PPP2R2A ORF clone pFN21AB5514

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5514

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Clone info.

Species

human

Clone Name

pFN21AB5514 Sequence (with vector) Restriction Map

Product ID

FHC01820

Flexi Clone PID

FXC01820

ORF Sequence

DNA sequence (1341 bp)
Protein sequence (447 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385112]

Gene

KIBB5514

Symbol

PPP2R2A

Alias : B55A, B55ALPHA, PR52A, PR55A, PR55alpha

Description

protein phosphatase 2, regulatory subunit B, alpha, transcript variant 1

Original Clone ID

ef01695

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1341 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 447 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34867.1 1.7e-190 100.0 PPP2R2A CCDS55213.1 1.1e-189 100.0 PPP2R2A CCDS73224.1 1.7e-173 87.9 PPP2R2D CCDS64282.1 1.4e-169 87.7 PPP2R2B CCDS4283.1 8.9e-165 89.3 PPP2R2B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000009 35 55 PR00600 Protein phosphatase 2A IPR000009 70 98 PR00600 Protein phosphatase 2A IPR000009 99 127 PR00600 Protein phosphatase 2A IPR000009 176 203 PR00600 Protein phosphatase 2A IPR000009 204 231 PR00600 Protein phosphatase 2A IPR000009 232 260 PR00600 Protein phosphatase 2A IPR000009 261 288 PR00600 Protein phosphatase 2A IPR000009 289 316 PR00600 Protein phosphatase 2A IPR000009 317 342 PR00600 Protein phosphatase 2A IPR000009 343 369 PR00600 Protein phosphatase 2A IPR000009 413 442 PR00600 Protein phosphatase 2A HMMPfam IPR019781 95 123 PF00400 WD40 repeat IPR019781 228 253 PF00400 WD40 repeat HMMSmart IPR001680 21 56 SM00320 WD40 repeat IPR001680 83 123 SM00320 WD40 repeat IPR001680 165 204 SM00320 WD40 repeat IPR001680 215 255 SM00320 WD40 repeat IPR001680 274 312 SM00320 WD40 repeat IPR001680 339 370 SM00320 WD40 repeat IPR001680 406 443 SM00320 WD40 repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002708 447 604941 100.0 100.0 NP_001171062 457 604941 100.0 99.6 XP_005273616 446 604941 99.8 100.0 XP_011542867 456 604941 99.8 99.6 NP_060931 453 613992 87.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5514 Download

>KIBB5514  1341 bp
ATGGCAGGAGCTGGAGGAGGGAATGATATTCAGTGGTGTTTTTCTCAGGTGAAAGGAGCA
GTAGATGATGATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTTAATCATTCTGGA
GAATTACTAGCAACAGGAGATAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAGGAG
AACAAAATCCAGTCTCATAGCAGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCAT
GAACCAGAGTTTGACTACTTGAAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGG
TGGTTACCCCAGAAAAATGCTGCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAA
TTATGGAAAATCAGTGAAAGGGACAAAAGACCAGAAGGGTATAACTTGAAAGAGGAGGAT
GGAAGGTATAGAGATCCTACTACAGTTACTACACTACGAGTGCCAGTCTTTAGGCCTATG
GATCTAATGGTTGAGGCCAGTCCACGAAGAATATTTGCCAATGCTCATACATATCACATC
AACTCAATTTCTATTAATAGTGATTATGAAACATATTTATCTGCAGATGATTTGCGGATT
AATCTTTGGCATCTGGAAATTACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCC
AATATGGAAGAGCTAACAGAGGTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAAC
ACATTTGTATACAGCAGCAGTAAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCC
CTCTGTGATAGACATTCTAAATTGTTTGAAGAACCTGAAGATCCCAGTAACAGGTCATTT
TTTTCCGAAATCATCTCCTCTATTTCGGATGTAAAATTCAGCCATAGTGGTCGATATATG
ATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAATATGGAAAACAGGCCTGTG
GAAACATACCAGGTGCATGAATACCTCAGAAGTAAACTCTGTTCACTGTATGAAAATGAC
TGCATATTTGACAAATTTGAATGTTGTTGGAATGGATCTGACAGTGTTGTCATGACTGGA
TCTTACAATAATTTCTTCAGAATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAA
GCATCGCGGGAAAACAATAAGCCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGT
GGCAAGCGAAAGAAAGATGAAATAAGTGTTGACAGCCTAGACTTCAATAAGAAAATCCTT
CACACAGCCTGGCACCCCAAGGAAAATATCATTGCCGTAGCTACTACAAACAATCTGTAT
ATATTTCAAGACAAAGTGAAT

ORF Protein

Cloned ORF protein sequence for pFN21AB5514 Download

>KIBB5514  447 aa
MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQE
NKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIK
LWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHI
NSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCN
TFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYM
MTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTG
SYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKIL
HTAWHPKENIIAVATTNNLYIFQDKVN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5514 Download

>pFN21AB5514  6046 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGAGCTGGAGGAGGGAATGATATTCAGTG
GTGTTTTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAAGCAGATATAATTTC
TACAGTAGAATTTAATCATTCTGGAGAATTACTAGCAACAGGAGATAAAGGTGGTAGAGT
TGTCATCTTTCAACAGGAGCAGGAGAACAAAATCCAGTCTCATAGCAGAGGAGAATATAA
TGTTTACAGCACCTTCCAGAGCCATGAACCAGAGTTTGACTACTTGAAAAGTTTAGAAAT
AGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGCTGCTCAGTTTTTATT
GTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGGGACAAAAGACCAGA
AGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACTACAGTTACTACACT
ACGAGTGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGTCCACGAAGAATATT
TGCCAATGCTCATACATATCACATCAACTCAATTTCTATTAATAGTGATTATGAAACATA
TTTATCTGCAGATGATTTGCGGATTAATCTTTGGCATCTGGAAATTACAGACAGGAGTTT
TAACATTGTGGATATCAAGCCTGCCAATATGGAAGAGCTAACAGAGGTGATTACAGCAGC
AGAATTTCATCCAAACAGCTGTAACACATTTGTATACAGCAGCAGTAAAGGAACTATTCG
GCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCTAAATTGTTTGAAGAACC
TGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCTATTTCGGATGTAAA
ATTCAGCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGA
CTTAAATATGGAAAACAGGCCTGTGGAAACATACCAGGTGCATGAATACCTCAGAAGTAA
ACTCTGTTCACTGTATGAAAATGACTGCATATTTGACAAATTTGAATGTTGTTGGAATGG
ATCTGACAGTGTTGTCATGACTGGATCTTACAATAATTTCTTCAGAATGTTTGACAGAAA
CACAAAGCGAGACATAACCCTAGAAGCATCGCGGGAAAACAATAAGCCTCGCACAGTTCT
GAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAGAAAGATGAAATAAGTGTTGACAG
CCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAGGAAAATATCATTGC
CGTAGCTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAATGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT