GPR65 ORF clone pFN21AB7141

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB7141

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Clone info.

Species

human

Clone Name

pFN21AB7141 Sequence (with vector) Restriction Map

Product ID

FHC09579

ORF Sequence

DNA sequence (1011 bp)
Protein sequence (337 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB7141

Symbol

GPR65

Alias : TDAG8, hTDAG8

Description

G protein-coupled receptor 65

Original Clone ID

cp01146

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1011 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 337 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9879.1 3e-87 100.0 GPR65 CCDS12669.1 7.2e-28 41.4 GPR4 CCDS9894.2 9.7e-22 36.7 GPR68 CCDS3169.1 2.4e-16 29.1 P2RY1 CCDS14441.1 9e-16 32.9 LPAR4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR005464 1 18 PR01649 Psychosine receptor IPR000276 16 40 PR00237 GPCR IPR005464 37 50 PR01649 Psychosine receptor IPR000276 49 70 PR00237 GPCR IPR005464 70 92 PR01649 Psychosine receptor IPR000276 94 116 PR00237 GPCR IPR005464 117 129 PR01649 Psychosine receptor IPR000276 130 151 PR00237 GPCR IPR005464 151 170 PR01649 Psychosine receptor IPR005464 171 189 PR01649 Psychosine receptor IPR000276 184 207 PR00237 GPCR IPR005464 209 223 PR01649 Psychosine receptor IPR000276 224 248 PR00237 GPCR IPR005464 264 275 PR01649 Psychosine receptor IPR000276 272 298 PR00237 GPCR IPR005464 294 309 PR01649 Psychosine receptor IPR005464 322 337 PR01649 Psychosine receptor HMMPfam IPR000276 36 290 PF00001 GPCR ProfileScan IPR017452 31 290 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 17 FPIVYIFVIIVSIPANIGSLCVS 39 PRIMARY 23 2 49 LGIYLFSLSLSDLLYALTLPLW 70 SECONDARY 22 3 103 AFLTCIAVDRYLAVVYPLKFFFL 125 PRIMARY 23 4 132 LMVSLSIWILETIFNAVMLWEDE 154 SECONDARY 23 5 183 LNLFRTCTGYAIPLVTILICNR 204 SECONDARY 22 6 229 VSITVTFVLCFTPFHVMLLIRCI 251 PRIMARY 23 7 272 YRITVALTSLNCVADPILYCFVT 294 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003599 337 604620 100.0 100.0 XP_016882097 362 600551 41.4 87.8 XP_016882096 362 600551 41.4 87.8 NP_005273 362 600551 41.4 87.8 NP_001171147 365 601404 36.7 92.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7141 Download

>KIBB7141  1011 bp
ATGAACAGCACATGTATTGAAGAACAGCATGACCTGGATCACTATTTGTTTCCCATTGTT
TACATCTTTGTGATTATAGTCAGCATTCCAGCCAATATTGGATCTCTGTGTGTGTCTTTC
CTGCAAGCAAAGAAGGAAAGTGAACTAGGAATTTACCTCTTCAGTTTGTCACTATCAGAT
TTACTCTATGCATTAACTCTCCCTTTATGGATTGATTATACTTGGAATAAAGACAACTGG
ACTTTCTCTCCTGCCTTGTGCAAAGGGAGTGCTTTTCTCATGTACATGAATTTTTACAGC
AGCACAGCATTCCTCACCTGCATTGCCGTTGATCGGTATTTGGCTGTTGTCTACCCTTTG
AAGTTTTTTTTCCTAAGGACAAGAAGATTTGCACTCATGGTCAGCCTGTCCATCTGGATA
TTGGAAACCATCTTCAATGCTGTCATGTTGTGGGAAGATGAAACAGTTGTTGAATATTGC
GATGCCGAAAAGTCTAATTTTACTTTATGCTATGACAAATACCCTTTAGAGAAATGGCAA
ATCAACCTCAACTTGTTCAGGACGTGTACAGGCTATGCAATACCTTTGGTCACCATCCTG
ATCTGCAACCGGAAAGTCTACCAAGCTGTGCGGCACAATAAAGCCACGGAAAACAAGGAA
AAGAAGAGAATCATAAAACTACTTGTCAGCATCACAGTTACTTTTGTCTTATGCTTTACT
CCCTTTCATGTGATGTTGCTGATTCGCTGCATTTTAGAGCATGCTGTGAACTTCGAAGAC
CACAGCAATTCTGGGAAGCGAACTTACACAATGTATAGAATCACGGTTGCATTAACAAGT
TTAAATTGTGTTGCTGATCCAATTCTGTACTGTTTTGTAACCGAAACAGGAAGATATGAT
ATGTGGAATATATTAAAATTCTGCACTGGGAGGTGTAATACATCACAAAGACAAAGAAAA
CGCATACTTTCTGTGTCTACAAAAGATACTATGGAATTAGAGGTCCTTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB7141 Download

>KIBB7141  337 aa
MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESELGIYLFSLSLSD
LLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIAVDRYLAVVYPL
KFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYCDAEKSNFTLCYDKYPLEKWQ
INLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATENKEKKRIIKLLVSITVTFVLCFT
PFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYD
MWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7141 Download

>pFN21AB7141  5716 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACAGCACATGTATTGAAGAACAGCATGACCT
GGATCACTATTTGTTTCCCATTGTTTACATCTTTGTGATTATAGTCAGCATTCCAGCCAA
TATTGGATCTCTGTGTGTGTCTTTCCTGCAAGCAAAGAAGGAAAGTGAACTAGGAATTTA
CCTCTTCAGTTTGTCACTATCAGATTTACTCTATGCATTAACTCTCCCTTTATGGATTGA
TTATACTTGGAATAAAGACAACTGGACTTTCTCTCCTGCCTTGTGCAAAGGGAGTGCTTT
TCTCATGTACATGAATTTTTACAGCAGCACAGCATTCCTCACCTGCATTGCCGTTGATCG
GTATTTGGCTGTTGTCTACCCTTTGAAGTTTTTTTTCCTAAGGACAAGAAGATTTGCACT
CATGGTCAGCCTGTCCATCTGGATATTGGAAACCATCTTCAATGCTGTCATGTTGTGGGA
AGATGAAACAGTTGTTGAATATTGCGATGCCGAAAAGTCTAATTTTACTTTATGCTATGA
CAAATACCCTTTAGAGAAATGGCAAATCAACCTCAACTTGTTCAGGACGTGTACAGGCTA
TGCAATACCTTTGGTCACCATCCTGATCTGCAACCGGAAAGTCTACCAAGCTGTGCGGCA
CAATAAAGCCACGGAAAACAAGGAAAAGAAGAGAATCATAAAACTACTTGTCAGCATCAC
AGTTACTTTTGTCTTATGCTTTACTCCCTTTCATGTGATGTTGCTGATTCGCTGCATTTT
AGAGCATGCTGTGAACTTCGAAGACCACAGCAATTCTGGGAAGCGAACTTACACAATGTA
TAGAATCACGGTTGCATTAACAAGTTTAAATTGTGTTGCTGATCCAATTCTGTACTGTTT
TGTAACCGAAACAGGAAGATATGATATGTGGAATATATTAAAATTCTGCACTGGGAGGTG
TAATACATCACAAAGACAAAGAAAACGCATACTTTCTGTGTCTACAAAAGATACTATGGA
ATTAGAGGTCCTTGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT