GNB4 ORF clone pFN21AB6424

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Halotag ORF Clone pFN21AB6424

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Clone info.

Species

human

Clone Name

pFN21AB6424 Sequence (with vector) Restriction Map

Product ID

FHC06872

Flexi Clone PID

FXC06872

ORF Sequence

DNA sequence (1020 bp)
Protein sequence (340 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587413]

Gene

KIBB6424

Symbol

GNB4

Alias : CMTD1F

Description

guanine nucleotide binding protein (G protein), beta polypeptide 4

Original Clone ID

cs01024

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1020 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 340 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3230.1 1.5e-112 100.0 GNB4 CCDS34.1 5.9e-104 90.9 GNB1 CCDS5703.1 6.7e-104 90.0 GNB2 CCDS8564.1 3.4e-94 79.7 GNB3 CCDS73427.1 6.2e-93 79.4 GNB3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001632 51 67 PR00319 G-protein IPR001632 70 84 PR00319 G-protein IPR020472 70 84 PR00320 G-protein beta WD-40 repeat IPR001632 89 104 PR00319 G-protein IPR001632 107 124 PR00319 G-protein IPR020472 157 171 PR00320 G-protein beta WD-40 repeat IPR020472 199 213 PR00320 G-protein beta WD-40 repeat HMMPfam IPR019781 49 83 PF00400 WD40 repeat IPR019781 97 125 PF00400 WD40 repeat IPR019781 137 170 PF00400 WD40 repeat IPR019781 176 212 PF00400 WD40 repeat IPR019781 217 254 PF00400 WD40 repeat IPR019781 272 298 PF00400 WD40 repeat IPR019781 306 340 PF00400 WD40 repeat HMMSmart IPR001680 44 83 SM00320 WD40 repeat IPR001680 86 125 SM00320 WD40 repeat IPR001680 132 170 SM00320 WD40 repeat IPR001680 173 212 SM00320 WD40 repeat IPR001680 215 254 SM00320 WD40 repeat IPR001680 257 298 SM00320 WD40 repeat IPR001680 301 340 SM00320 WD40 repeat ProfileScan IPR019782 51 92 PS50082 WD40 repeat 2 IPR017986 51 340 PS50294 WD40-repeat-containing domain IPR019782 139 179 PS50082 WD40 repeat 2 IPR019782 180 221 PS50082 WD40 repeat 2 IPR019782 222 263 PS50082 WD40 repeat 2 IPR019782 273 307 PS50082 WD40 repeat 2 IPR019782 308 340 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006713784 340 610863,615185 100.0 100.0 NP_067642 340 610863,615185 100.0 100.0 XP_005247749 340 610863,615185 100.0 100.0 XP_016856550 340 139380,613065,616973 90.9 100.0 XP_016856549 340 139380,613065,616973 90.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6424 Download

>KIBB6424  1020 bp
ATGAGCGAACTGGAACAGTTGAGGCAAGAAGCAGAACAACTGCGGAATCAGATTCAGGAT
GCTCGGAAAGCATGTAATGATGCAACGCTTGTTCAGATTACATCAAATATGGACTCTGTG
GGTCGAATACAAATGCGAACAAGACGTACACTGAGGGGCCACCTAGCTAAAATCTATGCT
ATGCATTGGGGATACGATTCCAGGCTGCTAGTCAGTGCTTCTCAAGATGGAAAATTAATT
ATTTGGGATAGCTATACAACAAATAAGATGCATGCTATTCCTTTGAGGTCCTCCTGGGTG
ATGACCTGTGCTTATGCTCCCTCTGGTAATTATGTTGCCTGTGGAGGCTTGGACAACATC
TGCTCTATATATAACTTAAAGACCAGAGAGGGAAATGTGAGAGTAAGCCGAGAGTTGCCA
GGTCACACAGGGTACTTGTCCTGCTGTCGTTTTTTAGATGACAGCCAAATTGTTACAAGT
TCAGGAGATACAACTTGTGCTTTATGGGACATCGAAACTGCCCAGCAGACCACCACATTC
ACTGGGCATTCTGGAGATGTGATGAGTCTTTCTTTGAGTCCTGACATGAGGACTTTTGTT
TCTGGTGCTTGTGATGCCTCTTCCAAATTATGGGATATTCGAGATGGAATGTGTAGACAG
TCTTTCACGGGACATGTCTCAGATATCAATGCTGTCAGTTTTTTCCCAAATGGATATGCC
TTCGCCACTGGCTCTGATGATGCCACTTGCCGGCTCTTTGACCTTCGTGCAGATCAAGAG
TTATTATTGTATTCTCATGACAATATCATCTGTGGAATCACTTCTGTAGCCTTCTCAAAA
AGTGGGCGTCTCTTGTTGGCTGGTTACGATGACTTTAATTGTAATGTATGGGACACGCTA
AAAGGAGATCGTGCAGGTGTCCTTGCTGGTCATGACAACCGTGTGAGCTGCTTAGGTGTA
ACTGATGATGGCATGGCTGTGGCAACAGGCTCTTGGGACAGTTTTCTTAGAATCTGGAAT

ORF Protein

Cloned ORF protein sequence for pFN21AB6424 Download

>KIBB6424  340 aa
MSELEQLRQEAEQLRNQIQDARKACNDATLVQITSNMDSVGRIQMRTRRTLRGHLAKIYA
MHWGYDSRLLVSASQDGKLIIWDSYTTNKMHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELPGHTGYLSCCRFLDDSQIVTSSGDTTCALWDIETAQQTTTF
TGHSGDVMSLSLSPDMRTFVSGACDASSKLWDIRDGMCRQSFTGHVSDINAVSFFPNGYA
FATGSDDATCRLFDLRADQELLLYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTL
KGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLRIWN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6424 Download

>pFN21AB6424  5725 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCGAACTGGAACAGTTGAGGCAAGAAGCAGA
ACAACTGCGGAATCAGATTCAGGATGCTCGGAAAGCATGTAATGATGCAACGCTTGTTCA
GATTACATCAAATATGGACTCTGTGGGTCGAATACAAATGCGAACAAGACGTACACTGAG
GGGCCACCTAGCTAAAATCTATGCTATGCATTGGGGATACGATTCCAGGCTGCTAGTCAG
TGCTTCTCAAGATGGAAAATTAATTATTTGGGATAGCTATACAACAAATAAGATGCATGC
TATTCCTTTGAGGTCCTCCTGGGTGATGACCTGTGCTTATGCTCCCTCTGGTAATTATGT
TGCCTGTGGAGGCTTGGACAACATCTGCTCTATATATAACTTAAAGACCAGAGAGGGAAA
TGTGAGAGTAAGCCGAGAGTTGCCAGGTCACACAGGGTACTTGTCCTGCTGTCGTTTTTT
AGATGACAGCCAAATTGTTACAAGTTCAGGAGATACAACTTGTGCTTTATGGGACATCGA
AACTGCCCAGCAGACCACCACATTCACTGGGCATTCTGGAGATGTGATGAGTCTTTCTTT
GAGTCCTGACATGAGGACTTTTGTTTCTGGTGCTTGTGATGCCTCTTCCAAATTATGGGA
TATTCGAGATGGAATGTGTAGACAGTCTTTCACGGGACATGTCTCAGATATCAATGCTGT
CAGTTTTTTCCCAAATGGATATGCCTTCGCCACTGGCTCTGATGATGCCACTTGCCGGCT
CTTTGACCTTCGTGCAGATCAAGAGTTATTATTGTATTCTCATGACAATATCATCTGTGG
AATCACTTCTGTAGCCTTCTCAAAAAGTGGGCGTCTCTTGTTGGCTGGTTACGATGACTT
TAATTGTAATGTATGGGACACGCTAAAAGGAGATCGTGCAGGTGTCCTTGCTGGTCATGA
CAACCGTGTGAGCTGCTTAGGTGTAACTGATGATGGCATGGCTGTGGCAACAGGCTCTTG
GGACAGTTTTCTTAGAATCTGGAATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT