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SpecieshumanProduct IDFXC27058Cloning SiteSgfI-PmeIGenBank AccessionGeneKIBB4370SymbolARFGEF2
Alias : BIG2, PVNH2, dJ1164I10.1DescriptionADP ribosylation factor guanine nucleotide exchange factor 2Original Clone IDcp01713NoteWe obtained this clone using the E.coli strain CopyCutterEPI400 at 25 deg C.This plasmid seems unstable at 37 deg C.
Length: 5355 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1785 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS13411.1 0 100.0 ARFGEF2 CCDS6199.1 0 75.0 ARFGEF1 CCDS32754.1 7.9e-34 51.6 CYTH1 CCDS42392.2 7.9e-34 51.6 CYTH1 CCDS5346.1 3.4e-32 50.5 CYTH3
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition HMMPfam IPR000904 641 826 PF01369 SEC7-like IPR015403 1166 1249 PF09324 Domain of unknown function DUF1981 HMMSmart IPR000904 640 827 SM00222 SEC7-like ProfileScan IPR000904 636 825 PS50190 SEC7-like
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006411 1785 605371,608097 100.0 100.0 XP_005260309 1784 605371,608097 99.9 100.0 XP_005251192 1843 604141 75.0 98.2 XP_011515744 1649 604141 74.4 88.8 XP_005251193 1800 604141 74.7 95.7 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pF1KB4370 Download>KIBB4370 5355 bp ATGCAGGAGAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCC GACAAGGAGGTGAAGCGGCCCCAGCACTCCCAGCTGCGCAGGGCCTGCCAGGTGGCGCTC GATGAAATTAAAGCAGAAATAGAAAAGCAGAGGCTTGGCACTGCTGCACCACCAAAGGCA AACTTCATTGAAGCTGACAAGTATTTTCTTCCATTCGAGCTAGCTTGCCAGTCCAAGTCC CCAAGGGTAGTCAGCACATCCCTTGACTGCTTGCAGAAACTCATCGCATACGGGCACATC ACTGGCAACGCCCCTGACAGTGGAGCCCCTGGGAAGCGGCTGATCGACAGAATTGTTGAA ACCATTTGCAGTTGTTTTCAGGGCCCTCAGACTGATGAAGGGGTTCAGTTACAAATAATT AAGGCTCTTCTGACTGCAGTGACTTCCCCACACATTGAAATTCATGAGGGTACTATCCTG CAGACAGTGAGAACATGTTACAATATCTATTTGGCCAGCAAAAATCTCATCAATCAAACC ACTGCCAAGGCTACCCTTACTCAGATGCTGAACGTCATTTTCACCCGCATGGAAAACCAA GTGTTGCAGGAGGCCAGAGAACTGGAAAAACCAATCCAGTCAAAACCCCAGTCCCCTGTG ATCCAAGCTGCAGCAGTATCCCCAAAGTTCGTTCGTTTGAAGCACAGTCAGGCACAAAGC AAACCAACAACTCCCGAAAAAACAGATTTAACCAACGGTGAACATGCCAGGAGTGATTCT GGAAAAGTAAGCACAGAAAATGGAGACGCACCCAGAGAAAGAGGCTCATCACTGTCAGGG ACTGATGACGGAGCCCAGGAGGTGGTGAAGGACATCTTGGAAGATGTAGTCACATCTGCC ATTAAAGAAGCAGCGGAAAAGCATGGTCTGACAGAACCTGAGAGAGTTCTAGGTGAACTG GAGTGCCAGGAATGTGCTATTCCCCCAGGAGTTGATGAAAACTCACAGACCAACGGGATA GCCGATGACAGGCAGTCCTTGTCGTCAGCAGATAATCTGGAATCGGATGCACAAGGACAT CAAGTGGCTGCCAGGTTCTCCCACGTTCTGCAGAAGGATGCCTTCCTTGTGTTCCGCTCC CTGTGCAAGCTGTCCATGAAACCCCTTGGTGAAGGCCCTCCAGACCCAAAATCCCATGAG CTGCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCTGTGTTGCAAAATGCTGGCCCC GTATTCAGGACTCACGAGATGTTCATCAATGCAATCAAGCAATATCTCTGTGTGGCCTTG TCCAAAAACGGCGTCTCTTCAGTGCCTGATGTCTTTGAGCTCTCTCTTGCCATTTTTCTT ACTCTTCTTTCAAACTTTAAAATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAG ATTTTCCTGAACATTTTAGAAACATCAACAAGTTCTTTTGAGCACAGGTGGATGGTCATT CAGACTCTGACGAGGATCTGTGCAGATGCCCAGTGTGTTGTGGATATTTATGTCAACTAC GACTGTGATTTAAATGCTGCTAACATTTTTGAGCGCCTTGTAAATGATTTATCCAAAATT GCTCAGGGAAGAAGTGGACATGAGCTGGGAATGACACCTCTGCAGGAGCTCAGCCTGAGG AAGAAAGGCCTGGAGTGCCTCGTGTCCATTCTCAAGTGCATGGTGGAGTGGAGCAAAGAC CTGTATGTGAATCCCAACCACCAGACCAGCCTCGGTCAGGAGAGGCTCACGGATCAGGAA ATAGGGGATGGGAAAGGCCTTGACATGGCAAGACGGTGTAGTGTGACGTCCATGGAGTCC ACAGTGTCCTCGGGGACCCAGACAACTGTTCAGGATGACCCTGAGCAATTTGAGGTCATC AAGCAACAAAAAGAAATCATTGAACACGGCATCGAGCTGTTCAACAAGAAACCCAAGAGG GGGATCCAGTTTCTCCAGGAGCAGGGCATGCTGGGAACGTCAGTTGAAGACATAGCCCAA TTCCTGCACCAGGAGGAGCGCCTGGATTCCACCCAAGTAGGCGATTTTCTGGGAGATAGC GCAAGGTTCAACAAGGAGGTGATGTATGCCTACGTGGACCAACTTGACTTCTGTGAAAAA GAATTTGTCTCAGCCCTGCGGACATTCCTAGAAGGTTTCCGCCTACCTGGAGAAGCCCAA AAGATTGACCGATTAATGGAGAAGTTTGCCGCAAGATACATAGAATGCAACCAAGGGCAA ACTCTGTTTGCTAGTGCTGACACTGCTTATGTCCTAGCGTATTCAATTATTATGCTGACT ACAGACTTGCACAGTCCTCAGGTAAAAAATAAAATGACGAAAGAGCAGTATATTAAAATG AATCGGGGTATCAATGATAGTAAAGATCTGCCAGAAGAGTATCTCTCAAGCATCTATGAA GAGATAGAAGGCAAGAAAATTGCAATGAAAGAAACAAAAGAGCTAACGATTGCAACCAAA TCTACTAAGCAGAATGTAGCTAGTGAAAAGCAGCGGCGGCTGCTGTACAACTTAGAGATG GAGCAAATGGCTAAAACAGCCAAAGCTCTGATGGAGGCTGTGAGCCATGCCAAAGCCCCG TTTACCAGTGCCACTCACCTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCA CTATTGGCAGCCTACAGCATCGGACTCCAGAACTGTGATGACACTGAAGTGGCCTCCTTG TGTTTGGAAGGCATCCGATGTGCAATCCGAATCGCCTGCATCTTTGGAATGCAGCTGGAA CGAGATGCCTATGTTCAGGCTCTTGCTCGCTTCTCCCTACTCACAGCCAGCTCCAGCATC ACAGAAATGAAGCAGAAAAACATCGACACCATTAAGACGCTTATCACAGTGGCTCACACC GATGGCAACTACCTTGGGAATTCCTGGCATGAGATCTTGAAATGCATCAGCCAGCTGGAG CTCGCTCAGCTGATAGGAACCGGTGTGAAGACGCGCTACCTGTCTGGATCTGGGCGTGAA AGAGAAGGGAGCCTGAAGGGCCACACATTGGCAGGAGAAGAGTTCATGGGCCTTGGCCTC GGTAATTTGGTGAGTGGCGGAGTGGATAAAAGACAGATGGCCAGCTTCCAAGAATCGGTT GGTGAGACCAGCTCGCAGAGTGTGGTTGTAGCTGTGGACAGGATTTTTACTGGGTCTACC AGACTGGATGGAAATGCAATAGTTGACTTTGTCCGCTGGCTGTGTGCTGTGTCCATGGAT GAACTGGCTTCCCCCCACCATCCTCGCATGTTCAGCTTGCAGAAGATTGTGGAGATATCA TACTACAACATGAATCGGATCCGACTACAGTGGTCTCGAATATGGCATGTGATTGGAGAT CACTTCAATAAGGTTGGCTGCAACCCTAATGAAGATGTGGCTATCTTTGCTGTTGACTCA TTAAGGCAACTCTCCATGAAGTTTCTTGAGAAGGGTGAATTAGCCAACTTCCGTTTCCAG AAAGATTTTCTGAGGCCCTTTGAGCATATTATGAAGAAAAACAGGTCTCCCACCATCCGG GACATGGCGATCCGCTGCATTGCCCAGATGGTGAACTCCCAGGCGGCCAACATCCGCTCA GGTTGGAAGAACATCTTTGCCGTGTTCCACCAGGCAGCCTCTGATCATGATGGGAACATT GTGGAGCTGGCCTTCCAGACCACTTGCCACATTGTCACAACTATTTTCCAGCACCATTTT CCTGCAGCCATCGATTCCTTTCAGGATGCTGTGAAGTGCTTATCAGAGTTCGCCTGCAAC GCCGCTTTCCCTGACACGAGCATGGAAGCGATTCGGCTCATCCGCTTCTGTGGCAAATAC GTCTCTGAGAGGCCTCGGGTGCTACAAGAATACACAAGTGATGACATGAATGTAGCTCCT GGTGACAGAGTCTGGGTCCGAGGCTGGTTCCCCATCTTATTCGAACTCTCCTGCATCATT AATAGATGCAAGTTAGATGTACGAACAAGGGGACTCACAGTCATGTTTGAGATCATGAAG AGCTATGGCCACACCTTTGAAAAGCACTGGTGGCAGGACCTGTTCAGAATCGTGTTTCGG ATTTTTGACAATATGAAACTCCCTGAGCAACTGTCAGAGAAATCTGAGTGGATGACAACA ACCTGCAATCACGCACTTTATGCTATTTGTGATGTTTTTACCCAGTTTTATGAAGCTTTG AATGAAGTTCTTCTTTCTGATGTATTTGCACAATTGCAGTGGTGTGTCAAACAAGATAAT GAACAGTTGGCGCGATCAGGTACAAATTGCTTAGAAAACTTAGTAATATCCAATGGAGAG AAATTCAGTCCTGAAGTCTGGGATGAAACCTGCAACTGTATGTTGGATATTTTCAAAACA ACCATCCCACATGTTTTGCTGACATGGAGACCTGTAGGAATGGAGGAAGATTCATCAGAA AAGCATTTGGATGTGGATCTGGACCGCCAGTCTTTAAGCAGCATAGATAAAAATCCCTCT GAGAGGGGACAGAGCCAGCTCTCTAACCCAACAGATGACAGCTGGAAGGGTAGACCATAC GCAAATCAGAAACTGTTTGCCAGCCTCCTCATCAAGTGTGTGGTCCAGTTGGAATTGATA CAGACCATTGACAACATTGTGTTCTACCCTGCGACGAGCAAAAAGGAGGATGCAGAGCAC ATGGTTGCCGCCCAGCAAGACACGCTGGATGCAGATATCCACATAGAGACGGAGGATCAG GGCATGTATAAGTACATGTCTTCCCAGCACCTCTTCAAGCTGTTGGACTGTTTGCAGGAA TCCCATTCATTCTCAAAGGCCTTCAACTCCAATTACGAGCAGCGGACTGTCCTGTGGCGA GCAGGTTTTAAGGGCAAGTCTAAACCCAATCTTCTAAAACAAGAAACCAGCAGCCTGGCC TGTTGTTTGAGGATCCTGTTTCGAATGTATGTTGATGAGAACCGCAGGGATTCCTGGGAA GAAATACAGCAGAGACTTTTAACTGTTTGCAGTGAAGCTCTTGCCTATTTCATCACTGTG AATTCTGAGAGCCATCGGGAGGCCTGGACAAGTCTCTTGTTGTTACTTCTAACTAAAACC CTCAAAATAAATGATGAAAAGTTCAAAGCACATGCTTCAATGTACTACCCCTACTTGTGT GAAATTATGCAGTTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGGAAGTTCTTCCTA CGGATAGGTGTTGTGTATAAGATATGGATACCAGAAGAGCCATCACAGGTACCAGCAGCA CTGTCACCAGTGTGG
Cloned ORF protein sequence for pF1KB4370 Download>KIBB4370 1785 aa MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKA NFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVE TICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQT TAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQS KPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSA IKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGH QVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGP VFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKE IFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKI AQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQE IGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKR GIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEK EFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLT TDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATK STKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTP LLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSI TEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGRE REGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGST RLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGD HFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIR DMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHF PAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAP GDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFR IFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDN EQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSE KHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELI QTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQE SHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWE EIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLC EIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW
Nucleotide Sequence (with vector) for pF1KB4370 Download>pF1KB4370 8468 bp GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGCAGGAGAGC CAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCCGACAAGGAGGTG AAGCGGCCCCAGCACTCCCAGCTGCGCAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAA GCAGAAATAGAAAAGCAGAGGCTTGGCACTGCTGCACCACCAAAGGCAAACTTCATTGAA GCTGACAAGTATTTTCTTCCATTCGAGCTAGCTTGCCAGTCCAAGTCCCCAAGGGTAGTC AGCACATCCCTTGACTGCTTGCAGAAACTCATCGCATACGGGCACATCACTGGCAACGCC CCTGACAGTGGAGCCCCTGGGAAGCGGCTGATCGACAGAATTGTTGAAACCATTTGCAGT TGTTTTCAGGGCCCTCAGACTGATGAAGGGGTTCAGTTACAAATAATTAAGGCTCTTCTG ACTGCAGTGACTTCCCCACACATTGAAATTCATGAGGGTACTATCCTGCAGACAGTGAGA ACATGTTACAATATCTATTTGGCCAGCAAAAATCTCATCAATCAAACCACTGCCAAGGCT ACCCTTACTCAGATGCTGAACGTCATTTTCACCCGCATGGAAAACCAAGTGTTGCAGGAG GCCAGAGAACTGGAAAAACCAATCCAGTCAAAACCCCAGTCCCCTGTGATCCAAGCTGCA GCAGTATCCCCAAAGTTCGTTCGTTTGAAGCACAGTCAGGCACAAAGCAAACCAACAACT CCCGAAAAAACAGATTTAACCAACGGTGAACATGCCAGGAGTGATTCTGGAAAAGTAAGC ACAGAAAATGGAGACGCACCCAGAGAAAGAGGCTCATCACTGTCAGGGACTGATGACGGA GCCCAGGAGGTGGTGAAGGACATCTTGGAAGATGTAGTCACATCTGCCATTAAAGAAGCA GCGGAAAAGCATGGTCTGACAGAACCTGAGAGAGTTCTAGGTGAACTGGAGTGCCAGGAA TGTGCTATTCCCCCAGGAGTTGATGAAAACTCACAGACCAACGGGATAGCCGATGACAGG CAGTCCTTGTCGTCAGCAGATAATCTGGAATCGGATGCACAAGGACATCAAGTGGCTGCC AGGTTCTCCCACGTTCTGCAGAAGGATGCCTTCCTTGTGTTCCGCTCCCTGTGCAAGCTG TCCATGAAACCCCTTGGTGAAGGCCCTCCAGACCCAAAATCCCATGAGCTGCGTTCCAAG GTGGTTTCCCTGCAGCTGCTCCTCTCTGTGTTGCAAAATGCTGGCCCCGTATTCAGGACT CACGAGATGTTCATCAATGCAATCAAGCAATATCTCTGTGTGGCCTTGTCCAAAAACGGC GTCTCTTCAGTGCCTGATGTCTTTGAGCTCTCTCTTGCCATTTTTCTTACTCTTCTTTCA AACTTTAAAATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTGAAC ATTTTAGAAACATCAACAAGTTCTTTTGAGCACAGGTGGATGGTCATTCAGACTCTGACG AGGATCTGTGCAGATGCCCAGTGTGTTGTGGATATTTATGTCAACTACGACTGTGATTTA AATGCTGCTAACATTTTTGAGCGCCTTGTAAATGATTTATCCAAAATTGCTCAGGGAAGA AGTGGACATGAGCTGGGAATGACACCTCTGCAGGAGCTCAGCCTGAGGAAGAAAGGCCTG GAGTGCCTCGTGTCCATTCTCAAGTGCATGGTGGAGTGGAGCAAAGACCTGTATGTGAAT CCCAACCACCAGACCAGCCTCGGTCAGGAGAGGCTCACGGATCAGGAAATAGGGGATGGG AAAGGCCTTGACATGGCAAGACGGTGTAGTGTGACGTCCATGGAGTCCACAGTGTCCTCG GGGACCCAGACAACTGTTCAGGATGACCCTGAGCAATTTGAGGTCATCAAGCAACAAAAA GAAATCATTGAACACGGCATCGAGCTGTTCAACAAGAAACCCAAGAGGGGGATCCAGTTT CTCCAGGAGCAGGGCATGCTGGGAACGTCAGTTGAAGACATAGCCCAATTCCTGCACCAG GAGGAGCGCCTGGATTCCACCCAAGTAGGCGATTTTCTGGGAGATAGCGCAAGGTTCAAC AAGGAGGTGATGTATGCCTACGTGGACCAACTTGACTTCTGTGAAAAAGAATTTGTCTCA GCCCTGCGGACATTCCTAGAAGGTTTCCGCCTACCTGGAGAAGCCCAAAAGATTGACCGA TTAATGGAGAAGTTTGCCGCAAGATACATAGAATGCAACCAAGGGCAAACTCTGTTTGCT AGTGCTGACACTGCTTATGTCCTAGCGTATTCAATTATTATGCTGACTACAGACTTGCAC AGTCCTCAGGTAAAAAATAAAATGACGAAAGAGCAGTATATTAAAATGAATCGGGGTATC AATGATAGTAAAGATCTGCCAGAAGAGTATCTCTCAAGCATCTATGAAGAGATAGAAGGC AAGAAAATTGCAATGAAAGAAACAAAAGAGCTAACGATTGCAACCAAATCTACTAAGCAG AATGTAGCTAGTGAAAAGCAGCGGCGGCTGCTGTACAACTTAGAGATGGAGCAAATGGCT AAAACAGCCAAAGCTCTGATGGAGGCTGTGAGCCATGCCAAAGCCCCGTTTACCAGTGCC ACTCACCTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCACTATTGGCAGCC TACAGCATCGGACTCCAGAACTGTGATGACACTGAAGTGGCCTCCTTGTGTTTGGAAGGC ATCCGATGTGCAATCCGAATCGCCTGCATCTTTGGAATGCAGCTGGAACGAGATGCCTAT GTTCAGGCTCTTGCTCGCTTCTCCCTACTCACAGCCAGCTCCAGCATCACAGAAATGAAG CAGAAAAACATCGACACCATTAAGACGCTTATCACAGTGGCTCACACCGATGGCAACTAC CTTGGGAATTCCTGGCATGAGATCTTGAAATGCATCAGCCAGCTGGAGCTCGCTCAGCTG ATAGGAACCGGTGTGAAGACGCGCTACCTGTCTGGATCTGGGCGTGAAAGAGAAGGGAGC CTGAAGGGCCACACATTGGCAGGAGAAGAGTTCATGGGCCTTGGCCTCGGTAATTTGGTG AGTGGCGGAGTGGATAAAAGACAGATGGCCAGCTTCCAAGAATCGGTTGGTGAGACCAGC TCGCAGAGTGTGGTTGTAGCTGTGGACAGGATTTTTACTGGGTCTACCAGACTGGATGGA AATGCAATAGTTGACTTTGTCCGCTGGCTGTGTGCTGTGTCCATGGATGAACTGGCTTCC CCCCACCATCCTCGCATGTTCAGCTTGCAGAAGATTGTGGAGATATCATACTACAACATG AATCGGATCCGACTACAGTGGTCTCGAATATGGCATGTGATTGGAGATCACTTCAATAAG GTTGGCTGCAACCCTAATGAAGATGTGGCTATCTTTGCTGTTGACTCATTAAGGCAACTC TCCATGAAGTTTCTTGAGAAGGGTGAATTAGCCAACTTCCGTTTCCAGAAAGATTTTCTG AGGCCCTTTGAGCATATTATGAAGAAAAACAGGTCTCCCACCATCCGGGACATGGCGATC CGCTGCATTGCCCAGATGGTGAACTCCCAGGCGGCCAACATCCGCTCAGGTTGGAAGAAC ATCTTTGCCGTGTTCCACCAGGCAGCCTCTGATCATGATGGGAACATTGTGGAGCTGGCC TTCCAGACCACTTGCCACATTGTCACAACTATTTTCCAGCACCATTTTCCTGCAGCCATC GATTCCTTTCAGGATGCTGTGAAGTGCTTATCAGAGTTCGCCTGCAACGCCGCTTTCCCT GACACGAGCATGGAAGCGATTCGGCTCATCCGCTTCTGTGGCAAATACGTCTCTGAGAGG CCTCGGGTGCTACAAGAATACACAAGTGATGACATGAATGTAGCTCCTGGTGACAGAGTC TGGGTCCGAGGCTGGTTCCCCATCTTATTCGAACTCTCCTGCATCATTAATAGATGCAAG TTAGATGTACGAACAAGGGGACTCACAGTCATGTTTGAGATCATGAAGAGCTATGGCCAC ACCTTTGAAAAGCACTGGTGGCAGGACCTGTTCAGAATCGTGTTTCGGATTTTTGACAAT ATGAAACTCCCTGAGCAACTGTCAGAGAAATCTGAGTGGATGACAACAACCTGCAATCAC GCACTTTATGCTATTTGTGATGTTTTTACCCAGTTTTATGAAGCTTTGAATGAAGTTCTT CTTTCTGATGTATTTGCACAATTGCAGTGGTGTGTCAAACAAGATAATGAACAGTTGGCG CGATCAGGTACAAATTGCTTAGAAAACTTAGTAATATCCAATGGAGAGAAATTCAGTCCT GAAGTCTGGGATGAAACCTGCAACTGTATGTTGGATATTTTCAAAACAACCATCCCACAT GTTTTGCTGACATGGAGACCTGTAGGAATGGAGGAAGATTCATCAGAAAAGCATTTGGAT GTGGATCTGGACCGCCAGTCTTTAAGCAGCATAGATAAAAATCCCTCTGAGAGGGGACAG AGCCAGCTCTCTAACCCAACAGATGACAGCTGGAAGGGTAGACCATACGCAAATCAGAAA CTGTTTGCCAGCCTCCTCATCAAGTGTGTGGTCCAGTTGGAATTGATACAGACCATTGAC AACATTGTGTTCTACCCTGCGACGAGCAAAAAGGAGGATGCAGAGCACATGGTTGCCGCC CAGCAAGACACGCTGGATGCAGATATCCACATAGAGACGGAGGATCAGGGCATGTATAAG TACATGTCTTCCCAGCACCTCTTCAAGCTGTTGGACTGTTTGCAGGAATCCCATTCATTC TCAAAGGCCTTCAACTCCAATTACGAGCAGCGGACTGTCCTGTGGCGAGCAGGTTTTAAG GGCAAGTCTAAACCCAATCTTCTAAAACAAGAAACCAGCAGCCTGGCCTGTTGTTTGAGG ATCCTGTTTCGAATGTATGTTGATGAGAACCGCAGGGATTCCTGGGAAGAAATACAGCAG AGACTTTTAACTGTTTGCAGTGAAGCTCTTGCCTATTTCATCACTGTGAATTCTGAGAGC CATCGGGAGGCCTGGACAAGTCTCTTGTTGTTACTTCTAACTAAAACCCTCAAAATAAAT GATGAAAAGTTCAAAGCACATGCTTCAATGTACTACCCCTACTTGTGTGAAATTATGCAG TTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGGAAGTTCTTCCTACGGATAGGTGTT GTGTATAAGATATGGATACCAGAAGAGCCATCACAGGTACCAGCAGCACTGTCACCAGTG TGGGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCAT GCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGC TGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCT GAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCG CCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCA GATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTA CGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCA AAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGG ATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACA GGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCT TGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCC GCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCC GGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGC GTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTG GGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCC ATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGAC CACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGAT CAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTC AAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCG AATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTG GCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGC GAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATC GCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCG ACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA ATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCG TAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAA AAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT TCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCT CAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC CGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTT ATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGC TACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTAT CTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAA ACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA AAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA AAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCT TTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACC ATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTG CATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTT TGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTA CAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACC GCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACA GATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGC GGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAG TGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTC AGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTA GGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGG CAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATG GCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTAT CCGCTCAT
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