PI4KA ORF clone pFN21AB1434

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB1434

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Clone info.

Species

human

Clone Name

pFN21AB1434 Sequence (with vector) Restriction Map

Product ID

FHC00322

Flexi Clone PID

FXC00322

Original Clone PID

ORK00322

ORF Sequence

DNA sequence (6306 bp)
Protein sequence (2102 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384703]

Gene

KIBB1434

Symbol

PI4KA

Alias : PI4K-ALPHA, PIK4CA, PMGYCHA, pi4K230

Description

phosphatidylinositol 4-kinase, catalytic, alpha

Original Clone ID

fg01236

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 6306 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 2102 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33603.2 0 100.0 PI4KA CCDS55637.1 1.2e-26 39.3 PI4KB CCDS55638.1 1.7e-26 39.3 PI4KB CCDS81376.1 1.7e-26 39.3 PI4KB CCDS993.1 1.8e-26 39.3 PI4KB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001263 1586 1725 PF00613 Phosphoinositide 3-kinase IPR000403 1846 2050 PF00454 Phosphatidylinositol 3-/4-kinase HMMSmart IPR001263 1541 1729 SM00145 Phosphoinositide 3-kinase IPR000403 1846 2099 SM00146 Phosphatidylinositol 3-/4-kinase ProfileScan NULL 1 19 PS51257 NULL IPR000403 1845 2102 PS50290 Phosphatidylinositol 3-/4-kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_477352 2102 600286,616531 100.0 100.0 XP_016884318 2108 600286,616531 99.0 100.0 XP_005261692 1854 600286,616531 86.8 100.0 XP_005261691 2099 600286,616531 98.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB1434 Download

>KIBB1434  6306 bp
ATGGCGGCGGCCCCGGCCCGGGGAGGCGGAGGCGGAGGCGGAGGCGGCGGCGGCTGCTCC
GGCTCCGGCTCCAGCGCCTCGCGGGGCTTCTATTTCAACACGGTCCTGTCACTGGCCCGC
TCCCTGGCGGTGCAGAGACCAGCATCCTTGGAGAAGGTCCAAAAGCTTCTTTGCATGTGT
CCAGTGGATTTCCATGGGATCTTCCAGTTAGATGAAAGACGGAGAGATGCAGTGATTGCA
TTGGGCATTTTTCTGATTGAATCTGATCTTCAGCACAAAGATTGTGTGGTTCCTTACCTT
CTTCGACTTCTCAAAGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAA
GGCAGAGGTGCCCTCCCGGTTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGTCT
GATGTGGCCTATAGGGATCCTTCACTTAGGGATGAGATTTTAGAGGTGCTTTTGCAGGTT
TTGCATGTCCTCTTGGGGATGTGCCAGGCCTTGGAGATTCAAGACAAAGAATACCTTTGC
AAGTATGCTATCCCATGCCTGATAGGAATCTCGCGAGCATTTGGGCGTTACAGCAACATG
GAAGAGTCTCTCCTCTCAAAGCTCTTTCCCAAAATCCCTCCTCATTCCCTCCGTGTCCTG
GAAGAGCTTGAAGGTGTTCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTCCCCAGC
AATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAAGAGGAAAACCAGCAGTGTGTCCAGC
ATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCCTCCCAGTTCCCCTGGAGGATCTGCC
TTTCACTACTTTGAAGCCTCCTGCTTGCCCGATGGGACTGCCCTAGAGCCTGAGTACTAC
TTTTCAACCATCAGCTCCAGCTTCTCAGTCTCTCCCCTTTTCAACGGTGTCACATATAAG
GAGTTTAACATTCCATTGGAAATGCTTCGGGAACTCTTAAACCTGGTGAAGAAGATCGTT
GAGGAGGCTGTTCTCAAATCTTTGGATGCCATTGTAGCCAGTGTGATGGAGGCCAACCCC
AGTGCTGATCTCTACTACACTTCCTTCAGTGACCCTCTCTACCTGACCATGTTCAAGATG
CTGCGTGACACTCTGTACTACATGAAGGACCTCCCGACCTCTTTTGTGAAGGAGATCCAT
GATTTTGTGCTGGAGCAGTTCAACACGAGCCAGGGGGAGCTCCAGAAGATTCTACATGAC
GCAGACCGGATCCACAATGAGCTGAGCCCCCTCAAACTGCGCTGTCAGGCGAATGCTGCC
TGTGTGGACCTCATGGTGTGGGCTGTGAAGGACGAGCAGGGTGCAGAAAACCTTTGCATC
AAGCTATCTGAGAAGCTGCAGTCCAAGACGTCCAGCAAAGTCATTATTGCTCACTTGCCC
CTGCTGATCTGCTGTCTGCAGGGTTTGGGCCGCCTGTGCGAGAGGTTCCCGGTGGTGGTG
CACTCTGTGACACCGTCCTTGCGAGACTTCCTGGTCATCCCGTCCCCAGTTCTGGTGAAG
CTCTACAAGTACCACAGTCAGTACCACACAGTTGCTGGCAATGATATAAAAATCAGTGTG
ACCAATGAGCATTCCGAGTCAACCCTGAACGTCATGTCGGGTAAGAAGAGCCAGCCCTCC
ATGTACGAGCAGCTCCGAGACATCGCTATTGACAACATCTGCAGGTGCCTGAAGGCTGGA
TTGACGGTGGACCCAGTGATTGTGGAGGCGTTCTTGGCCAGCCTGTCCAACCGGCTCTAC
ATCTCTCAGGAGAGCGACAAGGACGCTCACTTGATTCCCGACCACACAATCCGAGCCTTG
GGACACATTGCGGTGGCCTTGAGGGACACCCCGAAGGTCATGGAGCCCATTCTGCAGATC
CTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCTCGATGTGCTGATTATTGACCAGCTG
GGCTGCCTGGTTATCACCGGAAATCAATACATCTATCAGGAAGTGTGGAACCTCTTCCAG
CAGATCAGTGTGAAGGCCAGCTCCGTTGTATACTCAGCCACCAAAGATTACAAGGACCAC
GGCTATAGGCATTGCTCCCTGGCAGTGATTAATGCCCTGGCCAACATCGCGGCCAACATC
CAAGACGAGCACCTGGTGGATGAGCTGCTCATGAACCTGTTGGAGTTGTTTGTGCAGCTG
GGGCTGGAGGGGAAGCGAGCCAGCGAGAGGGCAAGCGAGAAGGGCCCTGCCCTAAAGGCT
TCTAGCAGTGCAGGGAACTTGGGAGTACTCATTCCTGTAATAGCTGTGCTCACCCGACGA
CTGCCACCCATCAAAGAAGCTAAGCCTCGGTTACAGAAGCTCTTCCGAGACTTCTGGCTG
TATTCCGTTCTGATGGGATTCGCTGTGGAGGGCTCAGGACTCTGGCCAGAAGAATGGTAC
GAGGGGGTCTGTGAAATAGCCACTAAGTCCCCCTTGCTCACCTTTCCCAGCAAGGAGCCA
CTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAAGAATGACACGGTCACCCCCGCTGAG
CTGAGTGAGCTCCGCAGCACTATCATCAACCTGCTGGACCCCCCTCCCGAGGTGTCCGCA
CTCATCAACAAGCTGGACTTCGCCATGTCCACCTACCTCCTCTCTGTGTACCGGCTGGAG
TACATGAGGGTACTGCGTTCAACAGATCCTGATCGCTTCCAGGTAATGTTCTGCTACTTT
GAGGATAAAGCTATTCAGAAAGACAAATCTGGGATGATGCAGTGTGTGATTGCAGTCGCG
GACAAAGTATTCGATGCCTTCCTGAACATGATGGCGGATAAAGCCAAGACCAAGGAGAAC
GAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTTGGTGAACTTCAACCACATCCACAAG
AGGATAAGGAGGGTGGCAGACAAGTATCTATCTGGTCTGGTGGATAAGTTTCCCCACTTG
CTCTGGAGCGGGACTGTGCTGAAGACCATGCTGGACATCCTGCAGACCCTGTCACTGTCA
CTGAGCGCTGATATTCACAAGGATCAGCCTTACTATGACATCCCCGACGCCCCCTACCGG
ATCACGGTTCCTGACACGTACGAAGCCCGTGAGAGCATTGTGAAGGACTTCGCTGCACGC
TGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGCACCTACCGTCACCAAGTCCCACCTG
CAGGAATATCTGAACAAACATCAGAACTGGGTATCGGGACTGTCCCAGCACACGGGGCTG
GCCATGGCCACTGAGAGCATCCTTCACTTTGCTGGCTACAACAAGCAGAACACAACTCTT
GGGGCAACTCAGCTGAGCGAGCGCCCGGCCTGTGTGAAGAAAGACTACTCCAACTTCATG
GCATCCCTGAATCTGCGCAACCGCTACGCGGGCGAGGTGTATGGAATGATTCGGTTCTCA
GGCACCACAGGCCAGATGTCTGACCTGAACAAAATGATGGTCCAGGATCTACATTCAGCT
TTAGACCGCAGTCATCCTCAGCACTACACGCAGGCCATGTTCAAGCTGACCGCAATGCTC
ATTAGCAGTAAAGATTGTGACCCGCAGCTCCTTCATCATCTGTGCTGGGGTCCCCTCCGG
ATGTTCAATGAGCATGGCATGGAGACGGCCCTGGCCTGCTGGGAGTGGCTGCTGGCTGGC
AAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTG
GAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCG
GAAGCAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTACATCTGGATCGAC
TTCCTGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTC
TCCAGCCTGCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAAC
CGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTG
CATGCCGATGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCC
ACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTG
CGTGAAGACATAAGCATCATGATTAAATTTTGGACCGCCATGTTCTCAGATAAGAAGTAC
CTGACCGCCAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGGACATA
ACTGTCGGCTCTCGGCAACAAGCCACCCAAGGCTGGATCAACACATACCCCCTGTCCAGC
GGCATGTCCACCATCTCCAAGAAATCAGGCATGTCTAAGAAAACCAACCGGGGCTCCCAG
CTGCACAAATACTACATGAAGCGCAGGACGCTGCTGCTGTCCCTGCTGGCCACTGAGATC
GAGCGTCTCATCACATGGTACAACCCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCC
GGAGAGAACAGCGTGGCCAACTGGAGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGG
AAGGACAACGTGAACCTCGCCTGGAGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCC
AGGTTTAAGAACACAGAAGCCATTGGGAACGAAGTGACCCGTCTCGTTCGGTTGGACCCG
GGAGCCGTTAGTGATGTGCCTGAAGCAATCAAGTTCCTGGTCACCTGGCACACCATCGAC
GCCGATGCTCCAGAGCTCAGCCATGTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGC
CTCTCCTACTTCTCCAGCATGTACCCGCCGCACCCTCTCACGGCGCAGTACGGGGTGAAA
GTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCC
CTCAGGTACGACAAGATGGGCTATGTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCC
CAGCTTCTGGCACACCAGTTCATCTGGAACATGAAGACTAACATTTATCTAGATGAAGAG
GGCCACCAGAAAGACCCTGACATCGGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACA
GGCTCCTTGTCCGGCCCAGCGAAGGACTTTTACCAGCGGGAGTTTGATTTCTTTAACAAG
ATCACCAACGTGTCGGCTATCATCAAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCT
TGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACCCT
GAGGCCATTGTGCTGGACATCGACTACAAGTCTGGGACCCCGATGCAGAGTGCTGCAAAA
GCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAA
GGTCTGCGGTGCCGCTCAGACTCCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAG
AAGATCTCCTGGCAGGCAGCCATCTTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTG
GCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTCGGCCTGGACCTCTTT
GTTTTTCCCTACCGCGTGGTGGCCACTGCCCCTGGGTGCGGGGTGATCGAGTGCATCCCC
GACTGCACCTCCCGGGACCAGCTGGGCCGCCAGACAGACTTCGGCATGTACGACTACTTC
ACACGCCAGTACGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCATC
CGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCTGCTGCAGATCAAGGACAGACACAAC
GGCAACATTATGCTGGACAAGAAGGGTCATATCATCCACATCGACTTTGGCTTCATGTTT
GAAAGCTCGCCGGGCGGCAATCTCGGCTGGGAACCCGACATCAAGCTGACGGATGAGATG
GTGATGATCATGGGGGGCAAGATGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGT
GTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGACGCGGTCGTCTCCCTGGTCACTCTC
ATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGG
TTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGAGC
TGCTTCCTCAGCAACAGGAGCCGGACCTACGACATGATCCAGTACTATCAGAATGACATC
CCCTAC

ORF Protein

Cloned ORF protein sequence for pFN21AB1434 Download

>KIBB1434  2102 aa
MAAAPARGGGGGGGGGGGCSGSGSSASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMC
PVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARK
GRGALPVAESFSFCLVTLLSDVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQDKEYLC
KYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELEGVRRRSFNDFRSILPS
NLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGGSAFHYFEASCLPDGTALEPEYY
FSTISSSFSVSPLFNGVTYKEFNIPLEMLRELLNLVKKIVEEAVLKSLDAIVASVMEANP
SADLYYTSFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHD
ADRIHNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLP
LLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGNDIKISV
TNEHSESTLNVMSGKKSQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLY
ISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQL
GCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANI
QDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRR
LPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEP
LRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLE
YMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKEN
EEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLS
LSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHL
QEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERPACVKKDYSNFM
ASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLHSALDRSHPQHYTQAMFKLTAML
ISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTV
EQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIF
SSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYS
TAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDI
TVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEI
ERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPA
RFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTG
LSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKS
QLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNK
ITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAK
APYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDML
ALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYF
TRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMF
ESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTL
MLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDI
PY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB1434 Download

>pFN21AB1434  11011 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGCCCCGGCCCGGGGAGGCGGAGGCGG
AGGCGGAGGCGGCGGCGGCTGCTCCGGCTCCGGCTCCAGCGCCTCGCGGGGCTTCTATTT
CAACACGGTCCTGTCACTGGCCCGCTCCCTGGCGGTGCAGAGACCAGCATCCTTGGAGAA
GGTCCAAAAGCTTCTTTGCATGTGTCCAGTGGATTTCCATGGGATCTTCCAGTTAGATGA
AAGACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTGAATCTGATCTTCAGCA
CAAAGATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGTCTTCCAAAAGTGTATTG
GGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTGCCCTCCCGGTTGCAGAGAGCTTCAG
CTTCTGCTTGGTAACTCTGCTGTCTGATGTGGCCTATAGGGATCCTTCACTTAGGGATGA
GATTTTAGAGGTGCTTTTGCAGGTTTTGCATGTCCTCTTGGGGATGTGCCAGGCCTTGGA
GATTCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCCTGATAGGAATCTCGCG
AGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCAAAGCTCTTTCCCAAAAT
CCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCTTGAAGGTGTTCGAAGGCGTTCCTTTAA
TGACTTCCGCTCCATCCTCCCCAGCAATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAA
GAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCC
TCCCAGTTCCCCTGGAGGATCTGCCTTTCACTACTTTGAAGCCTCCTGCTTGCCCGATGG
GACTGCCCTAGAGCCTGAGTACTACTTTTCAACCATCAGCTCCAGCTTCTCAGTCTCTCC
CCTTTTCAACGGTGTCACATATAAGGAGTTTAACATTCCATTGGAAATGCTTCGGGAACT
CTTAAACCTGGTGAAGAAGATCGTTGAGGAGGCTGTTCTCAAATCTTTGGATGCCATTGT
AGCCAGTGTGATGGAGGCCAACCCCAGTGCTGATCTCTACTACACTTCCTTCAGTGACCC
TCTCTACCTGACCATGTTCAAGATGCTGCGTGACACTCTGTACTACATGAAGGACCTCCC
GACCTCTTTTGTGAAGGAGATCCATGATTTTGTGCTGGAGCAGTTCAACACGAGCCAGGG
GGAGCTCCAGAAGATTCTACATGACGCAGACCGGATCCACAATGAGCTGAGCCCCCTCAA
ACTGCGCTGTCAGGCGAATGCTGCCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGACGA
GCAGGGTGCAGAAAACCTTTGCATCAAGCTATCTGAGAAGCTGCAGTCCAAGACGTCCAG
CAAAGTCATTATTGCTCACTTGCCCCTGCTGATCTGCTGTCTGCAGGGTTTGGGCCGCCT
GTGCGAGAGGTTCCCGGTGGTGGTGCACTCTGTGACACCGTCCTTGCGAGACTTCCTGGT
CATCCCGTCCCCAGTTCTGGTGAAGCTCTACAAGTACCACAGTCAGTACCACACAGTTGC
TGGCAATGATATAAAAATCAGTGTGACCAATGAGCATTCCGAGTCAACCCTGAACGTCAT
GTCGGGTAAGAAGAGCCAGCCCTCCATGTACGAGCAGCTCCGAGACATCGCTATTGACAA
CATCTGCAGGTGCCTGAAGGCTGGATTGACGGTGGACCCAGTGATTGTGGAGGCGTTCTT
GGCCAGCCTGTCCAACCGGCTCTACATCTCTCAGGAGAGCGACAAGGACGCTCACTTGAT
TCCCGACCACACAATCCGAGCCTTGGGACACATTGCGGTGGCCTTGAGGGACACCCCGAA
GGTCATGGAGCCCATTCTGCAGATCCTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCT
CGATGTGCTGATTATTGACCAGCTGGGCTGCCTGGTTATCACCGGAAATCAATACATCTA
TCAGGAAGTGTGGAACCTCTTCCAGCAGATCAGTGTGAAGGCCAGCTCCGTTGTATACTC
AGCCACCAAAGATTACAAGGACCACGGCTATAGGCATTGCTCCCTGGCAGTGATTAATGC
CCTGGCCAACATCGCGGCCAACATCCAAGACGAGCACCTGGTGGATGAGCTGCTCATGAA
CCTGTTGGAGTTGTTTGTGCAGCTGGGGCTGGAGGGGAAGCGAGCCAGCGAGAGGGCAAG
CGAGAAGGGCCCTGCCCTAAAGGCTTCTAGCAGTGCAGGGAACTTGGGAGTACTCATTCC
TGTAATAGCTGTGCTCACCCGACGACTGCCACCCATCAAAGAAGCTAAGCCTCGGTTACA
GAAGCTCTTCCGAGACTTCTGGCTGTATTCCGTTCTGATGGGATTCGCTGTGGAGGGCTC
AGGACTCTGGCCAGAAGAATGGTACGAGGGGGTCTGTGAAATAGCCACTAAGTCCCCCTT
GCTCACCTTTCCCAGCAAGGAGCCACTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAA
GAATGACACGGTCACCCCCGCTGAGCTGAGTGAGCTCCGCAGCACTATCATCAACCTGCT
GGACCCCCCTCCCGAGGTGTCCGCACTCATCAACAAGCTGGACTTCGCCATGTCCACCTA
CCTCCTCTCTGTGTACCGGCTGGAGTACATGAGGGTACTGCGTTCAACAGATCCTGATCG
CTTCCAGGTAATGTTCTGCTACTTTGAGGATAAAGCTATTCAGAAAGACAAATCTGGGAT
GATGCAGTGTGTGATTGCAGTCGCGGACAAAGTATTCGATGCCTTCCTGAACATGATGGC
GGATAAAGCCAAGACCAAGGAGAACGAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTT
GGTGAACTTCAACCACATCCACAAGAGGATAAGGAGGGTGGCAGACAAGTATCTATCTGG
TCTGGTGGATAAGTTTCCCCACTTGCTCTGGAGCGGGACTGTGCTGAAGACCATGCTGGA
CATCCTGCAGACCCTGTCACTGTCACTGAGCGCTGATATTCACAAGGATCAGCCTTACTA
TGACATCCCCGACGCCCCCTACCGGATCACGGTTCCTGACACGTACGAAGCCCGTGAGAG
CATTGTGAAGGACTTCGCTGCACGCTGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGC
ACCTACCGTCACCAAGTCCCACCTGCAGGAATATCTGAACAAACATCAGAACTGGGTATC
GGGACTGTCCCAGCACACGGGGCTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCTGG
CTACAACAAGCAGAACACAACTCTTGGGGCAACTCAGCTGAGCGAGCGCCCGGCCTGTGT
GAAGAAAGACTACTCCAACTTCATGGCATCCCTGAATCTGCGCAACCGCTACGCGGGCGA
GGTGTATGGAATGATTCGGTTCTCAGGCACCACAGGCCAGATGTCTGACCTGAACAAAAT
GATGGTCCAGGATCTACATTCAGCTTTAGACCGCAGTCATCCTCAGCACTACACGCAGGC
CATGTTCAAGCTGACCGCAATGCTCATTAGCAGTAAAGATTGTGACCCGCAGCTCCTTCA
TCATCTGTGCTGGGGTCCCCTCCGGATGTTCAATGAGCATGGCATGGAGACGGCCCTGGC
CTGCTGGGAGTGGCTGCTGGCTGGCAAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGAT
GGCAGGGGCCTGGCACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAA
GGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGTCCCCCCGAAGT
GACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATCGCCAAGTACTG
CAGCTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCCTGAACAT
CGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCT
GCTGACCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCAACCATCCGCAA
TGTGCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTT
CCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATCATGATTAAATTTTGGAC
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCCCAGATAATCA
GGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAACAAGCCACCCAAGGCTG
GATCAACACATACCCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGCATGTC
TAAGAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGCAGGACGCTGCT
GCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGGTACAACCCGCTGTCAGC
CCCGGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGGAGATCTAAGTA
CATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTCGCCTGGAGCATCTCTCC
CTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATTGGGAACGAAGT
GACCCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAAGCAATCAAGTT
CCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTGTGCTG
GGCGCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTACCCGCCGCACCC
TCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTT
CTACATCCCCCAGATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTATGTGCGGGAGTA
TATTCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATCTGGAACATGAA
GACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCTGACATCGGCGACCTCCT
GGATCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAGGACTTTTACCA
GCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCTATCATCAAGCCCTACCC
TAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCC
GGGCTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGACTACAAGTCTGG
GACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATG
TGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCCGAGGATGAGTG
CAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCAGCCATCTTCAAGGTGGG
AGACGACTGCCGGCAGGACATGCTGGCCCTGCAGATCATCGACCTCTTCAAGAACATCTT
CCAGCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTGCCCCTGG
GTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTGGGCCGCCAGAC
AGACTTCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCCACTCTGGCCTT
CCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCT
GCTGCAGATCAAGGACAGACACAACGGCAACATTATGCTGGACAAGAAGGGTCATATCAT
CCACATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTCGGCTGGGAACC
CGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGCAAGATGGAGGCCACACC
CTTCAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGA
CGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCA
GACAATCAAGCTCTTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAA
TTTCATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGGACCTACGACAT
GATCCAGTACTATCAGAATGACATCCCCTACGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT