PIAS2 ORF clone pFN21AB6117

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6117

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Clone info.

Species

human

Clone Name

pFN21AB6117 Sequence (with vector) Restriction Map

Product ID

FHC03457

Flexi Clone PID

FXC03457

ORF Sequence

DNA sequence (1863 bp)
Protein sequence (621 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527541]

Gene

KIBB6117

Symbol

PIAS2

Alias : ARIP3, DIP, MIZ1, PIASX, SIZ2, ZMIZ4

Description

protein inhibitor of activated STAT, 2, transcript variant beta

Original Clone ID

cs00717

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1863 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 621 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32824.1 2.2e-191 100.0 PIAS2 CCDS32825.1 3.7e-170 100.0 PIAS2 CCDS45290.1 2.1e-82 57.1 PIAS1 CCDS81902.1 2.1e-82 56.7 PIAS1 CCDS72866.1 5e-81 59.8 PIAS3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004181 342 390 PF02891 Zinc finger HMMSmart IPR003034 11 45 SM00513 DNA-binding SAP ProfileScan IPR003034 11 45 PS50800 DNA-binding SAP IPR023321 134 299 PS51466 PINIT domain IPR004181 331 408 PS51044 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004662 621 603567 100.0 100.0 XP_005258436 622 603567 99.8 100.0 XP_006722635 660 603567 100.0 98.7 NP_001310977 612 603567 100.0 98.6 NP_001310978 612 603567 100.0 98.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6117 Download

>KIBB6117  1863 bp
ATGGCGGATTTCGAAGAGTTGAGGAATATGGTTTCTAGTTTTAGGGTTTCTGAACTACAA
GTATTACTAGGCTTTGCTGGACGGAATAAAAGTGGACGCAAGCATGACCTCCTGATGAGG
GCGCTGCATTTATTGAAGAGCGGCTGCAGCCCTGCGGTTCAGATTAAAATCCGAGAATTG
TATAGACGCCGATATCCACGAACTCTTGAAGGACTTTCTGATTTATCCACAATCAAATCA
TCGGTTTTCAGTTTGGATGGTGGCTCATCACCTGTAGAACCTGACTTGGCCGTGGCTGGA
ATCCACTCGTTGCCTTCCACTTCAGTTACACCTCACTCACCATCCTCTCCTGTTGGTTCT
GTGCTGCTTCAAGATACTAAGCCCACATTTGAGATGCAGCAGCCATCTCCCCCAATTCCT
CCTGTCCATCCTGATGTGCAGTTAAAAAATCTGCCCTTTTATGATGTCCTTGATGTTCTC
ATCAAGCCCACGAGTTTAGTTCAAAGCAGTATTCAGCGATTTCAAGAGAAGTTTTTTATT
TTTGCTTTGACACCTCAACAAGTTAGAGAGATATGCATATCCAGGGATTTTTTGCCAGGT
GGTAGGAGAGATTATACAGTCCAAGTTCAGTTGAGACTTTGCCTGGCAGAGACAAGTTGC
CCTCAAGAAGATAACTATCCAAATAGTCTATGTATAAAAGTAAATGGGAAGCTATTTCCT
TTGCCTGGCTATGCACCACCGCCTAAAAATGGGATTGAACAGAAGCGCCCTGGACGCCCC
TTGAATATTACATCTTTAGTTAGGTTATCTTCAGCTGTGCCAAACCAAATTTCCATTTCT
TGGGCATCAGAAATTGGGAAGAATTACTCTATGTCTGTATATCTTGTACGGCAGCTTACA
TCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTATTAGAAACCCTGATCATTCCAGA
GCACTAATTAAAGAAAAACTTACTGCAGATCCTGATAGTGAAATTGCTACAACTAGCCTT
CGGGTATCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCATGCCGTGCAGTG
ACTTGTACACATCTGCAGTGTTTTGATGCTGCCCTCTATCTACAAATGAATGAGAAAAAG
CCCACCTGGATTTGTCCTGTGTGTGACAAAAAAGCTGCCTATGAAAGTCTAATATTAGAT
GGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTAGATGAGATCAAATTCCAAGAA
GATGGTTCTTGGTGTCCAATGAGACCGAAGAAAGAAGCTATGAAAGTATCCAGCCAACCG
TGTACAAAAATAGAAAGTTCAAGCGTCCTCAGTAAGCCTTGTTCAGTGACTGTAGCCAGT
GAGGCAAGCAAGAAGAAAGTAGATGTTATTGATCTTACAATAGAAAGCTCTTCTGACGAA
GAGGAAGACCCTCCTGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGCCCA
ACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCGGTT
GATCCTGCTGCTATTCCGCCTTCATTAACAGACTACTCAGTACCATTCCACCATACGCCA
ATATCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGAT
CCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGT
ACGTCTGTCACCACCACCAGCTCCCATGAAAGCAGTACTCATGTTAGTTCATCCAGCAGC
AGGAGTGAGACAGGGGTCATAACCAGCAGTGGAAGTAACATTCCTGACATCATCTCATTG
GAC

ORF Protein

Cloned ORF protein sequence for pFN21AB6117 Download

>KIBB6117  621 aa
MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIREL
YRRRYPRTLEGLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGS
VLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFI
FALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFP
LPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLT
SAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAV
TCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQE
DGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDE
EEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTP
ISSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSS
RSETGVITSSGSNIPDIISLD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6117 Download

>pFN21AB6117  6568 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGATTTCGAAGAGTTGAGGAATATGGTTTC
TAGTTTTAGGGTTTCTGAACTACAAGTATTACTAGGCTTTGCTGGACGGAATAAAAGTGG
ACGCAAGCATGACCTCCTGATGAGGGCGCTGCATTTATTGAAGAGCGGCTGCAGCCCTGC
GGTTCAGATTAAAATCCGAGAATTGTATAGACGCCGATATCCACGAACTCTTGAAGGACT
TTCTGATTTATCCACAATCAAATCATCGGTTTTCAGTTTGGATGGTGGCTCATCACCTGT
AGAACCTGACTTGGCCGTGGCTGGAATCCACTCGTTGCCTTCCACTTCAGTTACACCTCA
CTCACCATCCTCTCCTGTTGGTTCTGTGCTGCTTCAAGATACTAAGCCCACATTTGAGAT
GCAGCAGCCATCTCCCCCAATTCCTCCTGTCCATCCTGATGTGCAGTTAAAAAATCTGCC
CTTTTATGATGTCCTTGATGTTCTCATCAAGCCCACGAGTTTAGTTCAAAGCAGTATTCA
GCGATTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAACAAGTTAGAGAGATATG
CATATCCAGGGATTTTTTGCCAGGTGGTAGGAGAGATTATACAGTCCAAGTTCAGTTGAG
ACTTTGCCTGGCAGAGACAAGTTGCCCTCAAGAAGATAACTATCCAAATAGTCTATGTAT
AAAAGTAAATGGGAAGCTATTTCCTTTGCCTGGCTATGCACCACCGCCTAAAAATGGGAT
TGAACAGAAGCGCCCTGGACGCCCCTTGAATATTACATCTTTAGTTAGGTTATCTTCAGC
TGTGCCAAACCAAATTTCCATTTCTTGGGCATCAGAAATTGGGAAGAATTACTCTATGTC
TGTATATCTTGTACGGCAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGG
TATTAGAAACCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGATCCTGA
TAGTGAAATTGCTACAACTAGCCTTCGGGTATCCTTGATGTGCCCTTTAGGAAAAATGAG
GCTGACAATCCCATGCCGTGCAGTGACTTGTACACATCTGCAGTGTTTTGATGCTGCCCT
CTATCTACAAATGAATGAGAAAAAGCCCACCTGGATTTGTCCTGTGTGTGACAAAAAAGC
TGCCTATGAAAGTCTAATATTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGA
TGTAGATGAGATCAAATTCCAAGAAGATGGTTCTTGGTGTCCAATGAGACCGAAGAAAGA
AGCTATGAAAGTATCCAGCCAACCGTGTACAAAAATAGAAAGTTCAAGCGTCCTCAGTAA
GCCTTGTTCAGTGACTGTAGCCAGTGAGGCAAGCAAGAAGAAAGTAGATGTTATTGATCT
TACAATAGAAAGCTCTTCTGACGAAGAGGAAGACCCTCCTGCCAAAAGGAAATGCATCTT
TATGTCAGAAACACAAAGCAGCCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGT
AAGGGTGCCCAGTGTGACTTCGGTTGATCCTGCTGCTATTCCGCCTTCATTAACAGACTA
CTCAGTACCATTCCACCATACGCCAATATCAAGCATGTCATCAGATTTGCCAGGTTTGGA
TTTTCTTTCCCTTATTCCAGTTGATCCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCT
CACCTCACCCTTAACAGCAAGCAGTACGTCTGTCACCACCACCAGCTCCCATGAAAGCAG
TACTCATGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCATAACCAGCAGTGGAAG
TAACATTCCTGACATCATCTCATTGGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT