PNKP ORF clone pFN21AB5985

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5985

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Clone info.

Species

human

Clone Name

pFN21AB5985 Sequence (with vector) Restriction Map

Product ID

FHC06413

Flexi Clone PID

FXC06413

ORF Sequence

DNA sequence (1563 bp)
Protein sequence (521 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464050]

Gene

KIBB5985

Symbol

PNKP

Alias : AOA4, CMT2B2, EIEE10, MCSZ, PNK

Description

polynucleotide kinase 3'-phosphatase

Original Clone ID

cs00685

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1563 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 521 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12783.1 6.4e-206 100.0 PNKP The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013954 166 328 PF08645 Polynucleotide kinase 3 phosphatase HMMTigr IPR006550 2 521 TIGR01663 Polynucleotide kinase 3-phosphatase IPR006551 153 328 TIGR01664 Polynucleotide 3'-phosphatase IPR006549 166 310 TIGR01662 HAD-superfamily hydrolase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009185 521 605610,613402,616267 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5985 Download

>KIBB5985  1563 bp
ATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCC
CCCATCTTCCTGCCCTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAG
GTTACGGACCGGAAGTGCTCCAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGG
ACAGTGGCAGTGAAACAGCTGGGAGTTAACCCCTCAACTACCGGGACCCAGGAGTTGAAG
CCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACACACTGTATTTGGTCAATGGCCTCCAC
CCACTGACCCTGCGCTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGATACTCCGCCT
GGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATG
CGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGG
GTGAAACCCCAGGGCAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGC
TCTGGGAAGGTCTTTCCCACTGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCC
CGTAAGCTCCGAGAGCTGGAAGCCGAGGGCTACAAGCTGGTGATCTTCACCAACCAGATG
AGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTTCAAGGCCAAGGTGGAGGCTGTGGTG
GAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTTGTACCGGAAG
CCGGTGACGGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCC
ATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGG
CGGAAGAAGAAAGACTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCC
TTCGCCACGCCTGAGGAGTTCTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCC
TTTGATCCGAGGACTGTCTCCCGCTCAGGGCCTCTCTGCCTCCCCGAGTCCAGGGCCCTC
CTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGGATTCCCTGGGGCCGGGAAGTCCACC
TTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGACACGCTAGGC
TCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCC
ATCGACAACACAAACCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCC
GCGGGCGTCCCCTGCCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAAC
AACCGGTTTCGAGAGATGACGGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTAT
GGCTACAGGAAGCAGTTCGAGGCCCCAACGCTGGCTGAAGGCTTCTCTGCCATCCTGGAG
ATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGGGCGGCTGTACTGCCAGTTCTCCGAG
GGC

ORF Protein

Cloned ORF protein sequence for pFN21AB5985 Download

>KIBB5985  521 aa
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETR
TVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPP
GTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTR
SGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVV
EKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPG
RKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRAL
LSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVA
IDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMY
GYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5985 Download

>pFN21AB5985  6268 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCT
CGAGAGCCCCCCTGGGGGAGCGCCCCCCATCTTCCTGCCCTCGGACGGGCAAGCCCTGGT
CCTGGGCAGGGGACCCCTGACCCAGGTTACGGACCGGAAGTGCTCCAGAACTCAAGTGGA
GCTGGTCGCAGATCCTGAGACCCGGACAGTGGCAGTGAAACAGCTGGGAGTTAACCCCTC
AACTACCGGGACCCAGGAGTTGAAGCCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACAC
ACTGTATTTGGTCAATGGCCTCCACCCACTGACCCTGCGCTGGGAAGAGACCCGCACACC
AGAATCCCAGCCAGATACTCCGCCTGGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGA
TGCTGAGCTGCCGAAGAAGCGTATGCGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAA
GTTGCTAGTGTTCACCGCAGCTGGGGTGAAACCCCAGGGCAAGGTGGCTGGCTTTGATCT
GGACGGGACGCTCATCACCACACGCTCTGGGAAGGTCTTTCCCACTGGCCCCAGTGACTG
GAGGATCTTGTACCCAGAGATTCCCCGTAAGCTCCGAGAGCTGGAAGCCGAGGGCTACAA
GCTGGTGATCTTCACCAACCAGATGAGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTT
CAAGGCCAAGGTGGAGGCTGTGGTGGAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGC
CACGCACGCAGGCTTGTACCGGAAGCCGGTGACGGGCATGTGGGACCATCTGCAGGAGCA
GGCCAACGACGGCACGCCCATATCCATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGG
ACGCCCGGCCAACTGGGCCCCGGGGCGGAAGAAGAAAGACTTCTCCTGCGCCGATCGCCT
GTTTGCCCTCAACCTTGGCCTGCCCTTCGCCACGCCTGAGGAGTTCTTTCTCAAGTGGCC
AGCAGCCGGCTTCGAGCTCCCAGCCTTTGATCCGAGGACTGTCTCCCGCTCAGGGCCTCT
CTGCCTCCCCGAGTCCAGGGCCCTCCTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGG
ATTCCCTGGGGCCGGGAAGTCCACCTTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGT
CCACGTGAACAGGGACACGCTAGGCTCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGC
CCTGAAGCAAGGGAAACGGGTCGCCATCGACAACACAAACCCAGACGCCGCGAGCCGCGC
CAGGTACGTCCAGTGTGCCCGAGCCGCGGGCGTCCCCTGCCGCTGCTTCCTCTTCACCGC
CACTCTGGAGCAGGCGCGCCACAACAACCGGTTTCGAGAGATGACGGACTCCTCTCATAT
CCCCGTGTCAGACATGGTCATGTATGGCTACAGGAAGCAGTTCGAGGCCCCAACGCTGGC
TGAAGGCTTCTCTGCCATCCTGGAGATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGG
GCGGCTGTACTGCCAGTTCTCCGAGGGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT