SOX30 ORF clone pFN21AB6245

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6245

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Clone info.

Species

human

Clone Name

pFN21AB6245 Sequence (with vector) Restriction Map

Product ID

FHC03612

Flexi Clone PID

FXC03612

ORF Sequence

DNA sequence (2259 bp)
Protein sequence (753 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527685]

Gene

KIBB6245

Symbol

SOX30

Description

SRY (sex determining region Y)-box 30, transcript variant 1

Original Clone ID

cs00845

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2259 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 753 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4339.1 2.9e-160 100.0 SOX30 CCDS4340.1 4.6e-95 100.0 SOX30 CCDS78080.1 3e-91 100.0 SOX30 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000910 337 404 PF00505 High mobility group HMMSmart IPR000910 336 406 SM00398 High mobility group ProfileScan IPR000910 337 405 PS50118 High mobility group
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_848511 753 606698 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6245 Download

>KIBB6245  2259 bp
ATGGAGAGAGCCAGACCCGAGCCGCCGCCTCAGCCGCGCCCGTTGCGTCCCGCTCCGCCC
CCGCTGCCGGTCGAGGGCACCTCCTTTTGGGCAGCAGCCATGGAGCCCCCTCCGTCGTCT
CCCACACTGAGCGCGGCAGCCAGTGCGACCTTGGCCTCGTCGTGCGGGGAGGCAGTGGCG
TCCGGCTTACAGCCCGCGGTGCGGCGGCTGCTGCAGGTGAAGCCAGAGCAGGTGTTGCTG
CTACCACAGCCTCAGGCCCAGAACGAGGAAGCCGCTGCCTCGTCCGCGCAGGCGCGGCTG
TTGCAGTTCAGGCCCGACCTGCGGCTCCTGCAGCCGCCGACAGCGTCAGACGGCGCCACC
TCCAGGCCCGAGTTGCACCCGGTGCAGCCCCTGGCGCTGCATGTCAAGGCCAAGAAGCAG
AAGCTGGGGCCCAGCCTGGATCAGTCAGTGGGGCCTCGAGGGGCCGTCGAAACCGGTCCT
AGAGCCTCCAGGGTGGTCAAGTTGGAAGGCCCCGGGCCGGCCCTCGGCTACTTCCGAGGG
GACGAGAAGGGCAAGCTGGAGGCGGAGGAGGTCATGAGAGACTCGATGCAAGGCGGGGCA
GGCAAAAGCCCGGCAGCCATCCGAGAAGGTGTGATCAAAACGGAGGAACCCGAGAGACTC
CTCGAGGACTGCAGGCTCGGCGCGGAGCCCGCGTCCAATGGCCTGGTTCATGGCAGCGCG
GAGGTCATCTTGGCCCCAACGTCCGGTGCCTTTGGGCCGCACCAGCAAGACCTTAGGATC
CCTTTGACGCTCCACACGGTCCCCCCTGGGGCCCGGATCCAGTTTCAGGGAGCTCCGCCT
TCAGAGCTGATAAGATTGACCAAGGTCCCCCTGACACCAGTGCCTACTAAAATGCAGTCC
CTACTGGAGCCTTCTGTAAAAATTGAAACCAAAGATGTCCCGCTCACCGTGTTGCCCTCA
GATGCAGGCATACCAGATACTCCCTTCAGTAAGGACAGAAATGGTCATGTGAAGCGACCC
ATGAACGCATTTATGGTTTGGGCAAGGATCCACCGACCAGCACTAGCCAAAGCTAACCCA
GCAGCCAACAATGCAGAAATCAGTGTCCAGCTTGGGTTAGAGTGGAACAAACTTAGTGAA
GAACAAAAGAAACCCTATTACGATGAAGCACAAAAGATTAAGGAAAAGCACAGAGAGGAA
TTTCCTGGTTGGGTTTATCAGCCTCGTCCAGGGAAGCGAAAACGATTCCCTCTAAGTGTT
TCCAATGTATTTTCTGGTACCACACAGAATATCATCTCTACAAATCCTACAACAGTTTAT
CCTTACCGCTCACCTACGTACTCTGTGGTAATTCCCAGCCTACAGAATCCCATCACTCAT
CCAGTTGGTGAAACCTCACCTGCTATCCAGCTGCCCACACCTGCAGTCCAGAGCCCAAGC
CCTGTCACACTTTTCCAGCCCAGCGTCTCCAGTGCTGCTCAGGTGGCTGTCCAGGATCCA
AGTCTACCTGTCTATCCAGCACTCCCACCCCAACGCTTTACTGGGCCTTCCCAAACAGAC
ACTCATCAGCTGCATTCTGAAGCCACTCACACTGTGAAGCAACCCACTCCTGTCTCTCTA
GAGAGCGCCAACAGGATTTCAAGTAGTGCAAGTACTGCCCATGCCAGATTTGCAACTTCG
ACCATCCAACCTCCTAGGGAGTATTCCAGCGTTTCCCCTTGTCCCAGAAGTGCTCCAATC
CCCCAGGCTTCTCCCATTCCACACCCACATGTCTACCAGCCCCCTCCCCTTGGCCATCCA
GCCACACTGTTCGGGACACCACCAAGATTCTCTTTTCATCACCCTTACTTCCTACCCGGA
CCTCACTACTTCCCATCAAGTACATGCCCTTACAGTCGGCCTCCCTTTGGCTATGGAAAT
TTTCCGAGTTCAATGCCAGAATGCCTTAGTTATTATGAAGACAGGTACCCAAAACATGAG
GGTATCTTTTCAACTTTAAATAGAGACTATTCTTTTAGAGACTACTCAAGTGAATGCACA
CACAGTGAAAATTCTCGGAGTTGTGAGAACATGAATGGAACTTCTTACTATAACAGTCAT
AGCCACAGTGGGGAAGAAAACTTAAACCCTGTGCCTCAGCTGGACATTGGAACCTTGGAG
AATGTCTTCACAGCCCCGACATCAACTCCTTCTAGCATCCAGCAAGTCAATGTCACCGAC
AGTGATGAGGAGGAAGAAGAAAAAGTGCTCAGGGATTTA

ORF Protein

Cloned ORF protein sequence for pFN21AB6245 Download

>KIBB6245  753 aa
MERARPEPPPQPRPLRPAPPPLPVEGTSFWAAAMEPPPSSPTLSAAASATLASSCGEAVA
SGLQPAVRRLLQVKPEQVLLLPQPQAQNEEAAASSAQARLLQFRPDLRLLQPPTASDGAT
SRPELHPVQPLALHVKAKKQKLGPSLDQSVGPRGAVETGPRASRVVKLEGPGPALGYFRG
DEKGKLEAEEVMRDSMQGGAGKSPAAIREGVIKTEEPERLLEDCRLGAEPASNGLVHGSA
EVILAPTSGAFGPHQQDLRIPLTLHTVPPGARIQFQGAPPSELIRLTKVPLTPVPTKMQS
LLEPSVKIETKDVPLTVLPSDAGIPDTPFSKDRNGHVKRPMNAFMVWARIHRPALAKANP
AANNAEISVQLGLEWNKLSEEQKKPYYDEAQKIKEKHREEFPGWVYQPRPGKRKRFPLSV
SNVFSGTTQNIISTNPTTVYPYRSPTYSVVIPSLQNPITHPVGETSPAIQLPTPAVQSPS
PVTLFQPSVSSAAQVAVQDPSLPVYPALPPQRFTGPSQTDTHQLHSEATHTVKQPTPVSL
ESANRISSSASTAHARFATSTIQPPREYSSVSPCPRSAPIPQASPIPHPHVYQPPPLGHP
ATLFGTPPRFSFHHPYFLPGPHYFPSSTCPYSRPPFGYGNFPSSMPECLSYYEDRYPKHE
GIFSTLNRDYSFRDYSSECTHSENSRSCENMNGTSYYNSHSHSGEENLNPVPQLDIGTLE
NVFTAPTSTPSSIQQVNVTDSDEEEEEKVLRDL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6245 Download

>pFN21AB6245  6964 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGAGAGCCAGACCCGAGCCGCCGCCTCAGCC
GCGCCCGTTGCGTCCCGCTCCGCCCCCGCTGCCGGTCGAGGGCACCTCCTTTTGGGCAGC
AGCCATGGAGCCCCCTCCGTCGTCTCCCACACTGAGCGCGGCAGCCAGTGCGACCTTGGC
CTCGTCGTGCGGGGAGGCAGTGGCGTCCGGCTTACAGCCCGCGGTGCGGCGGCTGCTGCA
GGTGAAGCCAGAGCAGGTGTTGCTGCTACCACAGCCTCAGGCCCAGAACGAGGAAGCCGC
TGCCTCGTCCGCGCAGGCGCGGCTGTTGCAGTTCAGGCCCGACCTGCGGCTCCTGCAGCC
GCCGACAGCGTCAGACGGCGCCACCTCCAGGCCCGAGTTGCACCCGGTGCAGCCCCTGGC
GCTGCATGTCAAGGCCAAGAAGCAGAAGCTGGGGCCCAGCCTGGATCAGTCAGTGGGGCC
TCGAGGGGCCGTCGAAACCGGTCCTAGAGCCTCCAGGGTGGTCAAGTTGGAAGGCCCCGG
GCCGGCCCTCGGCTACTTCCGAGGGGACGAGAAGGGCAAGCTGGAGGCGGAGGAGGTCAT
GAGAGACTCGATGCAAGGCGGGGCAGGCAAAAGCCCGGCAGCCATCCGAGAAGGTGTGAT
CAAAACGGAGGAACCCGAGAGACTCCTCGAGGACTGCAGGCTCGGCGCGGAGCCCGCGTC
CAATGGCCTGGTTCATGGCAGCGCGGAGGTCATCTTGGCCCCAACGTCCGGTGCCTTTGG
GCCGCACCAGCAAGACCTTAGGATCCCTTTGACGCTCCACACGGTCCCCCCTGGGGCCCG
GATCCAGTTTCAGGGAGCTCCGCCTTCAGAGCTGATAAGATTGACCAAGGTCCCCCTGAC
ACCAGTGCCTACTAAAATGCAGTCCCTACTGGAGCCTTCTGTAAAAATTGAAACCAAAGA
TGTCCCGCTCACCGTGTTGCCCTCAGATGCAGGCATACCAGATACTCCCTTCAGTAAGGA
CAGAAATGGTCATGTGAAGCGACCCATGAACGCATTTATGGTTTGGGCAAGGATCCACCG
ACCAGCACTAGCCAAAGCTAACCCAGCAGCCAACAATGCAGAAATCAGTGTCCAGCTTGG
GTTAGAGTGGAACAAACTTAGTGAAGAACAAAAGAAACCCTATTACGATGAAGCACAAAA
GATTAAGGAAAAGCACAGAGAGGAATTTCCTGGTTGGGTTTATCAGCCTCGTCCAGGGAA
GCGAAAACGATTCCCTCTAAGTGTTTCCAATGTATTTTCTGGTACCACACAGAATATCAT
CTCTACAAATCCTACAACAGTTTATCCTTACCGCTCACCTACGTACTCTGTGGTAATTCC
CAGCCTACAGAATCCCATCACTCATCCAGTTGGTGAAACCTCACCTGCTATCCAGCTGCC
CACACCTGCAGTCCAGAGCCCAAGCCCTGTCACACTTTTCCAGCCCAGCGTCTCCAGTGC
TGCTCAGGTGGCTGTCCAGGATCCAAGTCTACCTGTCTATCCAGCACTCCCACCCCAACG
CTTTACTGGGCCTTCCCAAACAGACACTCATCAGCTGCATTCTGAAGCCACTCACACTGT
GAAGCAACCCACTCCTGTCTCTCTAGAGAGCGCCAACAGGATTTCAAGTAGTGCAAGTAC
TGCCCATGCCAGATTTGCAACTTCGACCATCCAACCTCCTAGGGAGTATTCCAGCGTTTC
CCCTTGTCCCAGAAGTGCTCCAATCCCCCAGGCTTCTCCCATTCCACACCCACATGTCTA
CCAGCCCCCTCCCCTTGGCCATCCAGCCACACTGTTCGGGACACCACCAAGATTCTCTTT
TCATCACCCTTACTTCCTACCCGGACCTCACTACTTCCCATCAAGTACATGCCCTTACAG
TCGGCCTCCCTTTGGCTATGGAAATTTTCCGAGTTCAATGCCAGAATGCCTTAGTTATTA
TGAAGACAGGTACCCAAAACATGAGGGTATCTTTTCAACTTTAAATAGAGACTATTCTTT
TAGAGACTACTCAAGTGAATGCACACACAGTGAAAATTCTCGGAGTTGTGAGAACATGAA
TGGAACTTCTTACTATAACAGTCATAGCCACAGTGGGGAAGAAAACTTAAACCCTGTGCC
TCAGCTGGACATTGGAACCTTGGAGAATGTCTTCACAGCCCCGACATCAACTCCTTCTAG
CATCCAGCAAGTCAATGTCACCGACAGTGATGAGGAGGAAGAAGAAAAAGTGCTCAGGGA
TTTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT