PDE5A ORF clone pF1KE0334

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Flexi ORF Clone pF1KE0334

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Clone info.

Species

human

Clone Name

pF1KE0334 Sequence (with vector) Restriction Map

Product ID

FXC10958

HaloTag Clone PID

FHC10958

ORF Sequence

DNA sequence (2625 bp)
Protein sequence (875 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527373]

Gene

KIEE0334

Symbol

PDE5A

Alias : CGB-PDE, CN5A, PDE5

Description

phosphodiesterase 5A, cGMP-specific, transcript variant 1

Original Clone ID

oc01034

Features of the cloned ORF sequence

Description

Length: 2625 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 875 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3713.1 0 99.7 PDE5A CCDS34055.1 0 99.8 PDE5A CCDS33334.1 7.3e-105 43.3 PDE11A CCDS42785.1 6.1e-89 44.1 PDE11A CCDS46459.1 1e-76 42.9 PDE11A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023088 608 621 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 639 652 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 653 668 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 679 695 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 760 773 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 777 793 PR00387 3'5'-cyclic nucleotide phosphodiesterase HMMPfam IPR003018 167 312 PF01590 GAF IPR003018 346 503 PF01590 GAF IPR002073 612 850 PF00233 3'5'-cyclic nucleotide phosphodiesterase HMMSmart IPR003018 164 324 SM00065 GAF IPR003018 346 513 SM00065 GAF IPR003607 610 778 SM00471 Metal-dependent phosphohydrolase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001074 875 603310 99.7 100.0 NP_236914 833 603310 99.8 94.4 NP_246273 823 603310 99.8 94.1 NP_058649 933 604961,610475 43.3 84.7 XP_016865685 709 610652,616921,616922 32.4 80.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE0334 Download

>KIEE0334  2625 bp
ATGGAGCGGGCCGGCCCCAGCTTCGGGCAGCAGCGACAGCAGCAGCAGCCCCAGCAGCAG
AAGCAGCAGCAGAGGGATCAGGACTCGGTCGAAGCATGGCTGGACGGTCACTGGGACTTT
ACCTTCTCATACTTTGTTAGAAAAGCCACCAGAGAAATGGTCAATGCATGGTTTGCTGAG
AGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCT
TGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGTCCCTGGAACACCAACCAGGAAA
ATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGA
ACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGG
TTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCT
AGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATA
TCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTT
ATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGT
ATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTG
AACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGC
TACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGT
GTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAA
AAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTAT
GAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTCGACCTTGCTAGTTTAATT
TTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCT
TTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTCTTTT
TCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGG
GAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTACGTCAAAAATACTATGGAA
CCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACA
GGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAG
AAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAG
GTTAAACCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGC
TTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATG
GTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTA
CAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGC
TTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACT
GACCTCAACCTTGTGCAGAACTTCCGGATGAAACATGAGGTTCTTTGCAGATGGATTTTA
AGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAAT
ACAGCTCAGTGCATGTTTGCCGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGAC
CTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGGTGTG
AATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATC
ATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATT
CTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATT
TTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGA
AAAAATCAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATG
ACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCGGAA
CTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACATAGAA
CCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTC
ATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTC
CCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAG
GAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAAC

ORF Protein

Cloned ORF protein sequence for pF1KE0334 Download

>KIEE0334  875 aa
MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDGHWDFTFSYFVRKATREMVNAWFAE
RVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEG
TVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLI
SADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPL
NIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDE
KDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIIS
FMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTME
PLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGK
VKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETREL
QSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFRMKHEVLCRWIL
SVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGV
NNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAI
LATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAE
LVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCF
PLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE0334 Download

>pF1KE0334  5738 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGAGCGGGCC
GGCCCCAGCTTCGGGCAGCAGCGACAGCAGCAGCAGCCCCAGCAGCAGAAGCAGCAGCAG
AGGGATCAGGACTCGGTCGAAGCATGGCTGGACGGTCACTGGGACTTTACCTTCTCATAC
TTTGTTAGAAAAGCCACCAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACC
ATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAG
CAGAGTCCTCGTGCAGATAACAGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCT
GAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTC
CTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGAT
GAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGAT
GTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGC
TATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTC
TTTGATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAA
TGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGAT
GCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAA
AGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGTGTAGCCCAGGCC
ATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTGCT
GCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTG
CTGGAGAACAAGAGAAATCAGGTGCTGCTCGACCTTGCTAGTTTAATTTTTGAAGAACAA
CAATCATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTG
CAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTCTTTTTCTAGTGTGTTT
CACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGGGAACATGATGCA
AACAAAATCAATTACATGTATGCTCAGTACGTCAAAAATACTATGGAACCACTTAATATC
CCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAAAC
CAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTT
ATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAACCTTTC
AACCGAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGGATCCAG
AACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATGGTCACATTGGAG
GTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTACAGTCGTTAGCG
GCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGCTTCAGTGACTTT
GAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACCTT
GTGCAGAACTTCCGGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAG
AATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGC
ATGTTTGCCGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTT
GCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGGTGTGAATAACTCTTAC
ATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATCATGGAACACCAT
CATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATTCTCAGTGGCCTC
TCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAGAC
CTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTC
AATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGAT
CTTTCTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCGGAACTTGTAGCAACT
GAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACATAGAACCCACTGATCTA
ATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATC
TGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTCCCTTTGCTAGAT
GGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGCTG
ATTAATGGGGAAAGCGGCCAGGCCAAGCGGAACGTTTAAACGAATTCGAGCTCGGTACCC
GGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCG
AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGC
CTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGC
TGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTT
TCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGG
TCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCG
CCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGT
AAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGG
CGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAA
GATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGG
GCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC
CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCA
GCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC
ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCA
TCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCAT
ACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCA
CGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGG
CTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTC
GTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCT
GGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT
ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTAC
GGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTC
TGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCA
CTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTG
AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCA
TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAA
CCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC
TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC
GCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT
GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG
TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAG
GATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA
CGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGG
AAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTT
TGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTT
TTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAG
ATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGC
TGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGT
GGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCA
GGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTG
ATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTAC
TGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGT
AGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGAT
AAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTC
AGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAA
CTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCT
GTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACG
TTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATC
AAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT