ARFGAP1 ORF clone pFN21AB6625

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6625

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Clone info.

Species

human

Clone Name

pFN21AB6625 Sequence (with vector) Restriction Map

Product ID

FHC03510

Flexi Clone PID

FXC03510

ORF Sequence

DNA sequence (1218 bp)
Protein sequence (406 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527594]

Gene

KIBB6625

Symbol

ARFGAP1

Alias : ARF1GAP, HRIHFB2281

Description

ADP-ribosylation factor GTPase activating protein 1, transcript variant 1

Original Clone ID

cs01225

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1218 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 406 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13515.1 1.4e-152 100.0 ARFGAP1 CCDS13516.1 9.2e-137 97.1 ARFGAP1 CCDS63327.1 1.4e-113 96.4 ARFGAP1 CCDS63326.1 1.9e-101 99.6 ARFGAP1 CCDS63328.1 1e-95 93.3 ARFGAP1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001164 19 38 PR00405 Arf GTPase activating protein IPR001164 38 55 PR00405 Arf GTPase activating protein IPR001164 59 80 PR00405 Arf GTPase activating protein HMMPfam IPR001164 8 115 PF01412 Arf GTPase activating protein HMMSmart IPR001164 7 124 SM00105 Arf GTPase activating protein ProfileScan IPR001164 7 124 PS50115 Arf GTPase activating protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060679 406 608377 100.0 100.0 XP_006723886 404 608377 99.5 100.0 NP_783202 414 608377 97.1 100.0 NP_001268412 361 608377 96.4 86.2 XP_011527203 416 608377 97.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6625 Download

>KIBB6625  1218 bp
ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAAC
GTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGG
ATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGC
TCTGTTACTATGGACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAAT
GCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAG
GAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAA
GGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGG
ACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCG
GACAAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGG
AATCGCTACGTGGGGTTTGGGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTC
AACAACGCCATGTCCTCCCTGTACTCGGGCTGGAGCAGCTTCACCACTGGAGCCAGCCGG
TTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTTGGATCCCAAGCGAGTCAGAAGGCG
TCCGAGCTGGGCCACAGCCTGAACGAGAACGTCCTCAAGCCTGCGCAGGAGAAGGTGAAG
GAGGGAAAGATTTTTGATGATGTCTCCAGTGGGGTCTCTCAGTTGGCGTCCAAGGTCCAG
GGAGTCGGTAGTAAGGGATGGCGGGACGTCACCACCTTTTTTTCGGGGAAAGCAGAGGGC
CCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCAGAACAGCGGTCTGGACCACTTCCAA
AACAGCAACATAGACCAGAGCTTCTGGGAGACCTTTGGAAGTGCTGAGCCCACCAAGACC
CGCAAGTCCCCGAGCAGCGACAGCTGGACGTGCGCGGACACCTCCACCGAGAGGAGGAGC
TCGGACAGCTGGGAGGTGTGGGGCTCGGCCTCCACCAACAGGAACAGCAACAGCGACGGC
GGGGAGGGCGGGGAGGGCACCAAGAAGGCAGTGCCGCCGGCCGTGCCCACTGATGATGGC
TGGGACAACCAGAACTGG

ORF Protein

Cloned ORF protein sequence for pFN21AB6625 Download

>KIBB6625  406 aa
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVR
SVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAE
GREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQG
NRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKA
SELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEG
PLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRS
SDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6625 Download

>pFN21AB6625  5923 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGA
AGTCAGGGTGCAGGATGAGAACAACGTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTG
GGTCAGTGTGACCTACGGCATCTGGATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCT
TGGGGTTCACCTCAGCTTTGTGCGCTCTGTTACTATGGACAAGTGGAAGGACATTGAGCT
TGAGAAGATGAAAGCTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGA
TTACGATCCTTGCTGGTCCTTGCAGGAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAG
GGATAAGGTGGTCGCTCTGGCCGAAGGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCA
GAACTGGACCCCACCTCAGCCCAGGACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCA
GCCGCAGAGTGTGACCGCCTCCTCGGACAAGGCTTTTGAAGACTGGCTGAATGATGACCT
CGGCTCCTATCAAGGGGCCCAGGGGAATCGCTACGTGGGGTTTGGGAACACGCCACCGCC
TCAGAAGAAAGAAGATGACTTCCTCAACAACGCCATGTCCTCCCTGTACTCGGGCTGGAG
CAGCTTCACCACTGGAGCCAGCCGGTTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTT
TGGATCCCAAGCGAGTCAGAAGGCGTCCGAGCTGGGCCACAGCCTGAACGAGAACGTCCT
CAAGCCTGCGCAGGAGAAGGTGAAGGAGGGAAAGATTTTTGATGATGTCTCCAGTGGGGT
CTCTCAGTTGGCGTCCAAGGTCCAGGGAGTCGGTAGTAAGGGATGGCGGGACGTCACCAC
CTTTTTTTCGGGGAAAGCAGAGGGCCCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCA
GAACAGCGGTCTGGACCACTTCCAAAACAGCAACATAGACCAGAGCTTCTGGGAGACCTT
TGGAAGTGCTGAGCCCACCAAGACCCGCAAGTCCCCGAGCAGCGACAGCTGGACGTGCGC
GGACACCTCCACCGAGAGGAGGAGCTCGGACAGCTGGGAGGTGTGGGGCTCGGCCTCCAC
CAACAGGAACAGCAACAGCGACGGCGGGGAGGGCGGGGAGGGCACCAAGAAGGCAGTGCC
GCCGGCCGTGCCCACTGATGATGGCTGGGACAACCAGAACTGGGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT