EIF4A1 ORF clone pF1KB3435

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KOP Flexi HaloTag ORF List search

Flexi ORF Clone pF1KB3435

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Clone info.

Species

human

Clone Name

pF1KB3435 Sequence (with vector) Restriction Map

Product ID

FXC01735

HaloTag Clone PID

FHC01735

ORF Sequence

DNA sequence (1218 bp)
Protein sequence (406 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385527]

Gene

KIBB3435

Symbol

EIF4A1

Alias : DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I

Description

eukaryotic translation initiation factor 4A1, transcript variant 1

Original Clone ID

ha01720

Features of the cloned ORF sequence

Description

Length: 1218 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 406 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11113.1 2.4e-166 100.0 EIF4A1 CCDS3282.1 4.9e-153 89.7 EIF4A2 CCDS58511.1 2.5e-135 99.4 EIF4A1 CCDS11767.1 2.2e-111 66.7 EIF4A3 CCDS44751.1 1e-55 38.7 DDX6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 57 222 PF00270 DNA/RNA helicase IPR001650 292 367 PF00271 Helicase HMMSmart IPR014001 51 249 SM00487 DEAD-like helicase IPR001650 286 367 SM00490 Helicase ProfileScan IPR014014 32 60 PS51195 RNA helicase IPR014001 63 234 PS51192 DEAD-like helicase IPR001650 245 406 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001407 406 602641 100.0 100.0 NP_001958 407 601102 89.7 100.0 NP_001191439 347 602641 99.4 82.3 NP_055555 411 268305,608546 66.7 100.0 XP_011523824 390 268305,608546 63.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KB3435 Download

>KIBB3435  1218 bp
ATGTCTGCGAGCCAGGATTCCCGATCCAGAGACAATGGCCCCGATGGGATGGAGCCCGAA
GGCGTCATCGAGAGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCG
GAGTCCCTTCTCCGTGGCATCTACGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAG
CGAGCCATTCTACCTTGTATCAAGGGTTATGATGTGATTGCTCAAGCCCAATCTGGGACT
GGGAAAACGGCCACATTTGCCATATCGATTCTGCAGCAGATTGAATTAGATCTAAAAGCC
ACCCAGGCCTTGGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACAGAAGGTGGTC
ATGGCACTAGGAGACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTG
CGTGCTGAGGTGCAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGC
CGTGTGTTTGATATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTTGTA
CTGGATGAAGCTGACGAAATGTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTC
CAAAAGCTCAACAGCAACACCCAGGTAGTTTTGCTGTCAGCCACAATGCCTTCTGATGTG
CTTGAGGTGACCAAGAAGTTCATGAGGGACCCCATTCGGATTCTTGTCAAGAAGGAAGAG
TTGACCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGGAAGCTG
GACACACTATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATCTTCATCAAC
ACCCGGAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCTCGAGATTTCACTGTATCC
GCCATGCATGGAGATATGGACCAAAAGGAACGAGACGTGATTATGAGGGAGTTTCGTTCT
GGCTCTAGCAGAGTTTTGATTACCACTGACCTGCTGGCCAGAGGCATTGATGTGCAGCAG
GTTTCTTTAGTCATCAACTATGACCTTCCCACCAACAGGGAAAACTATATCCACAGAATC
GGTCGAGGTGGACGGTTTGGCCGTAAAGGTGTGGCTATTAACATGGTGACAGAAGAAGAC
AAGAGGACTCTTCGAGACATTGAGACCTTCTACAACACCTCCATTGAGGAAATGCCCCTC
AATGTTGCTGACCTCATC

ORF Protein

Cloned ORF protein sequence for pF1KB3435 Download

>KIBB3435  406 aa
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQ
RAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVV
MALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFV
LDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEE
LTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVS
AMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRI
GRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB3435 Download

>pF1KB3435  4331 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGTCTGCGAGC
CAGGATTCCCGATCCAGAGACAATGGCCCCGATGGGATGGAGCCCGAAGGCGTCATCGAG
AGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCGGAGTCCCTTCTC
CGTGGCATCTACGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCCATTCTA
CCTTGTATCAAGGGTTATGATGTGATTGCTCAAGCCCAATCTGGGACTGGGAAAACGGCC
ACATTTGCCATATCGATTCTGCAGCAGATTGAATTAGATCTAAAAGCCACCCAGGCCTTG
GTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACAGAAGGTGGTCATGGCACTAGGA
GACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTGCGTGCTGAGGTG
CAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGCCGTGTGTTTGAT
ATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTTGTACTGGATGAAGCT
GACGAAATGTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTCCAAAAGCTCAAC
AGCAACACCCAGGTAGTTTTGCTGTCAGCCACAATGCCTTCTGATGTGCTTGAGGTGACC
AAGAAGTTCATGAGGGACCCCATTCGGATTCTTGTCAAGAAGGAAGAGTTGACCCTGGAG
GGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGGAAGCTGGACACACTATGT
GACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATCTTCATCAACACCCGGAGGAAG
GTGGACTGGCTCACCGAGAAGATGCATGCTCGAGATTTCACTGTATCCGCCATGCATGGA
GATATGGACCAAAAGGAACGAGACGTGATTATGAGGGAGTTTCGTTCTGGCTCTAGCAGA
GTTTTGATTACCACTGACCTGCTGGCCAGAGGCATTGATGTGCAGCAGGTTTCTTTAGTC
ATCAACTATGACCTTCCCACCAACAGGGAAAACTATATCCACAGAATCGGTCGAGGTGGA
CGGTTTGGCCGTAAAGGTGTGGCTATTAACATGGTGACAGAAGAAGACAAGAGGACTCTT
CGAGACATTGAGACCTTCTACAACACCTCCATTGAGGAAATGCCCCTCAATGTTGCTGAC
CTCATCGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGG
CATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCAC
CGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTT
GCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTA
TCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGT
CCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTT
CTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCT
TCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAAC
TGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAG
ACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCC
GCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGAT
GCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTG
TCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACG
GGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA
TTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTA
TCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTC
GACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTC
GATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGG
CTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTG
CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT
GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGC
GGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGC
ATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGA
CCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC
GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA
GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA
CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG
TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG
TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG
AAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT
CCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAA
ACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGG
TTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTG
GTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGC
GTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAA
ACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGAT
ACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAG
CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGG
TAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGG
CTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA
GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGC
GGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGG
ATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCAT