REM1 ORF clone pFN21AB6368

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Halotag ORF Clone pFN21AB6368

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Clone info.

Species

human

Clone Name

pFN21AB6368 Sequence (with vector) Restriction Map

Product ID

FHC10553

Flexi Clone PID

FXC10553

ORF Sequence

DNA sequence (894 bp)
Protein sequence (298 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528621]

Gene

KIBB6368

Symbol

REM1

Alias : GD:REM, GES

Description

RAS (RAD and GEM)-like GTP-binding 1

Original Clone ID

cs00968

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 894 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 298 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13181.1 2.4e-88 100.0 REM1 CCDS6261.1 1.2e-36 52.1 GEM CCDS10824.1 1.7e-36 51.4 RRAD CCDS45082.1 3.3e-26 42.6 REM2 CCDS11921.1 5.9e-14 36.2 RIT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 81 102 PR00449 Ras GTPase IPR001806 121 143 PR00449 Ras GTPase IPR001806 186 199 PR00449 Ras GTPase IPR001806 221 243 PR00449 Ras GTPase HMMPfam IPR013753 82 243 PF00071 Ras HMMSmart IPR003577 78 246 SM00173 Ras small GTPase IPR003579 81 246 SM00175 Ras small GTPase IPR003578 83 246 SM00174 Small GTPase HMMTigr IPR005225 81 238 TIGR00231 Small GTP-binding protein domain ProfileScan IPR020849 71 298 PS51421 Ras small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_054731 298 610388 100.0 100.0 XP_011527097 304 610388 98.0 100.0 XP_016883322 306 610388 97.4 100.0 XP_005260461 306 610388 97.4 100.0 NP_859053 296 600164 52.1 91.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6368 Download

>KIBB6368  894 bp
ATGACACTCAACACCGAGCAGGAAGCAAAGACCCCTCTGCACCGGCGAGCCAGCACCCCA
CTGCCCCTGTCCCCACGGGGCCACCAGCCTGGCCGCCTGAGCACAGTGCCTTCCACTCAA
TCCCAGCATCCCCGGCTGGGCCAATCAGCCTCCCTCAACCCTCCCACCCAGAAACCTTCA
CCTGCCCCAGATGATTGGTCTTCTGAATCCAGCGACTCTGAAGGCTCCTGGGAGGCTCTC
TACCGTGTGGTGCTACTTGGAGATCCTGGAGTGGGGAAGACCAGCTTGGCCAGCCTCTTT
GCAGGGAAGCAAGAGAGGGACCTCCATGAACAGCTGGGAGAAGATGTATATGAGAGGACC
CTCACGGTGGATGGAGAAGACACCACACTGGTGGTCGTGGACACCTGGGAGGCCGAGAAA
CTGGATAAAAGCTGGAGCCAGGAGTCATGCCTGCAGGGGGGCAGTGCCTATGTCATCGTA
TACTCCATCGCAGACCGAGGCAGCTTTGAGAGTGCCTCTGAGCTCCGCATCCAGCTGCGG
CGCACACATCAGGCAGACCATGTGCCCATCATCCTCGTGGGCAACAAGGCAGACTTGGCC
CGCTGCCGAGAAGTCTCTGTGGAAGAGGGCCGCGCCTGCGCTGTGGTGTTCGACTGTAAA
TTCATCGAGACATCCGCCACGCTGCAGCACAATGTGGCCGAGCTCTTCGAGGGCGTGGTG
CGCCAACTGCGCTTGCGCCGCCGGGACAGTGCGGCCAAGGAACCCCCAGCACCCCGACGG
CCGGCCAGCCTAGCCCAGCGCGCTCGTCGCTTCCTGGCACGCCTGACAGCCCGCAGCGCA
CGCCGCCGGGCACTCAAGGCCCGCTCCAAGTCCTGCCACAATCTGGCCGTGCTC

ORF Protein

Cloned ORF protein sequence for pFN21AB6368 Download

>KIBB6368  298 aa
MTLNTEQEAKTPLHRRASTPLPLSPRGHQPGRLSTVPSTQSQHPRLGQSASLNPPTQKPS
PAPDDWSSESSDSEGSWEALYRVVLLGDPGVGKTSLASLFAGKQERDLHEQLGEDVYERT
LTVDGEDTTLVVVDTWEAEKLDKSWSQESCLQGGSAYVIVYSIADRGSFESASELRIQLR
RTHQADHVPIILVGNKADLARCREVSVEEGRACAVVFDCKFIETSATLQHNVAELFEGVV
RQLRLRRRDSAAKEPPAPRRPASLAQRARRFLARLTARSARRRALKARSKSCHNLAVL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6368 Download

>pFN21AB6368  5599 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACACTCAACACCGAGCAGGAAGCAAAGACCCC
TCTGCACCGGCGAGCCAGCACCCCACTGCCCCTGTCCCCACGGGGCCACCAGCCTGGCCG
CCTGAGCACAGTGCCTTCCACTCAATCCCAGCATCCCCGGCTGGGCCAATCAGCCTCCCT
CAACCCTCCCACCCAGAAACCTTCACCTGCCCCAGATGATTGGTCTTCTGAATCCAGCGA
CTCTGAAGGCTCCTGGGAGGCTCTCTACCGTGTGGTGCTACTTGGAGATCCTGGAGTGGG
GAAGACCAGCTTGGCCAGCCTCTTTGCAGGGAAGCAAGAGAGGGACCTCCATGAACAGCT
GGGAGAAGATGTATATGAGAGGACCCTCACGGTGGATGGAGAAGACACCACACTGGTGGT
CGTGGACACCTGGGAGGCCGAGAAACTGGATAAAAGCTGGAGCCAGGAGTCATGCCTGCA
GGGGGGCAGTGCCTATGTCATCGTATACTCCATCGCAGACCGAGGCAGCTTTGAGAGTGC
CTCTGAGCTCCGCATCCAGCTGCGGCGCACACATCAGGCAGACCATGTGCCCATCATCCT
CGTGGGCAACAAGGCAGACTTGGCCCGCTGCCGAGAAGTCTCTGTGGAAGAGGGCCGCGC
CTGCGCTGTGGTGTTCGACTGTAAATTCATCGAGACATCCGCCACGCTGCAGCACAATGT
GGCCGAGCTCTTCGAGGGCGTGGTGCGCCAACTGCGCTTGCGCCGCCGGGACAGTGCGGC
CAAGGAACCCCCAGCACCCCGACGGCCGGCCAGCCTAGCCCAGCGCGCTCGTCGCTTCCT
GGCACGCCTGACAGCCCGCAGCGCACGCCGCCGGGCACTCAAGGCCCGCTCCAAGTCCTG
CCACAATCTGGCCGTGCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT