ARHGEF25 ORF clone pF1KSDB0010

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Flexi ORF Clone pF1KSDB0010

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Clone info.

Species

human

Clone Name

pF1KSDB0010 Sequence (with vector) Restriction Map

Product ID

FXC00977

HaloTag Clone PID

FHC00977

Original Clone PID

ORK00977

ORF Sequence

DNA sequence (1857 bp)
Protein sequence (619 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pF1KM Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384356]

Gene

KIBB0010

Symbol

ARHGEF25

Alias : GEFT, p63RhoGEF

Description

Rho guanine nucleotide exchange factor (GEF) 25, transcript variant 3

Original Clone ID

bm00447

Features of the cloned ORF sequence

Description

Length: 1857 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 619 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44931.1 9.8e-176 99.8 ARHGEF25 CCDS8947.1 5.3e-154 99.8 ARHGEF25 CCDS3883.1 1.3e-62 47.6 TRIO CCDS3028.1 4.1e-48 57.2 KALRN CCDS34124.1 1.3e-22 28.3 PLEKHG4B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 203 374 PF00621 Dbl homology (DH) domain HMMSmart IPR000219 203 374 SM00325 Dbl homology (DH) domain ProfileScan IPR000219 199 375 PS50010 Dbl homology (DH) domain IPR001849 387 505 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001104740 619 610215 100.0 100.0 NP_891992 580 610215 100.0 88.5 XP_016865292 2612 601893,617061 47.6 89.8 XP_016865291 2880 601893,617061 47.6 89.8 XP_011512412 3038 601893,617061 47.6 89.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KSDB0010 Download

>KIBB0010  1857 bp
ATGAAGCCCCCGGACCGCCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTCATGGGG
GGCATGCTGCGCGCATGCGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGCCCTCTA
GGGTTGGTGGGTACCGAGCCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGGGATCGC
GAACATGAGGTCCTCGCCGGGGCTCTGCAGCCCGAATCCTATTCCATTGCGGGCAGTGAG
GGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGC
CTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGG
TTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAGGCAGGA
CCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTG
ACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAG
ACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT
ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAG
ATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGC
CGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTC
TTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTCATCAAA
CACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAGCAT
GTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGC
CTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTG
CTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAA
GCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGA
TTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTC
TGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGGCGCGTC
TTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGAGGTGGA
ACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG
AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATC
CAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTG
GCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTAC
CAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGC
CCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGC
TCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTG
GATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTCTCTCCA
ACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTG

ORF Protein

Cloned ORF protein sequence for pF1KSDB0010 Download

>KIBB0010  619 aa
MKPPDRPAPGRTDRILGVMGGMLRACALPGQEGPPRRSPLGLVGTEPESERTEGDHRRDR
EHEVLAGALQPESYSIAGSEGSISASAASGLAAPSGPSSGLSSGPCSPGPPGPVSGLRRW
LDHSKHCLSVETEADSGQAGPYENWMLEPALATGEELPELTLLTTLLEGPGDKTQPPEEE
TLSQAPESEEEQKKKALERSMYVLSELVETEKMYVDDLGQIVEGYMATMAAQGVPESLRG
RDRIVFGNIQQIYEWHRDYFLQELQRCLKDPDWLAQLFIKHERRLHMYVVYCQNKPKSEH
VVSEFGDSYFEELRQQLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYNRAGMDTADLEQ
AVEVMCFVPKRCNDMMTLGRLRGFEGKLTAQGKLLGQDTFWVTEPEAGGLLSSRGRERRV
FLFEQIIIFSEALGGGVRGGTQPGYVYKNSIKVSCLGLEGNLQGDPCRFALTSRGPEGGI
QRYVLQAADPAISQAWIKHVAQILESQRDFLNALQSPIEYQRRESQTNSLGRPRGPGVGS
PGRIRLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQAPHDSPPVSP
TPKTPPCQARLAKLDEDEL

ORF+Vector

Nucleotide Sequence (with vector) for pF1KSDB0010 Download

>pF1KSDB0010  4996 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCTTTCGAAGGAGAT
AGAACCATGAAGCCCCCGGACCGCCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTC
ATGGGGGGCATGCTGCGCGCATGCGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGC
CCTCTAGGGTTGGTGGGTACCGAGCCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGG
GATCGCGAACATGAGGTCCTCGCCGGGGCTCTGCAGCCCGAATCCTATTCCATTGCGGGC
AGTGAGGGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGC
TCTGGCCTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGTGGCCTGAGG
AGATGGTTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAG
GCAGGACCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCG
GAACTGACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAA
GAGGAGACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAA
AGGAGTATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTG
GGGCAGATTGTGGAGGGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTT
CGAGGCCGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTATGAGTGGCACCGAGAC
TATTTCTTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAGCTATTC
ATCAAACACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCA
GAGCATGTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGG
CACCGCCTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAG
CTGCTGCTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTA
GAGCAAGCTGTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTG
GGGAGATTGCGGGGATTTGAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGAC
ACTTTCTGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTATCTTCCCGAGGTCGAGAGAGG
CGCGTCTTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTGAGA
GGTGGAACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTG
GAGGGGAACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGT
GGGATCCAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAG
CATGTGGCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATT
GAGTACCAGAGACGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTG
GGGAGCCCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATC
AATGGCTCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGAGCCCTCTTG
CCACTGGATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTC
TCTCCAACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAG
CTGTACGTAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGC
AGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGC
CACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTT
TTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAG
CTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCT
TGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGC
TTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGA
GCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTA
AACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAA
GAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCG
GCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCT
GATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGAC
CTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACG
ACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTG
CTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAA
GTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCA
TTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTT
GTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCC
AGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGC
TTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTG
GGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTT
GGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAG
CGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGA
AATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTA
TCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAG
CATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATAC
CAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACC
GGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGT
AGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCC
GTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA
CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTA
GGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTA
TTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA
TCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACG
CGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAG
TGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC
TAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATG
GTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCC
TCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTC
GGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGC
GGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGA
GATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGC
CTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGC
AGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCC
GATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG
AAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCT
CCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGG
GTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCT
GACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAA
ATATGTATCCGCTCAT