NGEF ORF clone FXC09378

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Flexi ORF Clone FXC09378

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Clone info.

Species

human

Clone Name

pF1KE3911 Sequence (with vector) Restriction Map

Product ID

FXC09378

HaloTag Clone PID

FHC09378

ORF Sequence

DNA sequence (2130 bp)
Protein sequence (710 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3911

Symbol

NGEF

Alias : ARHGEF27, EPHEXIN

Description

neuronal guanine nucleotide exchange factor, transcript variant 1

Original Clone ID

cp93911

Note

We obtained this clone using the E.coli strain JM109.

Features of the cloned ORF sequence

Description

Length: 2130 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 710 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2500.1 4.8e-183 99.9 NGEF CCDS46544.1 1.3e-148 98.5 NGEF CCDS170.1 3.6e-43 35.3 ARHGEF19 CCDS34771.1 3.6e-43 38.9 ARHGEF5 CCDS46.2 7e-38 32.2 ARHGEF16 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 278 456 PF00621 Dbl homology (DH) domain IPR001452 621 665 PF00018 Src homology-3 domain HMMSmart IPR000219 277 456 SM00325 Dbl homology (DH) domain IPR001849 490 603 SM00233 Pleckstrin homology domain IPR001452 615 672 SM00326 Src homology-3 domain ProfileScan IPR000219 273 457 PS50010 Dbl homology (DH) domain IPR001849 489 601 PS50003 Pleckstrin homology domain IPR001452 612 673 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011509225 710 605991 99.9 100.0 NP_062824 710 605991 99.9 100.0 NP_001107562 618 605991 98.5 83.7 NP_694945 802 612496 35.3 92.4 XP_011539008 802 612496 35.3 92.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE3911 Download

>KIEE3911  2130 bp
ATGGAGACCAGGGAATCTGAAGATTTGGAAAAGACCCGGAGGAAATCAGCAAGTGATCAA
TGGAACACTGATAATGAACCAGCCAAGGTGAAACCTGAGTTACTCCCAGAAAAAGAGGAG
ACTTCTCAAGCTGACCAGGATATCCAAGACAAAGAGCCTCATTGCCACATCCCAATTAAG
AGAAATTCCATCTTCAATCGCTCCATAAGACGCAAAAGCAAAGCCAAGGCCAGAGACAAC
CCCGAACGGAACGCCAGCTGCCTGGCAGATTCACAGGACAATGGAAAATCTGTAAATGAG
CCCCTGACCTTGAATATCCCCTGGAGCAGAACGCCTCCTTGCAGAACAGCAATGCAGACA
GACCCAGGAGCCCAGGAAATGAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCACGCCC
GAGGAGTGGCCGGCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATC
CACCCCATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAG
GAATACCGAGATAAATCGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAA
ATAGAAGACAATACCAATGGGTCCCCGGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAA
GAGGAGGAGGAGGAGGAGCCGGCCAGCCCACCAGAGAGGAAGACTCTGCCCCAGATCTGC
CTGCTCAGTAACCCCCACTCAAGGTTCAACCTCTGGCAGGATCTTCCCGAGATCCGGAGC
AGCGGGGTGCTTGAGATCCTACAGCCTGAGGAGATTAAGCTGCAGGAGGCCATGTTCGAG
CTGGTCACTTCCGAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATG
GAGAACGAGCGGATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAAC
GTCCTGGACGTGCTGGCTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAG
GAGAACATCGTCATCTCTGACGTGTGTGACATCGTGTACCGTTATGCGGCTGACCACTTC
TCTGTCTACATCACCTACGTCAGCAATCAGACCTACCAGGAGCGGACCTATAAGCAGCTG
CTCCAGGAGAAGGCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGCTCGACCCCAAGTGC
AGGGGGCTGCCCTTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCTCAAG
CTGTTGGTCCAGAACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCT
TTGGATGCTCACAAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAA
ATGAGCCGCACGGAACAGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCG
GTGCCCATCATCTCCCACTCCCGCTGGCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCA
GGCCCCAAGACCTCCCGGACCCTGAGGACCAAGAAGCTCTTCCACGAAATTTACCTCTTC
CTGTTCAACGACCTGCTGGTGATCTGCCGGCAGATTCCAGGAGACAAGTACCAGGTATTT
GACTCAGCTCCGCGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGACGCTG
GCCAACGTGTTCATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATG
CTAAAGGCGTCCTCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGG
AGGACCAAGTTTGTTTCGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTG
CACCCATACGTGGCTCAGCAGCCAGACGAGCTGACGCTGGAGCTCGCCGACATCCTCAAC
ATCCTGGACAAGACTGACGACGGGTGGATCTTTGGCGAGCGTCTGCACGACCAGGAGAGA
GGCTGGTTCCCCAGCTCCATGACTGAGGAGATCTTGAATCCCAAGATCCGGTCCCAGAAC
CTCAAGGAATGTTTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAAGGAC
CGCAGGAAGCTGGGCAGCCGGAATCGGCAA

ORF Protein

Cloned ORF protein sequence for pF1KE3911 Download

>KIEE3911  710 aa
METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIK
RNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRTPPCRTAMQT
DPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQ
EYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQIC
LLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFM
ENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHF
SVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLK
LLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKS
VPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVF
DSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNR
RTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQER
GWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE3911 Download

>pF1KE3911  5243 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGAGACCAGG
GAATCTGAAGATTTGGAAAAGACCCGGAGGAAATCAGCAAGTGATCAATGGAACACTGAT
AATGAACCAGCCAAGGTGAAACCTGAGTTACTCCCAGAAAAAGAGGAGACTTCTCAAGCT
GACCAGGATATCCAAGACAAAGAGCCTCATTGCCACATCCCAATTAAGAGAAATTCCATC
TTCAATCGCTCCATAAGACGCAAAAGCAAAGCCAAGGCCAGAGACAACCCCGAACGGAAC
GCCAGCTGCCTGGCAGATTCACAGGACAATGGAAAATCTGTAAATGAGCCCCTGACCTTG
AATATCCCCTGGAGCAGAACGCCTCCTTGCAGAACAGCAATGCAGACAGACCCAGGAGCC
CAGGAAATGAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCACGCCCGAGGAGTGGCCG
GCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATCCACCCCATTCCC
GCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAGGAATACCGAGAT
AAATCGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAAATAGAAGACAAT
ACCAATGGGTCCCCGGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAAGAGGAGGAGGAG
GAGGAGCCGGCCAGCCCACCAGAGAGGAAGACTCTGCCCCAGATCTGCCTGCTCAGTAAC
CCCCACTCAAGGTTCAACCTCTGGCAGGATCTTCCCGAGATCCGGAGCAGCGGGGTGCTT
GAGATCCTACAGCCTGAGGAGATTAAGCTGCAGGAGGCCATGTTCGAGCTGGTCACTTCC
GAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATGGAGAACGAGCGG
ATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAACGTCCTGGACGTG
CTGGCTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAGGAGAACATCGTC
ATCTCTGACGTGTGTGACATCGTGTACCGTTATGCGGCTGACCACTTCTCTGTCTACATC
ACCTACGTCAGCAATCAGACCTACCAGGAGCGGACCTATAAGCAGCTGCTCCAGGAGAAG
GCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGCTCGACCCCAAGTGCAGGGGGCTGCCC
TTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCTCAAGCTGTTGGTCCAG
AACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCTTTGGATGCTCAC
AAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAAATGAGCCGCACG
GAACAGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTGCCCATCATC
TCCCACTCCCGCTGGCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCAGGCCCCAAGACC
TCCCGGACCCTGAGGACCAAGAAGCTCTTCCACGAAATTTACCTCTTCCTGTTCAACGAC
CTGCTGGTGATCTGCCGGCAGATTCCAGGAGACAAGTACCAGGTATTTGACTCAGCTCCG
CGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGACGCTGGCCAACGTGTTC
ATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATGCTAAAGGCGTCC
TCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTT
GTTTCGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTGCACCCATACGTG
GCTCAGCAGCCAGACGAGCTGACGCTGGAGCTCGCCGACATCCTCAACATCCTGGACAAG
ACTGACGACGGGTGGATCTTTGGCGAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCC
AGCTCCATGACTGAGGAGATCTTGAATCCCAAGATCCGGTCCCAGAACCTCAAGGAATGT
TTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAAGGACCGCAGGAAGCTG
GGCAGCCGGAATCGGCAAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAG
TCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTT
GGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTT
GAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGC
CCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGC
GTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTG
CGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCG
GCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCC
TGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGA
TCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCA
GGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATC
GGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTC
AAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGG
CTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGG
GACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCT
GCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCT
ACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAA
GCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAA
CTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGC
GATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGT
GGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCT
GAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCC
GATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGG
GGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAAT
ACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA
AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCC
TGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATA
AAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC
GCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTC
ACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA
ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCC
GGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAG
GACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAG
CTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA
GATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGA
CGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGAT
CTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGC
TGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCC
TGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCA
GTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCG
TTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAG
CCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT
TTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCC
TGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCG
TAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAA
TAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGA
ACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGC
CCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGG
CCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCAT