POLR3E ORF clone pFN21AA1452

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AA1452

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Clone info.

Species

human

Clone Name

pFN21AA1452 Sequence (with vector) Restriction Map

Product ID

FHC00231

Flexi Clone PID

FXC00231

Original Clone PID

ORK00231

ORF Sequence

DNA sequence (2124 bp)
Protein sequence (708 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384602]

Gene

KIAA1452

Symbol

POLR3E

Alias : C37, RPC5, SIN

Description

polymerase (RNA) III (DNA directed) polypeptide E (80kD), transcript variant 1

Original Clone ID

fk08867

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2124 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 708 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10605.1 0 100.0 POLR3E CCDS73845.1 3.9e-219 100.0 POLR3E CCDS58434.1 3.6e-215 99.0 POLR3E CCDS58432.1 1.5e-210 97.0 POLR3E CCDS58433.1 2.9e-210 94.1 POLR3E The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006886 4 433 PF04801 DNA-directed RNA polymerase III subunit Rpc5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1452 Download

>KIAA1452  2124 bp
ATGGCCAATGAAGAGGATGACCCAGTTGTACAGGAGATCGATGTGTACTTGGCCAAGAGT
CTGGCGGAAAAGCTGTATCTATTTCAGTACCCTGTGCGTCCAGCCTCGATGACCTACGAT
GACATTCCGCACCTCTCAGCCAAGATCAAGCCCAAGCAGCAGAAGGTAGAGCTTGAGATG
GCCATCGACACCCTGAACCCCAACTATTGCCGCAGCAAAGGGGAGCAGATTGCGCTGAAC
GTGGACGGGGCCTGCGCCGACGAGACCAGCACGTATTCCTCGAAGCTGATGGACAAGCAG
ACCTTCTGCTCTTCCCAGACCACCAGTAACACATCCCGTTATGCCGCTGCACTCTACAGG
CAAGGTGAGCTCCACCTGACACCTTTACATGGCATCCTGCAGCTGCGGCCCAGCTTCTCC
TACCTGGATAAGGCTGACGCCAAGCACCGGGAGAGGGAGGCGGCCAACGAGGCAGGGGAC
TCTTCACAGGATGAGGCGGAAGACGATGTTAAGCAGATCACGGTGCGGTTCTCCCGGCCG
GAGTCAGAGCAGGCCCGCCAGCGCCGTGTGCAGTCCTATGAGTTCCTGCAGAAGAAGCAC
GCAGAGGAGCCCTGGGTCCACCTGCATTACTATGGCCTGAGGGACAGTCGCTCTGAGCAT
GAGCGTCAGTACCTGCTGTGCCCCGGCTCAAGCGGGGTGGAGAACACGGAGCTCGTCAAG
TCACCCAGTGAGTACCTGATGATGCTGATGCCACCCAGCCAGGAGGAGGAGAAAGACAAG
CCTGTGGCCCCCAGCAACGTCCTGTCGATGGCCCAGCTGCGCACGCTGCCCCTGGCCGAT
CAGATCAAGATCCTGATGAAGAATGTGAAGGTCATGCCTTTTGCCAACTTGATGAGCCTC
CTGGGCCCCTCCATCGATTCCGTGGCTGTTCTGCGGGGCATCCAGAAGGTGGCGATGTTG
GTCCAAGGGAACTGGGTGGTGAAGAGTGACATCCTATACCCCAAGGACTCGTCCAGCCCT
CACAGCGGCGTGCCTGCTGAGGTGCTCTGCAGGGGCCGAGACTTCGTTATGTGGAAGTTC
ACGCAGAGCCGCTGGGTGGTTAGGAAAGAGGTGGCAACCGTGACCAAACTCTGCGCCGAG
GATGTGAAGGACTTCCTGGAGCACATGGCCGTGGTGAGGATCAACAAAGGCTGGGAGTTC
ATTCTGCCTTATGATGGGGAGTTCATCAAGAAGCACCCGGATGTGGTCCAGCGGCAGCAC
ATGCTGTGGACGGGTATCCAGGCCAAACTGGAAAAAGTCTATAATCTTGTAAAGGAAACC
ATGCCAAAGAAGCCGGATGCACAATCAGGGCCTGCCGGGCTGGTCTGTGGGGACCAGCGG
ATCCAAGTAGCCAAAACCAAGGCCCAGCAGAACCACGCGTTGCTGGAGCGGGAGCTGCAG
CGGCGGAAGGAGCAGCTGCGGGTGCCTGCGGTCCCGCCCGGTGTGCGGATCAAGGAGGAG
CCCGTGAGCGAGGAGGGCGAGGAGGACGAGGAGCAGGAGGCGGAGGAGGAGCCCATGGAC
ACTTCCCCCAGCGGCCTCCACAGCAAGCTGGCCAACGGGCTGCCTCTCGGGCGGGCTGCG
GGCACAGACAGCTTCAACGGGCACCCGCCCCAGGGCTGCGCCAGCACCCCTGTGGCTCGG
GAACTGAAGGCCTTCGTGGAGGCCACCTTTCAGAGACAGTTTGTGCTCACGCTGAGCGAA
CTCAAGCGCCTCTTCAATCTGCACTTGGCCAGCCTGCCCCCCGGCCACACACTCTTCAGC
GGCATCTCGGACCGCATGCTACAGGACACGGTGCTGGCCGCCGGTTGCAAGCAGATACTG
GTGCCTTTTCCCCCCCAGACTGCTGCTTCCCCGGATGAGCAGAAGGTGTTTGCCCTCTGG
GAGTCTGGAGACATGAGTGATCAGCATCGACAGGTTTTGCTTGAAATTTTTTCCAAAAAT
TACCGGGTACGCCGAAACATGATCCAGTCTCGGTTGACTCAAGAGTGTGGAGAAGATCTC
AGTAAACAGGAGGTGGATAAAGTACTAAAGGACTGCTGTGTAAGCTATGGTGGCATGTGG
TACCTTAAAGGGACAGTACAGTCT

ORF Protein

Cloned ORF protein sequence for pFN21AA1452 Download

>KIAA1452  708 aa
MANEEDDPVVQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPHLSAKIKPKQQKVELEM
AIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTFCSSQTTSNTSRYAAALYR
QGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGDSSQDEAEDDVKQITVRFSRP
ESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGLRDSRSEHERQYLLCPGSSGVENTELVK
SPSEYLMMLMPPSQEEEKDKPVAPSNVLSMAQLRTLPLADQIKILMKNVKVMPFANLMSL
LGPSIDSVAVLRGIQKVAMLVQGNWVVKSDILYPKDSSSPHSGVPAEVLCRGRDFVMWKF
TQSRWVVRKEVATVTKLCAEDVKDFLEHMAVVRINKGWEFILPYDGEFIKKHPDVVQRQH
MLWTGIQAKLEKVYNLVKETMPKKPDAQSGPAGLVCGDQRIQVAKTKAQQNHALLERELQ
RRKEQLRVPAVPPGVRIKEEPVSEEGEEDEEQEAEEEPMDTSPSGLHSKLANGLPLGRAA
GTDSFNGHPPQGCASTPVARELKAFVEATFQRQFVLTLSELKRLFNLHLASLPPGHTLFS
GISDRMLQDTVLAAGCKQILVPFPPQTAASPDEQKVFALWESGDMSDQHRQVLLEIFSKN
YRVRRNMIQSRLTQECGEDLSKQEVDKVLKDCCVSYGGMWYLKGTVQS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1452 Download

>pFN21AA1452  6829 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAATGAAGAGGATGACCCAGTTGTACAGGA
GATCGATGTGTACTTGGCCAAGAGTCTGGCGGAAAAGCTGTATCTATTTCAGTACCCTGT
GCGTCCAGCCTCGATGACCTACGATGACATTCCGCACCTCTCAGCCAAGATCAAGCCCAA
GCAGCAGAAGGTAGAGCTTGAGATGGCCATCGACACCCTGAACCCCAACTATTGCCGCAG
CAAAGGGGAGCAGATTGCGCTGAACGTGGACGGGGCCTGCGCCGACGAGACCAGCACGTA
TTCCTCGAAGCTGATGGACAAGCAGACCTTCTGCTCTTCCCAGACCACCAGTAACACATC
CCGTTATGCCGCTGCACTCTACAGGCAAGGTGAGCTCCACCTGACACCTTTACATGGCAT
CCTGCAGCTGCGGCCCAGCTTCTCCTACCTGGATAAGGCTGACGCCAAGCACCGGGAGAG
GGAGGCGGCCAACGAGGCAGGGGACTCTTCACAGGATGAGGCGGAAGACGATGTTAAGCA
GATCACGGTGCGGTTCTCCCGGCCGGAGTCAGAGCAGGCCCGCCAGCGCCGTGTGCAGTC
CTATGAGTTCCTGCAGAAGAAGCACGCAGAGGAGCCCTGGGTCCACCTGCATTACTATGG
CCTGAGGGACAGTCGCTCTGAGCATGAGCGTCAGTACCTGCTGTGCCCCGGCTCAAGCGG
GGTGGAGAACACGGAGCTCGTCAAGTCACCCAGTGAGTACCTGATGATGCTGATGCCACC
CAGCCAGGAGGAGGAGAAAGACAAGCCTGTGGCCCCCAGCAACGTCCTGTCGATGGCCCA
GCTGCGCACGCTGCCCCTGGCCGATCAGATCAAGATCCTGATGAAGAATGTGAAGGTCAT
GCCTTTTGCCAACTTGATGAGCCTCCTGGGCCCCTCCATCGATTCCGTGGCTGTTCTGCG
GGGCATCCAGAAGGTGGCGATGTTGGTCCAAGGGAACTGGGTGGTGAAGAGTGACATCCT
ATACCCCAAGGACTCGTCCAGCCCTCACAGCGGCGTGCCTGCTGAGGTGCTCTGCAGGGG
CCGAGACTTCGTTATGTGGAAGTTCACGCAGAGCCGCTGGGTGGTTAGGAAAGAGGTGGC
AACCGTGACCAAACTCTGCGCCGAGGATGTGAAGGACTTCCTGGAGCACATGGCCGTGGT
GAGGATCAACAAAGGCTGGGAGTTCATTCTGCCTTATGATGGGGAGTTCATCAAGAAGCA
CCCGGATGTGGTCCAGCGGCAGCACATGCTGTGGACGGGTATCCAGGCCAAACTGGAAAA
AGTCTATAATCTTGTAAAGGAAACCATGCCAAAGAAGCCGGATGCACAATCAGGGCCTGC
CGGGCTGGTCTGTGGGGACCAGCGGATCCAAGTAGCCAAAACCAAGGCCCAGCAGAACCA
CGCGTTGCTGGAGCGGGAGCTGCAGCGGCGGAAGGAGCAGCTGCGGGTGCCTGCGGTCCC
GCCCGGTGTGCGGATCAAGGAGGAGCCCGTGAGCGAGGAGGGCGAGGAGGACGAGGAGCA
GGAGGCGGAGGAGGAGCCCATGGACACTTCCCCCAGCGGCCTCCACAGCAAGCTGGCCAA
CGGGCTGCCTCTCGGGCGGGCTGCGGGCACAGACAGCTTCAACGGGCACCCGCCCCAGGG
CTGCGCCAGCACCCCTGTGGCTCGGGAACTGAAGGCCTTCGTGGAGGCCACCTTTCAGAG
ACAGTTTGTGCTCACGCTGAGCGAACTCAAGCGCCTCTTCAATCTGCACTTGGCCAGCCT
GCCCCCCGGCCACACACTCTTCAGCGGCATCTCGGACCGCATGCTACAGGACACGGTGCT
GGCCGCCGGTTGCAAGCAGATACTGGTGCCTTTTCCCCCCCAGACTGCTGCTTCCCCGGA
TGAGCAGAAGGTGTTTGCCCTCTGGGAGTCTGGAGACATGAGTGATCAGCATCGACAGGT
TTTGCTTGAAATTTTTTCCAAAAATTACCGGGTACGCCGAAACATGATCCAGTCTCGGTT
GACTCAAGAGTGTGGAGAAGATCTCAGTAAACAGGAGGTGGATAAAGTACTAAAGGACTG
CTGTGTAAGCTATGGTGGCATGTGGTACCTTAAAGGGACAGTACAGTCTGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT