MID1 ORF clone pFN21AB3230

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Halotag ORF Clone pFN21AB3230

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Clone info.

Species

human

Clone Name

pFN21AB3230 Sequence (with vector) Restriction Map

Product ID

FHC01257

Flexi Clone PID

FXC01257

Original Clone PID

ORK01257

ORF Sequence

DNA sequence (1887 bp)
Protein sequence (629 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384764]

Gene

KIBB3230

Symbol

MID1

Alias : BBBG1, FXY, GBBB1, MIDIN, OGS1, OS, OSX, RNF59, TRIM18, XPRF, ZNFXY

Description

midline 1, transcript variant 5

Original Clone ID

fk07692

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1887 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 629 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14138.1 6.2e-130 94.3 MID1 CCDS14533.2 1.6e-99 71.2 MID2 CCDS75953.1 8.6e-62 98.6 MID1 CCDS75952.1 1e-52 81.5 MID1 CCDS14532.2 4.7e-50 66.8 MID2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003879 444 461 PR01407 Butyrophylin-like IPR003879 485 509 PR01407 Butyrophylin-like IPR003879 557 581 PR01407 Butyrophylin-like IPR003879 587 605 PR01407 Butyrophylin-like HMMPfam IPR018957 10 59 PF00097 Zinc finger IPR003961 357 433 PF00041 Fibronectin IPR003877 500 611 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR001841 10 59 SM00184 Zinc finger IPR000315 114 164 SM00336 Zinc finger IPR003649 181 307 SM00502 B-box IPR003961 344 434 SM00060 Fibronectin IPR018355 500 619 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001841 10 60 PS50089 Zinc finger IPR017903 282 341 PS51262 COS domain IPR003961 340 443 PS50853 Fibronectin IPR001870 428 621 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016885026 629 189960,300000,300552 100.0 100.0 XP_016885028 629 189960,300000,300552 100.0 100.0 XP_016885027 629 189960,300000,300552 100.0 100.0 NP_150631 629 189960,300000,300552 100.0 100.0 XP_016885025 667 189960,300000,300552 94.3 99.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3230 Download

>KIBB3230  1887 bp
ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCT
CTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCA
CACTGTGCCACCAACGAGTCTGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGG
CATGTCATCACCCTCAGCCAGCGAGGTCTAGACGGGCTCAAGCGCAACGTCACCCTACAG
AACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGCCCAACTCTCCCAGCGAGACC
CGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGTCCTCTGCCAG
TTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC
TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGT
CTGATTGAGCCAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGAT
GAGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGAACACAGAACTGGAG
ACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACGTCAA
GAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAG
ATTATTGGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAG
ATTGCAAACTGCAAACAGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACAC
TCTCTGAAGGAGAATGATCATGCGCGTTTCCTACAGACTGCTAAGAATATCACCGAGAGA
GTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATTCCTGAAATCAACCTCAATGACACA
TTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAGAATGTCTGGATTAC
CTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGACACC
ATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTAC
ACCATATTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATG
ATAGTACCCAACATCAAGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAG
TACATCTTCATGGTCAAGGCCATCAACCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAG
TTGAAGACAAACAGCCAACCATTTAAACTGGATCCCAAATCTGCTCATCGAAAACTGAAG
GTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCAAGAAGAGTCACACA
CCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGATAGT
GGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCT
TACAAATCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGC
CGCTGCAACAATAACTGGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCC
CCCCACCTCCGGCGCGTGGGCATCCTGCTGGACTATGATAACGGCTCTATCGCCTTTTAT
GATGCTTTGAACTCCATCCACCTCTACACCTTCGACGTCGCATTTGCGCAGCCTGTTTGC
CCCACCTTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGCTCCCTATCCCAGAC
CATTTGGACTGCACAGAGCAGCTGCCG

ORF Protein

Cloned ORF protein sequence for pFN21AB3230 Download

>KIBB3230  629 aa
METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCR
HVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQ
FCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHED
EKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQ
IIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITER
VSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDT
ITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTK
YIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHT
PERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALC
RCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVC
PTFTVWNKCLTIITGLPIPDHLDCTEQLP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3230 Download

>pFN21AB3230  6592 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAACACTGGAGTCAGAACTGACCTGCCCTAT
TTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGCCCTGCGCACACAGCCTCTGCTTCAA
CTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTCTGTGGAGTCCATCAC
CGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTAGACGG
GCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAG
CGGGCCCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGAC
CTCCGCCGAGAAGGTCCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAA
GACCTGTGTCACTTGTGAAGTATCCTACTGTGACGAGTGCCTGAAAGCCACTCACCCGAA
TAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGCCAATTCCGGACTCTCACATCCGGGG
GCTGATGTGCTTGGAGCATGAGGATGAGAAGCAAAACTTAGAGAGTAACCTCACCAACCT
TATTAAGAGGAACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACA
TGTTGAAGTCAATGCATCACGTCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCAT
TGAGATCATTCAGCAAAGACGACAGATTATTGGAACCAAGATCAAAGAAGGGAAGGTGAT
GAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAACAGTGCATTGAGCGGTCAGC
ATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCGTTTCCTACA
GACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAAT
TCCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAA
GAAACTGCTAGAATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGA
GCTCTGCACAGCTTCATATGACACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAG
CGTGGTCTCCTACGAGCTCCAGTACACCATATTCACCGGACAAGCCAACGTCGTTAGTCT
GTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCAAGCAGAACCACTACACGGT
GCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAACCAGGCGGG
CAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCC
CAAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGA
GTCATCATCCAAGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGT
AGCTGGAAATGTGTTTATTGATAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAG
CACATGGTATGCCATTGGTCTTGCTTACAAATCAGCCCCGAAGCATGAATGGATTGGGAA
GAACTCTGCTTCCTGGGCGCTCTGCCGCTGCAACAATAACTGGGTGGTGAGACACAATAG
CAAGGAAATCCCCATTGAGCCTGCCCCCCACCTCCGGCGCGTGGGCATCCTGCTGGACTA
TGATAACGGCTCTATCGCCTTTTATGATGCTTTGAACTCCATCCACCTCTACACCTTCGA
CGTCGCATTTGCGCAGCCTGTTTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACGAT
TATCACTGGGCTCCCTATCCCAGACCATTTGGACTGCACAGAGCAGCTGCCGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT