTAACTCTGAGAGCTATCAAACTATGGGCCAAACGCCACAACATCTATTCCAATATA
TTAGGTTTCCTCGGTGGTGTTTCCTGGGCTATGCTAGTAGCAAGAACTTGCCAGCTTTAT
CCAAATGCAATAGCATCAACTCTTGTACATAAATTTTTCTTGGTATTTTCTAAATGGGAA
TGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGAATGCAATCTTAATTTGCCTGTATGG
GACCCAAGGGTAAACCCCAGTGATAGGTACCATCTTATGCCTATAATTACACCAGCATAC
CCACAACAGAACTCCACGTACAATGTGTCCGTTTCAACACGGATGGTCATGGTTGAGGAG
TTCAAACAAGGTCTTGCTATCACAGATGAAATTTTGCTGAGTAAGGCAGAGTGGTCCAAA
CTTTTTGAAGCTCCAAACTTCTTTCAAAAGTACAAGCATTATATTGTACTTCTAGCAAGT
GCACCAACAGAAAAACAACGCCTGGAATGGGTGGGCTTGGTGGAATCAAAAATCCGAATC
CTGGTTGGAAGCTTGGAGAAGAATGAATTTATTACACTGGCTCATGTGAATCCCCAGTCA
TTTCCAGCACCCAAAGAAAATCCCGACAAGGAAGAATTTCGCACGATGTGGGTGATTGGG
TTAGTGTTTAAAAAAACAGAAAACTCTGAAAACCTCAGTGTTGATCTCACCTATGATATT
CAGTCTTTCACAGATACAGTTTATAGGCAAGCAATAAACAGCAAGATGTTTGAGGTGGAT
ATGAAAATTGCTGCAATGCATGTAAAAAGAAAGCAACTCCATCAACTACTACCTAATCAT
GTGCTTCAGAAAAAGAAAAAGCATTCAACAGAAGGTGTCAAATTGACAGCTCTCAATGAC
AGCAGCCTCGACTTGTCTATGGACAGTGATAACAGCATGTCTGTGCCTTCACCTACTAGT
GCTACGAAGACCAGTCCATTGAACAGTTCTGGCAGCTCTCAGGGCAGAAACAGTCCTGCT
CCAGCTGTAACAGCAGCATCTGTGACCAACATACAGGCTACTGAAGTTTCTGTGCCACAA
GTAAATTCCAGTGAAAGCTCAGGGGGTACATCGAGTGAAAGCATTCCTCAAACTGCCACA
CAACCAGCCATTTCTCCACCACCAAAGCCTACGGTCTCCAGAGTTGTTTCTTCAACACGT
CTGGTAAACCCACCACCTAGATCTTCAGGAAATGCAGCAACTTCAGGAAATGCAGCAACA
AAAATACCTACTCCTATAGTAGGAGTCAAGAGGACATCCTCACCTCATAAAGAAGAGAGT
CCCAAGAAAACCAAAACAGAAGAGGATGAAACAAGTGAAGATGCTAACTGTCTTGCTTTG
AGTGGACATGATAAAACAGAAGCAAAGGAACAACTTGATACAGAGACAAGTACAACTCAA
TCAGAAACTATTCAGACAGCGGCTTCTCTGTTGGCCTCTCAGAAAACATCCAGTACAGAC
CTTTCTGATATCCCTGCTCTCCCTGCAAATCCTATTCCTGTTATCAAGAATTCAATAAAA
CTGAGATTGAATCGG

ORF Protein

Cloned ORF protein sequence for pFN21AB7274 Download

>KIBB7274  745 aa
MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQR
RILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVA
PRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPE
DLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNI
LGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVW
DPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSK
LFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQS
FPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVD
MKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTS
ATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTAT
QPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEES
PKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTD
LSDIPALPANPIPVIKNSIKLRLNR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7274 Download

>pFN21AB7274  6940 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGTTTCCAGTTACAACACAGGGATCACAACA
AACACAACCGCCACAGAAGCACTATGGCATTACTTCTCCTATCAGCTTAGCAGCCCCCAA
GGAGACTGACTGCGTACTTACACAGAAACTAATTGAGACATTGAAACCCTTTGGGGTTTT
TGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGGGAAAACTAAATAACCTGGT
AAAAGAGTGGATACGAGAAATCAGTGAAAGCAAGAATCTTCCACAATCTGTAATTGAAAA
TGTTGGAGGAAAAATTTTTACATTTGGATCTTACAGATTAGGAGTGCATACAAAAGGTGC
TGATATTGATGCGTTGTGTGTTGCACCAAGACATGTTGATCGAAGTGACTTTTTCACCTC
ATTCTATGATAAGTTGAAATTACAGGAAGAAGTAAAAGATTTAAGAGCTGTTGAAGAGGC
ATTCGTACCAGTTATTAAACTCTGTTTTGATGGGATAGAGATTGATATTTTGTTTGCAAG
ATTAGCACTGCAGACAATTCCTGAAGATTTGGATCTACGAGATGACAGTCTGCTAAAAAA
TTTAGATATAAGATGTATAAGAAGTCTTAACGGTTGCAGGGTAACCGATGAAATTTTACA
TCTAGTACCAAACATTGACAACTTCAGGTTAACTCTGAGAGCTATCAAACTATGGGCCAA
ACGCCACAACATCTATTCCAATATATTAGGTTTCCTCGGTGGTGTTTCCTGGGCTATGCT
AGTAGCAAGAACTTGCCAGCTTTATCCAAATGCAATAGCATCAACTCTTGTACATAAATT
TTTCTTGGTATTTTCTAAATGGGAATGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGA
ATGCAATCTTAATTTGCCTGTATGGGACCCAAGGGTAAACCCCAGTGATAGGTACCATCT
TATGCCTATAATTACACCAGCATACCCACAACAGAACTCCACGTACAATGTGTCCGTTTC
AACACGGATGGTCATGGTTGAGGAGTTCAAACAAGGTCTTGCTATCACAGATGAAATTTT
GCTGAGTAAGGCAGAGTGGTCCAAACTTTTTGAAGCTCCAAACTTCTTTCAAAAGTACAA
GCATTATATTGTACTTCTAGCAAGTGCACCAACAGAAAAACAACGCCTGGAATGGGTGGG
CTTGGTGGAATCAAAAATCCGAATCCTGGTTGGAAGCTTGGAGAAGAATGAATTTATTAC
ACTGGCTCATGTGAATCCCCAGTCATTTCCAGCACCCAAAGAAAATCCCGACAAGGAAGA
ATTTCGCACGATGTGGGTGATTGGGTTAGTGTTTAAAAAAACAGAAAACTCTGAAAACCT
CAGTGTTGATCTCACCTATGATATTCAGTCTTTCACAGATACAGTTTATAGGCAAGCAAT
AAACAGCAAGATGTTTGAGGTGGATATGAAAATTGCTGCAATGCATGTAAAAAGAAAGCA
ACTCCATCAACTACTACCTAATCATGTGCTTCAGAAAAAGAAAAAGCATTCAACAGAAGG
TGTCAAATTGACAGCTCTCAATGACAGCAGCCTCGACTTGTCTATGGACAGTGATAACAG
CATGTCTGTGCCTTCACCTACTAGTGCTACGAAGACCAGTCCATTGAACAGTTCTGGCAG
CTCTCAGGGCAGAAACAGTCCTGCTCCAGCTGTAACAGCAGCATCTGTGACCAACATACA
GGCTACTGAAGTTTCTGTGCCACAAGTAAATTCCAGTGAAAGCTCAGGGGGTACATCGAG
TGAAAGCATTCCTCAAACTGCCACACAACCAGCCATTTCTCCACCACCAAAGCCTACGGT
CTCCAGAGTTGTTTCTTCAACACGTCTGGTAAACCCACCACCTAGATCTTCAGGAAATGC
AGCAACTTCAGGAAATGCAGCAACAAAAATACCTACTCCTATAGTAGGAGTCAAGAGGAC
ATCCTCACCTCATAAAGAAGAGAGTCCCAAGAAAACCAAAACAGAAGAGGATGAAACAAG
TGAAGATGCTAACTGTCTTGCTTTGAGTGGACATGATAAAACAGAAGCAAAGGAACAACT
TGATACAGAGACAAGTACAACTCAATCAGAAACTATTCAGACAGCGGCTTCTCTGTTGGC
CTCTCAGAAAACATCCAGTACAGACCTTTCTGATATCCCTGCTCTCCCTGCAAATCCTAT
TCCTGTTATCAAGAATTCAATAAAACTGAGATTGAATCGGGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT