EHD1 ORF clone pFN21AB3471

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Halotag ORF Clone pFN21AB3471

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Clone info.

Species

human

Clone Name

pFN21AB3471 Sequence (with vector) Restriction Map

Product ID

FHC01294

Flexi Clone PID

FXC01294

ORF Sequence

DNA sequence (1602 bp)
Protein sequence (534 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384808]

Gene

KIBB3471

Symbol

EHD1

Alias : H-PAST, HPAST1, PAST, PAST1

Description

EH-domain containing 1, transcript variant 2

Original Clone ID

ha03206

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1602 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 534 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8084.1 6.4e-207 100.0 EHD1 CCDS73315.1 6.5e-207 100.0 EHD1 CCDS1774.1 4.4e-185 87.0 EHD3 CCDS10081.1 2.4e-159 75.0 EHD4 CCDS12704.1 2.7e-150 71.5 EHD2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001401 61 221 PF00350 Dynamin HMMSmart IPR000261 438 531 SM00027 EPS15 homology (EH) ProfileScan IPR000261 444 532 PS50031 EPS15 homology (EH) IPR018249 476 511 PS50222 EF-HAND 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001269373 534 605888 100.0 100.0 XP_011543041 534 605888 100.0 100.0 NP_006786 534 605888 100.0 100.0 NP_001269374 548 605888 100.0 100.0 NP_055415 535 605891 87.0 99.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3471 Download

>KIBB3471  1602 bp
ATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACG
GTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAGAAGCTGCTACCCCTGGAGGAGCACTAC
CGCTTCCACGAGTTCCACTCGCCCGCGCTGGAGGACGCTGACTTCGACAACAAGCCTATG
GTGCTCCTCGTGGGGCAGTACAGCACGGGCAAGACCACCTTCATCCGACACCTGATCGAG
CAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCCCACCACCGACTCCTTCATCGCCGTC
ATGCACGGCCCCACTGAGGGCGTGGTGCCGGGCAACGCGCTCGTGGTGGACCCGCGGCGC
CCCTTCCGCAAGCTCAACGCGTTTGGCAACGCTTTCCTCAACAGGTTCATGTGTGCCCAG
CTGCCCAACCCCGTCCTGGACAGCATCAGCATCATCGACACCCCCGGGATCCTGTCTGGA
GAGAAGCAGCGGATCAGCAGAGGCTATGACTTTGCAGCCGTCCTGGAGTGGTTCGCGGAG
CGTGTGGACCGCATCATCCTGCTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTC
TCGGAAGTGATCAAGGCTCTGAAGAACCATGAGGACAAGATCCGCGTGGTGCTGAACAAG
GCAGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACGGGGCCCTCATGTGGTCCCTG
GGCAAGATCATCAACACCCCCGAGGTGGTCAGGGTCTACATCGGCTCCTTCTGGTCCCAC
CCGCTCCTCATCCCCGACAACCGCAAGCTCTTTGAGGCCGAGGAGCAGGACCTCTTCAAG
GACATCCAGTCACTGCCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAGCGG
GCACGGCTGGCCAAGGTTCACGCCTACATCATCAGCTCCCTCAAGAAAGAGATGCCCAAT
GTCTTTGGTAAAGAGAGCAAAAAGAAAGAGCTGGTGAACAACCTGGGAGAGATCTACCAG
AAGATTGAGCGCGAGCACCAGATCTCCCCTGGGGACTTCCCGAGCCTCCGCAAGATGCAG
GAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCGCTGAAGCCCAAGCTGCTGGAC
ACGGTGGATGACATGCTGGCCAACGACATCGCGCGGCTGATGGTGATGGTGCGGCAGGAG
GAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTTGACGGCACCATGAACGGG
CCGTTCGGGCACGGCTACGGCGAGGGGGCCGGCGAGGGCATCGACGACGTGGAGTGGGTG
GTGGGCAAGGACAAGCCCACCTACGACGAGATCTTCTACACGCTGTCCCCTGTCAACGGC
AAGATCACGGGCGCCAACGCCAAGAAGGAGATGGTGAAGTCCAAGCTCCCCAACACCGTG
CTAGGGAAGATCTGGAAGCTGGCCGACGTGGACAAGGACGGGCTGCTGGACGACGAGGAG
TTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCTGGAGGGCCACGAGCTGCCCGCCGAC
CTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAGACATGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB3471 Download

>KIBB3471  534 aa
MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPM
VLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRR
PFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAE
RVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSL
GKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKR
ARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQ
ELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNG
PFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTV
LGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3471 Download

>pFN21AB3471  6307 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGCAA
GAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAGAA
GCTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCACTCGCCCGCGCTGGAGGA
CGCTGACTTCGACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACGGGCAAGAC
CACCTTCATCCGACACCTGATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCC
CACCACCGACTCCTTCATCGCCGTCATGCACGGCCCCACTGAGGGCGTGGTGCCGGGCAA
CGCGCTCGTGGTGGACCCGCGGCGCCCCTTCCGCAAGCTCAACGCGTTTGGCAACGCTTT
CCTCAACAGGTTCATGTGTGCCCAGCTGCCCAACCCCGTCCTGGACAGCATCAGCATCAT
CGACACCCCCGGGATCCTGTCTGGAGAGAAGCAGCGGATCAGCAGAGGCTATGACTTTGC
AGCCGTCCTGGAGTGGTTCGCGGAGCGTGTGGACCGCATCATCCTGCTCTTCGACGCCCA
CAAGCTGGACATCTCCGATGAGTTCTCGGAAGTGATCAAGGCTCTGAAGAACCATGAGGA
CAAGATCCGCGTGGTGCTGAACAAGGCAGACCAGATCGAGACGCAGCAGCTGATGCGGGT
GTACGGGGCCCTCATGTGGTCCCTGGGCAAGATCATCAACACCCCCGAGGTGGTCAGGGT
CTACATCGGCTCCTTCTGGTCCCACCCGCTCCTCATCCCCGACAACCGCAAGCTCTTTGA
GGCCGAGGAGCAGGACCTCTTCAAGGACATCCAGTCACTGCCCCGAAACGCCGCCCTCAG
GAAGCTCAATGACCTGATCAAGCGGGCACGGCTGGCCAAGGTTCACGCCTACATCATCAG
CTCCCTCAAGAAAGAGATGCCCAATGTCTTTGGTAAAGAGAGCAAAAAGAAAGAGCTGGT
GAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAGCACCAGATCTCCCCTGGGGA
CTTCCCGAGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCA
GGCGCTGAAGCCCAAGCTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCGCG
GCTGATGGTGATGGTGCGGCAGGAGGAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGG
CGCCTTTGACGGCACCATGAACGGGCCGTTCGGGCACGGCTACGGCGAGGGGGCCGGCGA
GGGCATCGACGACGTGGAGTGGGTGGTGGGCAAGGACAAGCCCACCTACGACGAGATCTT
CTACACGCTGTCCCCTGTCAACGGCAAGATCACGGGCGCCAACGCCAAGAAGGAGATGGT
GAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAGATCTGGAAGCTGGCCGACGTGGACAA
GGACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCT
GGAGGGCCACGAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAG
ACATGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT