NCKIPSD ORF clone pFN21AB4102

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB4102

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Clone info.

Species

human

Clone Name

pFN21AB4102 Sequence (with vector) Restriction Map

Product ID

FHC01760

Flexi Clone PID

FXC01760

ORF Sequence

DNA sequence (2145 bp)
Protein sequence (715 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384897]

Gene

KIBB4102

Symbol

NCKIPSD

Alias : AF3P21, DIP, DIP1, ORF1, SPIN90, VIP54, WASLBP, WISH

Description

NCK interacting protein with SH3 domain, transcript variant 2

Original Clone ID

fk00741

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2145 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 715 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46827.1 1.4e-147 100.0 NCKIPSD CCDS2776.1 8.5e-147 99.0 NCKIPSD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001452 3 49 PF00018 Src homology-3 domain IPR018556 540 678 PF09431 Domain of unknown function DUF2013 HMMSmart IPR001452 1 57 SM00326 Src homology-3 domain ProfileScan IPR001452 1 58 PS50002 Src homology-3 domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 454 LKCFGAMCSLDAAIISTLVSSVL 476 SECONDARY 23 2 493 KLCYSALILAMVFSMGEAVPYAH 515 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_909119 715 606671 100.0 100.0 NP_057537 722 606671 99.0 100.0 XP_016862084 755 606671 98.5 92.6 XP_016862083 762 606671 97.5 92.6 XP_016862089 637 606671 100.0 89.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4102 Download

>KIBB4102  2145 bp
ATGTACCGCGCGCTGTACGCGTTCCGCTCGGCGGAGCCCAACGCGCTGGCGTTCGCCGCG
GGCGAGACCTTCCTGGTGCTAGAGCGAAGCAGCGCGCACTGGTGGCTGGCCGCGCGGGCG
CGCAGTGGTGAGACGGGCTACGTGCCGCCAGCCTACCTGCGCCGCCTGCAGGGCCTGGAG
CAGGATGTCCTCCAGGCCATTGACCGGGCCATCGAGGCTGTACACAACACAGCCATGCGG
GATGGTGGCAAGTACAGCCTGGAACAGCGTGGAGTCCTCCAGAAGCTGATCCACCACCGG
AAAGAGACCCTGTCACGCAGAGGCCCTTCAGCCTCCAGTGTTGCAGTTATGACCTCATCA
ACCAGTGACCACCACTTGGATGCTGCTGCAGCCAGGCAGCCCAATGGGGTGTGTCGAGCT
GGGTTCGAGCGGCAGCACAGCCTACCCAGTTCTGAGCATCTTGGGGCAGATGGAGGCCTC
TACCAGATCCCACCACAGCCTCGCCGAGCAGCACCCACCACACCGCCCCCACCAGTGAAG
CGCCGAGACCGCGAGGCCCTGATGGCCTCTGGGAGTGGTGGCCACAACACCATGCCCTCC
GGGGGTAACTCTGTGTCCAGCGGCTCCTCAGTCAGCAGCACCTCCCTGGACACGCTCTAT
ACCAGCTCCAGCCCATCTGAACCAGGCTCCAGCTGCTCACCCACACCCCCACCTGTGCCC
CGCCGAGGCACCCACACCACCGTGTCCCAAGTCCAGCCCCCTCCCTCCAAGGCATCAGCA
CCTGAACCCCCTGCAGAAGAAGAAGTGGCAACTGGTACAACCTCAGCCTCTGATGACCTG
GAAGCCCTGGGTACACTGAGCCTGGGGACCACAGAGGAGAAGGCAGCAGCTGAGGCGGCT
GTGCCCAGGACCATTGGGGCCGAGCTGATGGAGCTGGTGCGGAGAAACACTGGCCTGAGC
CACGAATTATGCCGGGTGGCCATCGGCATCATAGTGGGTCACATCCAGGCCTCGGTGCCG
GCCAGCTCACCAGTCATGGAGCAGGTCCTCCTCTCACTCGTAGAGGGCAAGGACCTCAGC
ATGGCCCTGCCCTCAGGGCAGGTCTGCCACGACCAGCAGAGGCTGGAGGTGATCTTTGCA
GACCTGGCTCGCCGGAAGGACGACGCCCAGCAGCGCAGTTGGGCACTATATGAGGATGAG
GGTGTCATCCGCTGCTACCTAGAGGAGCTGCTGCATATTCTGACTGATGCAGACCCTGAA
GTTTGCAAGAAAATGTGCAAGAGAAACGAGTTCGAGTCTGTCCTGGCCTTGGTGGCCTAT
TACCAAATGGAACACCGAGCATCACTGCGGCTGCTGCTCCTCAAGTGCTTTGGCGCCATG
TGCAGCCTGGATGCAGCCATCATCTCCACGCTTGTGTCATCCGTGCTGCCTGTAGAGCTG
GCGAGGGACATGCAGACAGACACGCAGGACCACCAGAAACTCTGTTACTCTGCCCTCATC
CTGGCCATGGTCTTCTCCATGGGAGAGGCAGTGCCCTATGCACACTATGAGCACCTGGGC
ACGCCTTTCGCCCAGTTCCTACTGAACATCGTCGAGGATGGGCTGCCCTTGGACACCACA
GAGCAGCTGCCGGACCTCTGCGTGAACCTGCTTCTGGCTCTCAACCTGCACCTGCCAGCT
GCTGACCAGAATGTCATCATGGCTGCCCTGAGCAAACACGCCAATGTCAAGATCTTCTCC
GAGAAGCTGTTGTTGCTCCTGAACAGAGGGGATGACCCTGTGCGCATCTTCAAACATGAG
CCACAGCCACCACACTCTGTCCTCAAGTTCCTGCAGGACGTGTTTGGCAGCCCGGCCACA
GCTGCCATCTTCTACCACACAGACATGATGGCTCTCATTGACATCACTGTGCGGCACATC
GCAGACCTGTCACCAGGAGACAAGCTGCGCATGGAGTACCTCTCCCTGATGCATGCTATA
GTCCGCACCACACCCTACCTGCAGCACCGCCACCGGCTACCCGACCTGCAGGCCATACTG
CGACGCATCCTGAATGAGGAGGAGACCTCACCCCAGTGCCAGATGGACCGCATGATTGTC
CGAGAGATGTGCAAGGAATTCCTGGTGCTGGGGGAGGCTCCCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AB4102 Download

>KIBB4102  715 aa
MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLE
QDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSS
TSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPPQPRRAAPTTPPPPVK
RRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVP
RRGTHTTVSQVQPPPSKASAPEPPAEEEVATGTTSASDDLEALGTLSLGTTEEKAAAEAA
VPRTIGAELMELVRRNTGLSHELCRVAIGIIVGHIQASVPASSPVMEQVLLSLVEGKDLS
MALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEGVIRCYLEELLHILTDADPE
VCKKMCKRNEFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVEL
ARDMQTDTQDHQKLCYSALILAMVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTT
EQLPDLCVNLLLALNLHLPAADQNVIMAALSKHANVKIFSEKLLLLLNRGDDPVRIFKHE
PQPPHSVLKFLQDVFGSPATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAI
VRTTPYLQHRHRLPDLQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4102 Download

>pFN21AB4102  6850 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTACCGCGCGCTGTACGCGTTCCGCTCGGCGGA
GCCCAACGCGCTGGCGTTCGCCGCGGGCGAGACCTTCCTGGTGCTAGAGCGAAGCAGCGC
GCACTGGTGGCTGGCCGCGCGGGCGCGCAGTGGTGAGACGGGCTACGTGCCGCCAGCCTA
CCTGCGCCGCCTGCAGGGCCTGGAGCAGGATGTCCTCCAGGCCATTGACCGGGCCATCGA
GGCTGTACACAACACAGCCATGCGGGATGGTGGCAAGTACAGCCTGGAACAGCGTGGAGT
CCTCCAGAAGCTGATCCACCACCGGAAAGAGACCCTGTCACGCAGAGGCCCTTCAGCCTC
CAGTGTTGCAGTTATGACCTCATCAACCAGTGACCACCACTTGGATGCTGCTGCAGCCAG
GCAGCCCAATGGGGTGTGTCGAGCTGGGTTCGAGCGGCAGCACAGCCTACCCAGTTCTGA
GCATCTTGGGGCAGATGGAGGCCTCTACCAGATCCCACCACAGCCTCGCCGAGCAGCACC
CACCACACCGCCCCCACCAGTGAAGCGCCGAGACCGCGAGGCCCTGATGGCCTCTGGGAG
TGGTGGCCACAACACCATGCCCTCCGGGGGTAACTCTGTGTCCAGCGGCTCCTCAGTCAG
CAGCACCTCCCTGGACACGCTCTATACCAGCTCCAGCCCATCTGAACCAGGCTCCAGCTG
CTCACCCACACCCCCACCTGTGCCCCGCCGAGGCACCCACACCACCGTGTCCCAAGTCCA
GCCCCCTCCCTCCAAGGCATCAGCACCTGAACCCCCTGCAGAAGAAGAAGTGGCAACTGG
TACAACCTCAGCCTCTGATGACCTGGAAGCCCTGGGTACACTGAGCCTGGGGACCACAGA
GGAGAAGGCAGCAGCTGAGGCGGCTGTGCCCAGGACCATTGGGGCCGAGCTGATGGAGCT
GGTGCGGAGAAACACTGGCCTGAGCCACGAATTATGCCGGGTGGCCATCGGCATCATAGT
GGGTCACATCCAGGCCTCGGTGCCGGCCAGCTCACCAGTCATGGAGCAGGTCCTCCTCTC
ACTCGTAGAGGGCAAGGACCTCAGCATGGCCCTGCCCTCAGGGCAGGTCTGCCACGACCA
GCAGAGGCTGGAGGTGATCTTTGCAGACCTGGCTCGCCGGAAGGACGACGCCCAGCAGCG
CAGTTGGGCACTATATGAGGATGAGGGTGTCATCCGCTGCTACCTAGAGGAGCTGCTGCA
TATTCTGACTGATGCAGACCCTGAAGTTTGCAAGAAAATGTGCAAGAGAAACGAGTTCGA
GTCTGTCCTGGCCTTGGTGGCCTATTACCAAATGGAACACCGAGCATCACTGCGGCTGCT
GCTCCTCAAGTGCTTTGGCGCCATGTGCAGCCTGGATGCAGCCATCATCTCCACGCTTGT
GTCATCCGTGCTGCCTGTAGAGCTGGCGAGGGACATGCAGACAGACACGCAGGACCACCA
GAAACTCTGTTACTCTGCCCTCATCCTGGCCATGGTCTTCTCCATGGGAGAGGCAGTGCC
CTATGCACACTATGAGCACCTGGGCACGCCTTTCGCCCAGTTCCTACTGAACATCGTCGA
GGATGGGCTGCCCTTGGACACCACAGAGCAGCTGCCGGACCTCTGCGTGAACCTGCTTCT
GGCTCTCAACCTGCACCTGCCAGCTGCTGACCAGAATGTCATCATGGCTGCCCTGAGCAA
ACACGCCAATGTCAAGATCTTCTCCGAGAAGCTGTTGTTGCTCCTGAACAGAGGGGATGA
CCCTGTGCGCATCTTCAAACATGAGCCACAGCCACCACACTCTGTCCTCAAGTTCCTGCA
GGACGTGTTTGGCAGCCCGGCCACAGCTGCCATCTTCTACCACACAGACATGATGGCTCT
CATTGACATCACTGTGCGGCACATCGCAGACCTGTCACCAGGAGACAAGCTGCGCATGGA
GTACCTCTCCCTGATGCATGCTATAGTCCGCACCACACCCTACCTGCAGCACCGCCACCG
GCTACCCGACCTGCAGGCCATACTGCGACGCATCCTGAATGAGGAGGAGACCTCACCCCA
GTGCCAGATGGACCGCATGATTGTCCGAGAGATGTGCAAGGAATTCCTGGTGCTGGGGGA
GGCTCCCAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT