MYH7B ORF clone pFN21AA1512

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AA1512

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Clone info.

Species

human

Clone Name

pFN21AA1512 Sequence (with vector) Restriction Map

Product ID

FHC06072

Flexi Clone PID

FXC06072

ORF Sequence

DNA sequence (5949 bp)
Protein sequence (1983 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB463134]

Gene

KIAA1512

Symbol

MYH7B

Alias : MHC14, MYH14

Description

myosin, heavy chain 7B, cardiac muscle, beta

Original Clone ID

fg04660y1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5949 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1983 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42869.1 0 100.0 MYH7B CCDS9600.1 2.8e-186 68.5 MYH6 CCDS9601.1 7.9e-186 69.5 MYH7 CCDS11156.1 5.7e-183 67.8 MYH2 CCDS11155.1 1.7e-182 67.5 MYH1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001609 156 175 PR00193 Myosin head IPR001609 212 237 PR00193 Myosin head IPR001609 270 297 PR00193 Myosin head IPR001609 501 529 PR00193 Myosin head IPR001609 555 583 PR00193 Myosin head HMMPfam IPR004009 74 114 PF02736 Myosin IPR001609 129 815 PF00063 Myosin head IPR002928 1119 1974 PF01576 Myosin tail HMMSmart IPR001609 120 828 SM00242 Myosin head ProfileScan IPR000048 830 859 PS50096 IQ motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065935 1983 609928 100.0 100.0 XP_006723903 1989 609928 100.0 99.8 XP_011527243 1997 609928 97.1 99.8 XP_016883475 2003 609928 99.0 100.0 XP_011527250 1738 609928 98.8 86.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1512 Download

>KIAA1512  5949 bp
ATGAGTGGCAATAAAAGGGGTAGCAGAGCTTCCTGCCCTCACCGTGGTGCCGAGTGCCTG
CTGCCTTGGGCCGCCTTGAACCTCCAGGGTTTCCAGCTCCTCCTCCTTCACCCCAGTGCC
ACTGCCATGATGGATGTGAGTGAACTTGGGGAGTCTGCCCGCTACCTCCGCCAGGGCTAC
CAGGAGATGACGAAGGTGCACACTATCCCATGGGACGGGAAGAAGCGAGTCTGGGTGCCT
GATGAACAGGACGCCTACGTGGAGGCCGAGGTCAAGTCGGAGGCTACCGGGGGCAGAGTC
ACCGTGGAGACCAAAGACCAGAAGGTGCTGATGGTGCGTGAAGCCGAGCTGCAGCCCATG
AACCCGCCTCGCTTCGACTTACTGGAGGACATGGCCATGATGACGCACCTGAACGAGGCC
TCTGTGCTGCACAACCTGCGCCAGCGCTATGCCCGCTGGATGATCTATACCTACTCAGGC
CTCTTCTGTGTCACCATCAACCCCTACAAATGGCTCCCAGTCTATACGGCCTCCGTAGTG
GCTGCTTACAAGGGAAAGCGCCGCTCAGATTCCCCGCCCCATATATATGCGGTGGCGGAC
AACGCCTACAACGACATGCTGCGCAACCGAGACAACCAGTCCATGCTGATCACCGGAGAG
TCGGGGGCCGGTAAGACGGTTAACACCAAGCGGGTCATTCAGTACTTTGCCATCGTCGCT
GCCCTGGGAGACGGGCCGGGCAAGAAGGCCCAATTTCTGGCAACAAAGACGGGGGGCACC
CTTGAGGATCAAATCATCGAGGCCAACCCTGCCATGGAGGCCTTTGGCAACGCCAAGACC
CTGAGGAATGATAACTCCTCCCGCTTTGGCAAGTTCATCCGCATTCACTTTGGTCCCTCT
GGGAAGCTGGCATCCGCGGATATTGACAGCTATCTCCTGGAGAAGTCGCGGGTGATCTTC
CAGTTGCCTGGTGAGCGCAGCTACCATGTCTACTACCAGATCCTCTCAGGGAGGAAGCCA
GAGCTGCAGGACATGCTGCTTCTGTCTATGAACCCCTATGACTACCACTTCTGCAGCCAG
GGCGTCATCACCGTGGACAACATGAATGATGGGGAGGAGCTCATCGCCACCGACCATGCC
ATGGACATCCTAGGCTTCAGCGTGGATGAGAAATGTGCCTGCTATAAGATCGTGGGCGCC
CTCCTGCACTTTGGCAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGGAGGCC
GATGGCACTGAGAGTGCTGACAAGGCTGCCTACCTGATGGGGGTCAGCAGTGGGGACCTC
CTCAAAGGCCTTTTGCACCCCCGGGTGCGTGTAGGGAACGAGTACGTGACCAAGGGCCAG
AGTGTGGAGCAGGTGGTGTTTGCTGTGGGGGCTCTGGCCAAGGCCACCTATGACCGGCTG
TTCAGGTGGCTGGTGTCTCGGATCAACCAGACCCTGGACACAAAGCTGCCCCGGCAGTTC
TTCATCGGGGTTCTGGACATCGCTGGGTTTGAGATCTTTGAGTTCAACAGCTTCGAACAG
CTGTGCATCAACTTCACCAATGAGAAATTGCAGCAGTTCTTCAACCAGCACATGTTTGTG
CTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGACTGGGTCTTCATCGACTTCGGCCTT
GACCTGCAGCCTTGCATCGACCTCATCGAGAAGCCACTGGGCATCCTGTCCATCCTGGAG
GAGGAATGCATGTTCCCCAAGGCCTCAGACGCCAGCTTCCGGGCCAAGCTCTACGACAAC
CACGCGGGGAAGTCACCCAATTTCCAGCAGCCTCGGCCTGACAAGAAGCGCAAGTACCAG
GCCCACTTCGAGGTGGTCCACTACGCAGGCGTGGTGCCTTACAGCATTGTGGGCTGGCTG
GAGAAAAACAAGGATCCCCTGAATGAGACCGTGGTCCCCATCTTCCAGAAGTCACAGAAT
AGGCTCCTGGCGACTCTCTATGAGAATTATGCGGGCTCCTGCTCCACTGAGCCCCCCAAG
TCTGGGGTGAAAGAGAAGCGTAAGAAGGCAGCATCGTTCCAGACGGTGTCCCAGCTGCAC
AAGGAGAACCTCAACAAGCTGATGACCAACCTGCGGGCCACACAGCCCCACTTCGTCCGC
TGCATTGTCCCCAACGAGAACAAAACCCCAGGGGTCATGGATGCCTTCTTGGTGCTACAC
CAGCTGCGCTGCAATGGGGTCCTGGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCCAAC
AGGCTGCTCTACACCGACTTCCGGCAGCGGTACCGTATCCTGAACCCCAGTGCCATCCCG
GATGACACCTTCATGGACAGCAGGAAGGCCACAGAGAAACTGCTGGGCTCGCTGGACTTG
GATCACACCCAGTACCAGTTTGGCCACACCAAGGTGTTCTTCAAGGCTGGGCTTCTAGGC
GTCCTGGAAGAGCTCCGTGACCAGCGCCTGGCCAAGGTGCTGACGCTGCTGCAGGCGCGG
AGCCGTGGCCGCCTCATGCGCCTTGAGTACCAGCGCCTGCTGGGAGGCAGGGATGCACTG
TTCACCATCCAGTGGAACATCCGTGCCTTCAATGCCGTCAAGAACTGGTCATGGATGAAG
CTCTTTTTCAAGATGAAGCCGCTGCTGCGCTCGGCGCAGGCTGAGGAGGAGCTGGCGGCC
CTGCGGGCAGAGCTGCGGGGGTTGCGAGGGGCGCTGGCTGCGGCCGAGGCCAAGCGCCAG
GAACTGGAGGAGACGCACGTCAGCATCACCCAGGAGAAGAATGACCTGGCCCTGCAGCTG
CAGGCTGAGCAGGACAACCTGGCAGATGCCGAGGAGCGCTGCCACTTGCTGATCAAGTCC
AAGGTGCAGCTGGAGGGGAAGGTGAAGGAGCTGAGTGAGCGGCTGGAGGATGAGGAGGAG
GTGAACGCTGACCTGGCCGCCCGCCGGCGCAAGCTGGAGGACGAGTGCACGGAGCTCAAG
AAGGACATTGATGACCTGGAGCTGACACTGGCCAAAGCTGAGAAGGAGAAGCAAGCCACT
GAGAACAAGGTGAAGAACCTGACGGAAGAGATGGCTGCGCTGGACGAGTCAGTGGCCCGG
CTGACCAAGGAGAAGAAGGCGTTGCAGGAGGCCCACCAACAGGCCCTGGGTGACCTGCAG
GCCGAGGAGGACCGTGTGAGCGCGCTGACTAAGGCCAAGCTCCGGCTGGAGCAACAGGTG
GAGGACCTGGAATGCTCCCTGGAGCAGGAGAAGAAGCTGCGCATGGACACGGAGCGGGCC
AAGCGCAAGCTGGAGGGTGACCTGAAGCTGACGCAGGAGTCGGTGGCTGATGCTGCTCAA
GACAAGCAGCAGCTGGAGGAGAAGCTCAAGAAGAAGGACTCCGAGCTGAGCCAGCTGAGC
CTGCGGGTGGAAGACGAGCAGCTCTTGGGGGCCCAGATGCAGAAGAAGATCAAGGAGCTG
CAGGCTCGGGCGGAGGAGCTGGAAGAGGAGCTGGAGGCAGAGCGGGCAGCCCGGGCCCGC
GTGGAGAAGCAGCGTGCAGAGGCGGCGCGGGAGCTGGAGGAGCTGAGCGAGCGGCTGGAG
GAGGCAGGCGGCGCATCCGCGGGGCAGCGCGAGGGCTGCCGCAAGCGGGAGGCGGAGCTG
GGGAGGCTGCGGCGGGAGCTGGAGGAGGCGGCGCTGCGGCACGAGGCCACAGTGGCGGCA
CTGCGGCGCAAGCAGGCGGAGGGCGCGGCGGAGCTGGGGGAGCAGGTGGACAGCCTGCAG
CGGGTGCGGCAGAAGCTGGAGAAGGAGAAGAGTGAGCTGCGCATGGAGGTGGACGACCTG
GCTGCCAACGTGGAGACTCTGACCCGCGCCAAGGCCAGTGCAGAGAAGCTGTGCCGGACC
TATGAGGATCAGCTAAGCGAGGCCAAGATCAAGGTGGAGGAGCTGCAGCGGCAGCTGGCG
GACGCAAGCACGCAGCGTGGGCGACTACAGACGGAAAGCGGGGAGCTGAGTCGCCTGCTA
GAGGAGAAGGAGTGTCTGATCAGTCAGCTGAGCCGTGGAAAGGCCCTGGCCGCCCAAAGC
CTGGAAGAGTTGCGGCGCCAGCTAGAGGAGGAAAGCAAGGCCAAGAGTGCCCTGGCCCAC
GCCGTGCAGGCTCTGCGGCACGACTGTGACCTCCTGCGGGAGCAACACGAGGAGGAGGCT
GAGGCCCAGGCTGAGCTGCAGCGGCTGCTGTCCAAGGCCAATGCCGAGGTGGCCCAGTGG
AGGAGCAAGTACGAAGCAGATGCCATCCAGAGGACCGAGGAGCTGGAGGAGGCCAAAAAA
AAGCTGGCACTGCGGCTGCAGGAGGCAGAGGAGGGCGTGGAGGCTGCCAACGCCAAGTGC
TCATCGTTGGAGAAGGCCAAGCTGCGGCTACAGACAGAGTCAGAGGATGTAACCCTGGAG
CTGGAGCGGGCGACCTCAGCAGCTGCTGCGCTGGACAAGAAGCAGCGGCACTTGGAACGG
GCACTGGAGGAACGGCGGCGGCAGGAGGAGGAGATGCAGCGGGAGCTGGAGGCGGCACAG
AGGGAGTCCCGTGGCCTGGGCACCGAGCTCTTCCGGCTGCGGCACGGCCACGAGGAGGCA
CTTGAAGCCCTGGAGACGCTCAAGCGGGAGAACAAGAACCTGCAGGAGGAGATCAGCGAC
CTCACAGACCAGGTGAGTCTCAGTGGGAAGAGCATCCAGGAACTGGAGAAAACCAAGAAG
GCGCTGGAAGGCGAGAAGAGTGAGATCCAGGCTGCACTGGAGGAGGCAGAGGGGGCCCTG
GAGCTGGAGGAGACCAAGACGCTGCGGATCCAGCTGGAGCTCTCCCAGGTCAAAGCAGAA
GTGGACCGGAAGCTGGCAGAGAAAGACGAGGAGTGCGCTAACCTGAGGCGCAACCACCAG
CGAGCTGTGGAGTCCCTGCAGGCCTCCCTGGATGCAGAGACACGGGCCCGCAATGAGGCG
CTGCGGCTCAAGAAGAAGATGGAGGGTGACCTCAACGACCTGGAGCTGCAGCTGGGCCAT
GCCACCCGTCAGGCCACAGAGGCCCAGGCTGCCACGCGGCTGATGCAGGCACAGCTCAAG
GAGGAGCAGGCAGGGCGGGACGAGGAGCAGCGGCTGGCAGCTGAGCTCCACGAGCAGGCG
CAGGCTCTGGAGCGCCGGGCCTCGCTGCTGGCTGCGGAGCTGGAGGAGCTGCGGGCTGCC
CTGGAGCAGGGCGAGCGCAGCCGGCGACTGGCAGAGCAGGAGCTTTTGGAGGCCACCGAG
CGCCTCAACCTTCTGCATTCGCAGAACACAGGCCTCCTAAACCAGAAGAAGAAGCTGGAG
GCGGACTTGGCCCAGCTGAGCGGGGAGGTGGAGGAGGCTGCACAGGAGAGGCGGGAGGCT
GAGGAGAAGGCCAAAAAGGCCATCACTGATGCGGCCATGATGGCCGAGGAGCTGAAGAAG
GAGCAGGACACAAGTGCACACCTGGAACGGATGAAGAAGACGCTGGAGCAGACGGTGCGC
GAGCTCCAGGCCCGCCTTGAGGAGGCAGAACAGGCCGCCCTCCGTGGCGGGAAGAAGCAG
GTGCAGAAGCTGGAGGCCAAGGTACGGGAGCTGGAGGCTGAGCTTGATGCAGAGCAGAAG
AAGCACGCCGAGGCCCTTAAGGGCGTGCGCAAGCATGAGCGCCGTGTCAAGGAGCTCGCA
TACCAGGCCGAGGAGGACAGGAAGAACCTGGCTCGCATGCAGGACCTGGTGGACAAGCTG
CAGAGCAAGGTCAAGAGCTACAAGCGCCAGTTTGAGGAGGCGGAGCAGCAGGCCAACACC
AACCTGGCCAAGTATCGCAAGGCCCAGCACGAGCTGGATGATGCGGAGGAGCGGGCAGAC
ATGGCGGAAACCCAGGCCAACAAGCTGCGGGCACGGACCCGGGACGCCCTGGGCCCCAAG
CACAAGGAG

ORF Protein

Cloned ORF protein sequence for pFN21AA1512 Download

>KIAA1512  1983 aa
MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGY
QEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPM
NPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVV
AAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVA
ALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPS
GKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQ
GVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEA
DGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRL
FRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFV
LEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDN
HAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQN
RLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVR
CIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIP
DDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQAR
SRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAA
LRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKS
KVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQAT
ENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQV
EDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLS
LRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLE
EAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQ
RVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLA
DASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAH
AVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKK
KLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLER
ALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISD
LTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAE
VDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGH
ATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAA
LEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREA
EEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQ
VQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKL
QSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPK
HKE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1512 Download

>pFN21AA1512  10654 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGGCAATAAAAGGGGTAGCAGAGCTTCCTG
CCCTCACCGTGGTGCCGAGTGCCTGCTGCCTTGGGCCGCCTTGAACCTCCAGGGTTTCCA
GCTCCTCCTCCTTCACCCCAGTGCCACTGCCATGATGGATGTGAGTGAACTTGGGGAGTC
TGCCCGCTACCTCCGCCAGGGCTACCAGGAGATGACGAAGGTGCACACTATCCCATGGGA
CGGGAAGAAGCGAGTCTGGGTGCCTGATGAACAGGACGCCTACGTGGAGGCCGAGGTCAA
GTCGGAGGCTACCGGGGGCAGAGTCACCGTGGAGACCAAAGACCAGAAGGTGCTGATGGT
GCGTGAAGCCGAGCTGCAGCCCATGAACCCGCCTCGCTTCGACTTACTGGAGGACATGGC
CATGATGACGCACCTGAACGAGGCCTCTGTGCTGCACAACCTGCGCCAGCGCTATGCCCG
CTGGATGATCTATACCTACTCAGGCCTCTTCTGTGTCACCATCAACCCCTACAAATGGCT
CCCAGTCTATACGGCCTCCGTAGTGGCTGCTTACAAGGGAAAGCGCCGCTCAGATTCCCC
GCCCCATATATATGCGGTGGCGGACAACGCCTACAACGACATGCTGCGCAACCGAGACAA
CCAGTCCATGCTGATCACCGGAGAGTCGGGGGCCGGTAAGACGGTTAACACCAAGCGGGT
CATTCAGTACTTTGCCATCGTCGCTGCCCTGGGAGACGGGCCGGGCAAGAAGGCCCAATT
TCTGGCAACAAAGACGGGGGGCACCCTTGAGGATCAAATCATCGAGGCCAACCCTGCCAT
GGAGGCCTTTGGCAACGCCAAGACCCTGAGGAATGATAACTCCTCCCGCTTTGGCAAGTT
CATCCGCATTCACTTTGGTCCCTCTGGGAAGCTGGCATCCGCGGATATTGACAGCTATCT
CCTGGAGAAGTCGCGGGTGATCTTCCAGTTGCCTGGTGAGCGCAGCTACCATGTCTACTA
CCAGATCCTCTCAGGGAGGAAGCCAGAGCTGCAGGACATGCTGCTTCTGTCTATGAACCC
CTATGACTACCACTTCTGCAGCCAGGGCGTCATCACCGTGGACAACATGAATGATGGGGA
GGAGCTCATCGCCACCGACCATGCCATGGACATCCTAGGCTTCAGCGTGGATGAGAAATG
TGCCTGCTATAAGATCGTGGGCGCCCTCCTGCACTTTGGCAACATGAAGTTCAAGCAGAA
GCAGCGGGAGGAGCAGGCGGAGGCCGATGGCACTGAGAGTGCTGACAAGGCTGCCTACCT
GATGGGGGTCAGCAGTGGGGACCTCCTCAAAGGCCTTTTGCACCCCCGGGTGCGTGTAGG
GAACGAGTACGTGACCAAGGGCCAGAGTGTGGAGCAGGTGGTGTTTGCTGTGGGGGCTCT
GGCCAAGGCCACCTATGACCGGCTGTTCAGGTGGCTGGTGTCTCGGATCAACCAGACCCT
GGACACAAAGCTGCCCCGGCAGTTCTTCATCGGGGTTCTGGACATCGCTGGGTTTGAGAT
CTTTGAGTTCAACAGCTTCGAACAGCTGTGCATCAACTTCACCAATGAGAAATTGCAGCA
GTTCTTCAACCAGCACATGTTTGTGCTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGA
CTGGGTCTTCATCGACTTCGGCCTTGACCTGCAGCCTTGCATCGACCTCATCGAGAAGCC
ACTGGGCATCCTGTCCATCCTGGAGGAGGAATGCATGTTCCCCAAGGCCTCAGACGCCAG
CTTCCGGGCCAAGCTCTACGACAACCACGCGGGGAAGTCACCCAATTTCCAGCAGCCTCG
GCCTGACAAGAAGCGCAAGTACCAGGCCCACTTCGAGGTGGTCCACTACGCAGGCGTGGT
GCCTTACAGCATTGTGGGCTGGCTGGAGAAAAACAAGGATCCCCTGAATGAGACCGTGGT
CCCCATCTTCCAGAAGTCACAGAATAGGCTCCTGGCGACTCTCTATGAGAATTATGCGGG
CTCCTGCTCCACTGAGCCCCCCAAGTCTGGGGTGAAAGAGAAGCGTAAGAAGGCAGCATC
GTTCCAGACGGTGTCCCAGCTGCACAAGGAGAACCTCAACAAGCTGATGACCAACCTGCG
GGCCACACAGCCCCACTTCGTCCGCTGCATTGTCCCCAACGAGAACAAAACCCCAGGGGT
CATGGATGCCTTCTTGGTGCTACACCAGCTGCGCTGCAATGGGGTCCTGGAGGGGATCCG
GATCTGCCGCCAAGGGTTCCCCAACAGGCTGCTCTACACCGACTTCCGGCAGCGGTACCG
TATCCTGAACCCCAGTGCCATCCCGGATGACACCTTCATGGACAGCAGGAAGGCCACAGA
GAAACTGCTGGGCTCGCTGGACTTGGATCACACCCAGTACCAGTTTGGCCACACCAAGGT
GTTCTTCAAGGCTGGGCTTCTAGGCGTCCTGGAAGAGCTCCGTGACCAGCGCCTGGCCAA
GGTGCTGACGCTGCTGCAGGCGCGGAGCCGTGGCCGCCTCATGCGCCTTGAGTACCAGCG
CCTGCTGGGAGGCAGGGATGCACTGTTCACCATCCAGTGGAACATCCGTGCCTTCAATGC
CGTCAAGAACTGGTCATGGATGAAGCTCTTTTTCAAGATGAAGCCGCTGCTGCGCTCGGC
GCAGGCTGAGGAGGAGCTGGCGGCCCTGCGGGCAGAGCTGCGGGGGTTGCGAGGGGCGCT
GGCTGCGGCCGAGGCCAAGCGCCAGGAACTGGAGGAGACGCACGTCAGCATCACCCAGGA
GAAGAATGACCTGGCCCTGCAGCTGCAGGCTGAGCAGGACAACCTGGCAGATGCCGAGGA
GCGCTGCCACTTGCTGATCAAGTCCAAGGTGCAGCTGGAGGGGAAGGTGAAGGAGCTGAG
TGAGCGGCTGGAGGATGAGGAGGAGGTGAACGCTGACCTGGCCGCCCGCCGGCGCAAGCT
GGAGGACGAGTGCACGGAGCTCAAGAAGGACATTGATGACCTGGAGCTGACACTGGCCAA
AGCTGAGAAGGAGAAGCAAGCCACTGAGAACAAGGTGAAGAACCTGACGGAAGAGATGGC
TGCGCTGGACGAGTCAGTGGCCCGGCTGACCAAGGAGAAGAAGGCGTTGCAGGAGGCCCA
CCAACAGGCCCTGGGTGACCTGCAGGCCGAGGAGGACCGTGTGAGCGCGCTGACTAAGGC
CAAGCTCCGGCTGGAGCAACAGGTGGAGGACCTGGAATGCTCCCTGGAGCAGGAGAAGAA
GCTGCGCATGGACACGGAGCGGGCCAAGCGCAAGCTGGAGGGTGACCTGAAGCTGACGCA
GGAGTCGGTGGCTGATGCTGCTCAAGACAAGCAGCAGCTGGAGGAGAAGCTCAAGAAGAA
GGACTCCGAGCTGAGCCAGCTGAGCCTGCGGGTGGAAGACGAGCAGCTCTTGGGGGCCCA
GATGCAGAAGAAGATCAAGGAGCTGCAGGCTCGGGCGGAGGAGCTGGAAGAGGAGCTGGA
GGCAGAGCGGGCAGCCCGGGCCCGCGTGGAGAAGCAGCGTGCAGAGGCGGCGCGGGAGCT
GGAGGAGCTGAGCGAGCGGCTGGAGGAGGCAGGCGGCGCATCCGCGGGGCAGCGCGAGGG
CTGCCGCAAGCGGGAGGCGGAGCTGGGGAGGCTGCGGCGGGAGCTGGAGGAGGCGGCGCT
GCGGCACGAGGCCACAGTGGCGGCACTGCGGCGCAAGCAGGCGGAGGGCGCGGCGGAGCT
GGGGGAGCAGGTGGACAGCCTGCAGCGGGTGCGGCAGAAGCTGGAGAAGGAGAAGAGTGA
GCTGCGCATGGAGGTGGACGACCTGGCTGCCAACGTGGAGACTCTGACCCGCGCCAAGGC
CAGTGCAGAGAAGCTGTGCCGGACCTATGAGGATCAGCTAAGCGAGGCCAAGATCAAGGT
GGAGGAGCTGCAGCGGCAGCTGGCGGACGCAAGCACGCAGCGTGGGCGACTACAGACGGA
AAGCGGGGAGCTGAGTCGCCTGCTAGAGGAGAAGGAGTGTCTGATCAGTCAGCTGAGCCG
TGGAAAGGCCCTGGCCGCCCAAAGCCTGGAAGAGTTGCGGCGCCAGCTAGAGGAGGAAAG
CAAGGCCAAGAGTGCCCTGGCCCACGCCGTGCAGGCTCTGCGGCACGACTGTGACCTCCT
GCGGGAGCAACACGAGGAGGAGGCTGAGGCCCAGGCTGAGCTGCAGCGGCTGCTGTCCAA
GGCCAATGCCGAGGTGGCCCAGTGGAGGAGCAAGTACGAAGCAGATGCCATCCAGAGGAC
CGAGGAGCTGGAGGAGGCCAAAAAAAAGCTGGCACTGCGGCTGCAGGAGGCAGAGGAGGG
CGTGGAGGCTGCCAACGCCAAGTGCTCATCGTTGGAGAAGGCCAAGCTGCGGCTACAGAC
AGAGTCAGAGGATGTAACCCTGGAGCTGGAGCGGGCGACCTCAGCAGCTGCTGCGCTGGA
CAAGAAGCAGCGGCACTTGGAACGGGCACTGGAGGAACGGCGGCGGCAGGAGGAGGAGAT
GCAGCGGGAGCTGGAGGCGGCACAGAGGGAGTCCCGTGGCCTGGGCACCGAGCTCTTCCG
GCTGCGGCACGGCCACGAGGAGGCACTTGAAGCCCTGGAGACGCTCAAGCGGGAGAACAA
GAACCTGCAGGAGGAGATCAGCGACCTCACAGACCAGGTGAGTCTCAGTGGGAAGAGCAT
CCAGGAACTGGAGAAAACCAAGAAGGCGCTGGAAGGCGAGAAGAGTGAGATCCAGGCTGC
ACTGGAGGAGGCAGAGGGGGCCCTGGAGCTGGAGGAGACCAAGACGCTGCGGATCCAGCT
GGAGCTCTCCCAGGTCAAAGCAGAAGTGGACCGGAAGCTGGCAGAGAAAGACGAGGAGTG
CGCTAACCTGAGGCGCAACCACCAGCGAGCTGTGGAGTCCCTGCAGGCCTCCCTGGATGC
AGAGACACGGGCCCGCAATGAGGCGCTGCGGCTCAAGAAGAAGATGGAGGGTGACCTCAA
CGACCTGGAGCTGCAGCTGGGCCATGCCACCCGTCAGGCCACAGAGGCCCAGGCTGCCAC
GCGGCTGATGCAGGCACAGCTCAAGGAGGAGCAGGCAGGGCGGGACGAGGAGCAGCGGCT
GGCAGCTGAGCTCCACGAGCAGGCGCAGGCTCTGGAGCGCCGGGCCTCGCTGCTGGCTGC
GGAGCTGGAGGAGCTGCGGGCTGCCCTGGAGCAGGGCGAGCGCAGCCGGCGACTGGCAGA
GCAGGAGCTTTTGGAGGCCACCGAGCGCCTCAACCTTCTGCATTCGCAGAACACAGGCCT
CCTAAACCAGAAGAAGAAGCTGGAGGCGGACTTGGCCCAGCTGAGCGGGGAGGTGGAGGA
GGCTGCACAGGAGAGGCGGGAGGCTGAGGAGAAGGCCAAAAAGGCCATCACTGATGCGGC
CATGATGGCCGAGGAGCTGAAGAAGGAGCAGGACACAAGTGCACACCTGGAACGGATGAA
GAAGACGCTGGAGCAGACGGTGCGCGAGCTCCAGGCCCGCCTTGAGGAGGCAGAACAGGC
CGCCCTCCGTGGCGGGAAGAAGCAGGTGCAGAAGCTGGAGGCCAAGGTACGGGAGCTGGA
GGCTGAGCTTGATGCAGAGCAGAAGAAGCACGCCGAGGCCCTTAAGGGCGTGCGCAAGCA
TGAGCGCCGTGTCAAGGAGCTCGCATACCAGGCCGAGGAGGACAGGAAGAACCTGGCTCG
CATGCAGGACCTGGTGGACAAGCTGCAGAGCAAGGTCAAGAGCTACAAGCGCCAGTTTGA
GGAGGCGGAGCAGCAGGCCAACACCAACCTGGCCAAGTATCGCAAGGCCCAGCACGAGCT
GGATGATGCGGAGGAGCGGGCAGACATGGCGGAAACCCAGGCCAACAAGCTGCGGGCACG
GACCCGGGACGCCCTGGGCCCCAAGCACAAGGAGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT