SYT5 ORF clone pFN21AB6518

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Halotag ORF Clone pFN21AB6518

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Clone info.

Species

human

Clone Name

pFN21AB6518 Sequence (with vector) Restriction Map

Product ID

FHC10569

Flexi Clone PID

FXC10569

ORF Sequence

DNA sequence (1158 bp)
Protein sequence (386 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528635]

Gene

KIBB6518

Symbol

SYT5

Description

synaptotagmin V, transcript variant 1

Original Clone ID

cs01118

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1158 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 386 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12919.1 1.9e-123 100.0 SYT5 CCDS74455.1 3.4e-93 83.8 SYT5 CCDS1427.1 1.3e-73 62.5 SYT2 CCDS76582.1 1.4e-72 59.9 SYT1 CCDS9017.1 2.7e-72 59.8 SYT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001565 112 127 PR00399 Synaptotagmin IPR001565 127 140 PR00399 Synaptotagmin IPR020477 140 152 PR00360 C2 region IPR020477 167 180 PR00360 C2 region IPR001565 182 197 PR00399 Synaptotagmin IPR020477 191 199 PR00360 C2 region IPR001565 202 212 PR00399 Synaptotagmin HMMPfam IPR000008 125 210 PF00168 C2 calcium-dependent membrane targeting IPR000008 256 343 PF00168 C2 calcium-dependent membrane targeting HMMSmart IPR000008 124 226 SM00239 C2 calcium-dependent membrane targeting IPR000008 255 369 SM00239 C2 calcium-dependent membrane targeting ProfileScan IPR018029 122 211 PS50004 C2 membrane targeting protein IPR018029 253 344 PS50004 C2 membrane targeting protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 29 PWALATIVLVSGLLIFSCCFCLY 51 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016882665 386 600782 100.0 100.0 XP_016882664 386 600782 100.0 100.0 XP_006723402 386 600782 100.0 100.0 NP_003171 386 600782 100.0 100.0 XP_006723403 385 600782 99.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6518 Download

>KIBB6518  1158 bp
ATGTTCCCGGAGCCCCCAACCCCGGGGCCTCCATCGCCCGACACGCCTCCCGACTCCAGT
CGCATCAGCCACGGCCCAGTGCCCCCCTGGGCCCTGGCCACCATCGTGCTGGTCTCAGGC
CTCCTCATCTTCAGCTGCTGTTTCTGTCTCTACCGGAAGAGCTGTCGGAGGCGGACAGGC
AAGAAGAGCCAGGCCCAAGCCCAGGTCCACCTTCAGGAAGTGAAGGGGCTGGGCCAGAGT
TACATAGACAAGGTGCAGCCAGAAGTAGAGGAGCTGGAGCCAGCACCATCCGGGCCAGGG
CAGCAGGTGGCAGACAAGCATGAGCTAGGACGACTGCAGTACTCCCTGGATTATGACTTC
CAGAGTGGCCAGCTGCTGGTGGGCATTCTGCAAGCAATGGGATTGGCAGCCTTGGATCTT
GGTGGCTCCTCGGACCCCTATGTGCGGGTCTACCTGCTGCCGGACAAACGGAGGCGGTAC
GAGACCAAGGTGCATCGGCAGACGCTGAACCCTCACTTTGGGGAGACCTTCGCCTTCAAG
GTCCCCTACGTGGAGCTGGGGGGCAGGGTGCTGGTCATGGCGGTGTACGACTTCGACCGC
TTCTCTCGCAATGACGCCATCGGGGAGGTGCGGGTCCCTATGAGCTCCGTGGACCTGGGG
CGGCCAGTGCAGGCCTGGCGGGAGCTGCAGGCGGCTCCGCGGGAGGAGCAGGAGAAGCTT
GGGGACATCTGCTTCTCCCTCCGCTATGTCCCCACGGCCGGGAAGCTCACCGTCATCGTC
CTGGAGGCTAAAAACCTGAAGAAGATGGACGTAGGAGGACTGTCAGATCCATACGTCAAG
GTCCACCTGCTGCAGGGCGGCAAAAAGGTGCGGAAGAAGAAAACCACCATCAAGAAGAAC
ACTCTGAACCCCTATTACAACGAAGCTTTCAGCTTCGAGGTGCCCTGTGACCAAGTCCAG
AAGGTGCAGGTGGAGCTGACCGTGCTGGACTACGACAAGCTGGGCAAGAACGAGGCCATC
GGGAGGGTGGCCGTGGGGGCGGCCGCCGGCGGGGCTGGCCTGCGGCACTGGGCGGACATG
CTGGCCAACCCGCGGCGGCCCATTGCCCAGTGGCACTCGCTGCGGCCCCCGGACCGAGTG
AGGCTGCTGCCTGCGCCC

ORF Protein

Cloned ORF protein sequence for pFN21AB6518 Download

>KIBB6518  386 aa
MFPEPPTPGPPSPDTPPDSSRISHGPVPPWALATIVLVSGLLIFSCCFCLYRKSCRRRTG
KKSQAQAQVHLQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQVADKHELGRLQYSLDYDF
QSGQLLVGILQAMGLAALDLGGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFK
VPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVDLGRPVQAWRELQAAPREEQEKL
GDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVKVHLLQGGKKVRKKKTTIKKN
TLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAGGAGLRHWADM
LANPRRPIAQWHSLRPPDRVRLLPAP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6518 Download

>pFN21AB6518  5863 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCCCGGAGCCCCCAACCCCGGGGCCTCCATC
GCCCGACACGCCTCCCGACTCCAGTCGCATCAGCCACGGCCCAGTGCCCCCCTGGGCCCT
GGCCACCATCGTGCTGGTCTCAGGCCTCCTCATCTTCAGCTGCTGTTTCTGTCTCTACCG
GAAGAGCTGTCGGAGGCGGACAGGCAAGAAGAGCCAGGCCCAAGCCCAGGTCCACCTTCA
GGAAGTGAAGGGGCTGGGCCAGAGTTACATAGACAAGGTGCAGCCAGAAGTAGAGGAGCT
GGAGCCAGCACCATCCGGGCCAGGGCAGCAGGTGGCAGACAAGCATGAGCTAGGACGACT
GCAGTACTCCCTGGATTATGACTTCCAGAGTGGCCAGCTGCTGGTGGGCATTCTGCAAGC
AATGGGATTGGCAGCCTTGGATCTTGGTGGCTCCTCGGACCCCTATGTGCGGGTCTACCT
GCTGCCGGACAAACGGAGGCGGTACGAGACCAAGGTGCATCGGCAGACGCTGAACCCTCA
CTTTGGGGAGACCTTCGCCTTCAAGGTCCCCTACGTGGAGCTGGGGGGCAGGGTGCTGGT
CATGGCGGTGTACGACTTCGACCGCTTCTCTCGCAATGACGCCATCGGGGAGGTGCGGGT
CCCTATGAGCTCCGTGGACCTGGGGCGGCCAGTGCAGGCCTGGCGGGAGCTGCAGGCGGC
TCCGCGGGAGGAGCAGGAGAAGCTTGGGGACATCTGCTTCTCCCTCCGCTATGTCCCCAC
GGCCGGGAAGCTCACCGTCATCGTCCTGGAGGCTAAAAACCTGAAGAAGATGGACGTAGG
AGGACTGTCAGATCCATACGTCAAGGTCCACCTGCTGCAGGGCGGCAAAAAGGTGCGGAA
GAAGAAAACCACCATCAAGAAGAACACTCTGAACCCCTATTACAACGAAGCTTTCAGCTT
CGAGGTGCCCTGTGACCAAGTCCAGAAGGTGCAGGTGGAGCTGACCGTGCTGGACTACGA
CAAGCTGGGCAAGAACGAGGCCATCGGGAGGGTGGCCGTGGGGGCGGCCGCCGGCGGGGC
TGGCCTGCGGCACTGGGCGGACATGCTGGCCAACCCGCGGCGGCCCATTGCCCAGTGGCA
CTCGCTGCGGCCCCCGGACCGAGTGAGGCTGCTGCCTGCGCCCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT