BTRC ORF clone pFN21AB3324

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Halotag ORF Clone pFN21AB3324

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Clone info.

Species

human

Clone Name

pFN21AB3324 Sequence (with vector) Restriction Map

Product ID

FHC11937

Flexi Clone PID

FXC11937

ORF Sequence

DNA sequence (1815 bp)
Protein sequence (605 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587367]

Gene

KIBB3324

Symbol

BTRC

Alias : BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP

Description

beta-transducin repeat containing E3 ubiquitin protein ligase, transcript variant 1

Original Clone ID

cs03071

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1815 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 605 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7512.1 4.1e-197 100.0 BTRC CCDS7511.1 6.7e-180 94.0 BTRC CCDS73183.1 5.5e-171 95.7 BTRC CCDS34289.1 1.2e-143 76.2 FBXW11 CCDS47340.1 1.5e-142 86.7 FBXW11 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 356 370 PR00320 G-protein beta WD-40 repeat IPR020472 479 493 PR00320 G-protein beta WD-40 repeat IPR020472 519 533 PR00320 G-protein beta WD-40 repeat HMMPfam IPR021977 138 177 PF12125 D domain of beta-TrCP IPR001810 192 229 PF00646 F-box domain IPR019781 304 329 PF00400 WD40 repeat IPR019781 334 369 PF00400 WD40 repeat IPR019781 374 409 PF00400 WD40 repeat IPR019781 420 452 PF00400 WD40 repeat IPR019781 457 492 PF00400 WD40 repeat IPR019781 497 532 PF00400 WD40 repeat IPR019781 547 581 PF00400 WD40 repeat HMMSmart IPR001810 189 228 SM00256 F-box domain IPR001680 292 329 SM00320 WD40 repeat IPR001680 332 369 SM00320 WD40 repeat IPR001680 372 409 SM00320 WD40 repeat IPR001680 415 452 SM00320 WD40 repeat IPR001680 455 492 SM00320 WD40 repeat IPR001680 495 532 SM00320 WD40 repeat IPR001680 544 581 SM00320 WD40 repeat ProfileScan IPR001810 190 228 PS50181 F-box domain IPR017986 299 590 PS50294 WD40-repeat-containing domain IPR019782 312 329 PS50082 WD40 repeat 2 IPR019782 339 378 PS50082 WD40 repeat 2 IPR019782 379 418 PS50082 WD40 repeat 2 IPR019782 422 461 PS50082 WD40 repeat 2 IPR019782 462 501 PS50082 WD40 repeat 2 IPR019782 502 533 PS50082 WD40 repeat 2 IPR019782 551 590 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_378663 605 603482 100.0 100.0 XP_006718117 732 603482 100.0 97.4 XP_016872361 587 603482 100.0 97.0 XP_016872362 587 603482 100.0 97.0 XP_016872360 587 603482 100.0 97.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3324 Download

>KIBB3324  1815 bp
ATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCC
AGGTCTCTGTGGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTG
TATAACCCAGGGACTGGCGCACTCACAGCTTTCCAGAATTCCTCAGAGAGAGAAGACTGT
AATAATGGCGAACCCCCTAGGAAGATAATACCAGAGAAGAATTCACTTAGACAGACATAC
AACAGCTGTGCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTAGCAAGCACTGCTATG
AAGACTGAGAATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAGTATGATTGTG
CCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATAC
TTTGAGCAGTGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATG
TGTCATTACCAACATGGGCACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTC
ATAACTGCTCTGCCAGCTCGGGGATTGGATCATATTGCTGAGAACATTCTGTCATACCTG
GATGCCAAATCACTATGTGCTGCTGAACTTGTGTGCAAGGAATGGTACCGAGTGACCTCT
GATGGCATGCTGTGGAAGAAGCTTATCGAGAGAATGGTCAGGACAGATTCTCTGTGGAGA
GGCCTGGCAGAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAACCTCCTGACGGG
AATGCTCCTCCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATTGAG
ACAATAGAATCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGT
GAAACAAGCAAAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTT
CGAGACAACACAATCAAGATCTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCACA
GGCCATACAGGTTCAGTCCTCTGTCTCCAGTATGATGAGAGAGTGATCATAACAGGATCA
TCGGATTCCACGGTCAGAGTGTGGGATGTAAATACAGGTGAAATGCTAAACACGTTGATT
CACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAATAATGGCATGATGGTGACCTGCTCC
AAAGATCGTTCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACCCTCCGGAGG
GTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTT
TCTGCATCTGGGGATAGAACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGG
ACCTTAAATGGACACAAACGAGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAGTG
AGTGGCTCATCTGACAACACTATCAGATTATGGGACATAGAATGTGGTGCATGTTTACGA
GTGTTAGAAGGCCATGAGGAATTGGTGCGTTGTATTCGATTTGATAACAAGAGGATAGTC
AGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGATCTTGTGGCTGCTTTGGACCCCCGT
GCTCCTGCAGGGACACTCTGTCTACGGACCCTTGTGGAGCATTCCGGAAGAGTTTTTCGA
CTACAGTTTGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTCATCTGG
GACTTCCTAAATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATAC
ACCTACATCTCCAGA

ORF Protein

Cloned ORF protein sequence for pFN21AB3324 Download

>KIBB3324  605 aa
MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDC
NNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIV
PKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDF
ITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWR
GLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRS
ETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGS
SDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRR
VLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVV
SGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPR
APAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTY
TYISR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3324 Download

>pFN21AB3324  6520 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGC
ACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTGTGGCTGGGCTGCTCCAGCCTGGCGGA
CAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGCGCACTCACAGCTTTCCA
GAATTCCTCAGAGAGAGAAGACTGTAATAATGGCGAACCCCCTAGGAAGATAATACCAGA
GAAGAATTCACTTAGACAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGAAAC
AGTATGTTTAGCAAGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACTTGC
CAATGGCACTTCCAGTATGATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAA
GGAAAAGGAACTGTGTGTCAAATACTTTGAGCAGTGGTCAGAGTCAGATCAAGTGGAATT
TGTGGAACATCTTATATCCCAAATGTGTCATTACCAACATGGGCACATAAACTCGTATCT
TAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCGGGGATTGGATCATAT
TGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTTGTGTG
CAAGGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAGAAT
GGTCAGGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAGAGGATGGGGACAGTATTT
ATTCAAAAACAAACCTCCTGACGGGAATGCTCCTCCCAACTCTTTTTATAGAGCACTTTA
TCCTAAAATTATACAAGACATTGAGACAATAGAATCTAATTGGAGATGTGGAAGACATAG
TTTACAGAGAATTCACTGCCGAAGTGAAACAAGCAAAGGAGTTTACTGTTTACAGTATGA
TGATCAGAAAATAGTAAGCGGCCTTCGAGACAACACAATCAAGATCTGGGATAAAAACAC
ATTGGAATGCAAGCGAATTCTCACAGGCCATACAGGTTCAGTCCTCTGTCTCCAGTATGA
TGAGAGAGTGATCATAACAGGATCATCGGATTCCACGGTCAGAGTGTGGGATGTAAATAC
AGGTGAAATGCTAAACACGTTGATTCACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAA
TAATGGCATGATGGTGACCTGCTCCAAAGATCGTTCCATTGCTGTATGGGATATGGCCTC
CCCAACTGACATTACCCTCCGGAGGGTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGT
AGACTTTGATGACAAGTACATTGTTTCTGCATCTGGGGATAGAACTATAAAGGTATGGAA
CACAAGTACTTGTGAATTTGTAAGGACCTTAAATGGACACAAACGAGGCATTGCCTGTTT
GCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCATCTGACAACACTATCAGATTATGGGA
CATAGAATGTGGTGCATGTTTACGAGTGTTAGAAGGCCATGAGGAATTGGTGCGTTGTAT
TCGATTTGATAACAAGAGGATAGTCAGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGA
TCTTGTGGCTGCTTTGGACCCCCGTGCTCCTGCAGGGACACTCTGTCTACGGACCCTTGT
GGAGCATTCCGGAAGAGTTTTTCGACTACAGTTTGATGAATTCCAGATTGTCAGTAGTTC
ACATGATGACACAATCCTCATCTGGGACTTCCTAAATGATCCAGCTGCCCAAGCTGAACC
CCCCCGTTCCCCTTCTCGAACATACACCTACATCTCCAGAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT