NTN4 ORF clone pFN21AB4582

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Halotag ORF Clone pFN21AB4582

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Clone info.

Species

human

Clone Name

pFN21AB4582 Sequence (with vector) Restriction Map

Product ID

FHC01774

Flexi Clone PID

FXC01774

ORF Sequence

DNA sequence (1884 bp)
Protein sequence (628 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384963]

Gene

KIBB4582

Symbol

NTN4

Alias : PRO3091

Description

netrin 4

Original Clone ID

fk09435

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1884 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 628 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9054.1 7.8e-205 100.0 NTN4 CCDS5750.1 1.4e-52 40.5 LAMB1 CCDS83218.1 7e-52 41.8 LAMB4 CCDS34732.1 1.2e-51 41.8 LAMB4 CCDS2789.1 2.6e-51 39.4 LAMB2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 286 304 PR00011 NULL NULL 413 431 PR00011 NULL NULL 432 460 PR00011 NULL NULL 499 517 PR00011 NULL HMMPfam IPR008211 34 260 PF00055 Laminin IPR002049 262 318 PF00053 EGF-like IPR002049 332 383 PF00053 EGF-like IPR002049 395 443 PF00053 EGF-like IPR018933 518 621 PF01759 Netrin module HMMSmart IPR008211 28 260 SM00136 Laminin IPR002049 262 329 SM00180 EGF-like IPR002049 332 392 SM00180 EGF-like IPR002049 395 446 SM00180 EGF-like IPR018933 516 625 SM00643 Netrin module ProfileScan IPR008211 30 261 PS51117 Laminin IPR002049 262 331 PS50027 EGF-like IPR002049 332 394 PS50027 EGF-like IPR002049 395 448 PS50027 EGF-like IPR001134 506 627 PS50189 Netrin domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 ARLLLLWGCTVVAAGLSGVAGVS 27 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_067052 628 610401 100.0 100.0 NP_001316630 591 610401 100.0 94.1 NP_001316631 591 610401 100.0 94.1 NP_001316629 605 610401 96.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4582 Download

>KIBB4582  1884 bp
ATGGGGAGCTGCGCGCGGCTGCTGCTGCTCTGGGGCTGCACGGTGGTGGCCGCAGGACTG
AGTGGAGTAGCTGGAGTGAGTTCCCGCTGTGAAAAAGCCTGCAACCCTCGGATGGGAAAT
TTGGCTTTGGGGCGAAAACTCTGGGCAGACACCACCTGCGGTCAGAATGCTACCGAACTG
TACTGCTTCTACAGTGAGAACACGGATCTGACTTGTCGGCAGCCCAAATGTGACAAGTGC
AATGCTGCCTATCCTCACCTGGCTCACCTGCCATCTGCCATGGCAGACTCATCCTTCCGG
TTTCCTCGCACATGGTGGCAGTCTGCGGAGGATGTGCACAGAGAAAAGATCCAGTTAGAC
CTGGAAGCTGAATTCTACTTCACTCACCTAATTGTGATGTTCAAGTCCCCCAGGCCGGCT
GCCATGGTGCTGGACCGCTCCCAGGACTTTGGGAAAACATGGAAGCCTTATAAGTACTTT
GCGACTAACTGCTCCGCTACATTTGGCCTGGAAGATGATGTTGTCAAGAAGGGCGCTATT
TGTACTTCTAAATACTCCAGTCCTTTTCCATGCACTGGAGGAGAGGTTATTTTCAAAGCT
TTGTCACCACCATACGATACAGAGAACCCTTACAGTGCCAAAGTTCAGGAGCAGCTGAAG
ATCACCAACCTTCGCGTGCAGCTGCTGAAACGACAGTCTTGTCCCTGTCAGAGAAATGAC
CTGAACGAAGAGCCTCAACATTTTACACACTATGCAATCTATGATTTCATTGTCAAGGGC
AGCTGCTTCTGCAATGGCCACGCTGATCAATGCATACCTGTTCATGGCTTCAGACCTGTC
AAGGCCCCAGGAACATTCCACATGGTCCATGGGAAGTGTATGTGTAAGCACAACACAGCA
GGCAGCCACTGCCAGCACTGTGCCCCGTTATACAATGACCGGCCATGGGAGGCAGCTGAT
GGCAAAACGGGGGCTCCCAACGAGTGCAGAACCTGCAAGTGTAATGGGCATGCTGATACC
TGTCACTTCGACGTTAATGTGTGGGAGGCATCAGGGAATCGTAGTGGTGGTGTCTGTGAT
GACTGTCAGCACAACACAGAAGGACAGTATTGCCAGAGGTGCAAGCCAGGCTTCTATCGT
GACCTGCGGAGACCCTTCTCAGCTCCAGATGCTTGCAAACCGTGTTCCTGCCATCCAGTA
GGATCAGCTGTCCTTCCTGCCAACTCAGTGACCTTCTGCGACCCCAGCAATGGTGACTGC
CCTTGCAAGCCTGGGGTGGCAGGGCGACGTTGTGACAGGTGCATGGTGGGATACTGGGGC
TTCGGAGACTATGGCTGTCGACCATGTGACTGTGCGGGGAGCTGTGACCCTATCACCGGA
GACTGCATCAGCAGCCACACAGACATAGACTGGTATCATGAAGTTCCTGACTTCCGTCCC
GTGCACAATAAGAGCGAACCAGCCTGGGAGTGGGAGGATGCGCAGGGGTTTTCTGCACTT
CTACACTCAGGTAAATGCGAATGTAAGGAACAGACATTAGGAAATGCCAAGGCATTCTGT
GGAATGAAATATTCATATGTGCTAAAAATAAAGATTTTATCAGCTCATGATAAAGGTACT
CATGTTGAGGTCAATGTGAAGATTAAAAAGGTCTTAAAATCTACCAAACTGAAGATTTTC
CGAGGAAAGCGAACATTATATCCAGAATCATGGACGGACAGAGGATGCACTTGTCCAATC
CTCAATCCTGGTTTGGAATACCTTGTAGCAGGACATGAGGATATAAGAACAGGCAAACTA
ATTGTGAATATGAAAAGCTTTGTCCAGCACTGGAAACCTTCTCTTGGAAGAAAAGTCATG
GATATTTTAAAAAGAGAGTGCAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB4582 Download

>KIBB4582  628 aa
MGSCARLLLLWGCTVVAAGLSGVAGVSSRCEKACNPRMGNLALGRKLWADTTCGQNATEL
YCFYSENTDLTCRQPKCDKCNAAYPHLAHLPSAMADSSFRFPRTWWQSAEDVHREKIQLD
LEAEFYFTHLIVMFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAI
CTSKYSSPFPCTGGEVIFKALSPPYDTENPYSAKVQEQLKITNLRVQLLKRQSCPCQRND
LNEEPQHFTHYAIYDFIVKGSCFCNGHADQCIPVHGFRPVKAPGTFHMVHGKCMCKHNTA
GSHCQHCAPLYNDRPWEAADGKTGAPNECRTCKCNGHADTCHFDVNVWEASGNRSGGVCD
DCQHNTEGQYCQRCKPGFYRDLRRPFSAPDACKPCSCHPVGSAVLPANSVTFCDPSNGDC
PCKPGVAGRRCDRCMVGYWGFGDYGCRPCDCAGSCDPITGDCISSHTDIDWYHEVPDFRP
VHNKSEPAWEWEDAQGFSALLHSGKCECKEQTLGNAKAFCGMKYSYVLKIKILSAHDKGT
HVEVNVKIKKVLKSTKLKIFRGKRTLYPESWTDRGCTCPILNPGLEYLVAGHEDIRTGKL
IVNMKSFVQHWKPSLGRKVMDILKRECK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4582 Download

>pFN21AB4582  6589 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGAGCTGCGCGCGGCTGCTGCTGCTCTGGGG
CTGCACGGTGGTGGCCGCAGGACTGAGTGGAGTAGCTGGAGTGAGTTCCCGCTGTGAAAA
AGCCTGCAACCCTCGGATGGGAAATTTGGCTTTGGGGCGAAAACTCTGGGCAGACACCAC
CTGCGGTCAGAATGCTACCGAACTGTACTGCTTCTACAGTGAGAACACGGATCTGACTTG
TCGGCAGCCCAAATGTGACAAGTGCAATGCTGCCTATCCTCACCTGGCTCACCTGCCATC
TGCCATGGCAGACTCATCCTTCCGGTTTCCTCGCACATGGTGGCAGTCTGCGGAGGATGT
GCACAGAGAAAAGATCCAGTTAGACCTGGAAGCTGAATTCTACTTCACTCACCTAATTGT
GATGTTCAAGTCCCCCAGGCCGGCTGCCATGGTGCTGGACCGCTCCCAGGACTTTGGGAA
AACATGGAAGCCTTATAAGTACTTTGCGACTAACTGCTCCGCTACATTTGGCCTGGAAGA
TGATGTTGTCAAGAAGGGCGCTATTTGTACTTCTAAATACTCCAGTCCTTTTCCATGCAC
TGGAGGAGAGGTTATTTTCAAAGCTTTGTCACCACCATACGATACAGAGAACCCTTACAG
TGCCAAAGTTCAGGAGCAGCTGAAGATCACCAACCTTCGCGTGCAGCTGCTGAAACGACA
GTCTTGTCCCTGTCAGAGAAATGACCTGAACGAAGAGCCTCAACATTTTACACACTATGC
AATCTATGATTTCATTGTCAAGGGCAGCTGCTTCTGCAATGGCCACGCTGATCAATGCAT
ACCTGTTCATGGCTTCAGACCTGTCAAGGCCCCAGGAACATTCCACATGGTCCATGGGAA
GTGTATGTGTAAGCACAACACAGCAGGCAGCCACTGCCAGCACTGTGCCCCGTTATACAA
TGACCGGCCATGGGAGGCAGCTGATGGCAAAACGGGGGCTCCCAACGAGTGCAGAACCTG
CAAGTGTAATGGGCATGCTGATACCTGTCACTTCGACGTTAATGTGTGGGAGGCATCAGG
GAATCGTAGTGGTGGTGTCTGTGATGACTGTCAGCACAACACAGAAGGACAGTATTGCCA
GAGGTGCAAGCCAGGCTTCTATCGTGACCTGCGGAGACCCTTCTCAGCTCCAGATGCTTG
CAAACCGTGTTCCTGCCATCCAGTAGGATCAGCTGTCCTTCCTGCCAACTCAGTGACCTT
CTGCGACCCCAGCAATGGTGACTGCCCTTGCAAGCCTGGGGTGGCAGGGCGACGTTGTGA
CAGGTGCATGGTGGGATACTGGGGCTTCGGAGACTATGGCTGTCGACCATGTGACTGTGC
GGGGAGCTGTGACCCTATCACCGGAGACTGCATCAGCAGCCACACAGACATAGACTGGTA
TCATGAAGTTCCTGACTTCCGTCCCGTGCACAATAAGAGCGAACCAGCCTGGGAGTGGGA
GGATGCGCAGGGGTTTTCTGCACTTCTACACTCAGGTAAATGCGAATGTAAGGAACAGAC
ATTAGGAAATGCCAAGGCATTCTGTGGAATGAAATATTCATATGTGCTAAAAATAAAGAT
TTTATCAGCTCATGATAAAGGTACTCATGTTGAGGTCAATGTGAAGATTAAAAAGGTCTT
AAAATCTACCAAACTGAAGATTTTCCGAGGAAAGCGAACATTATATCCAGAATCATGGAC
GGACAGAGGATGCACTTGTCCAATCCTCAATCCTGGTTTGGAATACCTTGTAGCAGGACA
TGAGGATATAAGAACAGGCAAACTAATTGTGAATATGAAAAGCTTTGTCCAGCACTGGAA
ACCTTCTCTTGGAAGAAAAGTCATGGATATTTTAAAAAGAGAGTGCAAGGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT