KCNF1 ORF clone pFN21AB5407

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5407

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Clone info.

Species

human

Clone Name

pFN21AB5407 Sequence (with vector) Restriction Map

Product ID

FHC03558

Flexi Clone PID

FXC03558

ORF Sequence

DNA sequence (1482 bp)
Protein sequence (494 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527635]

Gene

KIBB5407

Symbol

KCNF1

Alias : IK8, KCNF, KV5.1, kH1

Description

potassium channel, voltage gated modifier subfamily F, member 1

Original Clone ID

bm04780

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1482 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 494 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1676.1 1.7e-206 100.0 KCNF1 CCDS13418.1 1.3e-73 41.9 KCNB1 CCDS6209.1 2.7e-72 40.6 KCNB2 CCDS6314.1 7.7e-53 37.5 KCNV1 CCDS12019.1 5e-50 32.5 KCNG2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003971 28 40 PR01494 Potassium channel IPR003971 68 81 PR01494 Potassium channel IPR003091 76 95 PR00169 Voltage-dependent potassium channel IPR003968 76 86 PR01491 Potassium channel IPR003971 92 107 PR01494 Potassium channel IPR003971 115 127 PR01494 Potassium channel IPR003091 175 203 PR00169 Voltage-dependent potassium channel IPR003091 224 247 PR00169 Voltage-dependent potassium channel IPR003091 250 270 PR00169 Voltage-dependent potassium channel IPR003091 294 320 PR00169 Voltage-dependent potassium channel IPR003968 299 307 PR01491 Potassium channel IPR003091 323 346 PR00169 Voltage-dependent potassium channel IPR003968 323 337 PR01491 Potassium channel IPR003091 354 376 PR00169 Voltage-dependent potassium channel IPR003091 383 409 PR00169 Voltage-dependent potassium channel IPR003971 393 411 PR01494 Potassium channel IPR003968 394 405 PR01491 Potassium channel HMMPfam IPR003131 25 126 PF02214 Potassium channel IPR005821 225 402 PF00520 Ion transport
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 181 ARVVAVLSFLLILVSSVVMCMGT 203 PRIMARY 23 2 251 ALSFMNIVDVLAILPFYVSLTLT 273 PRIMARY 23 3 320 SFKELGLLLMYLAVGIFVFSALG 342 SECONDARY 23 4 383 GKLNAAISFLCGVIAIALPIHPI 405 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002227 494 603787 100.0 100.0 XP_006723847 858 600397,616056 41.9 88.5 NP_004966 858 600397,616056 41.9 88.5 XP_011527101 858 600397,616056 41.9 88.5 NP_004761 911 607738 40.6 92.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5407 Download

>KIBB5407  1482 bp
ATGGACGGGTCCGGGGAGCGCAGCCTCCCGGAGCCGGGCAGCCAGAGCTCCGCTGCCAGC
GACGACATAGAGATAGTCGTCAACGTGGGGGGCGTGCGGCAGGTGCTGTACGGGGACCTC
CTCAGTCAGTACCCTGAGACCCGGCTGGCGGAGCTCATCAACTGCTTGGCTGGGGGCTAC
GACACCATCTTCTCCCTGTGCGACGACTACGACCCCGGCAAGCGCGAGTTCTACTTTGAC
AGGGACCCGGACGCCTTCAAGTGTGTCATCGAGGTGTACTATTTCGGGGAGGTCCACATG
AAGAAGGGCATCTGCCCCATCTGCTTCAAGAACGAGATGGACTTCTGGAAGGTGGACCTC
AAGTTCCTGGACGACTGTTGCAAGAGCCACCTGAGCGAGAAGCGCGAGGAGCTGGAGGAG
ATCGCGCGCCGCGTGCAGCTCATCCTGGACGACCTGGGCGTGGACGCGGCCGAGGGCCGC
TGGCGCCGCTGCCAGAAGTGCGTCTGGAAGTTCCTGGAGAAGCCCGAGTCGTCGTGCCCG
GCGCGGGTGGTGGCCGTGCTCTCCTTCCTGCTCATCCTCGTCTCGTCCGTGGTCATGTGC
ATGGGCACCATCCCCGAGCTGCAGGTGCTGGACGCCGAGGGCAACCGCGTGGAGCACCCG
ACGCTGGAGAACGTGGAGACGGCGTGCATTGGCTGGTTCACCCTGGAGTACCTGCTGCGC
CTCTTCTCGTCACCCAACAAGCTGCACTTCGCGCTGTCCTTCATGAACATTGTGGACGTG
CTGGCCATCCTCCCCTTCTACGTGAGCCTCACGCTCACGCACCTGGGTGCCCGCATGATG
GAGCTGACCAACGTGCAGCAGGCCGTGCAGGCGCTGCGGATCATGCGCATCGCGCGCATC
TTCAAGCTGGCCCGCCACTCCTCGGGCCTGCAGACCCTCACCTATGCCCTCAAGCGCAGC
TTCAAGGAACTGGGGCTGCTGCTCATGTACCTGGCAGTGGGTATCTTCGTCTTCTCTGCC
CTGGGCTACACCATGGAGCAGAGCCATCCAGAGACCCTGTTTAAGAGCATCCCCCAGTCC
TTCTGGTGGGCCATCATCACCATGACCACCGTCGGCTACGGCGACATCTACCCCAAGACC
ACGCTGGGCAAGCTCAACGCGGCCATCAGCTTCTTGTGTGGTGTCATCGCCATCGCCCTG
CCCATCCACCCCATCATCAACAACTTTGTCAGGTACTACAACAAGCAGCGCGTCCTGGAG
ACCGCGGCCAAGCACGAGCTGGAGCTGATGGAACTCAACTCCAGCAGCGGGGGCGAGGGC
AAGACCGGGGGCTCCCGCAGTGACCTGGACAACCTCCCTCCAGAGCCTGCGGGGAAGGAG
GCGCCGAGCTGCAGCAGCCGGCTGAAGCTCTCCCACAGCGACACCTTCATCCCCCTCCTG
ACCGAGGAGAAGCACCACAGGACCCGGCTCCAGAGTTGCAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB5407 Download

>KIBB5407  494 aa
MDGSGERSLPEPGSQSSAASDDIEIVVNVGGVRQVLYGDLLSQYPETRLAELINCLAGGY
DTIFSLCDDYDPGKREFYFDRDPDAFKCVIEVYYFGEVHMKKGICPICFKNEMDFWKVDL
KFLDDCCKSHLSEKREELEEIARRVQLILDDLGVDAAEGRWRRCQKCVWKFLEKPESSCP
ARVVAVLSFLLILVSSVVMCMGTIPELQVLDAEGNRVEHPTLENVETACIGWFTLEYLLR
LFSSPNKLHFALSFMNIVDVLAILPFYVSLTLTHLGARMMELTNVQQAVQALRIMRIARI
FKLARHSSGLQTLTYALKRSFKELGLLLMYLAVGIFVFSALGYTMEQSHPETLFKSIPQS
FWWAIITMTTVGYGDIYPKTTLGKLNAAISFLCGVIAIALPIHPIINNFVRYYNKQRVLE
TAAKHELELMELNSSSGGEGKTGGSRSDLDNLPPEPAGKEAPSCSSRLKLSHSDTFIPLL
TEEKHHRTRLQSCK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5407 Download

>pFN21AB5407  6187 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGGGTCCGGGGAGCGCAGCCTCCCGGAGCC
GGGCAGCCAGAGCTCCGCTGCCAGCGACGACATAGAGATAGTCGTCAACGTGGGGGGCGT
GCGGCAGGTGCTGTACGGGGACCTCCTCAGTCAGTACCCTGAGACCCGGCTGGCGGAGCT
CATCAACTGCTTGGCTGGGGGCTACGACACCATCTTCTCCCTGTGCGACGACTACGACCC
CGGCAAGCGCGAGTTCTACTTTGACAGGGACCCGGACGCCTTCAAGTGTGTCATCGAGGT
GTACTATTTCGGGGAGGTCCACATGAAGAAGGGCATCTGCCCCATCTGCTTCAAGAACGA
GATGGACTTCTGGAAGGTGGACCTCAAGTTCCTGGACGACTGTTGCAAGAGCCACCTGAG
CGAGAAGCGCGAGGAGCTGGAGGAGATCGCGCGCCGCGTGCAGCTCATCCTGGACGACCT
GGGCGTGGACGCGGCCGAGGGCCGCTGGCGCCGCTGCCAGAAGTGCGTCTGGAAGTTCCT
GGAGAAGCCCGAGTCGTCGTGCCCGGCGCGGGTGGTGGCCGTGCTCTCCTTCCTGCTCAT
CCTCGTCTCGTCCGTGGTCATGTGCATGGGCACCATCCCCGAGCTGCAGGTGCTGGACGC
CGAGGGCAACCGCGTGGAGCACCCGACGCTGGAGAACGTGGAGACGGCGTGCATTGGCTG
GTTCACCCTGGAGTACCTGCTGCGCCTCTTCTCGTCACCCAACAAGCTGCACTTCGCGCT
GTCCTTCATGAACATTGTGGACGTGCTGGCCATCCTCCCCTTCTACGTGAGCCTCACGCT
CACGCACCTGGGTGCCCGCATGATGGAGCTGACCAACGTGCAGCAGGCCGTGCAGGCGCT
GCGGATCATGCGCATCGCGCGCATCTTCAAGCTGGCCCGCCACTCCTCGGGCCTGCAGAC
CCTCACCTATGCCCTCAAGCGCAGCTTCAAGGAACTGGGGCTGCTGCTCATGTACCTGGC
AGTGGGTATCTTCGTCTTCTCTGCCCTGGGCTACACCATGGAGCAGAGCCATCCAGAGAC
CCTGTTTAAGAGCATCCCCCAGTCCTTCTGGTGGGCCATCATCACCATGACCACCGTCGG
CTACGGCGACATCTACCCCAAGACCACGCTGGGCAAGCTCAACGCGGCCATCAGCTTCTT
GTGTGGTGTCATCGCCATCGCCCTGCCCATCCACCCCATCATCAACAACTTTGTCAGGTA
CTACAACAAGCAGCGCGTCCTGGAGACCGCGGCCAAGCACGAGCTGGAGCTGATGGAACT
CAACTCCAGCAGCGGGGGCGAGGGCAAGACCGGGGGCTCCCGCAGTGACCTGGACAACCT
CCCTCCAGAGCCTGCGGGGAAGGAGGCGCCGAGCTGCAGCAGCCGGCTGAAGCTCTCCCA
CAGCGACACCTTCATCCCCCTCCTGACCGAGGAGAAGCACCACAGGACCCGGCTCCAGAG
TTGCAAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT