TSSK1B ORF clone FHC02252

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Halotag ORF Clone FHC02252

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Clone info.

Species

human

Clone Name

pFN21AB7102 Sequence (with vector) Restriction Map

Product ID

FHC02252

Flexi Clone PID

FXC02252

ORF Sequence

DNA sequence (651 bp)
Protein sequence (217 aa)

Linker Type

Flexi type

more Linker info

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site (GTTTAAAC) is tagged at the 3' end of the ORF instead of the stop codon. The resultant sequence encodes Val-stop in place of stop. By cutting the sequence using Pme I, the stop codon arising in the Pme I site can be omitted.

Vector Type

pFN21A Vector sequence Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464426]

Gene

KIBB7102

Symbol

TSSK1B

Alias : FKSG81, SPOGA4, STK22D, TSK1, TSSK1

Description

testis-specific serine kinase 1B

Original Clone ID

cp01107

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 651 bp

Restriction map A Restriction map B

Genomic Structure

Features of the protein sequence

Description

Length: 217 aa

Result of homology search against CCDS protein database ( FASTA output, Multiple alignment)

no hit
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7102 Download

>KIBB7102  651 bp
ATGTGGGTTACCAAGTTCAAGGGGAGAGAGAAGAAGCTGATGAAAATAGAGGCTCATCTG
GGACTGCCTTCCTTCTCTGGCTCCACCCTTGACTTCTTCAGAGCTTGGGCTTTAAGCCGT
GAGCTCCATCTCATTCCCTGGGCCGCAAGAAGCTCACTGGGCCCGCGTCTCTGGAGGCTG
TTGGGGGGGCCCTTCCTCTGTCTCCATAGTCGACGGCTTGCTGGGGAAACCCAGGATCTC
CGACTGCCTGGACATTTGCATTGCTGTCCCCTCGGGTTTTGTCTCAGGCTGTGCCTGGGG
CTGTGCCTCTCCCTCAGGCTCCAGCTTGGTGGCAGACTTCTTGTCAGAGCCAGGTTCGGG
GGTCCACAAGGGTTCAGTTCCCCGGGAACTCTCCCCCTCCTTGTTGATGGCCACAGAGGG
AGATCCCCGTGCCTTGGGCTGCATCCAGCAGTGGCTGAGGATCTCGTCGATGTGGAGCCG
CCGGTTGACGTCGGGCTGCAGCATGTGGTAGATGAGGTCCTTGCACTCGCCTGTCAGGTG
CTTGGAGCGTGGGAAGTTGACGCGGTGCTCCTTCTGGATACGCAGCATCTTCTTGATGTT
GGAGTCGTCGTAGGGCATGGAGCCGCAGACCATGATGTAGAGGATCACGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7102 Download

>KIBB7102  217 aa
MWVTKFKGREKKLMKIEAHLGLPSFSGSTLDFFRAWALSRELHLIPWAARSSLGPRLWRL
LGGPFLCLHSRRLAGETQDLRLPGHLHCCPLGFCLRLCLGLCLSLRLQLGGRLLVRARFG
GPQGFSSPGTLPLLVDGHRGRSPCLGLHPAVAEDLVDVEPPVDVGLQHVVDEVLALACQV
LGAWEVDAVLLLDTQHLLDVGVVVGHGAADHDVEDHA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7102 Download

>pFN21AB7102  5356 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGGGTTACCAAGTTCAAGGGGAGAGAGAAGAA
GCTGATGAAAATAGAGGCTCATCTGGGACTGCCTTCCTTCTCTGGCTCCACCCTTGACTT
CTTCAGAGCTTGGGCTTTAAGCCGTGAGCTCCATCTCATTCCCTGGGCCGCAAGAAGCTC
ACTGGGCCCGCGTCTCTGGAGGCTGTTGGGGGGGCCCTTCCTCTGTCTCCATAGTCGACG
GCTTGCTGGGGAAACCCAGGATCTCCGACTGCCTGGACATTTGCATTGCTGTCCCCTCGG
GTTTTGTCTCAGGCTGTGCCTGGGGCTGTGCCTCTCCCTCAGGCTCCAGCTTGGTGGCAG
ACTTCTTGTCAGAGCCAGGTTCGGGGGTCCACAAGGGTTCAGTTCCCCGGGAACTCTCCC
CCTCCTTGTTGATGGCCACAGAGGGAGATCCCCGTGCCTTGGGCTGCATCCAGCAGTGGC
TGAGGATCTCGTCGATGTGGAGCCGCCGGTTGACGTCGGGCTGCAGCATGTGGTAGATGA
GGTCCTTGCACTCGCCTGTCAGGTGCTTGGAGCGTGGGAAGTTGACGCGGTGCTCCTTCT
GGATACGCAGCATCTTCTTGATGTTGGAGTCGTCGTAGGGCATGGAGCCGCAGACCATGA
TGTAGAGGATCACGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT