SPG7 ORF clone FXC03711

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Flexi ORF Clone FXC03711

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Clone info.

Species

human

Clone Name

pF1KB5737 Sequence (with vector) Restriction Map

Product ID

FXC03711

HaloTag Clone PID

FHC03711

ORF Sequence

DNA sequence (2385 bp)
Protein sequence (795 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527784]

Gene

KIBB5737

Symbol

SPG7

Alias : CAR, CMAR, PGN, SPG5C

Description

spastic paraplegia 7 (pure and complicated autosomal recessive), transcript variant 1

Original Clone ID

cs01732

Features of the cloned ORF sequence

Description

Length: 2385 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 795 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10977.1 0 100.0 SPG7 CCDS10978.1 5.9e-134 100.0 SPG7 CCDS11859.1 6.8e-80 45.6 AFG3L2 CCDS58072.1 1.1e-42 38.6 YME1L1 CCDS7151.1 1.1e-42 38.6 YME1L1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011546 144 239 PF06480 Peptidase M41 IPR003959 346 478 PF00004 ATPase IPR000642 544 746 PF01434 Peptidase M41 HMMSmart IPR003593 341 481 SM00382 ATPase HMMTigr IPR005936 265 747 TIGR01241 Peptidase M41
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 143 RTLLVIAVVMSLLNALSTSGGSI 165 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003110 795 602783,607259 100.0 100.0 XP_006721327 809 602783,607259 99.9 91.7 NP_006787 797 604581,610246,614487 45.6 82.6 XP_011523903 730 604581,610246,614487 39.3 82.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KB5737 Download

>KIBB5737  2385 bp
ATGGCCGTGCTGCTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGG
CCGCTGTGGGGCCCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGG
CGGCCGTACATGGCTAGCAGGCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTG
CAGAGCTTACAATTGAGACTGCTAACCCCTACCTTTGAAGGGATCAACGGATTGTTGTTG
AAACAACATTTAGTTCAGAATCCAGTCAGACTCTGGCAACTTTTAGGTGGTACTTTCTAT
TTTAACACCTCAAGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAAGGGGAAGGCG
CCTGAAGAGGACGAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAG
CGGCTGCGCACCTTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACC
AGCGGAGGCAGCATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTG
CAGCGCGTCCAGGTGGTGCCTGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCC
GTGGTGTTTGGGCGGCCTCGGCTAGCCTTGATGTACCGAATGCAGGTTGCAAATATTGAC
AAGTTTGAAGAGAAGCTTCGAGCAGCTGAAGATGAGCTGAATATCGAGGCCAAGGACAGG
ATCCCAGTTTCCTACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTCTGTGGGGATG
ACGGCAGTGGGCCTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGG
GAAGGTGGATTCAGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGG
AAGATGGGGAAAGGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAA
GTCCGCGAGTTTGTGGATTATCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAG
GTCCCAAAGGGCGCACTGCTGCTCGGCCCCCCCGGCTGTGGGAAGACGCTGCTGGCCAAG
GCGGTGGCCACGGAGGCTCAGGTGCCCTTCCTGGCGATGGCCGGCCCAGAGTTCGTGGAG
GTCATTGGAGGCCTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGCCCGAGCCCGG
GCCCCCTGCATCGTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACC
ATGTCCGGCTTCTCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATG
GATGGAATGGGTACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATT
TTGGACGGTGCTCTGATGAGGCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCC
ACGCTGCAGGAGAGGCGGGAGATTTTTGAGCAGCACCTGAAGAGCCTGAAGCTGACCCAG
TCCAGCACCTTTTACTCCCAGCGTCTGGCAGAGCTGACACCAGGATTCAGTGGGGCTGAC
ATCGCCAACATCTGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACACACTTCCGTG
CACACTCTCAACTTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGC
AAGATCCTGTCCAAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTG
GTGGGCTGGATGCTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACA
AACGCCGCCCTGGGCTTTGCTCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAG
CAGCTGTTTGAGCGGATGTGCATGGCCCTGGGAGGACGGGCCTCGGAAGCACTGTCCTTC
AACGAGGTCACTTCTGGGGCACAGGACGACCTGAGGAAGGTCACCCGCATCGCCTACTCC
ATGGTGAAGCAGTTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCCTGAGGCGCAG
GAGGGCCTCATGGGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGAC
CATGAAGCAAGACTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGAC
AACCTGGACAAGTTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTAT
GAGGACATTGAGGCTCTCATTGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCG
CAGAGGTGGATCGACGCCCAGAGGGAGAAACAGGACTTGGGCGAGGAGGAGACCGAAGAG
ACCCAGCAGCCTCCACTTGGAGGCGAAGAGCCGACTTGGCCCAAG

ORF Protein

Cloned ORF protein sequence for pF1KB5737 Download

>KIBB5737  795 aa
MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRAL
QSLQLRLLTPTFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKA
PEEDEEERRRRERDDQMYRERLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEV
QRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEAKDR
IPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDG
KMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAK
AVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTT
MSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADILDGALMRPGRLDRHVFIDLP
TLQERREIFEQHLKSLKLTQSSTFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSV
HTLNFEYAVERVLAGTAKKSKILSKEEQKVVAFHESGHALVGWMLEHTEAVMKVSITPRT
NAALGFAQMLPRDQHLFTKEQLFERMCMALGGRASEALSFNEVTSGAQDDLRKVTRIAYS
MVKQFGMAPGIGPISFPEAQEGLMGIGRRPFSQGLQQMMDHEARLLVAKAYRHTEKVLQD
NLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQRWIDAQREKQDLGEEETEE
TQQPPLGGEEPTWPK

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB5737 Download

>pF1KB5737  5498 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGCCGTGCTG
CTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGGGC
CCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATG
GCTAGCAGGCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAA
TTGAGACTGCTAACCCCTACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTA
GTTCAGAATCCAGTCAGACTCTGGCAACTTTTAGGTGGTACTTTCTATTTTAACACCTCA
AGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAAGGGGAAGGCGCCTGAAGAGGAC
GAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAGCGGCTGCGCACC
TTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCAGC
ATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAG
GTGGTGCCTGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCCGTGGTGTTTGGG
CGGCCTCGGCTAGCCTTGATGTACCGAATGCAGGTTGCAAATATTGACAAGTTTGAAGAG
AAGCTTCGAGCAGCTGAAGATGAGCTGAATATCGAGGCCAAGGACAGGATCCCAGTTTCC
TACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTCTGTGGGGATGACGGCAGTGGGC
CTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGGGAAGGTGGATTC
AGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGGAAGATGGGGAAA
GGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAAGTCCGCGAGTTT
GTGGATTATCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAGGTCCCAAAGGGC
GCACTGCTGCTCGGCCCCCCCGGCTGTGGGAAGACGCTGCTGGCCAAGGCGGTGGCCACG
GAGGCTCAGGTGCCCTTCCTGGCGATGGCCGGCCCAGAGTTCGTGGAGGTCATTGGAGGC
CTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGCCCGAGCCCGGGCCCCCTGCATC
GTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACCATGTCCGGCTTC
TCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATGGATGGAATGGGT
ACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATTTTGGACGGTGCT
CTGATGAGGCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCCACGCTGCAGGAG
AGGCGGGAGATTTTTGAGCAGCACCTGAAGAGCCTGAAGCTGACCCAGTCCAGCACCTTT
TACTCCCAGCGTCTGGCAGAGCTGACACCAGGATTCAGTGGGGCTGACATCGCCAACATC
TGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACACACTTCCGTGCACACTCTCAAC
TTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGCAAGATCCTGTCC
AAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTGGTGGGCTGGATG
CTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACAAACGCCGCCCTG
GGCTTTGCTCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAGCAGCTGTTTGAG
CGGATGTGCATGGCCCTGGGAGGACGGGCCTCGGAAGCACTGTCCTTCAACGAGGTCACT
TCTGGGGCACAGGACGACCTGAGGAAGGTCACCCGCATCGCCTACTCCATGGTGAAGCAG
TTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCCTGAGGCGCAGGAGGGCCTCATG
GGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGACCATGAAGCAAGA
CTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGACAACCTGGACAAG
TTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAG
GCTCTCATTGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCGCAGAGGTGGATC
GACGCCCAGAGGGAGAAACAGGACTTGGGCGAGGAGGAGACCGAAGAGACCCAGCAGCCT
CCACTTGGAGGCGAAGAGCCGACTTGGCCCAAGGTTTAAACGAATTCGAGCTCGGTACCC
GGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCG
AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGC
CTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGC
TGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTT
TCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGG
TCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCG
CCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGT
AAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGG
CGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAA
GATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGG
GCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC
CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCA
GCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC
ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCA
TCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCAT
ACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCA
CGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGG
CTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTC
GTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCT
GGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT
ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTAC
GGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTC
TGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCA
CTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTG
AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCA
TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAA
CCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC
TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC
GCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT
GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG
TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAG
GATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA
CGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGG
AAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTT
TGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTT
TTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAG
ATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGC
TGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGT
GGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCA
GGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTG
ATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTAC
TGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGT
AGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGAT
AAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTC
AGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAA
CTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCT
GTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACG
TTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATC
AAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT