ERAS ORF clone FXC29433

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Flexi ORF Clone FXC29433

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Clone info.

Species

human

Clone Name

pF1KE1722 Sequence (with vector) Restriction Map

Product ID

FXC29433

HaloTag Clone PID

FHC29433

ORF Sequence

DNA sequence (699 bp)
Protein sequence (233 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1722

Symbol

ERAS

Alias : HRAS2, HRASP

Description

ES cell expressed Ras

Original Clone ID

cs02622

Features of the cloned ORF sequence

Description

Length: 699 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 233 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35246.1 4.5e-92 100.0 ERAS CCDS8702.1 1e-25 47.3 KRAS CCDS877.1 1.3e-24 47.5 NRAS CCDS8703.1 6.2e-24 47.3 KRAS CCDS7698.1 1.7e-23 48.8 HRAS The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 42 63 PR00449 Ras GTPase IPR001806 65 81 PR00449 Ras GTPase IPR001806 82 104 PR00449 Ras GTPase IPR001806 142 155 PR00449 Ras GTPase IPR001806 177 199 PR00449 Ras GTPase HMMPfam IPR013753 43 200 PF00071 Ras HMMSmart IPR003577 39 202 SM00173 Ras small GTPase IPR003579 42 202 SM00175 Ras small GTPase IPR003578 44 202 SM00174 Small GTPase HMMTigr IPR005225 42 195 TIGR00231 Small GTP-binding protein domain ProfileScan IPR020849 37 233 PS51421 Ras small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_853510 233 300437 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE1722 Download

>KIEE1722  699 bp
ATGGAGCTGCCAACAAAGCCTGGCACCTTCGACCTGGGCCTGGCCACATGGAGCCCTTCC
TTCCAGGGGGAAACCCACCGGGCTCAGGCACGCCGCAGGGATGTTGGCAGGCAGCTGCCT
GAGTACAAGGCTGTGGTGGTGGGCGCCAGTGGCGTGGGCAAGAGTGCGCTGACCATCCAG
CTGAACCACCAGTGCTTCGTGGAGGACCACGACCCCACCATCCAGGATTCCTACTGGAAG
GAGTTGACCCTGGACAGTGGGGACTGCATTCTGAATGTGCTGGACACAGCAGGGCAGGCC
ATCCATAGGGCCCTGCGTGACCAGTGCCTGGCTGTCTGTGATGGTGTGCTGGGCGTCTTC
GCTCTCGATGACCCCTCGTCTCTGATCCAGCTGCAGCAGATATGGGCCACCTGGGGCCCT
CACCCCGCCCAGCCCCTTGTCCTCGTGGGCAACAAGTGTGACCTTGTGACCACTGCTGGA
GATGCTCATGCCGCTGCTGCAGCCCTCGCACACAGCTGGGGGGCCCACTTCGTGGAGACC
TCGGCCAAAACACGGCAAGGCGTGGAGGAGGCCTTTTCCCTGCTGGTCCATGAGATCCAG
AGGGTCCAGGAGGCCATGGCGAAGGAGCCCATGGCAAGGTCCTGTAGGGAGAAGACCCGG
CACCAGAAGGCCACCTGCCACTGTGGCTGCTCTGTGGCC

ORF Protein

Cloned ORF protein sequence for pF1KE1722 Download

>KIEE1722  233 aa
MELPTKPGTFDLGLATWSPSFQGETHRAQARRRDVGRQLPEYKAVVVGASGVGKSALTIQ
LNHQCFVEDHDPTIQDSYWKELTLDSGDCILNVLDTAGQAIHRALRDQCLAVCDGVLGVF
ALDDPSSLIQLQQIWATWGPHPAQPLVLVGNKCDLVTTAGDAHAAAAALAHSWGAHFVET
SAKTRQGVEEAFSLLVHEIQRVQEAMAKEPMARSCREKTRHQKATCHCGCSVA

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE1722 Download

>pF1KE1722  3812 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGAGCTGCCA
ACAAAGCCTGGCACCTTCGACCTGGGCCTGGCCACATGGAGCCCTTCCTTCCAGGGGGAA
ACCCACCGGGCTCAGGCACGCCGCAGGGATGTTGGCAGGCAGCTGCCTGAGTACAAGGCT
GTGGTGGTGGGCGCCAGTGGCGTGGGCAAGAGTGCGCTGACCATCCAGCTGAACCACCAG
TGCTTCGTGGAGGACCACGACCCCACCATCCAGGATTCCTACTGGAAGGAGTTGACCCTG
GACAGTGGGGACTGCATTCTGAATGTGCTGGACACAGCAGGGCAGGCCATCCATAGGGCC
CTGCGTGACCAGTGCCTGGCTGTCTGTGATGGTGTGCTGGGCGTCTTCGCTCTCGATGAC
CCCTCGTCTCTGATCCAGCTGCAGCAGATATGGGCCACCTGGGGCCCTCACCCCGCCCAG
CCCCTTGTCCTCGTGGGCAACAAGTGTGACCTTGTGACCACTGCTGGAGATGCTCATGCC
GCTGCTGCAGCCCTCGCACACAGCTGGGGGGCCCACTTCGTGGAGACCTCGGCCAAAACA
CGGCAAGGCGTGGAGGAGGCCTTTTCCCTGCTGGTCCATGAGATCCAGAGGGTCCAGGAG
GCCATGGCGAAGGAGCCCATGGCAAGGTCCTGTAGGGAGAAGACCCGGCACCAGAAGGCC
ACCTGCCACTGTGGCTGCTCTGTGGCCGTTTAAACGAATTCGAGCTCGGTACCCGGGGAT
CCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGA
AGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAA
ACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCA
TAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTT
TGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCA
CCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGA
GTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTT
GGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGG
GGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGA
TTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAA
CAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTT
CTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGG
CTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAA
GCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCAC
CTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTT
GATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACT
CGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCG
CCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTG
ACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTC
ATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGT
GATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATC
GCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCG
GGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAA
GGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA
AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT
CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGAC
AGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCC
GACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTC
TCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG
TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGA
GTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAG
CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA
CACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAG
AGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTG
CAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTAC
GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATC
AAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATG
GCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGT
AATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCG
ATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCG
CTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTG
CCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGA
TCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACA
GAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGT
GAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCA
GGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTT
TGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGA
AGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTA
AGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCAT