DDX19B ORF clone pFN21AB5389

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB5389

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Clone info.

Species

human

Clone Name

pFN21AB5389 Sequence (with vector) Restriction Map

Product ID

FHC10511

Flexi Clone PID

FXC10511

Original Clone PID

ORK10511

ORF Sequence

DNA sequence (1437 bp)
Protein sequence (479 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527308]

Gene

KIBB5389

Symbol

DDX19B

Alias : DBP5, DDX19, RNAh

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B, transcript variant 1

Original Clone ID

bm04585

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1437 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 479 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10888.1 3.7e-195 100.0 DDX19B CCDS10889.1 6.1e-189 96.0 DDX19A CCDS42187.1 2.5e-151 100.0 DDX19B CCDS32475.1 4.2e-142 93.5 DDX19B CCDS58478.1 4.2e-142 93.3 DDX19B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 117 281 PF00270 DNA/RNA helicase IPR001650 352 434 PF00271 Helicase HMMSmart IPR014001 111 310 SM00487 DEAD-like helicase IPR001650 347 434 SM00490 Helicase ProfileScan IPR014014 92 120 PS51195 RNA helicase IPR014001 125 295 PS51192 DEAD-like helicase IPR001650 306 474 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009173 479 605812 100.0 100.0 XP_011521134 539 605812 100.0 100.0 XP_011521136 484 605812 100.0 96.0 NP_001014451 448 605812 93.5 100.0 NP_001244101 453 605812 93.3 96.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5389 Download

>KIBB5389  1437 bp
ATGGCCACTGACTCATGGGCCCTGGCGGTGGACGAGCAGGAAGCTGCGGCTGAGTCGTTG
AGCAACTTGCATCTTAAGGAAGAGAAAATCAAACCAGATACCAATGGTGCTGTTGTCAAG
ACCAATGCCAATGCAGAGAAGACAGATGAAGAAGAGAAAGAGGACAGAGCTGCCCAGTCC
TTACTCAACAAGCTGATCAGAAGCAACCTTGTTGATAACACAAACCAAGTGGAAGTCCTG
CAGCGGGATCCAAACTCCCCTCTGTACTCGGTGAAGTCTTTTGAAGAGCTTCGGCTGAAA
CCACAGCTTCTCCAAGGAGTCTATGCCATGGGTTTCAATCGTCCATCCAAGATACAAGAG
AACGCATTGCCACTGATGCTTGCTGAGCCCCCACAGAACTTAATTGCCCAATCTCAGTCT
GGTACTGGTAAAACAGCTGCCTTCGTGCTGGCCATGCTTAGCCAAGTAGAACCTGCAAAC
AAATACCCCCAGTGTCTATGTCTCTCCCCAACGTATGAGCTCGCCCTCCAAACAGGAAAA
GTGATTGAACAAATGGGCAAATTTTACCCTGAACTGAAGCTAGCTTATGCTGTTCGAGGC
AATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCAGATTGTCATTGGCACCCCTGGGACT
GTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATCAAGGTGTTTGTT
CTGGATGAGGCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCATC
CAGAGGATGCTGCCCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCT
GTGTGGAAGTTTGCCCAGAAAGTGGTCCCAGACCCAAACGTTATCAAACTGAAGCGTGAG
GAAGAGACCCTGGACACCATCAAGCAGTACTATGTCCTGTGCAGCAGCAGAGACGAGAAG
TTCCAGGCCTTGTGTAACCTCTACGGGGCCATCACCATTGCTCAAGCCATGATCTTCTGC
CATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGAGCTCTCAAAAGAAGGCCACCAGGTG
GCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAGGGCTGCAGTGATTGAGCGCTTCCGA
GAGGGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTTGAA
CAAGTGTCTGTCGTCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAAT
GAGACCTACCTGCACCGGATCGGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTG
AACATGGTGGACAGCAAGCACAGCATGAACATCCTGAACAGAATCCAGGAGCATTTTAAT
AAGAAGATAGAAAGATTGGACACAGATGATTTGGACGAGATTGAGAAAATAGCCAAC

ORF Protein

Cloned ORF protein sequence for pFN21AB5389 Download

>KIBB5389  479 aa
MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQS
LLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQE
NALPLMLAEPPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGK
VIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFV
LDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKRE
EETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQV
ALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDN
ETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5389 Download

>pFN21AB5389  6142 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCACTGACTCATGGGCCCTGGCGGTGGACGA
GCAGGAAGCTGCGGCTGAGTCGTTGAGCAACTTGCATCTTAAGGAAGAGAAAATCAAACC
AGATACCAATGGTGCTGTTGTCAAGACCAATGCCAATGCAGAGAAGACAGATGAAGAAGA
GAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGCTGATCAGAAGCAACCTTGTTGA
TAACACAAACCAAGTGGAAGTCCTGCAGCGGGATCCAAACTCCCCTCTGTACTCGGTGAA
GTCTTTTGAAGAGCTTCGGCTGAAACCACAGCTTCTCCAAGGAGTCTATGCCATGGGTTT
CAATCGTCCATCCAAGATACAAGAGAACGCATTGCCACTGATGCTTGCTGAGCCCCCACA
GAACTTAATTGCCCAATCTCAGTCTGGTACTGGTAAAACAGCTGCCTTCGTGCTGGCCAT
GCTTAGCCAAGTAGAACCTGCAAACAAATACCCCCAGTGTCTATGTCTCTCCCCAACGTA
TGAGCTCGCCCTCCAAACAGGAAAAGTGATTGAACAAATGGGCAAATTTTACCCTGAACT
GAAGCTAGCTTATGCTGTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCA
GATTGTCATTGGCACCCCTGGGACTGTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGA
TCCCAAGAAAATCAAGGTGTTTGTTCTGGATGAGGCTGATGTCATGATAGCCACTCAGGG
CCACCAAGATCAGAGCATCCGCATCCAGAGGATGCTGCCCAGGAACTGCCAGATGCTGCT
TTTCTCCGCCACCTTTGAAGACTCTGTGTGGAAGTTTGCCCAGAAAGTGGTCCCAGACCC
AAACGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGGACACCATCAAGCAGTACTATGT
CCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTACGGGGCCATCAC
CATTGCTCAAGCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGA
GCTCTCAAAAGAAGGCCACCAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAG
GGCTGCAGTGATTGAGCGCTTCCGAGAGGGCAAAGAGAAGGTTTTGGTGACCACCAACGT
GTGTGCCCGCGGCATTGATGTTGAACAAGTGTCTGTCGTCATCAACTTTGATCTTCCCGT
GGACAAGGACGGGAATCCTGACAATGAGACCTACCTGCACCGGATCGGGCGCACGGGCCG
CTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACAGCAAGCACAGCATGAACATCCT
GAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACACAGATGATTTGGA
CGAGATTGAGAAAATAGCCAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT