ST8SIA3 ORF clone pFN21AB3501

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB3501

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Clone info.

Species

human

Clone Name

pFN21AB3501 Sequence (with vector) Restriction Map

Product ID

FHC02099

Flexi Clone PID

FXC02099

ORF Sequence

DNA sequence (1140 bp)
Protein sequence (380 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463290]

Gene

KIBB3501

Symbol

ST8SIA3

Alias : SIAT8C, ST8SiaIII

Description

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3

Original Clone ID

ha06323

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1140 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 380 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32834.1 7.2e-172 100.0 ST8SIA3 CCDS81919.1 1.7e-50 39.1 ST8SIA2 CCDS10372.1 1.8e-50 39.1 ST8SIA2 CCDS4091.1 7.2e-47 39.3 ST8SIA4 CCDS31158.1 6.3e-39 35.8 ST8SIA6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001675 112 376 PF00777 Glycosyl transferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 9 VASVLGLVMLSVALLILSLISYV 31 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056963 380 609478 100.0 100.0 NP_001317345 354 602546 39.1 81.6 NP_006002 375 602546 39.1 81.6 XP_016878131 396 602546 39.1 81.6 NP_003025 356 601123 31.4 82.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3501 Download

>KIBB3501  1140 bp
ATGAGAAACTGCAAAATGGCCCGGGTCGCCAGTGTGCTGGGGCTGGTCATGCTCAGCGTC
GCCCTGCTGATTTTATCGCTCATCAGCTACGTGTCCCTGAAAAAGGAGAACATCTTCACC
ACTCCCAAGTACGCCAGCCCGGGGGCGCCCCGAATGTACATGTTCCACGCGGGATTCCGG
TCACAATTTGCGCTGAAGTTTCTAGACCCGTCATTCGTGCCCATTACGAATTCTCTCACC
CAGGAACTCCAAGAGAAACCTTCTAAGTGGAAATTTAATCGGACAGCGTTTTTACATCAA
AGGCAAGAAATTCTTCAGCATGTCGATGTAATAAAAAATTTTTCTTTGACCAAGAATAGT
GTTCGGATTGGACAACTGATGCACTATGATTATTCCAGCCATAAATATGTTTTCTCTATT
AGCAATAACTTCCGGTCACTTCTTCCAGATGTGTCACCCATTATGAACAAGCATTATAAT
ATTTGTGCTGTGGTTGGAAATAGTGGGATCCTGACAGGGAGCCAGTGTGGACAAGAAATA
GATAAATCAGATTTTGTTTTCCGTTGCAATTTCGCCCCTACGGAGGCTTTCCAAAGAGAT
GTTGGAAGAAAAACCAATCTTACCACCTTCAACCCCAGCATCCTGGAAAAATATTACAAC
AATCTCTTGACTATTCAGGACCGTAACAACTTTTTCCTCAGTTTAAAAAAGCTTGACGGG
GCCATTCTTTGGATCCCTGCATTTTTCTTCCACACTTCAGCAACTGTGACCAGGACATTA
GTTGACTTTTTTGTTGAACACAGAGGTCAGTTAAAAGTCCAACTGGCTTGGCCGGGAAAT
ATAATGCAACATGTCAACAGGTACTGGAAAAACAAACATTTGTCACCTAAACGGCTGAGC
ACAGGTATTCTTATGTACACCCTTGCATCAGCAATATGTGAAGAGATCCACTTGTATGGA
TTTTGGCCGTTTGGATTTGACCCCAACACAAGGGAAGATCTTCCATACCATTACTATGAC
AAAAAAGGAACCAAATTTACCACCAAGTGGCAGGAGTCCCACCAGCTGCCTGCTGAGTTT
CAGCTGCTGTACCGAATGCATGGGGAAGGGCTCACCAAGCTGACTCTGTCACACTGTGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB3501 Download

>KIBB3501  380 aa
MRNCKMARVASVLGLVMLSVALLILSLISYVSLKKENIFTTPKYASPGAPRMYMFHAGFR
SQFALKFLDPSFVPITNSLTQELQEKPSKWKFNRTAFLHQRQEILQHVDVIKNFSLTKNS
VRIGQLMHYDYSSHKYVFSISNNFRSLLPDVSPIMNKHYNICAVVGNSGILTGSQCGQEI
DKSDFVFRCNFAPTEAFQRDVGRKTNLTTFNPSILEKYYNNLLTIQDRNNFFLSLKKLDG
AILWIPAFFFHTSATVTRTLVDFFVEHRGQLKVQLAWPGNIMQHVNRYWKNKHLSPKRLS
TGILMYTLASAICEEIHLYGFWPFGFDPNTREDLPYHYYDKKGTKFTTKWQESHQLPAEF
QLLYRMHGEGLTKLTLSHCA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3501 Download

>pFN21AB3501  5845 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGAAACTGCAAAATGGCCCGGGTCGCCAGTGT
GCTGGGGCTGGTCATGCTCAGCGTCGCCCTGCTGATTTTATCGCTCATCAGCTACGTGTC
CCTGAAAAAGGAGAACATCTTCACCACTCCCAAGTACGCCAGCCCGGGGGCGCCCCGAAT
GTACATGTTCCACGCGGGATTCCGGTCACAATTTGCGCTGAAGTTTCTAGACCCGTCATT
CGTGCCCATTACGAATTCTCTCACCCAGGAACTCCAAGAGAAACCTTCTAAGTGGAAATT
TAATCGGACAGCGTTTTTACATCAAAGGCAAGAAATTCTTCAGCATGTCGATGTAATAAA
AAATTTTTCTTTGACCAAGAATAGTGTTCGGATTGGACAACTGATGCACTATGATTATTC
CAGCCATAAATATGTTTTCTCTATTAGCAATAACTTCCGGTCACTTCTTCCAGATGTGTC
ACCCATTATGAACAAGCATTATAATATTTGTGCTGTGGTTGGAAATAGTGGGATCCTGAC
AGGGAGCCAGTGTGGACAAGAAATAGATAAATCAGATTTTGTTTTCCGTTGCAATTTCGC
CCCTACGGAGGCTTTCCAAAGAGATGTTGGAAGAAAAACCAATCTTACCACCTTCAACCC
CAGCATCCTGGAAAAATATTACAACAATCTCTTGACTATTCAGGACCGTAACAACTTTTT
CCTCAGTTTAAAAAAGCTTGACGGGGCCATTCTTTGGATCCCTGCATTTTTCTTCCACAC
TTCAGCAACTGTGACCAGGACATTAGTTGACTTTTTTGTTGAACACAGAGGTCAGTTAAA
AGTCCAACTGGCTTGGCCGGGAAATATAATGCAACATGTCAACAGGTACTGGAAAAACAA
ACATTTGTCACCTAAACGGCTGAGCACAGGTATTCTTATGTACACCCTTGCATCAGCAAT
ATGTGAAGAGATCCACTTGTATGGATTTTGGCCGTTTGGATTTGACCCCAACACAAGGGA
AGATCTTCCATACCATTACTATGACAAAAAAGGAACCAAATTTACCACCAAGTGGCAGGA
GTCCCACCAGCTGCCTGCTGAGTTTCAGCTGCTGTACCGAATGCATGGGGAAGGGCTCAC
CAAGCTGACTCTGTCACACTGTGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT