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SpecieshumanProduct IDFHC01468Cloning SiteSgfI-PmeIGeneKIBB5093SymbolLGR4
Alias : BNMD17, GPR48Descriptionleucine-rich repeat containing G protein-coupled receptor 4Original Clone IDej00694
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 2853 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 951 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS31449.1 0 100.0 LGR4 CCDS9000.1 8.2e-75 50.9 LGR5 CCDS30971.1 1.8e-71 47.5 LGR6 CCDS1424.1 4.7e-66 47.8 LGR6 CCDS61195.1 3e-48 45.0 LGR5
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR002131 529 546 PR00373 Glycoprotein hormone receptor IPR000276 540 564 PR00237 GPCR IPR002131 605 618 PR00373 Glycoprotein hormone receptor IPR002131 618 632 PR00373 Glycoprotein hormone receptor IPR000276 625 647 PR00237 GPCR IPR000276 706 729 PR00237 GPCR IPR002131 741 759 PR00373 Glycoprotein hormone receptor IPR000276 783 809 PR00237 GPCR HMMPfam IPR000372 29 55 PF01462 Leucine-rich repeat-containing N-terminal IPR001611 61 80 PF00560 Leucine-rich repeat IPR001611 131 152 PF00560 Leucine-rich repeat IPR001611 154 176 PF00560 Leucine-rich repeat IPR001611 226 246 PF00560 Leucine-rich repeat IPR001611 345 364 PF00560 Leucine-rich repeat IPR001611 390 411 PF00560 Leucine-rich repeat IPR000276 556 801 PF00001 GPCR HMMSmart IPR000372 28 61 SM00013 Leucine-rich repeat-containing N-terminal IPR003591 55 79 SM00369 Leucine-rich repeat NULL 56 75 SM00364 NULL IPR003591 80 103 SM00369 Leucine-rich repeat NULL 104 130 SM00365 NULL IPR003591 104 127 SM00369 Leucine-rich repeat NULL 104 123 SM00364 NULL IPR003591 128 151 SM00369 Leucine-rich repeat NULL 128 147 SM00364 NULL NULL 152 178 SM00365 NULL IPR003591 152 175 SM00369 Leucine-rich repeat NULL 152 171 SM00364 NULL IPR003591 176 199 SM00369 Leucine-rich repeat NULL 176 195 SM00364 NULL NULL 200 226 SM00365 NULL IPR003591 200 223 SM00369 Leucine-rich repeat NULL 200 219 SM00364 NULL IPR003591 224 246 SM00369 Leucine-rich repeat NULL 224 243 SM00364 NULL IPR003591 247 270 SM00369 Leucine-rich repeat NULL 247 266 SM00364 NULL IPR003591 272 294 SM00369 Leucine-rich repeat IPR003591 318 341 SM00369 Leucine-rich repeat NULL 342 361 SM00364 NULL IPR003591 343 363 SM00369 Leucine-rich repeat IPR003591 364 387 SM00369 Leucine-rich repeat IPR003591 388 411 SM00369 Leucine-rich repeat NULL 412 435 SM00365 NULL IPR003591 412 435 SM00369 Leucine-rich repeat NULL 412 431 SM00364 NULL ProfileScan IPR001611 58 79 PS51450 Leucine-rich repeat IPR001611 82 103 PS51450 Leucine-rich repeat IPR001611 106 127 PS51450 Leucine-rich repeat IPR001611 130 151 PS51450 Leucine-rich repeat IPR001611 154 177 PS51450 Leucine-rich repeat IPR001611 178 199 PS51450 Leucine-rich repeat IPR001611 202 223 PS51450 Leucine-rich repeat IPR001611 226 247 PS51450 Leucine-rich repeat IPR001611 249 270 PS51450 Leucine-rich repeat IPR001611 273 294 PS51450 Leucine-rich repeat IPR001611 320 341 PS51450 Leucine-rich repeat IPR001611 344 365 PS51450 Leucine-rich repeat IPR001611 366 387 PS51450 Leucine-rich repeat IPR001611 390 411 PS51450 Leucine-rich repeat IPR001611 414 435 PS51450 Leucine-rich repeat IPR017452 555 801 PS50262 GPCR
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 2 PGPLGLLCFLALGLLGSAGPSGA 24 PRIMARY 23 2 541 IRLTVWFIFLVALFFNLLVILTT 563 PRIMARY 23 3 575 LFIGLISVSNLFMGIYTGILTFL 597 PRIMARY 23 4 619 KVAGFLAVFSSESAIFLLMLATV 641 SECONDARY 23 5 661 QFRVAALLAFLGATVAGCFPLFH 683 SECONDARY 23 6 705 GFTVTLVLLNSLAFLLMAVIYTK 727 PRIMARY 23 7 750 AWLIFTNCIFFCPVAFFSFAPLI 772 PRIMARY 23 8 784 SVTLIFFPLPACLNPVLYVFFNP 806 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060960 951 606666,615311 100.0 100.0 NP_001333361 927 606666,615311 97.5 100.0 NP_003658 907 606667 50.9 87.1 NP_001017403 967 606653 47.5 93.8 XP_011508141 900 606653 45.9 90.1 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AB5093 Download>KIBB5093 2853 bp ATGCCGGGCCCGCTAGGGCTGCTCTGCTTCCTCGCCCTGGGGCTGCTCGGCTCGGCCGGG CCCAGCGGCGCGGCGCCGCCTCTCTGCGCGGCGCCCTGCAGCTGCGACGGCGACCGTCGG GTGGACTGCTCCGGGAAGGGGCTGACGGCCGTGCCCGAGGGGCTCAGCGCCTTCACCCAA GCGCTGGATATCAGTATGAACAACATTACTCAGTTGCCAGAAGATGCATTTAAGAACTTT CCTTTTCTAGAAGAGCTACAATTGGCGGGCAACGACCTTTCTTTTATCCACCCAAAGGCC TTGTCTGGGTTGAAAGAACTCAAAGTTCTAACGCTCCAGAATAATCAGTTGAAAACAGTA CCCAGTGAAGCCATTCGAGGGCTGAGTGCTTTGCAGTCTTTGCGTTTAGATGCCAACCAT ATTACCTCAGTCCCCGAGGACAGTTTTGAAGGACTTGTTCAGTTACGGCATCTGTGGCTG GATGACAACAGCTTGACGGAGGTGCCTGTGCACCCCCTCAGCAATCTGCCCACCCTACAG GCGCTGACCCTGGCTCTCAACAAGATCTCAAGTATCCCTGACTTTGCATTTACCAACCTT TCAAGCCTGGTAGTTCTGCATCTTCATAACAATAAAATTAGAAGCCTGAGTCAACACTGT TTTGATGGACTAGATAACCTGGAGACCTTAGACTTGAATTATAATAACTTGGGGGAATTT CCTCAGGCTATTAAAGCCCTTCCTAGCCTTAAAGAGCTAGGATTTCATAGTAATTCTATT TCTGTTATCCCTGATGGAGCATTTGATGGTAATCCACTCTTAAGAACTATACATTTGTAT GATAATCCTCTGTCTTTTGTGGGGAACTCAGCATTTCACAATTTATCTGATCTTCATTCC CTAGTCATTCGTGGTGCAAGCATGGTGCAGCAGTTCCCCAATCTTACAGGAACTGTCCAC CTGGAAAGTCTGACTTTGACAGGTACAAAGATAAGCAGCATACCTAATAATTTGTGTCAA GAACAAAAGATGCTTAGGACTTTGGACTTGTCTTACAATAATATAAGAGACCTTCCAAGT TTTAATGGTTGCCATGCTCTGGAAGAAATTTCTTTACAGCGTAATCAAATCTACCAAATA AAGGAAGGCACCTTTCAAGGCCTGATATCTCTAAGGATTCTAGATCTGAGTAGAAACCTG ATACATGAAATTCACAGTAGAGCTTTTGCCACACTTGGGCCAATAACTAACCTAGATGTA AGTTTCAATGAATTAACTTCCTTTCCTACGGAAGGCCTGAATGGGCTAAATCAACTGAAA CTTGTGGGCAACTTCAAGCTGAAAGAAGCCTTAGCAGCAAAAGACTTTGTTAACCTCAGG TCTTTATCAGTACCATATGCTTATCAGTGCTGTGCATTTTGGGGTTGTGACTCTTATGCA AATTTAAACACAGAAGATAACAGCCTCCAGGACCACAGTGTGGCACAGGAGAAAGGTACT GCTGATGCAGCAAATGTCACAAGCACTCTTGAAAATGAAGAACATAGTCAAATAATTATC CATTGTACACCTTCAACAGGTGCTTTTAAGCCCTGTGAATATTTACTGGGAAGCTGGATG ATTCGTCTTACTGTGTGGTTCATTTTCTTGGTTGCATTATTTTTCAACCTGCTTGTTATT TTAACAACATTTGCATCTTGTACATCACTGCCTTCGTCCAAATTGTTTATAGGCTTGATT TCTGTGTCTAACTTATTCATGGGAATCTATACTGGCATCCTAACTTTTCTTGATGCTGTG TCCTGGGGCAGATTCGCTGAATTTGGCATTTGGTGGGAAACTGGCAGTGGCTGCAAAGTA GCTGGGTTTCTTGCAGTTTTCTCCTCAGAAAGTGCCATATTTTTATTAATGCTAGCAACT GTCGAAAGAAGCTTATCTGCAAAAGATATAATGAAAAATGGGAAGAGCAATCATCTCAAA CAGTTCCGGGTTGCTGCCCTTTTGGCTTTCCTAGGTGCTACAGTAGCAGGCTGTTTTCCC CTTTTCCATAGAGGGGAATATTCTGCATCACCCCTTTGTTTGCCATTTCCTACAGGTGAA ACGCCATCATTAGGATTCACTGTAACGTTAGTGCTATTAAACTCACTAGCATTTTTATTA ATGGCCGTTATCTACACTAAGCTATACTGCAACTTGGAAAAAGAGGACCTCTCAGAAAAC TCACAATCTAGCATGATTAAGCATGTCGCTTGGCTAATCTTCACCAATTGCATCTTTTTC TGCCCTGTGGCGTTTTTTTCATTTGCACCATTGATCACTGCAATCTCTATCAGCCCCGAA ATAATGAAGTCTGTTACTCTGATATTTTTTCCATTGCCTGCTTGCCTGAATCCAGTCCTG TATGTTTTCTTCAACCCAAAGTTTAAAGAAGACTGGAAGTTACTGAAGCGACGTGTTACC AAGAAAAGTGGATCAGTTTCAGTTTCCATCAGTAGCCAAGGTGGTTGTCTGGAACAGGAT TTCTACTACGACTGTGGCATGTACTCACATTTGCAGGGCAACCTGACTGTTTGCGACTGC TGCGAATCGTTTCTTTTAACAAAGCCAGTATCATGCAAACACTTGATAAAATCACACAGC TGTCCTGCATTGGCAGTGGCTTCTTGCCAAAGACCTGAGGGCTACTGGTCCGACTGTGGC ACACAGTCGGCCCACTCTGATTATGCAGATGAAGAAGATTCCTTTGTCTCAGACAGTTCT GACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTACCAGAGTAGAGGATTCCCTTTGGTG CGCTATGCTTACAATCTACCAAGAGTTAAAGAC
Cloned ORF protein sequence for pFN21AB5093 Download>KIBB5093 951 aa MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQ ALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTV PSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQ ALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEF PQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHS LVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPS FNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDV SFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYA NLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWM IRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAV SWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLK QFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLL MAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPE IMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQD FYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCG TQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD
Nucleotide Sequence (with vector) for pFN21AB5093 Download>pFN21AB5093 7558 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGCCGGGCCCGCTAGGGCTGCTCTGCTTCCTCGC CCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCGCGGCGCCGCCTCTCTGCGCGGCGCC CTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGGGCTGACGGCCGTGCC CGAGGGGCTCAGCGCCTTCACCCAAGCGCTGGATATCAGTATGAACAACATTACTCAGTT GCCAGAAGATGCATTTAAGAACTTTCCTTTTCTAGAAGAGCTACAATTGGCGGGCAACGA CCTTTCTTTTATCCACCCAAAGGCCTTGTCTGGGTTGAAAGAACTCAAAGTTCTAACGCT CCAGAATAATCAGTTGAAAACAGTACCCAGTGAAGCCATTCGAGGGCTGAGTGCTTTGCA GTCTTTGCGTTTAGATGCCAACCATATTACCTCAGTCCCCGAGGACAGTTTTGAAGGACT TGTTCAGTTACGGCATCTGTGGCTGGATGACAACAGCTTGACGGAGGTGCCTGTGCACCC CCTCAGCAATCTGCCCACCCTACAGGCGCTGACCCTGGCTCTCAACAAGATCTCAAGTAT CCCTGACTTTGCATTTACCAACCTTTCAAGCCTGGTAGTTCTGCATCTTCATAACAATAA AATTAGAAGCCTGAGTCAACACTGTTTTGATGGACTAGATAACCTGGAGACCTTAGACTT GAATTATAATAACTTGGGGGAATTTCCTCAGGCTATTAAAGCCCTTCCTAGCCTTAAAGA GCTAGGATTTCATAGTAATTCTATTTCTGTTATCCCTGATGGAGCATTTGATGGTAATCC ACTCTTAAGAACTATACATTTGTATGATAATCCTCTGTCTTTTGTGGGGAACTCAGCATT TCACAATTTATCTGATCTTCATTCCCTAGTCATTCGTGGTGCAAGCATGGTGCAGCAGTT CCCCAATCTTACAGGAACTGTCCACCTGGAAAGTCTGACTTTGACAGGTACAAAGATAAG CAGCATACCTAATAATTTGTGTCAAGAACAAAAGATGCTTAGGACTTTGGACTTGTCTTA CAATAATATAAGAGACCTTCCAAGTTTTAATGGTTGCCATGCTCTGGAAGAAATTTCTTT ACAGCGTAATCAAATCTACCAAATAAAGGAAGGCACCTTTCAAGGCCTGATATCTCTAAG GATTCTAGATCTGAGTAGAAACCTGATACATGAAATTCACAGTAGAGCTTTTGCCACACT TGGGCCAATAACTAACCTAGATGTAAGTTTCAATGAATTAACTTCCTTTCCTACGGAAGG CCTGAATGGGCTAAATCAACTGAAACTTGTGGGCAACTTCAAGCTGAAAGAAGCCTTAGC AGCAAAAGACTTTGTTAACCTCAGGTCTTTATCAGTACCATATGCTTATCAGTGCTGTGC ATTTTGGGGTTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCTCCAGGACCA CAGTGTGGCACAGGAGAAAGGTACTGCTGATGCAGCAAATGTCACAAGCACTCTTGAAAA TGAAGAACATAGTCAAATAATTATCCATTGTACACCTTCAACAGGTGCTTTTAAGCCCTG TGAATATTTACTGGGAAGCTGGATGATTCGTCTTACTGTGTGGTTCATTTTCTTGGTTGC ATTATTTTTCAACCTGCTTGTTATTTTAACAACATTTGCATCTTGTACATCACTGCCTTC GTCCAAATTGTTTATAGGCTTGATTTCTGTGTCTAACTTATTCATGGGAATCTATACTGG CATCCTAACTTTTCTTGATGCTGTGTCCTGGGGCAGATTCGCTGAATTTGGCATTTGGTG GGAAACTGGCAGTGGCTGCAAAGTAGCTGGGTTTCTTGCAGTTTTCTCCTCAGAAAGTGC CATATTTTTATTAATGCTAGCAACTGTCGAAAGAAGCTTATCTGCAAAAGATATAATGAA AAATGGGAAGAGCAATCATCTCAAACAGTTCCGGGTTGCTGCCCTTTTGGCTTTCCTAGG TGCTACAGTAGCAGGCTGTTTTCCCCTTTTCCATAGAGGGGAATATTCTGCATCACCCCT TTGTTTGCCATTTCCTACAGGTGAAACGCCATCATTAGGATTCACTGTAACGTTAGTGCT ATTAAACTCACTAGCATTTTTATTAATGGCCGTTATCTACACTAAGCTATACTGCAACTT GGAAAAAGAGGACCTCTCAGAAAACTCACAATCTAGCATGATTAAGCATGTCGCTTGGCT AATCTTCACCAATTGCATCTTTTTCTGCCCTGTGGCGTTTTTTTCATTTGCACCATTGAT CACTGCAATCTCTATCAGCCCCGAAATAATGAAGTCTGTTACTCTGATATTTTTTCCATT GCCTGCTTGCCTGAATCCAGTCCTGTATGTTTTCTTCAACCCAAAGTTTAAAGAAGACTG GAAGTTACTGAAGCGACGTGTTACCAAGAAAAGTGGATCAGTTTCAGTTTCCATCAGTAG CCAAGGTGGTTGTCTGGAACAGGATTTCTACTACGACTGTGGCATGTACTCACATTTGCA GGGCAACCTGACTGTTTGCGACTGCTGCGAATCGTTTCTTTTAACAAAGCCAGTATCATG CAAACACTTGATAAAATCACACAGCTGTCCTGCATTGGCAGTGGCTTCTTGCCAAAGACC TGAGGGCTACTGGTCCGACTGTGGCACACAGTCGGCCCACTCTGATTATGCAGATGAAGA AGATTCCTTTGTCTCAGACAGTTCTGACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTA CCAGAGTAGAGGATTCCCTTTGGTGCGCTATGCTTACAATCTACCAAGAGTTAAAGACGT TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT
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