SH3GL3 ORF clone pFN21AB6476

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Halotag ORF Clone pFN21AB6476

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Clone info.

Species

human

Clone Name

pFN21AB6476 Sequence (with vector) Restriction Map

Product ID

FHC10579

Flexi Clone PID

FXC10579

ORF Sequence

DNA sequence (1065 bp)
Protein sequence (355 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528645]

Gene

KIBB6476

Symbol

SH3GL3

Alias : CNSA3, EEN-B2, HsT19371, SH3D2C, SH3P13

Description

SH3-domain GRB2-like 3, transcript variant EEN-B2-L3

Original Clone ID

cs01076

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1065 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 355 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS73772.1 1.1e-101 100.0 SH3GL3 CCDS10325.2 2.3e-94 99.4 SH3GL3 CCDS6483.1 2.5e-65 67.1 SH3GL2 CCDS32874.1 2.9e-49 64.7 SH3GL1 CCDS56076.1 2.2e-27 54.7 SH3GL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001452 296 306 PR00452 Src homology-3 domain IPR001452 310 325 PR00452 Src homology-3 domain IPR001452 327 336 PR00452 Src homology-3 domain IPR001452 338 350 PR00452 Src homology-3 domain HMMPfam IPR004148 22 249 PF03114 BAR IPR001452 300 343 PF00018 Src homology-3 domain HMMSmart IPR004148 18 250 SM00721 BAR IPR001452 296 351 SM00326 Src homology-3 domain ProfileScan IPR004148 26 257 PS51021 BAR IPR001452 293 352 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011520191 355 603362 100.0 100.0 NP_001288038 355 603362 100.0 100.0 NP_001311112 355 603362 100.0 100.0 NP_003018 347 603362 99.4 94.4 NP_001311113 312 603362 100.0 87.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6476 Download

>KIBB6476  1065 bp
ATGGATGGAATCTTTGCTGGGATAATATGCAATCAAGCTAATAGGTGCCTTACCTGGACC
TCCCAACAGCTATTTAGTGAAAAAATAAGTGGTGCTGAAGGAACTAAACTAGACGATGAA
TTTCTTGACATGGAAAGGAAAATAGATGTTACCAATAAAGTTGTTGCAGAAATTCTTTCA
AAAACCACTGAATATCTTCAGCCAAATCCAGCATACAGAGCTAAGCTAGGAATGCTGAAC
ACTGTGTCGAAGATCCGAGGGCAGGTGAAGACCACAGGATACCCGCAGACGGAAGGCTTG
CTGGGGGACTGTATGCTGAAATACGGGAAGGAGCTCGGGGAAGACTCCACCTTTGGCAAT
GCATTGATAGAAGTTGGTGAATCCATGAAGCTAATGGCTGAGGTGAAAGACTCTCTTGAT
ATTAATGTAAAGCAAACTTTTATTGATCCACTTCAGTTACTACAAGATAAAGATTTAAAA
GAGATCGGGCATCACCTGAAAAAGCTGGAAGGCCGCCGCCTGGATTACGATTATAAAAAG
AAACGAGTAGGTAAGATACCAGACGAAGAAGTCAGACAAGCGGTAGAAAAATTTGAAGAG
TCAAAGGAGTTGGCTGAAAGAAGCATGTTTAACTTTTTAGAAAATGATGTAGAACAAGTC
AGCCAGTTGGCTGTGTTCATAGAGGCAGCATTAGACTATCACAGACAGTCCACAGAGATT
CTGCAGGAGCTGCAGAGCAAGCTACAGATGCGAATATCAGCTGCATCCAGTGTCCCCAGA
CGAGAATACAAGCCAAGGCCTGTGAAAAGGAGTTCTAGTGAGCTCAATGGAGTTTCCACC
ACCTCTGTAGTGAAGACGACAGGTTCTAACATTCCCATGGACCAGCCCTGCTGTCGTGGT
CTCTATGACTTTGAGCCAGAAAACCAAGGAGAATTAGGATTTAAAGAAGGGGACATCATT
ACATTAACCAATCAAATAGATGAAAACTGGTATGAAGGAATGATACACGGAGAATCGGGA
TTCTTCCCCATTAATTACGTGGAAGTGATCGTGCCTTTACCTCAG

ORF Protein

Cloned ORF protein sequence for pFN21AB6476 Download

>KIBB6476  355 aa
MDGIFAGIICNQANRCLTWTSQQLFSEKISGAEGTKLDDEFLDMERKIDVTNKVVAEILS
KTTEYLQPNPAYRAKLGMLNTVSKIRGQVKTTGYPQTEGLLGDCMLKYGKELGEDSTFGN
ALIEVGESMKLMAEVKDSLDINVKQTFIDPLQLLQDKDLKEIGHHLKKLEGRRLDYDYKK
KRVGKIPDEEVRQAVEKFEESKELAERSMFNFLENDVEQVSQLAVFIEAALDYHRQSTEI
LQELQSKLQMRISAASSVPRREYKPRPVKRSSSELNGVSTTSVVKTTGSNIPMDQPCCRG
LYDFEPENQGELGFKEGDIITLTNQIDENWYEGMIHGESGFFPINYVEVIVPLPQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6476 Download

>pFN21AB6476  5770 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATGGAATCTTTGCTGGGATAATATGCAATCA
AGCTAATAGGTGCCTTACCTGGACCTCCCAACAGCTATTTAGTGAAAAAATAAGTGGTGC
TGAAGGAACTAAACTAGACGATGAATTTCTTGACATGGAAAGGAAAATAGATGTTACCAA
TAAAGTTGTTGCAGAAATTCTTTCAAAAACCACTGAATATCTTCAGCCAAATCCAGCATA
CAGAGCTAAGCTAGGAATGCTGAACACTGTGTCGAAGATCCGAGGGCAGGTGAAGACCAC
AGGATACCCGCAGACGGAAGGCTTGCTGGGGGACTGTATGCTGAAATACGGGAAGGAGCT
CGGGGAAGACTCCACCTTTGGCAATGCATTGATAGAAGTTGGTGAATCCATGAAGCTAAT
GGCTGAGGTGAAAGACTCTCTTGATATTAATGTAAAGCAAACTTTTATTGATCCACTTCA
GTTACTACAAGATAAAGATTTAAAAGAGATCGGGCATCACCTGAAAAAGCTGGAAGGCCG
CCGCCTGGATTACGATTATAAAAAGAAACGAGTAGGTAAGATACCAGACGAAGAAGTCAG
ACAAGCGGTAGAAAAATTTGAAGAGTCAAAGGAGTTGGCTGAAAGAAGCATGTTTAACTT
TTTAGAAAATGATGTAGAACAAGTCAGCCAGTTGGCTGTGTTCATAGAGGCAGCATTAGA
CTATCACAGACAGTCCACAGAGATTCTGCAGGAGCTGCAGAGCAAGCTACAGATGCGAAT
ATCAGCTGCATCCAGTGTCCCCAGACGAGAATACAAGCCAAGGCCTGTGAAAAGGAGTTC
TAGTGAGCTCAATGGAGTTTCCACCACCTCTGTAGTGAAGACGACAGGTTCTAACATTCC
CATGGACCAGCCCTGCTGTCGTGGTCTCTATGACTTTGAGCCAGAAAACCAAGGAGAATT
AGGATTTAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAAACTGGTATGA
AGGAATGATACACGGAGAATCGGGATTCTTCCCCATTAATTACGTGGAAGTGATCGTGCC
TTTACCTCAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT