BSPRY ORF clone pFN21AB6578

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Halotag ORF Clone pFN21AB6578

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Clone info.

Species

human

Clone Name

pFN21AB6578 Sequence (with vector) Restriction Map

Product ID

FHC03504

Flexi Clone PID

FXC03504

ORF Sequence

DNA sequence (1206 bp)
Protein sequence (402 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527588]

Gene

KIBB6578

Symbol

BSPRY

Description

B-box and SPRY domain containing

Original Clone ID

cs01178

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1206 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 402 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43868.1 4.2e-139 99.8 BSPRY CCDS34378.1 2.1e-13 25.1 TRIM39 CCDS32437.1 4.9e-12 24.9 TRIM72 CCDS4677.1 2.4e-11 24.9 TRIM15 CCDS34654.1 5.2e-11 21.4 TRIM50 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003879 228 245 PR01407 Butyrophylin-like IPR003879 245 262 PR01407 Butyrophylin-like IPR003879 268 292 PR01407 Butyrophylin-like IPR003879 298 311 PR01407 Butyrophylin-like IPR003879 345 369 PR01407 Butyrophylin-like IPR003879 375 393 PR01407 Butyrophylin-like HMMPfam IPR000315 20 57 PF00643 Zinc finger IPR003877 284 398 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR006574 229 282 SM00589 SPRY-associated ProfileScan IPR001870 212 402 PS50188 B302/SPRY domain Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6578 Download

>KIBB6578  1206 bp
ATGTCCGCCGAGGGCGCGGAGCCGGGGCCGGGGTCCGGGTCCGGGCCCGGGCCGGGGCCA
CTCTGCCCCGAACACGGCCAGGCTCTGAGCTGGTTCTGCGGCTCCGAGCGACGGCCCGTG
TGCGCCGCCTGCGCGGGGTTGGGCGGTCGCTGCCGGGGGCACCGCATCCGCCGGGCGGAG
GAGCGCGCCGAGGAGCTGCGGAACAAGATTGTGGACCAGTGTGAGAGGCTGCAGTTACAG
AGTGCTGCCATCACCAAGTATGTGGCGGACGTCCTGCCGGGGAAGAATCAAAGAGCAGTG
AGCATGGCCAGTGCAGCGAGGGAACTGGTTATCCAGCGGTTGAGTCTGGTGAGGAGTCTT
TGCGAGAGCGAGGAGCAGCGGTTACTGGAACAGGTGCATGGCGAAGAGGAGCGGGCCCAC
CAGAGCATCCTGACACAGCGGGTGCACTGGGCCGAGGCGCTGCAGAAACTTGACACCATC
CGCACTGGCCTGGTGGGCATGCTTACTCACCTGGATGACCTCCAGCTGATTCAGAAGGAG
CAAGAGATTTTCGAGAGGACCGAAGAAGCAGAGGGCATTTTGGATCCCCAGGAGTCGGAA
ATGTTAAACTTTAATGAGAAGTGCACTCGGAGCCCACTACTGACCCAACTCTGGGCAACG
GCGGTTCTTGGGTCTCTCTCAGGCACAGAGGACATACGGATCGATGAGAGGACAGTCAGC
CCCTTCCTGCAATTGTCAGATGATCGAAAGACCCTGACCTTCAGCACCAAGAAGTCAAAG
GCCTGTGCAGATGGCCCGGAGCGCTTCGACCACTGGCCCAATGCCCTGGCTGCCACCTCC
TTCCAGAATGGGCTCCATGCCTGGATGGTGAATGTCCAGAACAGTTGTGCCTATAAGGTG
GGCGTGGCTTCAGGCCACCTGCCCCGCAAGGGTTCTGGCAGTGACTGCCGTCTGGGCCAC
AATGCCTTCTCCTGGGTCTTCTCTCGCTATGATCAGGAGTTTCGTTTCTCACACAATGGG
CAGCACGAGCCCCTGGGGCTGCTGCGGGGCCCAGCCCAGCTGGGTGTAGTGCTGGACTTG
CAGGTTCAGGAGCTGCTCTTCTATGAGCCAGCCTCCGGCATAGTGCTCTGTGCCCATCAT
GTGTCCTTCCCGGGGCCCCTCTTCCCAGTCTTTGCTGTGGCCGATCAGACCATTTCTATC
GTCCGC

ORF Protein

Cloned ORF protein sequence for pFN21AB6578 Download

>KIBB6578  402 aa
MSAEGAEPGPGSGSGPGPGPLCPEHGQALSWFCGSERRPVCAACAGLGGRCRGHRIRRAE
ERAEELRNKIVDQCERLQLQSAAITKYVADVLPGKNQRAVSMASAARELVIQRLSLVRSL
CESEEQRLLEQVHGEEERAHQSILTQRVHWAEALQKLDTIRTGLVGMLTHLDDLQLIQKE
QEIFERTEEAEGILDPQESEMLNFNEKCTRSPLLTQLWATAVLGSLSGTEDIRIDERTVS
PFLQLSDDRKTLTFSTKKSKACADGPERFDHWPNALAATSFQNGLHAWMVNVQNSCAYKV
GVASGHLPRKGSGSDCRLGHNAFSWVFSRYDQEFRFSHNGQHEPLGLLRGPAQLGVVLDL
QVQELLFYEPASGIVLCAHHVSFPGPLFPVFAVADQTISIVR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6578 Download

>pFN21AB6578  5911 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCGCCGAGGGCGCGGAGCCGGGGCCGGGGTC
CGGGTCCGGGCCCGGGCCGGGGCCACTCTGCCCCGAACACGGCCAGGCTCTGAGCTGGTT
CTGCGGCTCCGAGCGACGGCCCGTGTGCGCCGCCTGCGCGGGGTTGGGCGGTCGCTGCCG
GGGGCACCGCATCCGCCGGGCGGAGGAGCGCGCCGAGGAGCTGCGGAACAAGATTGTGGA
CCAGTGTGAGAGGCTGCAGTTACAGAGTGCTGCCATCACCAAGTATGTGGCGGACGTCCT
GCCGGGGAAGAATCAAAGAGCAGTGAGCATGGCCAGTGCAGCGAGGGAACTGGTTATCCA
GCGGTTGAGTCTGGTGAGGAGTCTTTGCGAGAGCGAGGAGCAGCGGTTACTGGAACAGGT
GCATGGCGAAGAGGAGCGGGCCCACCAGAGCATCCTGACACAGCGGGTGCACTGGGCCGA
GGCGCTGCAGAAACTTGACACCATCCGCACTGGCCTGGTGGGCATGCTTACTCACCTGGA
TGACCTCCAGCTGATTCAGAAGGAGCAAGAGATTTTCGAGAGGACCGAAGAAGCAGAGGG
CATTTTGGATCCCCAGGAGTCGGAAATGTTAAACTTTAATGAGAAGTGCACTCGGAGCCC
ACTACTGACCCAACTCTGGGCAACGGCGGTTCTTGGGTCTCTCTCAGGCACAGAGGACAT
ACGGATCGATGAGAGGACAGTCAGCCCCTTCCTGCAATTGTCAGATGATCGAAAGACCCT
GACCTTCAGCACCAAGAAGTCAAAGGCCTGTGCAGATGGCCCGGAGCGCTTCGACCACTG
GCCCAATGCCCTGGCTGCCACCTCCTTCCAGAATGGGCTCCATGCCTGGATGGTGAATGT
CCAGAACAGTTGTGCCTATAAGGTGGGCGTGGCTTCAGGCCACCTGCCCCGCAAGGGTTC
TGGCAGTGACTGCCGTCTGGGCCACAATGCCTTCTCCTGGGTCTTCTCTCGCTATGATCA
GGAGTTTCGTTTCTCACACAATGGGCAGCACGAGCCCCTGGGGCTGCTGCGGGGCCCAGC
CCAGCTGGGTGTAGTGCTGGACTTGCAGGTTCAGGAGCTGCTCTTCTATGAGCCAGCCTC
CGGCATAGTGCTCTGTGCCCATCATGTGTCCTTCCCGGGGCCCCTCTTCCCAGTCTTTGC
TGTGGCCGATCAGACCATTTCTATCGTCCGCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT