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SpecieshumanProduct IDFXC02010Cloning SiteSgfI-PmeISymbolMYH15Descriptionmyosin, heavy chain 15NoteThis plasmid tends to take in E.coli genomic DNA by recombination. The E.coli strain stbl4 seems free from this recombination.
Length: 5838 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1946 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS43127.1 0 99.9 MYH15 CCDS9600.1 1.4e-173 62.5 MYH6 CCDS9601.1 9.1e-173 62.1 MYH7 CCDS11153.1 5.6e-166 59.5 MYH8 CCDS11157.1 4e-165 60.1 MYH3
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR001609 133 152 PR00193 Myosin head IPR001609 189 214 PR00193 Myosin head IPR001609 238 265 PR00193 Myosin head IPR001609 468 496 PR00193 Myosin head IPR001609 522 550 PR00193 Myosin head HMMPfam IPR004009 51 92 PF02736 Myosin IPR001609 106 778 PF00063 Myosin head IPR002928 1081 1937 PF01576 Myosin tail HMMSmart IPR001609 97 791 SM00242 Myosin head
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055796 1946 609929 99.9 100.0 XP_011510861 1946 609929 99.9 100.0 XP_016861411 1599 609929 99.9 82.2 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pF1KA1000 Download>KIAA1000 5838 bp ATGGTTGAATCTTGCCTACTCACGTTTAGGGCCTTTTTTTGGTGGATTGCTTTAATAAAG ATGGATCTGTCAGACCTTGGAGAAGCCGCAGCCTTCCTCAGAAGAAGTGAAGCTGAGCTG CTTCTACTACAGGCCACAGCCTTGGATGGGAAGAAGAAATGCTGGATTCCTGATGGTGAG AATGCTTATATCGAGGCTGAGGTAAAAGGGAGTGAAGATGATGGAACAGTAATTGTTGAG ACAGCAGATGGAGAGAGTCTGAGCATAAAGGAGGACAAAATCCAGCAGATGAATCCTCCA GAGTTTGAAATGATTGAAGACATGGCAATGCTGACTCACCTCAATGAGGCATCCGTGCTG CATACCCTGAAGCGGCGCTATGGCCAGTGGATGATCTATACATATTCAGGTCTCTTCTGT GTGACCATAAACCCTTACAAATGGCTTCCCGTGTATCAGAAAGAAGTCATGGCCGCCTAC AAAGGGAAGAGGCGATCAGAGGCTCCCCCTCACATCTTTGCTGTTGCCAATAACGCCTTT CAGGACATGCTTCACAATCGAGAAAATCAGTCTATACTCTTCACAGGAGAATCTGGTGCT GGAAAGACTGTGAACAGCAAACATATTATCCAGTATTTTGCCACCATAGCAGCCATGATT GAATCCAGGAAAAAGCAGGGGGCGTTAGAAGATCAAATCATGCAAGCGAATACTATCTTG GAAGCATTTGGAAATGCTAAAACCCTGAGAAATGACAACTCCTCTCGTTTTGGCAAATTC ATCAGGATGCACTTTGGTGCCAGAGGCATGCTGTCATCTGTGGACATTGATATCTATTTG CTTGAAAAGTCCAGGGTGATTTTCCAGCAGGCTGGAGAGAGGAACTACCACATATTCTAT CAAATTCTATCTGGACAAAAAGAGCTTCATGACCTGCTCCTGGTATCTGCAAATCCCTCA GACTTCCACTTTTGCTCCTGTGGAGCAGTTACTGTGGAGAGCTTGGATGATGCTGAAGAA TTGCTGGCCACAGAACAAGCCATGGACATCTTGGGCTTTCTTCCTGATGAGAAGTATGGA TGCTATAAACTCACTGGAGCCATCATGCACTTTGGAAATATGAAATTTAAACAGAAACCT AGAGAAGAGCAACTGGAAGCAGATGGCACAGAAAATGCTGACAAAGCTGCTTTCCTCATG GGCATTAACTCCTCTGAGTTGGTAAAGTGCTTGATCCATCCTAGAATCAAAGTTGGTAAC GAATATGTTACCAGAGGTCAAACTATAGAACAGGTAACCTGTGCTGTCGGTGCCCTGTCC AAGTCAATGTATGAAAGGATGTTTAAGTGGCTAGTGGCACGGATCAACAGGGCCCTGGAT GCCAAGCTGTCAAGGCAGTTCTTCATTGGCATTCTTGACATCACTGGTTTTGAAATCCTT GAGTATAATAGCCTTGAGCAACTTTGCATTAATTTTACCAATGAAAAATTACAACAATTC TTCAATTGGTACATGTTTGTTCTGGAGCAAGAGGAATATAAGAAAGAAAGCATTGAATGG GTGTCTATTGGCTTTGGTCTGGATTTGCAAGCTTGCATAGATCTCATTGAGAAGCCAATG GGCATCCTTTCCATCCTTGAAGAAGAGTGTATGTTTCCTAAGGCTACAGACCTGACTTTC AAGACCAAACTCTTTGACAACCATTTTGGAAAGTCGGTTCATCTCCAGAAGCCCAAGCCT GATAAGAAGAAATTTGAAGCTCATTTTGAACTTGTCCATTATGCAGGAGTGGTACCTTAT AATATCAGTGGTTGGCTGGAAAAGAACAAAGACCTCCTTAATGAAACAGTGGTAGCTGTA TTTCAGAAGTCTTCCAACAGACTCCTGGCGAGCCTTTTTGAAAATTACATGAGTACTGAC AGTGCTATACCATTTGGGGAGAAGAAACGAAAGAAAGGAGCTTCATTCCAAACGGTTGCA TCTCTGCATAAAGAAAACCTGAATAAATTGATGACTAATCTGAAATCAACAGCACCTCAT TTTGTGAGATGCATAAATCCCAATGTGAACAAAATACCAGGTATACTGGACCCTTACTTG GTTCTACAGCAGTTGCGCTGTAATGGTGTCTTGGAAGGGACTAGGATATGCCGTGAAGGT TTTCCAAACCGACTGCAGTATGCTGATTTTAAACAAAGGTACTGCATTCTGAATCCAAGG ACCTTTCCAAAGAGCAAGTTTGTGAGCAGCAGAAAAGCAGCTGAAGAATTACTTGGCTCC TTGGAGATAGACCATACCCAGTACCGATTTGGAATCACTAAGGTGTTTTTTAAAGCTGGG TTTCTGGGCCAACTGGAAGCAATAAGAGATGAGAGACTATCTAAAGTCTTCACATTGTTC CAAGCCAGAGCACAGGGCAAACTGATGCGAATCAAATTCCAGAAGATTCTGGAAGAAAGG GATGCACTTATTTTGATCCAATGGAACATAAGAGCTTTCATGGCTGTGAAGAACTGGCCC TGGATGAGGCTCTTCTTCAAGATCAAGCCTCTTGTTAAATCTTCAGAAGTAGGAGAAGAA GTAGCTGGACTGAAGGAAGAGTGTGCACAATTACAGAAAGCCTTGGAGAAATCAGAGTTT CAGAGGGAGGAACTGAAAGCAAAGCAAGTATCCCTCACTCAGGAAAAGAATGACCTGATT CTTCAGCTTCAGGCTGAGCAAGAGACACTGGCAAATGTTGAAGAGCAGTGCGAGTGGCTG ATTAAATCCAAGATCCAGCTGGAGGCCAGAGTAAAGGAGCTGTCGGAGAGGGTGGAGGAA GAAGAGGAGATAAATTCTGAGCTGACTGCCAGGGGGCGGAAACTCGAAGATGAATGTTTT GAGTTGAAGAAAGAAATCGATGACCTGGAAACAATGTTGGTGAAGTCAGAGAAGGAGAAG CGTACTACAGAGCACAAGGTCAAGAACTTGACTGAGGAAGTAGAGTTTCTAAATGAGGAT ATCAGCAAACTTAACAGAGCAGCCAAGGTTGTGCAGGAGGCCCATCAGCAGACCCTGGAT GACCTGCACATGGAGGAGGAGAAGCTCAGCAGCCTGAGCAAAGCAAATCTGAAGCTGGAA CAGCAAGTTGATGAGCTTGAGGGTGCCCTTGAGCAGGAGAGAAAAGCGAGAATGAACTGT GAAAGGGAACTGCACAAACTGGAGGGCAATTTAAAGCTGAATCGGGAAAGTATGGAGAAC CTGGAAAGCAGCCAGCGACACCTGGCAGAAGAGCTGAGGAAAAAAGAATTAGAATTGAGT CAGATGAATTCAAAAGTGGAGAATGAGAAAGGCCTGGTAGCTCAGCTTCAGAAGACGGTT AAAGAGCTTCAGACTCAAATAAAGGATTTGAAAGAGAAACTAGAAGCTGAAAGGACCACT CGAGCCAAGATGGAAAGGGAGAGAGCTGACCTCACCCAAGACCTGGCTGACTTGAATGAG AGGCTGGAGGAGGTAGGAGGATCCAGTTTGGCTCAGCTGGAAATAACTAAGAAACAGGAA ACCAAATTCCAGAAGCTGCACCGAGACATGGAAGAGGCCACTCTGCACTTTGAGACAACT TCTGCATCTTTGAAGAAGAGACATGCAGACAGCCTGGCTGAGCTCGAGGGCCAGGTAGAA AATCTACAGCAGGTCAAGCAGAAACTGGAAAAAGACAAGAGTGACTTGCAGCTAGAAGTA GATGACCTCCTGACCCGTGTTGAGCAGATGACAAGAGCTAAGGCAAATGCTGAGAAACTC TGTACTCTATATGAAGAGCGCTTGCATGAAGCAACTGCAAAGCTAGATAAGGTGACTCAG TTGGCAAATGACCTGGCAGCACAAAAGACAAAGCTGTGGAGTGAGAGTGGCGAGTTCCTA CGGAGGCTTGAAGAGAAGGAGGCTCTGATAAACCAACTTTCCAGGGAAAAGAGCAACTTC ACTCGGCAGATTGAAGACCTGAGAGGGCAGCTGGAAAAGGAGACCAAATCCCAGAGTGCC CTGGCCCATGCCCTGCAGAAGGCTCAGCGTGACTGTGACCTTCTACGAGAGCAGTATGAG GAAGAACAAGAGGTCAAGGCTGAGCTGCACCGGACCTTATCCAAAGTCAATGCTGAAATG GTGCAATGGAGAATGAAGTATGAAAACAATGTCATCCAGAGAACAGAAGACTTGGAGGAT GCCAAGAAGGAACTGGCAATTAGATTGCAGGAGGCAGCCGAAGCCATGGGGGTGGCCAAT GCCAGAAATGCCTCCTTGGAGAGAGCCAGGCACCAGCTGCAGCTGGAGCTCGGGGACGCC CTGTCTGACCTCGGGAAGGTCCGCTCTGCAGCAGCCAGGCTGGACCAGAAGCAGCTGCAG TCTGGCAAGGCCCTTGCCGACTGGAAGCAGAAGCACGAGGAGTCCCAGGCGTTGCTGGAT GCCTCTCAGAAGGAAGTTCAGGCTCTCAGTACAGAGCTCCTCAAGCTCAAGAACACCTAT GAGGAGAGCATCGTGGGCCAGGAGACACTCAGGAGGGAGAACAAGAACCTCCAAGAAGAG ATTTCTAATCTGACAAACCAGGTTAGAGAAGGGACCAAGAACTTAACTGAAATGGAAAAG GTCAAGAAACTAATTGAAGAAGAGAAGACAGAAGTCCAGGTGACACTGGAAGAAACAGAG GGAGCCCTGGAACGTAATGAAAGCAAGATTCTTCATTTCCAGCTTGAACTCTTGGAAGCT AAAGCAGAACTTGAAAGAAAGCTTTCAGAGAAAGATGAAGAAATAGAAAATTTTAGGAGG AAGCAGCAGTGTACCATTGACTCCCTGCAGTCTAGTCTGGATTCTGAAGCTAAGAGCAGA ATTGAGGTTACCCGGCTGAAGAAGAAGATGGAAGAGGACCTCAATGAGATGGAACTCCAG CTTAGCTGTGCCAACCGGCAGGTGTCAGAAGCAACCAAATCCCTGGGCCAGCTTCAGATT CAAATCAAGGACCTTCAAATGCAGCTGGATGACAGCACACAACTGAACAGTGATCTGAAG GAGCAGGTGGCTGTGGCTGAGCGGCGCAACTCTCTTCTTCAGTCTGAACTAGAGGATCTA AGGTCCCTGCAAGAGCAGACAGAGCGTGGCCGCAGGCTGTCAGAAGAAGAGCTCCTGGAA GCAACAGAAAGAATCAATCTTTTCTATACCCAGAACACAAGCCTCCTCAGCCAGAAGAAG AAACTGGAGGCTGATGTTGCCCGGATGCAGAAAGAAGCTGAAGAGGTGGTGCAGGAGTGT CAAAATGCAGAAGAGAAGGCCAAGAAGGCAGCCATTGAGGCAGCAAACTTGTCAGAAGAA CTGAAGAAGAAGCAAGACACCATTGCCCACTTGGAAAGGACAAGAGAAAATATGGAGCAG ACAATTACAGACTTACAGAAAAGGCTGGCTGAAGCTGAACAGATGGCCCTGATGGGGAGT AGAAAGCAAATCCAGAAACTAGAATCCAGGGTTCGTGAACTGGAAGGTGAACTGGAGGGT GAAATCCGTCGCAGTGCAGAGGCCCAGAGGGGAGCCCGCAGACTTGAGCGATGCATCAAA GAGCTGACCTATCAGGCAGAGGAAGACAAGAAGAATCTGAGCAGGATGCAAACTCAGATG GATAAACTTCAGCTAAAAGTGCAAAATTACAAGCAGCAAGTCGAGGTGGCGGAAACACAA GCCAATCAATACCTTTCCAAGTATAAGAAACAGCAACATGAGTTGAATGAAGTGAAGGAA AGGGCAGAGGTGGCAGAATCTCAAGTCAATAAACTCAAAATTAAAGCAAGAGAGTTTGGG AAAAAGGTTCAAGAAGAA
Cloned ORF protein sequence for pF1KA1000 Download>KIAA1000 1946 aa MVESCLLTFRAFFWWIALIKMDLSDLGEAAAFLRRSEAELLLLQATALDGKKKCWIPDGE NAYIEAEVKGSEDDGTVIVETADGESLSIKEDKIQQMNPPEFEMIEDMAMLTHLNEASVL HTLKRRYGQWMIYTYSGLFCVTINPYKWLPVYQKEVMAAYKGKRRSEAPPHIFAVANNAF QDMLHNRENQSILFTGESGAGKTVNSKHIIQYFATIAAMIESRKKQGALEDQIMQANTIL EAFGNAKTLRNDNSSRFGKFIRMHFGARGMLSSVDIDIYLLEKSRVIFQQAGERNYHIFY QILSGQKELHDLLLVSANPSDFHFCSCGAVTVESLDDAEELLATEQAMDILGFLPDEKYG CYKLTGAIMHFGNMKFKQKPREEQLEADGTENADKAAFLMGINSSELVKCLIHPRIKVGN EYVTRGQTIEQVTCAVGALSKSMYERMFKWLVARINRALDAKLSRQFFIGILDITGFEIL EYNSLEQLCINFTNEKLQQFFNWYMFVLEQEEYKKESIEWVSIGFGLDLQACIDLIEKPM GILSILEEECMFPKATDLTFKTKLFDNHFGKSVHLQKPKPDKKKFEAHFELVHYAGVVPY NISGWLEKNKDLLNETVVAVFQKSSNRLLASLFENYMSTDSAIPFGEKKRKKGASFQTVA SLHKENLNKLMTNLKSTAPHFVRCINPNVNKIPGILDPYLVLQQLRCNGVLEGTRICREG FPNRLQYADFKQRYCILNPRTFPKSKFVSSRKAAEELLGSLEIDHTQYRFGITKVFFKAG FLGQLEAIRDERLSKVFTLFQARAQGKLMRIKFQKILEERDALILIQWNIRAFMAVKNWP WMRLFFKIKPLVKSSEVGEEVAGLKEECAQLQKALEKSEFQREELKAKQVSLTQEKNDLI LQLQAEQETLANVEEQCEWLIKSKIQLEARVKELSERVEEEEEINSELTARGRKLEDECF ELKKEIDDLETMLVKSEKEKRTTEHKVKNLTEEVEFLNEDISKLNRAAKVVQEAHQQTLD DLHMEEEKLSSLSKANLKLEQQVDELEGALEQERKARMNCERELHKLEGNLKLNRESMEN LESSQRHLAEELRKKELELSQMNSKVENEKGLVAQLQKTVKELQTQIKDLKEKLEAERTT RAKMERERADLTQDLADLNERLEEVGGSSLAQLEITKKQETKFQKLHRDMEEATLHFETT SASLKKRHADSLAELEGQVENLQQVKQKLEKDKSDLQLEVDDLLTRVEQMTRAKANAEKL CTLYEERLHEATAKLDKVTQLANDLAAQKTKLWSESGEFLRRLEEKEALINQLSREKSNF TRQIEDLRGQLEKETKSQSALAHALQKAQRDCDLLREQYEEEQEVKAELHRTLSKVNAEM VQWRMKYENNVIQRTEDLEDAKKELAIRLQEAAEAMGVANARNASLERARHQLQLELGDA LSDLGKVRSAAARLDQKQLQSGKALADWKQKHEESQALLDASQKEVQALSTELLKLKNTY EESIVGQETLRRENKNLQEEISNLTNQVREGTKNLTEMEKVKKLIEEEKTEVQVTLEETE GALERNESKILHFQLELLEAKAELERKLSEKDEEIENFRRKQQCTIDSLQSSLDSEAKSR IEVTRLKKKMEEDLNEMELQLSCANRQVSEATKSLGQLQIQIKDLQMQLDDSTQLNSDLK EQVAVAERRNSLLQSELEDLRSLQEQTERGRRLSEEELLEATERINLFYTQNTSLLSQKK KLEADVARMQKEAEEVVQECQNAEEKAKKAAIEAANLSEELKKKQDTIAHLERTRENMEQ TITDLQKRLAEAEQMALMGSRKQIQKLESRVRELEGELEGEIRRSAEAQRGARRLERCIK ELTYQAEEDKKNLSRMQTQMDKLQLKVQNYKQQVEVAETQANQYLSKYKKQQHELNEVKE RAEVAESQVNKLKIKAREFGKKVQEE
Nucleotide Sequence (with vector) for pF1KA1000 Download>pF1KA1000 8951 bp GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGTTGAATCT TGCCTACTCACGTTTAGGGCCTTTTTTTGGTGGATTGCTTTAATAAAGATGGATCTGTCA GACCTTGGAGAAGCCGCAGCCTTCCTCAGAAGAAGTGAAGCTGAGCTGCTTCTACTACAG GCCACAGCCTTGGATGGGAAGAAGAAATGCTGGATTCCTGATGGTGAGAATGCTTATATC GAGGCTGAGGTAAAAGGGAGTGAAGATGATGGAACAGTAATTGTTGAGACAGCAGATGGA GAGAGTCTGAGCATAAAGGAGGACAAAATCCAGCAGATGAATCCTCCAGAGTTTGAAATG ATTGAAGACATGGCAATGCTGACTCACCTCAATGAGGCATCCGTGCTGCATACCCTGAAG CGGCGCTATGGCCAGTGGATGATCTATACATATTCAGGTCTCTTCTGTGTGACCATAAAC CCTTACAAATGGCTTCCCGTGTATCAGAAAGAAGTCATGGCCGCCTACAAAGGGAAGAGG CGATCAGAGGCTCCCCCTCACATCTTTGCTGTTGCCAATAACGCCTTTCAGGACATGCTT CACAATCGAGAAAATCAGTCTATACTCTTCACAGGAGAATCTGGTGCTGGAAAGACTGTG AACAGCAAACATATTATCCAGTATTTTGCCACCATAGCAGCCATGATTGAATCCAGGAAA AAGCAGGGGGCGTTAGAAGATCAAATCATGCAAGCGAATACTATCTTGGAAGCATTTGGA AATGCTAAAACCCTGAGAAATGACAACTCCTCTCGTTTTGGCAAATTCATCAGGATGCAC TTTGGTGCCAGAGGCATGCTGTCATCTGTGGACATTGATATCTATTTGCTTGAAAAGTCC AGGGTGATTTTCCAGCAGGCTGGAGAGAGGAACTACCACATATTCTATCAAATTCTATCT GGACAAAAAGAGCTTCATGACCTGCTCCTGGTATCTGCAAATCCCTCAGACTTCCACTTT TGCTCCTGTGGAGCAGTTACTGTGGAGAGCTTGGATGATGCTGAAGAATTGCTGGCCACA GAACAAGCCATGGACATCTTGGGCTTTCTTCCTGATGAGAAGTATGGATGCTATAAACTC ACTGGAGCCATCATGCACTTTGGAAATATGAAATTTAAACAGAAACCTAGAGAAGAGCAA CTGGAAGCAGATGGCACAGAAAATGCTGACAAAGCTGCTTTCCTCATGGGCATTAACTCC TCTGAGTTGGTAAAGTGCTTGATCCATCCTAGAATCAAAGTTGGTAACGAATATGTTACC AGAGGTCAAACTATAGAACAGGTAACCTGTGCTGTCGGTGCCCTGTCCAAGTCAATGTAT GAAAGGATGTTTAAGTGGCTAGTGGCACGGATCAACAGGGCCCTGGATGCCAAGCTGTCA AGGCAGTTCTTCATTGGCATTCTTGACATCACTGGTTTTGAAATCCTTGAGTATAATAGC CTTGAGCAACTTTGCATTAATTTTACCAATGAAAAATTACAACAATTCTTCAATTGGTAC ATGTTTGTTCTGGAGCAAGAGGAATATAAGAAAGAAAGCATTGAATGGGTGTCTATTGGC TTTGGTCTGGATTTGCAAGCTTGCATAGATCTCATTGAGAAGCCAATGGGCATCCTTTCC ATCCTTGAAGAAGAGTGTATGTTTCCTAAGGCTACAGACCTGACTTTCAAGACCAAACTC TTTGACAACCATTTTGGAAAGTCGGTTCATCTCCAGAAGCCCAAGCCTGATAAGAAGAAA TTTGAAGCTCATTTTGAACTTGTCCATTATGCAGGAGTGGTACCTTATAATATCAGTGGT TGGCTGGAAAAGAACAAAGACCTCCTTAATGAAACAGTGGTAGCTGTATTTCAGAAGTCT TCCAACAGACTCCTGGCGAGCCTTTTTGAAAATTACATGAGTACTGACAGTGCTATACCA TTTGGGGAGAAGAAACGAAAGAAAGGAGCTTCATTCCAAACGGTTGCATCTCTGCATAAA GAAAACCTGAATAAATTGATGACTAATCTGAAATCAACAGCACCTCATTTTGTGAGATGC ATAAATCCCAATGTGAACAAAATACCAGGTATACTGGACCCTTACTTGGTTCTACAGCAG TTGCGCTGTAATGGTGTCTTGGAAGGGACTAGGATATGCCGTGAAGGTTTTCCAAACCGA CTGCAGTATGCTGATTTTAAACAAAGGTACTGCATTCTGAATCCAAGGACCTTTCCAAAG AGCAAGTTTGTGAGCAGCAGAAAAGCAGCTGAAGAATTACTTGGCTCCTTGGAGATAGAC CATACCCAGTACCGATTTGGAATCACTAAGGTGTTTTTTAAAGCTGGGTTTCTGGGCCAA CTGGAAGCAATAAGAGATGAGAGACTATCTAAAGTCTTCACATTGTTCCAAGCCAGAGCA CAGGGCAAACTGATGCGAATCAAATTCCAGAAGATTCTGGAAGAAAGGGATGCACTTATT TTGATCCAATGGAACATAAGAGCTTTCATGGCTGTGAAGAACTGGCCCTGGATGAGGCTC TTCTTCAAGATCAAGCCTCTTGTTAAATCTTCAGAAGTAGGAGAAGAAGTAGCTGGACTG AAGGAAGAGTGTGCACAATTACAGAAAGCCTTGGAGAAATCAGAGTTTCAGAGGGAGGAA CTGAAAGCAAAGCAAGTATCCCTCACTCAGGAAAAGAATGACCTGATTCTTCAGCTTCAG GCTGAGCAAGAGACACTGGCAAATGTTGAAGAGCAGTGCGAGTGGCTGATTAAATCCAAG ATCCAGCTGGAGGCCAGAGTAAAGGAGCTGTCGGAGAGGGTGGAGGAAGAAGAGGAGATA AATTCTGAGCTGACTGCCAGGGGGCGGAAACTCGAAGATGAATGTTTTGAGTTGAAGAAA GAAATCGATGACCTGGAAACAATGTTGGTGAAGTCAGAGAAGGAGAAGCGTACTACAGAG CACAAGGTCAAGAACTTGACTGAGGAAGTAGAGTTTCTAAATGAGGATATCAGCAAACTT AACAGAGCAGCCAAGGTTGTGCAGGAGGCCCATCAGCAGACCCTGGATGACCTGCACATG GAGGAGGAGAAGCTCAGCAGCCTGAGCAAAGCAAATCTGAAGCTGGAACAGCAAGTTGAT GAGCTTGAGGGTGCCCTTGAGCAGGAGAGAAAAGCGAGAATGAACTGTGAAAGGGAACTG CACAAACTGGAGGGCAATTTAAAGCTGAATCGGGAAAGTATGGAGAACCTGGAAAGCAGC CAGCGACACCTGGCAGAAGAGCTGAGGAAAAAAGAATTAGAATTGAGTCAGATGAATTCA AAAGTGGAGAATGAGAAAGGCCTGGTAGCTCAGCTTCAGAAGACGGTTAAAGAGCTTCAG ACTCAAATAAAGGATTTGAAAGAGAAACTAGAAGCTGAAAGGACCACTCGAGCCAAGATG GAAAGGGAGAGAGCTGACCTCACCCAAGACCTGGCTGACTTGAATGAGAGGCTGGAGGAG GTAGGAGGATCCAGTTTGGCTCAGCTGGAAATAACTAAGAAACAGGAAACCAAATTCCAG AAGCTGCACCGAGACATGGAAGAGGCCACTCTGCACTTTGAGACAACTTCTGCATCTTTG AAGAAGAGACATGCAGACAGCCTGGCTGAGCTCGAGGGCCAGGTAGAAAATCTACAGCAG GTCAAGCAGAAACTGGAAAAAGACAAGAGTGACTTGCAGCTAGAAGTAGATGACCTCCTG ACCCGTGTTGAGCAGATGACAAGAGCTAAGGCAAATGCTGAGAAACTCTGTACTCTATAT GAAGAGCGCTTGCATGAAGCAACTGCAAAGCTAGATAAGGTGACTCAGTTGGCAAATGAC CTGGCAGCACAAAAGACAAAGCTGTGGAGTGAGAGTGGCGAGTTCCTACGGAGGCTTGAA GAGAAGGAGGCTCTGATAAACCAACTTTCCAGGGAAAAGAGCAACTTCACTCGGCAGATT GAAGACCTGAGAGGGCAGCTGGAAAAGGAGACCAAATCCCAGAGTGCCCTGGCCCATGCC CTGCAGAAGGCTCAGCGTGACTGTGACCTTCTACGAGAGCAGTATGAGGAAGAACAAGAG GTCAAGGCTGAGCTGCACCGGACCTTATCCAAAGTCAATGCTGAAATGGTGCAATGGAGA ATGAAGTATGAAAACAATGTCATCCAGAGAACAGAAGACTTGGAGGATGCCAAGAAGGAA CTGGCAATTAGATTGCAGGAGGCAGCCGAAGCCATGGGGGTGGCCAATGCCAGAAATGCC TCCTTGGAGAGAGCCAGGCACCAGCTGCAGCTGGAGCTCGGGGACGCCCTGTCTGACCTC GGGAAGGTCCGCTCTGCAGCAGCCAGGCTGGACCAGAAGCAGCTGCAGTCTGGCAAGGCC CTTGCCGACTGGAAGCAGAAGCACGAGGAGTCCCAGGCGTTGCTGGATGCCTCTCAGAAG GAAGTTCAGGCTCTCAGTACAGAGCTCCTCAAGCTCAAGAACACCTATGAGGAGAGCATC GTGGGCCAGGAGACACTCAGGAGGGAGAACAAGAACCTCCAAGAAGAGATTTCTAATCTG ACAAACCAGGTTAGAGAAGGGACCAAGAACTTAACTGAAATGGAAAAGGTCAAGAAACTA ATTGAAGAAGAGAAGACAGAAGTCCAGGTGACACTGGAAGAAACAGAGGGAGCCCTGGAA CGTAATGAAAGCAAGATTCTTCATTTCCAGCTTGAACTCTTGGAAGCTAAAGCAGAACTT GAAAGAAAGCTTTCAGAGAAAGATGAAGAAATAGAAAATTTTAGGAGGAAGCAGCAGTGT ACCATTGACTCCCTGCAGTCTAGTCTGGATTCTGAAGCTAAGAGCAGAATTGAGGTTACC CGGCTGAAGAAGAAGATGGAAGAGGACCTCAATGAGATGGAACTCCAGCTTAGCTGTGCC AACCGGCAGGTGTCAGAAGCAACCAAATCCCTGGGCCAGCTTCAGATTCAAATCAAGGAC CTTCAAATGCAGCTGGATGACAGCACACAACTGAACAGTGATCTGAAGGAGCAGGTGGCT GTGGCTGAGCGGCGCAACTCTCTTCTTCAGTCTGAACTAGAGGATCTAAGGTCCCTGCAA GAGCAGACAGAGCGTGGCCGCAGGCTGTCAGAAGAAGAGCTCCTGGAAGCAACAGAAAGA ATCAATCTTTTCTATACCCAGAACACAAGCCTCCTCAGCCAGAAGAAGAAACTGGAGGCT GATGTTGCCCGGATGCAGAAAGAAGCTGAAGAGGTGGTGCAGGAGTGTCAAAATGCAGAA GAGAAGGCCAAGAAGGCAGCCATTGAGGCAGCAAACTTGTCAGAAGAACTGAAGAAGAAG CAAGACACCATTGCCCACTTGGAAAGGACAAGAGAAAATATGGAGCAGACAATTACAGAC TTACAGAAAAGGCTGGCTGAAGCTGAACAGATGGCCCTGATGGGGAGTAGAAAGCAAATC CAGAAACTAGAATCCAGGGTTCGTGAACTGGAAGGTGAACTGGAGGGTGAAATCCGTCGC AGTGCAGAGGCCCAGAGGGGAGCCCGCAGACTTGAGCGATGCATCAAAGAGCTGACCTAT CAGGCAGAGGAAGACAAGAAGAATCTGAGCAGGATGCAAACTCAGATGGATAAACTTCAG CTAAAAGTGCAAAATTACAAGCAGCAAGTCGAGGTGGCGGAAACACAAGCCAATCAATAC CTTTCCAAGTATAAGAAACAGCAACATGAGTTGAATGAAGTGAAGGAAAGGGCAGAGGTG GCAGAATCTCAAGTCAATAAACTCAAAATTAAAGCAAGAGAGTTTGGGAAAAAGGTTCAA GAAGAAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGG CATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCAC CGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTT GCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTA TCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGT CCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTT CTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCT TCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAAC TGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAG ACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCC GCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGAT GCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTG TCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACG GGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA TTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTA TCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTC GACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTC GATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGG CTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTG CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGC GGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGC ATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGA CCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG AAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT CCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAA ACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGG TTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTG GTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGC GTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAA ACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGAT ACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAG CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGG TAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGG CTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGC GGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGG ATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATG TATCCGCTCAT
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