PUS7L ORF clone FHC07798

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC07798

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Clone info.

Species

human

Clone Name

pFN21AE0005 Sequence (with vector) Restriction Map

Product ID

FHC07798

Flexi Clone PID

FXC07798

ORF Sequence

DNA sequence (2103 bp)
Protein sequence (701 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527251]

Gene

KIEE0005

Symbol

PUS7L

Description

pseudouridylate synthase 7-like, transcript variant 3

Original Clone ID

oc00705

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2103 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 701 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8743.1 0 100.0 PUS7L CCDS61104.1 2.4e-162 100.0 PUS7L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001656 293 696 PF01142 Pseudouridine synthase HMMTigr IPR001656 301 700 TIGR00094 Pseudouridine synthase ProfileScan IPR011760 424 647 PS50984 Pseudouridine synthase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0005 Download

>KIEE0005  2103 bp
ATGGAAGAAGATACAGATTATAGAATCAGGTTTAGTTCTTTGTGTTTCTTTAATGATCAC
GTTGGATTTCATGGCACTATAAAAAGCTCACCAAGTGACTTTATTGTTATTGAAATTGAT
GAACAGGGACAGTTAGTTAATAAGACCATCGATGAGCCTATTTTCAAGATTAGTGAAATA
CAACTTGAGCCAAATAATTTTCCCAAAAAACCAAAACTAGATCTTCAAAATCTGTCCTTA
GAAGATGGAAGAAACCAAGAAGTTCATACTTTGATTAAGTACACTGATGGTGACCAAAAT
CATCAGTCTGGTTCAGAAAAGGAAGATACTATCGTTGATGGAACTTCCAAATGTGAAGAA
AAAGCTGATGTTTTAAGCTCCTTTTTGGATGAAAAAACTCATGAGTTACTGAATAATTTT
GCCTGTGATGTAAGAGAGAAGTGGCTTTCTAAAACAGAGCTAATTGGACTACCTCCTGAA
TTCTCAATAGGCAGAATCCTTGACAAAAACCAGAGGGCTAGTTTACACAGTGCCATTAGG
CAGAAATTTCCATTTTTAGTAACTGTAGGAAAAAACAGTGAAATTGTTGTAAAACCAAAT
CTTGAATATAAAGAACTTTGTCATTTGGTATCTGAAGAGGAAGCATTTGACTTTTTTAAA
TATTTGGATGCAAAGAAAGAAAATTCCAAATTTACCTTTAAACCTGATACAAACAAAGAC
CACAGAAAAGCTGTCCACCATTTTGTCAACAAAAAGTTTGGAAACCTTGTGGAAACCAAA
TCTTTTTCTAAAATGAATTGCAGTGCTGGTAATCCGAATGTGGTGGTAACAGTAAGATTT
CGGGAAAAAGCACACAAACGTGGGAAAAGGCCTCTTTCTGAATGCCAAGAAGGAAAAGTT
ATATATACAGCTTTTACCCTACGAAAGGAAAACCTGGAAATGTTTGAAGCGATTGGTTTT
TTAGCTATCAAACTTGGTGTTATTCCTTCGGATTTTAGTTATGCAGGCCTTAAAGACAAG
AAAGCCATCACCTATCAAGCAATGGTTGTTAGAAAAGTGACTCCAGAGAGGTTGAAAAAT
ATTGAAAAAGAAATTGAAAAGAAAAGAATGAATGTCTTTAATATTCGGTCTGTAGATGAT
TCCCTGAGACTTGGTCAGCTCAAAGGAAATCACTTTGATATTGTCATTAGAAATTTAAAA
AAACAAATAAATGATTCTGCAAACCTGAGGGAGAGAATTATGGAAGCAATAGAAAATGTT
AAGAAAAAAGGCTTTGTGAATTACTATGGACCACAGAGATTTGGGAAGGGAAGGAAAGTT
CACACAGACCAAATTGGACTAGCTTTGCTGAAGAATGAAATGATGAAAGCCATAAAATTG
TTTCTTACACCAGAAGACTTGGATGATCCTGTAAATAGAGCAAAGAAGTATTTTCTTCAA
ACTGAGGATGCTAAAGGCACACTTTCATTGATGCCTGAATTCAAAGTGCGTGAGAGAGCA
TTGTTGGAGGCATTGCACCGCTTTGGCATGACCGAGGAAGGTTGTATCCAGGCATGGTTC
TCTTTACCCCATTCCATGCGCATATTCTATGTTCACGCATATACCAGCAAAATTTGGAAT
GAGGCAGTATCTTACAGACTTGAAACCTATGGAGCAAGAGTAGTGCAGGGTGATTTGGTC
TGTTTGGATGAAGACATTGATGACGAGAATTTCCCAAATAGTAAAATTCACCTGGTAACT
GAAGAGGAGGGATCAGCTAATATGTATGCAATACATCAGGTGGTTCTTCCAGTACTTGGA
TACAATATTCAGTACCCGAAGAACAAAGTAGGGCAGTGGTACCATGACATACTTAGCAGA
GATGGACTACAGACATGTAGGTTTAAAGTACCTACTCTGAAACTGAATATACCAGGTTGC
TATAGACAGATTTTGAAACATCCCTGTAATCTCTCATACCAACTAATGGAAGATCATGAC
ATTGATGTCAAAACGAAAGGTTCCCACATTGATGAAACAGCTTTGTCTCTTTTGATCTCT
TTTGATCTTGATGCTTCATGCTATGCTACCGTTTGTCTGAAGGAAATAATGAAGCATGAC
GTT

ORF Protein

Cloned ORF protein sequence for pFN21AE0005 Download

>KIEE0005  701 aa
MEEDTDYRIRFSSLCFFNDHVGFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEI
QLEPNNFPKKPKLDLQNLSLEDGRNQEVHTLIKYTDGDQNHQSGSEKEDTIVDGTSKCEE
KADVLSSFLDEKTHELLNNFACDVREKWLSKTELIGLPPEFSIGRILDKNQRASLHSAIR
QKFPFLVTVGKNSEIVVKPNLEYKELCHLVSEEEAFDFFKYLDAKKENSKFTFKPDTNKD
HRKAVHHFVNKKFGNLVETKSFSKMNCSAGNPNVVVTVRFREKAHKRGKRPLSECQEGKV
IYTAFTLRKENLEMFEAIGFLAIKLGVIPSDFSYAGLKDKKAITYQAMVVRKVTPERLKN
IEKEIEKKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENV
KKKGFVNYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNRAKKYFLQ
TEDAKGTLSLMPEFKVRERALLEALHRFGMTEEGCIQAWFSLPHSMRIFYVHAYTSKIWN
EAVSYRLETYGARVVQGDLVCLDEDIDDENFPNSKIHLVTEEEGSANMYAIHQVVLPVLG
YNIQYPKNKVGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQILKHPCNLSYQLMEDHD
IDVKTKGSHIDETALSLLISFDLDASCYATVCLKEIMKHDV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0005 Download

>pFN21AE0005  6808 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAGAAGATACAGATTATAGAATCAGGTTTAG
TTCTTTGTGTTTCTTTAATGATCACGTTGGATTTCATGGCACTATAAAAAGCTCACCAAG
TGACTTTATTGTTATTGAAATTGATGAACAGGGACAGTTAGTTAATAAGACCATCGATGA
GCCTATTTTCAAGATTAGTGAAATACAACTTGAGCCAAATAATTTTCCCAAAAAACCAAA
ACTAGATCTTCAAAATCTGTCCTTAGAAGATGGAAGAAACCAAGAAGTTCATACTTTGAT
TAAGTACACTGATGGTGACCAAAATCATCAGTCTGGTTCAGAAAAGGAAGATACTATCGT
TGATGGAACTTCCAAATGTGAAGAAAAAGCTGATGTTTTAAGCTCCTTTTTGGATGAAAA
AACTCATGAGTTACTGAATAATTTTGCCTGTGATGTAAGAGAGAAGTGGCTTTCTAAAAC
AGAGCTAATTGGACTACCTCCTGAATTCTCAATAGGCAGAATCCTTGACAAAAACCAGAG
GGCTAGTTTACACAGTGCCATTAGGCAGAAATTTCCATTTTTAGTAACTGTAGGAAAAAA
CAGTGAAATTGTTGTAAAACCAAATCTTGAATATAAAGAACTTTGTCATTTGGTATCTGA
AGAGGAAGCATTTGACTTTTTTAAATATTTGGATGCAAAGAAAGAAAATTCCAAATTTAC
CTTTAAACCTGATACAAACAAAGACCACAGAAAAGCTGTCCACCATTTTGTCAACAAAAA
GTTTGGAAACCTTGTGGAAACCAAATCTTTTTCTAAAATGAATTGCAGTGCTGGTAATCC
GAATGTGGTGGTAACAGTAAGATTTCGGGAAAAAGCACACAAACGTGGGAAAAGGCCTCT
TTCTGAATGCCAAGAAGGAAAAGTTATATATACAGCTTTTACCCTACGAAAGGAAAACCT
GGAAATGTTTGAAGCGATTGGTTTTTTAGCTATCAAACTTGGTGTTATTCCTTCGGATTT
TAGTTATGCAGGCCTTAAAGACAAGAAAGCCATCACCTATCAAGCAATGGTTGTTAGAAA
AGTGACTCCAGAGAGGTTGAAAAATATTGAAAAAGAAATTGAAAAGAAAAGAATGAATGT
CTTTAATATTCGGTCTGTAGATGATTCCCTGAGACTTGGTCAGCTCAAAGGAAATCACTT
TGATATTGTCATTAGAAATTTAAAAAAACAAATAAATGATTCTGCAAACCTGAGGGAGAG
AATTATGGAAGCAATAGAAAATGTTAAGAAAAAAGGCTTTGTGAATTACTATGGACCACA
GAGATTTGGGAAGGGAAGGAAAGTTCACACAGACCAAATTGGACTAGCTTTGCTGAAGAA
TGAAATGATGAAAGCCATAAAATTGTTTCTTACACCAGAAGACTTGGATGATCCTGTAAA
TAGAGCAAAGAAGTATTTTCTTCAAACTGAGGATGCTAAAGGCACACTTTCATTGATGCC
TGAATTCAAAGTGCGTGAGAGAGCATTGTTGGAGGCATTGCACCGCTTTGGCATGACCGA
GGAAGGTTGTATCCAGGCATGGTTCTCTTTACCCCATTCCATGCGCATATTCTATGTTCA
CGCATATACCAGCAAAATTTGGAATGAGGCAGTATCTTACAGACTTGAAACCTATGGAGC
AAGAGTAGTGCAGGGTGATTTGGTCTGTTTGGATGAAGACATTGATGACGAGAATTTCCC
AAATAGTAAAATTCACCTGGTAACTGAAGAGGAGGGATCAGCTAATATGTATGCAATACA
TCAGGTGGTTCTTCCAGTACTTGGATACAATATTCAGTACCCGAAGAACAAAGTAGGGCA
GTGGTACCATGACATACTTAGCAGAGATGGACTACAGACATGTAGGTTTAAAGTACCTAC
TCTGAAACTGAATATACCAGGTTGCTATAGACAGATTTTGAAACATCCCTGTAATCTCTC
ATACCAACTAATGGAAGATCATGACATTGATGTCAAAACGAAAGGTTCCCACATTGATGA
AACAGCTTTGTCTCTTTTGATCTCTTTTGATCTTGATGCTTCATGCTATGCTACCGTTTG
TCTGAAGGAAATAATGAAGCATGACGTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT