GRK3 ORF clone pF1KE3275

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Flexi ORF Clone pF1KE3275

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Clone info.

Species

human

Clone Name

pF1KE3275 Sequence (with vector) Restriction Map

Product ID

FXC27231

HaloTag Clone PID

FHC27231

ORF Sequence

DNA sequence (2064 bp)
Protein sequence (688 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3275

Symbol

GRK3

Alias : ADRBK2, BARK2

Description

G protein-coupled receptor kinase 3

Original Clone ID

cp93275

Features of the cloned ORF sequence

Description

Length: 2064 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 688 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13832.1 1.4e-153 100.0 GRK3 CCDS8156.1 3.6e-132 84.0 GRK2 CCDS43406.1 2.3e-33 38.9 GRK6 CCDS34303.1 2.4e-33 38.9 GRK6 CCDS47348.1 2.4e-33 38.9 GRK6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000239 171 183 PR00717 GPCR kinase IPR000239 230 248 PR00717 GPCR kinase IPR000239 468 485 PR00717 GPCR kinase IPR000239 493 506 PR00717 GPCR kinase IPR000239 507 524 PR00717 GPCR kinase HMMPfam IPR000342 55 174 PF00615 Regulator of G protein signalling IPR017442 192 453 PF00069 Serine/threonine-protein kinase-like domain IPR001849 559 651 PF00169 Pleckstrin homology domain HMMSmart IPR000342 54 175 SM00315 Regulator of G protein signalling IPR002290 191 453 SM00220 Serine/threonine-protein kinase domain IPR020635 191 453 SM00219 Tyrosine-protein kinase IPR000961 454 533 SM00133 AGC-kinase IPR001849 559 654 SM00233 Pleckstrin homology domain ProfileScan IPR000342 54 175 PS50132 Regulator of G protein signalling IPR000719 191 453 PS50011 Protein kinase IPR000961 454 521 PS51285 AGC-kinase IPR001849 558 652 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005151 688 109636 100.0 100.0 NP_001610 689 109635 84.0 100.0 XP_011543075 659 109635 83.4 94.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE3275 Download

>KIEE3275  2064 bp
ATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGC
AAGGCGACCCCGGCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGG
AGTGTGATGCAGAAGTACCTTGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAAT
CAGAAAATTGGTTTCTTGCTATTTAAAGATTTTTGTTTGAATGAAATTAATGAAGCTGTA
CCTCAGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAAAACTTGATAATGAGGAAGAC
CGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAAGGAACTTCTTTCCTGT
TCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAA
GTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGAC
ATTTTTCAAAAATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTT
GAATTAAATATCCATTTGACCATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGA
GGATTCGGGGAAGTTTATGGTTGCAGGAAAGCAGACACTGGAAAAATGTATGCAATGAAA
TGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAACATTAGCCTTAAATGAAAGA
ATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTATGACCTATGCC
TTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCAC
TACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAA
ATCATTCTGGGTCTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCA
GCAAATATTCTCTTGGATGAACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGC
GATTTTTCCAAAAAGAAGCCTCATGCGAGTGTTGGTACCCATGGGTACATGGCTCCCGAG
GTGCTGCAGAAGGGGACGGCCTATGACAGCAGTGCCGACTGGTTCTCCCTGGGCTGCATG
CTTTTCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAAAGACAAGCAT
GAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAA
CTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCAC
GGAGGCGGCTCACAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCAT
GTCTACTTACAAAAGTACCCACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCT
GATGCCTTTGATATTGGCTCATTTGATGAAGAGGATACCAAAGGGATTAAGCTACTTGAT
TGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCTCTGAACGCTGGCAGCAAGAA
GTAACGGAAACAGTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGAGGCCAGGAAG
AGAGCTAAAAATAAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATT
ATGCACGGGTACATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTAT
TTTTACCTCTTTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTA
CTGACAATGGAACAGATTCTCTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATT
TTGTTCAGAATAAAAGGAGGGAAACAATTTGTCTTGCAATGTGAGAGTGATCCAGAGTTT
GTGCAGTGGAAGAAAGAGTTGAACGAAACCTTCAAGGAGGCCCAGCGGCTATTGCGTCGT
GCCCCGAAGTTCCTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAAGCCATCCCTC
TGTCACAGAAACAGCAACGGCCTC

ORF Protein

Cloned ORF protein sequence for pF1KE3275 Download

>KIEE3275  688 aa
MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFN
QKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSC
SHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNV
ELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNER
IMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATE
IILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPE
VLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPE
LKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAA
DAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARK
RAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNL
LTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRR
APKFLNKPRSGTVELPKPSLCHRNSNGL

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE3275 Download

>pF1KE3275  5177 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGCGGACCTG
GAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGCAAGGCGACCCCG
GCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAG
AAGTACCTTGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGT
TTCTTGCTATTTAAAGATTTTTGTTTGAATGAAATTAATGAAGCTGTACCTCAGGTGAAG
TTTTATGAAGAGATAAAGGAATATGAAAAACTTGATAATGAGGAAGACCGCCTTTGCAGA
AGTCGACAAATTTATGATGCCTACATCATGAAGGAACTTCTTTCCTGTTCACATCCTTTC
TCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAAGTGACATCAACT
CTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGACATTTTTCAAAAA
TTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGAATTAAATATC
CATTTGACCATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGAGGATTCGGGGAA
GTTTATGGTTGCAGGAAAGCAGACACTGGAAAAATGTATGCAATGAAATGCTTAGATAAG
AAGAGGATCAAAATGAAACAAGGAGAAACATTAGCCTTAAATGAAAGAATCATGTTGTCT
CTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTATGACCTATGCCTTCCATACCCCA
GATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCACTACCACCTTTCA
CAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAAATCATTCTGGGT
CTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCAGCAAATATTCTC
TTGGATGAACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGCGATTTTTCCAAA
AAGAAGCCTCATGCGAGTGTTGGTACCCATGGGTACATGGCTCCCGAGGTGCTGCAGAAG
GGGACGGCCTATGACAGCAGTGCCGACTGGTTCTCCCTGGGCTGCATGCTTTTCAAACTT
CTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAAAGACAAGCATGAAATTGACCGA
ATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAACTGAAGTCCCTT
TTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCACGGAGGCGGCTCA
CAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCATGTCTACTTACAA
AAGTACCCACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCTGATGCCTTTGAT
ATTGGCTCATTTGATGAAGAGGATACCAAAGGGATTAAGCTACTTGATTGCGACCAAGAA
CTCTACAAGAACTTCCCTTTGGTCATCTCTGAACGCTGGCAGCAAGAAGTAACGGAAACA
GTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGAGGCCAGGAAGAGAGCTAAAAAT
AAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATTATGCACGGGTAC
ATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTATTTTTACCTCTTT
CCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTACTGACAATGGAA
CAGATTCTCTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATTTTGTTCAGAATA
AAAGGAGGGAAACAATTTGTCTTGCAATGTGAGAGTGATCCAGAGTTTGTGCAGTGGAAG
AAAGAGTTGAACGAAACCTTCAAGGAGGCCCAGCGGCTATTGCGTCGTGCCCCGAAGTTC
CTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAAGCCATCCCTCTGTCACAGAAAC
AGCAACGGCCTCGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACC
TGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC
TGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGG
TTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTC
TAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTC
CCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACT
GGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCG
TGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAA
GTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGAT
CAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCT
CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGC
TCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACC
GACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCC
ACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGG
CTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAG
AAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGC
CCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGT
CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTC
GCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCC
TGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGG
CTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAG
CTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCG
CAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCG
AAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT
ATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGC
CAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGA
GCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATA
CCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTAC
CGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG
TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCC
CGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAG
ACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT
AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT
ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTG
ATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTAC
GCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCA
GTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC
CTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGAT
GGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACC
CTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTT
CGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAG
CGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCG
AGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAG
CCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGG
CAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGC
CGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAAC
GAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC
TCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG
GGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCC
TGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCAT