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SpecieshumanProduct IDFHC00183Cloning SiteSgfI-PmeISymbolDSCAML1
Alias : DSCAM2DescriptionDown syndrome cell adhesion molecule like 1
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 6159 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 2053 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS8384.1 0 100.0 DSCAML1 CCDS42929.1 3.8e-194 59.8 DSCAM CCDS43041.1 3.8e-14 24.5 CNTN4 CCDS30956.1 3.3e-13 24.0 HMCN1 CCDS33790.1 1.1e-12 24.8 CNTN3
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition HMMPfam IPR013098 229 309 PF07679 Immunoglobulin I-set IPR013098 317 396 PF07679 Immunoglobulin I-set IPR013098 408 502 PF07679 Immunoglobulin I-set IPR013098 510 578 PF07679 Immunoglobulin I-set IPR013098 608 686 PF07679 Immunoglobulin I-set IPR013098 690 782 PF07679 Immunoglobulin I-set IPR013098 791 884 PF07679 Immunoglobulin I-set IPR003961 889 974 PF00041 Fibronectin IPR003961 989 1078 PF00041 Fibronectin IPR003961 1093 1179 PF00041 Fibronectin IPR003961 1193 1277 PF00041 Fibronectin IPR013098 1305 1380 PF07679 Immunoglobulin I-set IPR003961 1388 1467 PF00041 Fibronectin HMMSmart IPR003599 32 122 SM00409 Immunoglobulin subtype IPR003598 38 110 SM00408 Immunoglobulin subtype 2 IPR003599 131 219 SM00409 Immunoglobulin subtype IPR003599 232 312 SM00409 Immunoglobulin subtype IPR003598 238 301 SM00408 Immunoglobulin subtype 2 IPR003596 242 296 SM00406 Immunoglobulin V-set IPR003599 321 403 SM00409 Immunoglobulin subtype IPR003598 327 393 SM00408 Immunoglobulin subtype 2 IPR003599 414 503 SM00409 Immunoglobulin subtype IPR003598 420 492 SM00408 Immunoglobulin subtype 2 IPR003599 511 594 SM00409 Immunoglobulin subtype IPR003598 517 582 SM00408 Immunoglobulin subtype 2 IPR003599 602 687 SM00409 Immunoglobulin subtype IPR003598 608 676 SM00408 Immunoglobulin subtype 2 IPR003599 696 786 SM00409 Immunoglobulin subtype IPR003598 702 774 SM00408 Immunoglobulin subtype 2 IPR003599 795 885 SM00409 Immunoglobulin subtype IPR003598 801 874 SM00408 Immunoglobulin subtype 2 IPR003961 887 971 SM00060 Fibronectin IPR003961 987 1075 SM00060 Fibronectin IPR003961 1090 1176 SM00060 Fibronectin IPR003961 1191 1274 SM00060 Fibronectin IPR003599 1299 1381 SM00409 Immunoglobulin subtype IPR003598 1305 1370 SM00408 Immunoglobulin subtype 2 IPR003596 1306 1365 SM00406 Immunoglobulin V-set IPR003961 1384 1464 SM00060 Fibronectin IPR003961 1479 1560 SM00060 Fibronectin ProfileScan IPR007110 18 107 PS50835 Immunoglobulin-like IPR007110 115 217 PS50835 Immunoglobulin-like IPR007110 226 306 PS50835 Immunoglobulin-like IPR007110 314 402 PS50835 Immunoglobulin-like IPR007110 408 501 PS50835 Immunoglobulin-like IPR007110 506 586 PS50835 Immunoglobulin-like IPR007110 596 685 PS50835 Immunoglobulin-like IPR007110 690 784 PS50835 Immunoglobulin-like IPR007110 788 885 PS50835 Immunoglobulin-like IPR003961 887 980 PS50853 Fibronectin IPR003961 986 1085 PS50853 Fibronectin IPR003961 1090 1186 PS50853 Fibronectin IPR003961 1191 1283 PS50853 Fibronectin IPR007110 1278 1377 PS50835 Immunoglobulin-like IPR003961 1380 1474 PS50853 Fibronectin IPR003961 1479 1569 PS50853 Fibronectin
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 32 LQQVTFSSSVGVVVPCPAAGSP 53 SECONDARY 22 2 1592 LFTIGCPVILATLGVALLFIV 1612 PRIMARY 21
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065744 2113 611782 100.0 100.0 XP_011541220 1977 611782 99.3 95.5 XP_011541219 2065 611782 99.4 100.0 NP_001380 2012 602523 59.8 100.0 NP_001258463 1994 602523 59.4 100.0 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AA1132 Download>KIAA1132 6159 bp ATGTGGCTGGTAACTTTCCTCCTGCTCCTGGACTCTTTACACAAAGCCCGCCCTGAAGAT GTTGGCACCAGCCTCTACTTTGTAAATGACTCCTTGCAGCAGGTGACCTTTTCCAGCTCC GTGGGGGTGGTGGTGCCCTGCCCGGCCGCGGGCTCCCCCAGCGCGGCCCTTCGATGGTAC CTGGCCACAGGGGACGACATCTACGACGTGCCGCACATCCGGCACGTCCACGCCAACGGG ACGCTGCAGCTCTACCCCTTCTCCCCCTCCGCCTTCAATAGCTTTATCCACGACAATGAC TACTTCTGCACCGCGGAGAACGCTGCCGGCAAGATCCGGAGCCCCAACATCCGCGTCAAA GCAGTTTTCAGGGAACCCTACACCGTCCGGGTGGAGGATCAAAGGTCAATGCGTGGCAAC GTGGCCGTCTTCAAGTGCCTCATCCCCTCTTCAGTGCAGGAATATGTTAGCGTTGTATCT TGGGAGAAAGACACAGTCTCCATCATCCCAGAAAACAGGTTTTTTATTACCTACCACGGC GGGCTGTACATCTCTGACGTACAGAAGGAGGACGCCCTCTCCACCTATCGCTGCATCACC AAGCACAAGTATAGCGGGGAGACCCGGCAGAGCAATGGGGCACGCCTCTCTGTGACAGAC CCTGCTGAGTCGATCCCCACCATCCTGGATGGCTTCCACTCCCAGGAAGTGTGGGCCGGC CACACCGTGGAGCTGCCCTGCACCGCCTCGGGCTACCCTATCCCCGCCATCCGCTGGCTC AAGGATGGCCGGCCCCTCCCGGCTGACAGCCGCTGGACCAAGCGCATCACAGGGCTGACC ATCAGCGACTTGCGGACCGAGGACAGCGGCACCTACATTTGTGAGGTCACCAACACCTTC GGTTCGGCAGAGGCCACAGGCATCCTCATGGTCATTGATCCCCTTCATGTGACCCTGACA CCAAAGAAGCTGAAGACCGGCATTGGCAGCACGGTCATCCTCTCCTGTGCCCTGACGGGC TCCCCAGAGTTCACCATCCGCTGGTATCGCAACACGGAGCTGGTGCTGCCTGACGAGGCC ATCTCCATCCGTGGGCTCAGCAACGAGACGCTGCTCATCACCTCGGCCCAGAAGAGCCAT TCCGGGGCCTACCAGTGCTTCGCTACCCGCAAGGCCCAGACCGCCCAGGACTTTGCCATC ATTGCACTTGAGGATGGCACGCCCCGCATCGTCTCGTCCTTCAGCGAGAAGGTGGTCAAC CCCGGGGAGCAGTTCTCACTGATGTGTGCGGCCAAGGGCGCCCCGCCCCCCACGGTCACC TGGGCCCTCGACGATGAGCCCATCGTGCGGGATGGCAGCCACCGCACCAACCAGTACACC ATGTCGGACGGCACCACCATCAGCCACATGAACGTCACAGGCCCCCAGATCCGCGACGGG GGCGTGTACCGGTGCACAGCGCGGAACTTGGTGGGCAGTGCTGAATATCAGGCGCGAATA AACGTAAGAGGCCCACCCAGCATCCGGGCTATGCGGAACATCACAGCAGTCGCCGGGCGG GACACCCTTATCAACTGCAGGGTCATCGGCTATCCCTACTACTCCATCAAGTGGTACAAG GATGCCCTGCTGCTGCCAGACAACCACCGCCAGGTGGTGTTTGAGAATGGGACCCTCAAG CTGACTGACGTGCAGAAGGGCATGGATGAGGGGGAGTACCTGTGCAGTGTCCTCATCCAG CCCCAGCTCTCCATCAGCCAGAGCGTTCACGTAGCCGTCAAAGTGCCCCCTCTGATCCAG CCCTTCGAATTCCCACCCGCCTCCATCGGCCAGCTGCTCTACATTCCCTGTGTGGTGTCC TCGGGGGACATGCCCATCCGTATCACCTGGAGGAAGGACGGACAGGTGATCATCTCAGGC TCGGGCGTGACCATCGAGAGCAAGGAATTCATGAGCTCCCTGCAGATCTCTAGCGTCTCC CTCAAGCACAACGGCAACTATACATGCATCGCCAGCAACGCAGCCGCCACCGTGAGCCGG GAGCGCCAGCTCATCGTGCGTGTGCCCCCTCGATTTGTGGTGCAACCCAACAACCAGGAT GGCATCTACGGCAAAGCTGGTGTGCTCAACTGCTCGGTGGACGGCTACCCCCCACCCAAG GTCATGTGGAAGCATGCCAAGGGGAGCGGGAACCCCCAGCAGTACCACCCTGTGCCCCTC ACTGGCCGCATCCAGATCCTGCCCAACAGCTCGCTGCTGATCCGCCACGTCCTAGAAGAG GACATCGGCTACTACCTCTGCCAGGCCAGCAACGGCGTAGGCACCGACATCAGCAAGTCC ATGTTCCTCACAGTCAAGATCCCGGCCATGATCACTTCCCACCCCAACACCACCATCGCC ATCAAGGGCCATGCGAAGGAGCTAAACTGCACGGCACGGGGTGAGCGGCCCATCATCATC CGCTGGGAGAAGGGGGACACAGTCATCGACCCTGACCGCGTCATGCGGTATGCCATCGCC ACCAAGGACAACGGCGACGAGGTCGTCTCCACACTGAAGCTCAAGCCCGCTGACCGTGGG GACTCTGTGTTCTTCAGCTGCCATGCCATCAACTCGTATGGGGAGGACCGGGGCTTGATC CAACTCACTGTGCAAGAGCCCCCCGACCCCCCAGAGCTGGAGATCCGGGAGGTGAAGGCC CGGAGCATGAACCTGCGCTGGACCCAGCGATTCGACGGGAACAGCATCATCACGGGCTTC GACATTGAATACAAGAACAAATCAGATTCCTGGGACTTCAAGCAGTCCACACGCAACATC TCCCCCACCATCAACCAGGCCAACATTGTGGACTTGCACCCGGCATCTGTGTACAGCATC CGCATGTACTCTTTCAACAAGATTGGCCGCAGTGAACCAAGCAAGGAGCTCACCATCAGC ACTGAGGAGGCCGCTCCCGATGGGCCCCCCATGGATGTTACCTTGCAGCCAGTGACCTCA CAGAGCATCCAGGTGACCTGGAAGGCACCCAAGAAGGAGCTGCAGAACGGTGTCATCCGG GGCTACCAGATTGGCTACAGAGAGAACAGCCCCGGCAGCAACGGGCAGTACAGCATCGTG GAGATGAAGGCCACGGGGGACAGCGAGGTCTACACCCTGGACAACCTCAAGAAGTTCGCC CAGTATGGGGTGGTGGTCCAAGCCTTCAATCGGGCTGGCACGGGGCCCTCTTCCAGCGAG ATCAATGCCACCACTCTGGAGGATGTGCCCAGCCAGCCCCCTGAGAACGTCCGGGCCCTG TCCATCACTTCTGACGTGGCCGTCATCTCCTGGTCAGAGCCCCCGCGCAGCACCCTCAAT GGCGTCCTCAAAGGCTATCGGGTCATCTTCTGGTCCCTCTATGTTGATGGGGAGTGGGGC GAGATGCAGAACATCACCACCACGCGGGAGCGGGTGGAGCTGCGGGGCATGGAGAAGTTC ACCAACTACAGCGTCCAGGTGCTGGCCTACACCCAGGCTGGGGACGGCGTACGCAGCAGT GTGCTCTACATCCAGACCAAGGAGGACGTTCCAGGTCCCCCTGCTGGCATCAAAGCTGTC CCTTCATCAGCTAGCAGTGTGGTTGTGTCTTGGCTCCCCCCTACCAAGCCCAACGGGGTG ATCCGCAAGTACACCATCTTCTGTTCCAGCCCCGGGTCTGGCCAGCCGGCTCCCAGCGAG TACGAGACGAGTCCAGAGCAGCTCTTCTACCGGATCGCCCACCTAAACCGCGGTCAGCAG TATCTGCTGTGGGTGGCCGCCGTCACCTCTGCCGGCCGGGGCAACAGCAGCGAGAAGGTG ACCATCGAGCCTGCTGGCAAGGCCCCAGCAAAGATCATCTCCTTTGGGGGCACCGTGACA ACACCTTGGATGAAAGATGTTCGGCTGCCTTGCAATTCAGTGGGAGATCCAGCCCCTGCT GTGAAGTGGACCAAGGACAGTGAAGACTCGGCCATTCCAGTGTCCATGGATGGGCACCGG CTCATCCACACCAATGGCACACTGCTGCTGCGTGCAGTGAAGGCTGAGGACTCTGGCTAC TACACGTGCACGGCCACCAACACTGGTGGCTTTGACACCATCATCGTCAACCTTCTGGTG CAAGTTCCCCCGGACCAGCCCCGCCTCACTGTCTCCAAAACCTCAGCTTCGTCCATCACC CTGACCTGGATTCCAGGTGACAATGGGGGCAGCTCCATCCGAGGCTTCGTGCTACAGTAC TCGGTGGACAACAGCGAGGAGTGGAAGGATGTGTTCATCAGCTCCAGCGAGCGCTCCTTC AAGCTGGACAGCCTCAAGTGTGGCACGTGGTACAAGGTGAAGCTGGCAGCCAAGAACAGC GTGGGCTCTGGGCGCATCAGCGAGATCATCGAGGCCAAGACCCACGGGCGGGAGCCCTCC TTCAGCAAAGACCAACACCTCTTCACCCACATCAACTCCACGCATGCTCGGCTTAACCTG CAGGGCTGGAACAATGGGGGCTGCCCTATCACAGCCATCGTTCTGGAGTACCGGCCCAAG GGGACCTGGGCCTGGCAGGGCCTCCGGGCCAACAGCTCCGGGGAGGTGTTTCTGACGGAA CTGCGAGAGGCCACGTGGTACGAGCTGCGCATGAGGGCTTGCAACAGTGCGGGCTGCGGC AATGAAACAGCCCAGTTCGCCACCCTGGACTACGATGGCAGCACCATTCCACCCATCAAG TCTGCTCAAGGTGAAGGGGATGATGTGAAGAAGCTGTTCACCATCGGCTGCCCTGTCATC CTGGCCACACTGGGGGTGGCACTGCTCTTCATCGTACGCAAGAAGAGGAAGGAGAAACGG CTGAAGCGACTCCGAGATGCAAAGAGTTTGGCAGAAATGTTGATAAGCAAGAACAATAGA AGCTTTGACACCCCTGTGAAAGGGCCACCCCAGGGCCCACGGCTACACATTGACATCCCC AGGGTCCAGCTGCTCATCGAGGACAAAGAAGGCATCAAGCAACTGGGAGATGACAAGGCC ACCATCCCTGTGACAGATGCTGAGTTCAGCCAAGCTGTCAACCCACAGAGCTTCTGTACT GGCGTCTCCTTGCACCACCCAACCCTCATCCAGAGCACAGGACCCCTCATCGACATGTCT GACATCCGGCCAGGAACCAATCCAGTGTCCAGGAAGAATGTGAAGTCAGCCCACAGCACC CGGAACCGGTACTCAAGCCAGTGGACCCTGACCAAGTGCCAGGCCTCCACACCTGCCCGC ACCCTCACCTCCGACTGGCGCACCGTGGGCTCCCAGCATGGTGTCACGGTCACTGAGAGT GACAGCTACAGTGCCAGCCTGTCCCAGGACACAGACAAAGGAAGGAACAGCATGGTGTCC ACTGAGAGTGCCTCTTCCACCTACGAGGAGCTGGCCCGGGCCTATGAGCATGCCAAGCTG GAGGAGCAGCTGCAGCACGCCAAGTTTGAGATCACCGAGTGCTTCATCTCTGACAGTTCC TCTGACCAGATGACCACAGGCACCAACGAGAACGCCGACAGCATGACATCCATGAGCACA CCCTCAGAGCCTGGCATCTGCCGCTTTACCGCCTCACCACCCAAGCCCCAGGATGCGGAC CGGGGCAAAAACGTGGCTGTGCCCATCCCTCACCGGGCCAACAAGAGTGACTACTGCAAC CTGCCCCTGTATGCCAAGTCAGAGGCCTTCTTTCGAAAGGCAGATGGACGTGAGCCCTGC CCCGTGGTCCCACCCCGTGAGGCCTCCATCCGGAACCTGGCTCGAACCTACCACACCCAG GCTCGCCACCTGACCCTGGACCCTGCCAGCAAGTCCTTGGGCCTTCCCCACCCAGGGGCC CCCGCTGCCGCCTCCACAGCCACCTTACCTCAGAGGACTCTGGCCATGCCAGCCCCCCCA GCCGGCACAGCCCCCCCAGCCCCCGGCCCCACCCCTGCTGAGCCACCCACCGCCCCCAGC GCTGCCCCTCCGGCCCCCAGCACCGAGCCTCCACGAGCCGGGGGCCCACACACCAAAATG GGGGGCTCCAGGGACTCGCTTCTCGAGATGAGCACATCGGGGGTAGGGAGGTCTCAGAAG CAGGGGGCCGGGGCCTACTCCAAATCCTACACCCTGGTG
Cloned ORF protein sequence for pFN21AA1132 Download>KIAA1132 2053 aa MWLVTFLLLLDSLHKARPEDVGTSLYFVNDSLQQVTFSSSVGVVVPCPAAGSPSAALRWY LATGDDIYDVPHIRHVHANGTLQLYPFSPSAFNSFIHDNDYFCTAENAAGKIRSPNIRVK AVFREPYTVRVEDQRSMRGNVAVFKCLIPSSVQEYVSVVSWEKDTVSIIPENRFFITYHG GLYISDVQKEDALSTYRCITKHKYSGETRQSNGARLSVTDPAESIPTILDGFHSQEVWAG HTVELPCTASGYPIPAIRWLKDGRPLPADSRWTKRITGLTISDLRTEDSGTYICEVTNTF GSAEATGILMVIDPLHVTLTPKKLKTGIGSTVILSCALTGSPEFTIRWYRNTELVLPDEA ISIRGLSNETLLITSAQKSHSGAYQCFATRKAQTAQDFAIIALEDGTPRIVSSFSEKVVN PGEQFSLMCAAKGAPPPTVTWALDDEPIVRDGSHRTNQYTMSDGTTISHMNVTGPQIRDG GVYRCTARNLVGSAEYQARINVRGPPSIRAMRNITAVAGRDTLINCRVIGYPYYSIKWYK DALLLPDNHRQVVFENGTLKLTDVQKGMDEGEYLCSVLIQPQLSISQSVHVAVKVPPLIQ PFEFPPASIGQLLYIPCVVSSGDMPIRITWRKDGQVIISGSGVTIESKEFMSSLQISSVS LKHNGNYTCIASNAAATVSRERQLIVRVPPRFVVQPNNQDGIYGKAGVLNCSVDGYPPPK VMWKHAKGSGNPQQYHPVPLTGRIQILPNSSLLIRHVLEEDIGYYLCQASNGVGTDISKS MFLTVKIPAMITSHPNTTIAIKGHAKELNCTARGERPIIIRWEKGDTVIDPDRVMRYAIA TKDNGDEVVSTLKLKPADRGDSVFFSCHAINSYGEDRGLIQLTVQEPPDPPELEIREVKA RSMNLRWTQRFDGNSIITGFDIEYKNKSDSWDFKQSTRNISPTINQANIVDLHPASVYSI RMYSFNKIGRSEPSKELTISTEEAAPDGPPMDVTLQPVTSQSIQVTWKAPKKELQNGVIR GYQIGYRENSPGSNGQYSIVEMKATGDSEVYTLDNLKKFAQYGVVVQAFNRAGTGPSSSE INATTLEDVPSQPPENVRALSITSDVAVISWSEPPRSTLNGVLKGYRVIFWSLYVDGEWG EMQNITTTRERVELRGMEKFTNYSVQVLAYTQAGDGVRSSVLYIQTKEDVPGPPAGIKAV PSSASSVVVSWLPPTKPNGVIRKYTIFCSSPGSGQPAPSEYETSPEQLFYRIAHLNRGQQ YLLWVAAVTSAGRGNSSEKVTIEPAGKAPAKIISFGGTVTTPWMKDVRLPCNSVGDPAPA VKWTKDSEDSAIPVSMDGHRLIHTNGTLLLRAVKAEDSGYYTCTATNTGGFDTIIVNLLV QVPPDQPRLTVSKTSASSITLTWIPGDNGGSSIRGFVLQYSVDNSEEWKDVFISSSERSF KLDSLKCGTWYKVKLAAKNSVGSGRISEIIEAKTHGREPSFSKDQHLFTHINSTHARLNL QGWNNGGCPITAIVLEYRPKGTWAWQGLRANSSGEVFLTELREATWYELRMRACNSAGCG NETAQFATLDYDGSTIPPIKSAQGEGDDVKKLFTIGCPVILATLGVALLFIVRKKRKEKR LKRLRDAKSLAEMLISKNNRSFDTPVKGPPQGPRLHIDIPRVQLLIEDKEGIKQLGDDKA TIPVTDAEFSQAVNPQSFCTGVSLHHPTLIQSTGPLIDMSDIRPGTNPVSRKNVKSAHST RNRYSSQWTLTKCQASTPARTLTSDWRTVGSQHGVTVTESDSYSASLSQDTDKGRNSMVS TESASSTYEELARAYEHAKLEEQLQHAKFEITECFISDSSSDQMTTGTNENADSMTSMST PSEPGICRFTASPPKPQDADRGKNVAVPIPHRANKSDYCNLPLYAKSEAFFRKADGREPC PVVPPREASIRNLARTYHTQARHLTLDPASKSLGLPHPGAPAAASTATLPQRTLAMPAPP AGTAPPAPGPTPAEPPTAPSAAPPAPSTEPPRAGGPHTKMGGSRDSLLEMSTSGVGRSQK QGAGAYSKSYTLV
Nucleotide Sequence (with vector) for pFN21AA1132 Download>pFN21AA1132 10864 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGTGGCTGGTAACTTTCCTCCTGCTCCTGGACTC TTTACACAAAGCCCGCCCTGAAGATGTTGGCACCAGCCTCTACTTTGTAAATGACTCCTT GCAGCAGGTGACCTTTTCCAGCTCCGTGGGGGTGGTGGTGCCCTGCCCGGCCGCGGGCTC CCCCAGCGCGGCCCTTCGATGGTACCTGGCCACAGGGGACGACATCTACGACGTGCCGCA CATCCGGCACGTCCACGCCAACGGGACGCTGCAGCTCTACCCCTTCTCCCCCTCCGCCTT CAATAGCTTTATCCACGACAATGACTACTTCTGCACCGCGGAGAACGCTGCCGGCAAGAT CCGGAGCCCCAACATCCGCGTCAAAGCAGTTTTCAGGGAACCCTACACCGTCCGGGTGGA GGATCAAAGGTCAATGCGTGGCAACGTGGCCGTCTTCAAGTGCCTCATCCCCTCTTCAGT GCAGGAATATGTTAGCGTTGTATCTTGGGAGAAAGACACAGTCTCCATCATCCCAGAAAA CAGGTTTTTTATTACCTACCACGGCGGGCTGTACATCTCTGACGTACAGAAGGAGGACGC CCTCTCCACCTATCGCTGCATCACCAAGCACAAGTATAGCGGGGAGACCCGGCAGAGCAA TGGGGCACGCCTCTCTGTGACAGACCCTGCTGAGTCGATCCCCACCATCCTGGATGGCTT CCACTCCCAGGAAGTGTGGGCCGGCCACACCGTGGAGCTGCCCTGCACCGCCTCGGGCTA CCCTATCCCCGCCATCCGCTGGCTCAAGGATGGCCGGCCCCTCCCGGCTGACAGCCGCTG GACCAAGCGCATCACAGGGCTGACCATCAGCGACTTGCGGACCGAGGACAGCGGCACCTA CATTTGTGAGGTCACCAACACCTTCGGTTCGGCAGAGGCCACAGGCATCCTCATGGTCAT TGATCCCCTTCATGTGACCCTGACACCAAAGAAGCTGAAGACCGGCATTGGCAGCACGGT CATCCTCTCCTGTGCCCTGACGGGCTCCCCAGAGTTCACCATCCGCTGGTATCGCAACAC GGAGCTGGTGCTGCCTGACGAGGCCATCTCCATCCGTGGGCTCAGCAACGAGACGCTGCT CATCACCTCGGCCCAGAAGAGCCATTCCGGGGCCTACCAGTGCTTCGCTACCCGCAAGGC CCAGACCGCCCAGGACTTTGCCATCATTGCACTTGAGGATGGCACGCCCCGCATCGTCTC GTCCTTCAGCGAGAAGGTGGTCAACCCCGGGGAGCAGTTCTCACTGATGTGTGCGGCCAA GGGCGCCCCGCCCCCCACGGTCACCTGGGCCCTCGACGATGAGCCCATCGTGCGGGATGG CAGCCACCGCACCAACCAGTACACCATGTCGGACGGCACCACCATCAGCCACATGAACGT CACAGGCCCCCAGATCCGCGACGGGGGCGTGTACCGGTGCACAGCGCGGAACTTGGTGGG CAGTGCTGAATATCAGGCGCGAATAAACGTAAGAGGCCCACCCAGCATCCGGGCTATGCG GAACATCACAGCAGTCGCCGGGCGGGACACCCTTATCAACTGCAGGGTCATCGGCTATCC CTACTACTCCATCAAGTGGTACAAGGATGCCCTGCTGCTGCCAGACAACCACCGCCAGGT GGTGTTTGAGAATGGGACCCTCAAGCTGACTGACGTGCAGAAGGGCATGGATGAGGGGGA GTACCTGTGCAGTGTCCTCATCCAGCCCCAGCTCTCCATCAGCCAGAGCGTTCACGTAGC CGTCAAAGTGCCCCCTCTGATCCAGCCCTTCGAATTCCCACCCGCCTCCATCGGCCAGCT GCTCTACATTCCCTGTGTGGTGTCCTCGGGGGACATGCCCATCCGTATCACCTGGAGGAA GGACGGACAGGTGATCATCTCAGGCTCGGGCGTGACCATCGAGAGCAAGGAATTCATGAG CTCCCTGCAGATCTCTAGCGTCTCCCTCAAGCACAACGGCAACTATACATGCATCGCCAG CAACGCAGCCGCCACCGTGAGCCGGGAGCGCCAGCTCATCGTGCGTGTGCCCCCTCGATT TGTGGTGCAACCCAACAACCAGGATGGCATCTACGGCAAAGCTGGTGTGCTCAACTGCTC GGTGGACGGCTACCCCCCACCCAAGGTCATGTGGAAGCATGCCAAGGGGAGCGGGAACCC CCAGCAGTACCACCCTGTGCCCCTCACTGGCCGCATCCAGATCCTGCCCAACAGCTCGCT GCTGATCCGCCACGTCCTAGAAGAGGACATCGGCTACTACCTCTGCCAGGCCAGCAACGG CGTAGGCACCGACATCAGCAAGTCCATGTTCCTCACAGTCAAGATCCCGGCCATGATCAC TTCCCACCCCAACACCACCATCGCCATCAAGGGCCATGCGAAGGAGCTAAACTGCACGGC ACGGGGTGAGCGGCCCATCATCATCCGCTGGGAGAAGGGGGACACAGTCATCGACCCTGA CCGCGTCATGCGGTATGCCATCGCCACCAAGGACAACGGCGACGAGGTCGTCTCCACACT GAAGCTCAAGCCCGCTGACCGTGGGGACTCTGTGTTCTTCAGCTGCCATGCCATCAACTC GTATGGGGAGGACCGGGGCTTGATCCAACTCACTGTGCAAGAGCCCCCCGACCCCCCAGA GCTGGAGATCCGGGAGGTGAAGGCCCGGAGCATGAACCTGCGCTGGACCCAGCGATTCGA CGGGAACAGCATCATCACGGGCTTCGACATTGAATACAAGAACAAATCAGATTCCTGGGA CTTCAAGCAGTCCACACGCAACATCTCCCCCACCATCAACCAGGCCAACATTGTGGACTT GCACCCGGCATCTGTGTACAGCATCCGCATGTACTCTTTCAACAAGATTGGCCGCAGTGA ACCAAGCAAGGAGCTCACCATCAGCACTGAGGAGGCCGCTCCCGATGGGCCCCCCATGGA TGTTACCTTGCAGCCAGTGACCTCACAGAGCATCCAGGTGACCTGGAAGGCACCCAAGAA GGAGCTGCAGAACGGTGTCATCCGGGGCTACCAGATTGGCTACAGAGAGAACAGCCCCGG CAGCAACGGGCAGTACAGCATCGTGGAGATGAAGGCCACGGGGGACAGCGAGGTCTACAC CCTGGACAACCTCAAGAAGTTCGCCCAGTATGGGGTGGTGGTCCAAGCCTTCAATCGGGC TGGCACGGGGCCCTCTTCCAGCGAGATCAATGCCACCACTCTGGAGGATGTGCCCAGCCA GCCCCCTGAGAACGTCCGGGCCCTGTCCATCACTTCTGACGTGGCCGTCATCTCCTGGTC AGAGCCCCCGCGCAGCACCCTCAATGGCGTCCTCAAAGGCTATCGGGTCATCTTCTGGTC CCTCTATGTTGATGGGGAGTGGGGCGAGATGCAGAACATCACCACCACGCGGGAGCGGGT GGAGCTGCGGGGCATGGAGAAGTTCACCAACTACAGCGTCCAGGTGCTGGCCTACACCCA GGCTGGGGACGGCGTACGCAGCAGTGTGCTCTACATCCAGACCAAGGAGGACGTTCCAGG TCCCCCTGCTGGCATCAAAGCTGTCCCTTCATCAGCTAGCAGTGTGGTTGTGTCTTGGCT CCCCCCTACCAAGCCCAACGGGGTGATCCGCAAGTACACCATCTTCTGTTCCAGCCCCGG GTCTGGCCAGCCGGCTCCCAGCGAGTACGAGACGAGTCCAGAGCAGCTCTTCTACCGGAT CGCCCACCTAAACCGCGGTCAGCAGTATCTGCTGTGGGTGGCCGCCGTCACCTCTGCCGG CCGGGGCAACAGCAGCGAGAAGGTGACCATCGAGCCTGCTGGCAAGGCCCCAGCAAAGAT CATCTCCTTTGGGGGCACCGTGACAACACCTTGGATGAAAGATGTTCGGCTGCCTTGCAA TTCAGTGGGAGATCCAGCCCCTGCTGTGAAGTGGACCAAGGACAGTGAAGACTCGGCCAT TCCAGTGTCCATGGATGGGCACCGGCTCATCCACACCAATGGCACACTGCTGCTGCGTGC AGTGAAGGCTGAGGACTCTGGCTACTACACGTGCACGGCCACCAACACTGGTGGCTTTGA CACCATCATCGTCAACCTTCTGGTGCAAGTTCCCCCGGACCAGCCCCGCCTCACTGTCTC CAAAACCTCAGCTTCGTCCATCACCCTGACCTGGATTCCAGGTGACAATGGGGGCAGCTC CATCCGAGGCTTCGTGCTACAGTACTCGGTGGACAACAGCGAGGAGTGGAAGGATGTGTT CATCAGCTCCAGCGAGCGCTCCTTCAAGCTGGACAGCCTCAAGTGTGGCACGTGGTACAA GGTGAAGCTGGCAGCCAAGAACAGCGTGGGCTCTGGGCGCATCAGCGAGATCATCGAGGC CAAGACCCACGGGCGGGAGCCCTCCTTCAGCAAAGACCAACACCTCTTCACCCACATCAA CTCCACGCATGCTCGGCTTAACCTGCAGGGCTGGAACAATGGGGGCTGCCCTATCACAGC CATCGTTCTGGAGTACCGGCCCAAGGGGACCTGGGCCTGGCAGGGCCTCCGGGCCAACAG CTCCGGGGAGGTGTTTCTGACGGAACTGCGAGAGGCCACGTGGTACGAGCTGCGCATGAG GGCTTGCAACAGTGCGGGCTGCGGCAATGAAACAGCCCAGTTCGCCACCCTGGACTACGA TGGCAGCACCATTCCACCCATCAAGTCTGCTCAAGGTGAAGGGGATGATGTGAAGAAGCT GTTCACCATCGGCTGCCCTGTCATCCTGGCCACACTGGGGGTGGCACTGCTCTTCATCGT ACGCAAGAAGAGGAAGGAGAAACGGCTGAAGCGACTCCGAGATGCAAAGAGTTTGGCAGA AATGTTGATAAGCAAGAACAATAGAAGCTTTGACACCCCTGTGAAAGGGCCACCCCAGGG CCCACGGCTACACATTGACATCCCCAGGGTCCAGCTGCTCATCGAGGACAAAGAAGGCAT CAAGCAACTGGGAGATGACAAGGCCACCATCCCTGTGACAGATGCTGAGTTCAGCCAAGC TGTCAACCCACAGAGCTTCTGTACTGGCGTCTCCTTGCACCACCCAACCCTCATCCAGAG CACAGGACCCCTCATCGACATGTCTGACATCCGGCCAGGAACCAATCCAGTGTCCAGGAA GAATGTGAAGTCAGCCCACAGCACCCGGAACCGGTACTCAAGCCAGTGGACCCTGACCAA GTGCCAGGCCTCCACACCTGCCCGCACCCTCACCTCCGACTGGCGCACCGTGGGCTCCCA GCATGGTGTCACGGTCACTGAGAGTGACAGCTACAGTGCCAGCCTGTCCCAGGACACAGA CAAAGGAAGGAACAGCATGGTGTCCACTGAGAGTGCCTCTTCCACCTACGAGGAGCTGGC CCGGGCCTATGAGCATGCCAAGCTGGAGGAGCAGCTGCAGCACGCCAAGTTTGAGATCAC CGAGTGCTTCATCTCTGACAGTTCCTCTGACCAGATGACCACAGGCACCAACGAGAACGC CGACAGCATGACATCCATGAGCACACCCTCAGAGCCTGGCATCTGCCGCTTTACCGCCTC ACCACCCAAGCCCCAGGATGCGGACCGGGGCAAAAACGTGGCTGTGCCCATCCCTCACCG GGCCAACAAGAGTGACTACTGCAACCTGCCCCTGTATGCCAAGTCAGAGGCCTTCTTTCG AAAGGCAGATGGACGTGAGCCCTGCCCCGTGGTCCCACCCCGTGAGGCCTCCATCCGGAA CCTGGCTCGAACCTACCACACCCAGGCTCGCCACCTGACCCTGGACCCTGCCAGCAAGTC CTTGGGCCTTCCCCACCCAGGGGCCCCCGCTGCCGCCTCCACAGCCACCTTACCTCAGAG GACTCTGGCCATGCCAGCCCCCCCAGCCGGCACAGCCCCCCCAGCCCCCGGCCCCACCCC TGCTGAGCCACCCACCGCCCCCAGCGCTGCCCCTCCGGCCCCCAGCACCGAGCCTCCACG AGCCGGGGGCCCACACACCAAAATGGGGGGCTCCAGGGACTCGCTTCTCGAGATGAGCAC ATCGGGGGTAGGGAGGTCTCAGAAGCAGGGGGCCGGGGCCTACTCCAAATCCTACACCCT GGTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG ATCT
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